{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T07:47:11Z","timestamp":1768636031125,"version":"3.49.0"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004687","name":"Universidad de Murcia","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004687","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract\n<\/jats:title><jats:p>This work studied the effects of the inclusion of Purple Garlic Powder (PGP) and Oregano Essential Oil (OEO) in the feed, at different doses and combinations, on intestinal health and the growth performance of 140 and 3000 piglets, respectively, weaned at 21\u00a0days of age. Seven dietary treatments were used: a negative control group (basal diet), a positive control group with ZnO (3000\u00a0mg\/Kg of feed), two groups with OEO at 0.4% and 1.2% respectively, two groups with PGP at 0.4% and 2% respectively and one group with OEO at 1.2% combined with PGP at 2%. Only the positive control group received ZnO in the diet. Each group of piglets received the treatment for seven weeks, from weaning, and were later sacrificed to obtain jejunum and ileum samples for counting of goblet cells, intraepithelial lymphocytes, and IgA-producing cells. The growth performance were measured at the beginning and at the end of the seven weeks. In jejunum and ileum, the number of goblet cells increased in the groups with ZnO, PGP 2%, OEO 1.2% and PGP 2%\u2009+\u2009OEO 1.2%, presenting significant differences with the rest of the groups. The results obtained for the intraepithelial lymphocyte count were in line with those obtained for the count of goblet cells. Regarding IgA-producing cells, the groups that showed significantly favourable results in the jejunum and ileum were OEO 1.2%, PGP 2% and their combination, but the groups that showed the most similar means to ZnO were the OEO 0.4% and the PGP 0.4%. Regarding the growth performance, PGP 2%, OEO 1.2% and their combination had similar results to ZnO. The intestinal health of piglets could be improved, without harming the growth performance, by means of the supplementation of PGP 2%, OEO 1.2% and PGP 2%\u2009+\u2009OEO 1.2% offering a natural alternative to the use of ZnO.<\/jats:p>","DOI":"10.1007\/s11259-022-10053-2","type":"journal-article","created":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T13:04:53Z","timestamp":1671109493000},"page":"901-909","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of dietary supplementation with purple garlic powder and oregano essential oil on intestinal health in post-weaning piglets from commercial farms"],"prefix":"10.1007","volume":"47","author":[{"given":"Daniel","family":"Serrano-Jara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Rivera-Gomis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Antonio","family":"Tornel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Bernab\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Mart\u00ednez-Conesa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Antonio","family":"Navarro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"C\u00e1novas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Otal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Cubero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,12,15]]},"reference":[{"key":"10053_CR1","first-page":"77","volume":"32","author":"V Agull\u00f3","year":"2016","unstructured":"Agull\u00f3 V, Miralles A, Bernab\u00e9 A, Cubero MJ (2016) Efectos sanitarios y productivos del ajo morado en avicultura industrial y ecol\u00f3gica. An Vet Mur 32:77\u201379","journal-title":"An Vet Mur"},{"key":"10053_CR2","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1016\/j.theriogenology.2003.06.010","volume":"63","author":"P Allan","year":"2005","unstructured":"Allan P, Bilkei G (2005) Oregano improves reproductive performance of sows. Theriogenology 