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Survival in its intracellular niche of the ileum epithelial lining requires the capacity to subvert, repress or exploit the host immune response to create an environment conducive to bacterial propagation. To better understand how <jats:italic>L. intracellularis<\/jats:italic> survives in its intracellular niche, we have performed an investigation into the dynamic relationship between infection and the host autophagy response by immunohistochemistry in experimentally infected porcine ileum samples.<\/jats:p><jats:p>Beclin1, a protein required early in the autophagy pathway was observed to be distributed with a basal to apical concentration gradient in the crypts of healthy piglets, whilst infected piglets were observed to have no gradient of distribution and an increase in the presence of Beclin1 in crypts with histological characteristics of <jats:italic>L. intracellularis<\/jats:italic> residence. Detecting microtubule-associated protein light chain 3 (LC3) is used as a method for monitoring autophagy progression as it associates with mature autophagosomes. For LC3 there was no notable change in signal intensity between crypts with characteristic <jats:italic>L. intracellularis<\/jats:italic> infection and healthy crypts of uninfected pigs. Finally, as p62 is degraded with the internal substrate of an autophagosome it was used to measure autophagic flux. There was no observed reduction or redistribution of p62.<\/jats:p><jats:p>These preliminary results of the autophagy response in the ileum suggest that <jats:italic>L. intracellularis<\/jats:italic> affects autophagy. This disruption to host ileum homeostasis may provide a mechanism that assists in bacterial propagation and contributes to pathogenesis.<\/jats:p>","DOI":"10.1007\/s11259-021-09847-7","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T17:56:55Z","timestamp":1634752615000},"page":"585-592","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis"],"prefix":"10.1007","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0182-6210","authenticated-orcid":false,"given":"Hamish A.","family":"Salvesen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5160-4208","authenticated-orcid":false,"given":"Fiona A.","family":"Sargison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9213-1830","authenticated-orcid":false,"given":"Alan L.","family":"Archibald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4849-0665","authenticated-orcid":false,"given":"Tahar","family":"Ait-Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,20]]},"reference":[{"key":"9847_CR1","doi-asserted-by":"publisher","first-page":"1050","DOI":"10.1016\/j.celrep.2017.07.019","volume":"20","author":"J Asano","year":"2017","unstructured":"Asano J, Sato T, Ichinose S, Kajita M, Onai N, Shimizu S, Correspondence TO (2017) Intrinsic Autophagy Is Required for the Maintenance of Intestinal Stem Cells and for Irradiation-Induced Intestinal Regeneration. 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As only paraffin blocks were used, institutional ethics committee approval was unnecessary for this current work.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Not Applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"All authors have consented to the publication and presentation of the data in this article.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no potential conflicts of interest with respect to the research, authorship and\/or publication of this article.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflicts interests"}}]}}