63:716\u2013721. https:\/\/doi.org\/10.1016\/j.theriogenology.2003.06.010","journal-title":"Theriogenology"},{"key":"10053_CR3","doi-asserted-by":"publisher","first-page":"716S","DOI":"10.1093\/jn\/136.3.716s","volume":"136","author":"H Amagase","year":"2006","unstructured":"Amagase H (2006) Clarifying the real bioactive constituents of garlic. J Nutr 136:716S-725S. https:\/\/doi.org\/10.1093\/jn\/136.3.716s","journal-title":"J Nutr"},{"key":"10053_CR4","doi-asserted-by":"publisher","first-page":"955S","DOI":"10.1093\/jn\/131.3.955s","volume":"131","author":"H Amagase","year":"2001","unstructured":"Amagase H, Petesch BL, Matsuura H, Kasuga S, Itakura Y (2001) Intake of garlic and its bioactive components. J Nutr 131:955S-962S. https:\/\/doi.org\/10.1093\/jn\/131.3.955s","journal-title":"J Nutr"},{"key":"10053_CR5","unstructured":"Armocida A, Valette E (2019) Salud intestinal en el cerdo. Vetanco. https:\/\/www.vetanco.com\/es\/wp-content\/uploads\/sites\/3\/2019\/04\/A.C.-SALUD-INSTESTINAL-DEL-CERDO.pdf. Accesed 16 Jan 2021"},{"key":"10053_CR6","doi-asserted-by":"publisher","first-page":"1299","DOI":"10.1021\/jf0402375","volume":"53","author":"S Ayd\u0131n","year":"2005","unstructured":"Ayd\u0131n S, Ba\u015faran AA, Ba\u015faran N, Aydin S (2005) Modulating effects of thyme and its major ingredients on oxidative DNA damage in human lymphocytes. J Agric Food Chem 53:1299\u20131305. https:\/\/doi.org\/10.1021\/jf0402375","journal-title":"J Agric Food Chem"},{"key":"10053_CR7","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1111\/1541-4337.12179","volume":"15","author":"AM Bakry","year":"2015","unstructured":"Bakry AM, Abbas S, Ali B, Majeed H, Abouelwafa MY, Mousa A, Liang L (2015) Microencapsulation of oils: A comprehensive review of benefits, techniques, and applications. Compr Rev Food Sci Food Saf 15:143\u2013182. https:\/\/doi.org\/10.1111\/1541-4337.12179","journal-title":"Compr Rev Food Sci Food Saf"},{"key":"10053_CR8","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/j.vetimm.2014.05.014","volume":"160","author":"C Bianco","year":"2014","unstructured":"Bianco C, Felice V, Panarese S, Marrocco R, Ostanello F, Brunetti B, Muscatello LV, Leotti G, Vila T, Joisel F (2014) Quantitative immunohistochemical assessment of IgA, IgM, IgG and antigen-specific immunoglobulin secreting plasma cells in pig small intestinal lamina propria. Vet Immunol Immunopathol 160:281\u2013287. https:\/\/doi.org\/10.1016\/j.vetimm.2014.05.014","journal-title":"Vet Immunol Immunopathol"},{"key":"10053_CR9","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1038\/mi.2007.9","volume":"1","author":"P Brandtzaeg","year":"2008","unstructured":"Brandtzaeg P, Kiyono H, Pabst R, Russell MW (2008) Terminology: nomenclature of mucosa-associated lymphoid tissue. Mucosal Immunol 1:31\u201337. https:\/\/doi.org\/10.1038\/mi.2007.9","journal-title":"Mucosal Immunol"},{"key":"10053_CR10","doi-asserted-by":"publisher","first-page":"3605","DOI":"10.1128\/aac.00058-10","volume":"54","author":"LM Cavaco","year":"2010","unstructured":"Cavaco LM, Hasman H, Stegger M, Andersen PS, Skov R, Fluit AC, Ito T, Aarestrup FM (2010) Cloning and occurrence of czrC, a gene conferring cadmium and zinc resistance in methicillin-resistant Staphylococcus aureus CC398 isolates. Antimi-Crob Agents Chemother 54:3605\u20133608. https:\/\/doi.org\/10.1128\/aac.00058-10","journal-title":"Antimi-Crob Agents Chemother"},{"key":"10053_CR11","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/j.vetmic.2011.02.014","volume":"150","author":"LM Cavaco","year":"2011","unstructured":"Cavaco LM, Hasman H, Aarestrup FM (2011) Zinc resistance of Staphylococcus aureus of animal origin is strongly associated with methicillin resistance. Veter Microbiol 150:344\u2013348. https:\/\/doi.org\/10.1016\/j.vetmic.2011.02.014","journal-title":"Veter Microbiol"},{"key":"10053_CR12","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1111\/j.1745-4514.2000.tb00715.x","volume":"24","author":"G Cervato","year":"2000","unstructured":"Cervato G, Carabelli M, Gervasio S, Cittera A, Cazzola R, Cestaro B (2000) Antioxidant properties of oregano (Origanum vulgare) leaf extracts. J Food Biochem 24:453\u2013465. https:\/\/doi.org\/10.1111\/j.1745-4514.2000.tb00715.x","journal-title":"J Food Biochem"},{"key":"10053_CR13","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.4315\/0362-028x-58.10.1147","volume":"58","author":"RK Daouk","year":"1995","unstructured":"Daouk RK, Dagher SM, Sattout EJ (1995) Antifungal activity of the essential oil of Origanum syriacum L. J Food Prot 58:1147\u20131149. https:\/\/doi.org\/10.4315\/0362-028x-58.10.1147","journal-title":"J Food Prot"},{"key":"10053_CR14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/ani11102924","volume":"99","author":"N de Groot","year":"2021","unstructured":"de Groot N, Fari\u00f1as F, Cabrera-G\u00f3mez CG, Pallares FJ, Ramis G (2021) Weaning causes a prolonged but transient change in immune gene expression in the intestine of piglets. J Anim Sci 99:1\u201312. https:\/\/doi.org\/10.3390\/ani11102924","journal-title":"J Anim Sci"},{"key":"10053_CR15","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1046\/j.1365-2672.2000.00969.x","volume":"88","author":"HJD Dorman","year":"2000","unstructured":"Dorman HJD, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 88:308\u2013316. https:\/\/doi.org\/10.1046\/j.1365-2672.2000.00969.x","journal-title":"J Appl Microbiol"},{"key":"10053_CR16","unstructured":"European Commission\u00a0(1998) Council Directive 98\/58\/EC of 20 July 1998 concerning the protection of animals kept for farming purposes. Off J L 221:23\u201327"},{"key":"10053_CR17","unstructured":"European Commission (2008) Council Directive 2008\/120\/EC of 18 December 2008 laying down minimum standards for the protection of pigs. Off J L 47:5\u201313"},{"key":"10053_CR18","doi-asserted-by":"publisher","first-page":"164","DOI":"10.3382\/japr\/pfy059","volume":"28","author":"I Giannenas","year":"2019","unstructured":"Giannenas I, Bonos E, Filliousis G, Stylianaki I, Kumar P, Lazari D, Christaki E, Florou-Paneri P (2019) Effect of a polyherbal or an arsenic-containing feed additive on growth performance of broiler chickens, intestinal microbiota, intestinal morphology, and lipid oxidation of breast and thigh meat. J Appl Poult 28:164\u2013175. https:\/\/doi.org\/10.3382\/japr\/pfy059","journal-title":"J Appl Poult"},{"key":"10053_CR19","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1002\/(SICI)1521-4141(199802)28:02<730:AID-IMMU730>3.0.CO;2-U","volume":"28","author":"D Guy-Grand","year":"1998","unstructured":"Guy-Grand D, DiSanto JP, Henchoz P, Malassis-S\u00e9ris M, Vassalli P (1998) Small bowel enteropathy: role of intraepithelial lymphocytes and of cytokines (IL-12, IFN-\u03b3, TNF) in the induction of epithelial cell death and renewal. Eur J Immunol 28:730\u2013744. https:\/\/doi.org\/10.1002\/(SICI)1521-4141(199802)28:02%3c730:AID-IMMU730%3e3.0.CO;2-U","journal-title":"Eur J Immunol"},{"key":"10053_CR20","doi-asserted-by":"publisher","first-page":"3020","DOI":"10.2527\/1993.71113020x","volume":"71","author":"JD Hahn","year":"1993","unstructured":"Hahn JD, Baker DH (1993) Growth and plasma zinc responses of young pigs fed pharmacologic levels of zinc. J Anim Sci 71:3020\u20133024. https:\/\/doi.org\/10.2527\/1993.71113020x","journal-title":"J Anim Sci"},{"key":"10053_CR21","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1038\/ni1101-997","volume":"2","author":"A Hayday","year":"2001","unstructured":"Hayday A, Theodoridis E, Ramsburg E, Shires J (2001) Intraepithelial lymphocytes: exploring the Third Way in immunology. Nat Immunol 2:997\u20131003. https:\/\/doi.org\/10.1038\/ni1101-997","journal-title":"Nat Immunol"},{"key":"10053_CR22","unstructured":"Hern\u00e1ndez-Coronado AC (2020) Evaluaci\u00f3n del aceite de or\u00e9gano (Lippia berlandieri Schauer) sobre el comportamiento productivo de pollos de engorde. In: Repositorio Acad\u00e9mico Digital. Universidad Aut\u00f3noma de Nuevo Le\u00f3n. http:\/\/eprints.uanl.mx\/19982\/1\/1080314465.pdf. Accesed 16 Jan 2021"},{"key":"10053_CR23","doi-asserted-by":"publisher","first-page":"G956","DOI":"10.1152\/ajpgi.00441.2004","volume":"288","author":"KM Hoque","year":"2005","unstructured":"Hoque KM, Rajendran VM, Binder HJ (2005) Zinc inhibits cAMP-stimulated Cl secretion via basolateral K-channel blockade in rat ileum. Am J Physiol 288:G956\u2013G963. https:\/\/doi.org\/10.1152\/ajpgi.00441.2004","journal-title":"Am J Physiol"},{"key":"10053_CR24","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1017\/s0007114512005508","volume":"110","author":"C Hu","year":"2013","unstructured":"Hu C, Song J, Li Y, Luan Z, Zhu K (2013) Diosmectite\u2013zinc oxide composite improves intestinal barrier function, modulates expression of pro-inflammatory cytokines and tight junction protein in early weaned pigs. Br J Nutr 110:681\u2013688. https:\/\/doi.org\/10.1017\/s0007114512005508","journal-title":"Br J Nutr"},{"key":"10053_CR25","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.anifeedsci.2011.12.022","volume":"173","author":"J Kim","year":"2012","unstructured":"Kim J, Hansen C, Mullan B, Pluske J (2012a) Nutrition and pathology of weaner pigs: Nutritional strategies to support barrier function in the gastrointestinal tract. Anim Feed Sci Technol 173:3\u201316. https:\/\/doi.org\/10.1016\/j.anifeedsci.2011.12.022","journal-title":"Anim Feed Sci Technol"},{"key":"10053_CR26","doi-asserted-by":"publisher","first-page":"165","DOI":"10.4110\/in.2012.12.5.165","volume":"12","author":"SH Kim","year":"2012","unstructured":"Kim SH, Lee KY, Jang YS (2012b) Mucosal immune system and M cell-targeting strategies for oral mucosal vaccination. Immune Netw 12:165\u2013175. https:\/\/doi.org\/10.4110\/in.2012.12.5.165","journal-title":"Immune Netw"},{"key":"10053_CR27","doi-asserted-by":"publisher","first-page":"989","DOI":"10.3390\/molecules22060989","volume":"22","author":"N Leyva-L\u00f3pez","year":"2017","unstructured":"Leyva-L\u00f3pez N, Guti\u00e9rrez-Grijalva EP, Vazquez-Olivo G, Heredia JB (2017) Essential oils of oregano: biological activity beyond their antimicrobial properties. Molecules 22:989. https:\/\/doi.org\/10.3390\/molecules22060989","journal-title":"Molecules"},{"key":"10053_CR28","doi-asserted-by":"publisher","first-page":"22805","DOI":"10.1038\/srep22805","volume":"6","author":"WR Li","year":"2016","unstructured":"Li WR, Shi QS, Dai HQ, Liang Q, Xie XB, Huang XM, Zhao GZ, Zhang L (2016) Antifungal activity, kinetics, and molecular mechanism of action of garlic oil against Candida albicans. Sci Rep 6:22805. https:\/\/doi.org\/10.1038\/srep22805","journal-title":"Sci Rep"},{"key":"10053_CR29","doi-asserted-by":"publisher","first-page":"2050","DOI":"10.2527\/jas.2013-6422","volume":"92","author":"Y Liu","year":"2014","unstructured":"Liu Y, Song M, Che TM, Lee JJ, Bravo D, Maddox CW, Pettigrew JE (2014) Dietary plant extracts modulate gene expression profiles in ileal mucosa of weaned pigs after an Escherichia coli infection. J Anim Sci 92:2050\u20132062. https:\/\/doi.org\/10.2527\/jas.2013-6422","journal-title":"J Anim Sci"},{"key":"10053_CR30","doi-asserted-by":"publisher","first-page":"762","DOI":"10.18805\/ijar.B-836","volume":"53","author":"SD Liu","year":"2019","unstructured":"Liu SD, Song MH, Yun W, Lee JH, Lee CH, Kwak WG, Oh HJ, Kim HB, Cho JH (2019) Effects of oral administration of various essential oils on blood metabolites, intestine development, microbial enumeration, and meat quality in broilers. Indian J Anim Res 53:762\u2013767. https:\/\/doi.org\/10.18805\/ijar.B-836","journal-title":"Indian J Anim Res"},{"key":"10053_CR31","doi-asserted-by":"publisher","first-page":"2924","DOI":"10.3390\/ani11102924","volume":"11","author":"M L\u00f3pez","year":"2021","unstructured":"L\u00f3pez M, Madrid J, Hern\u00e1ndez F, Ros MA, Segura JC, L\u00f3pez MJ, Pallar\u00e9s FJ, S\u00e1nchez CJ, Mart\u00ednez-Mir\u00f3 S (2021) Effect of feed supplementation with Clostridium butyricum, alone or in combination with carob meal or citrus pulp, on digestive and metabolic status of piglets. Animals 11:2924. https:\/\/doi.org\/10.3390\/ani11102924","journal-title":"Animals"},{"key":"10053_CR32","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1080\/17450390903499915","volume":"64","author":"J Michiels","year":"2010","unstructured":"Michiels J, Missotten J, Van Hoorick A, Ovyn A, Fremaut D, De Smet S, Dierick N (2010) Effects of dose and formulation of carvacrol and thymol on bacteria and some functional traits of the gut in piglets after weaning. Arch Anim Nutr 64:136\u2013154. https:\/\/doi.org\/10.1080\/17450390903499915","journal-title":"Arch Anim Nutr"},{"key":"10053_CR33","unstructured":"Miralles A, Otal J, Palacios C, Mart\u00edn P, Le\u00f3n L, Cubero MJ (2014) Evaluaci\u00f3n in vivo de la actividad antibacteriana y promotora del crecimiento de la moltura de ajo (ZooAllium) en pollos ecol\u00f3gicos. Exponed at the XIX Simposio Anual de Asociaci\u00f3n de Veterinarios Especialistas en diagn\u00f3stico laboratorial (AVEDILA), Zaragoza, Spain"},{"key":"10053_CR34","doi-asserted-by":"publisher","unstructured":"Moberg GP, Mench JA (2000) The biology of animal stress: basic principles and implications for animal welfare. Wallingford, Oxfordshire.\u00a0https:\/\/doi.org\/10.1079\/9780851993591.0000","DOI":"10.1079\/9780851993591.0000"},{"key":"10053_CR35","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1076\/phbi.41.6.421.17825","volume":"41","author":"A Pandey","year":"2003","unstructured":"Pandey A, Rai M, Acharya D (2003) Chemical composition and antimycotic activity of the essential oils of corn mint (Mentha arvensis) and lemon grass (Cymbopogon flexuosus) against human pathogenic. Fungi Pharm Biol 41:421\u2013425. https:\/\/doi.org\/10.1076\/phbi.41.6.421.17825","journal-title":"Fungi Pharm Biol"},{"key":"10053_CR36","doi-asserted-by":"publisher","first-page":"e10386","DOI":"10.1371\/journal.pone.0010386","volume":"5","author":"A Patel","year":"2010","unstructured":"Patel A, Mamtani M, Dibley MJ, Badhoniya N, Kulkarni H (2010) Therapeutic value of zinc supplementation in acute and persistent diarrhea: a systematic review. PLoS ONE 5:e10386. https:\/\/doi.org\/10.1371\/journal.pone.0010386","journal-title":"PLoS ONE"},{"key":"10053_CR37","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1093\/jn\/134.3.641","volume":"134","author":"S Pi\u00e9","year":"2004","unstructured":"Pi\u00e9 S, Lall\u00e8s JP, Blazy F, Laffitte J, S\u00e8ve B, Oswald IP (2004) Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets. J Nutr 134:641\u2013647. https:\/\/doi.org\/10.1093\/jn\/134.3.641","journal-title":"J Nutr"},{"key":"10053_CR38","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1016\/0301-6226(95)00039-n","volume":"43","author":"HD Poulsen","year":"1995","unstructured":"Poulsen HD, Larsen T (1995) Zinc excretion and retention in growing pigs fed increasing levels of zinc oxide. Livest Prod Sci 43:235\u2013242. https:\/\/doi.org\/10.1016\/0301-6226(95)00039-n","journal-title":"Livest Prod Sci"},{"key":"10053_CR39","doi-asserted-by":"publisher","first-page":"2093","DOI":"10.3390\/ani10112093","volume":"10","author":"J Rivera-Gomis","year":"2020","unstructured":"Rivera-Gomis J, Peres C, Mart\u00ednez-Conesa C, Otal J, Cer\u00f3n JJ, Escribano D, Cubero MJ (2020) Effects of dietary supple-mentation of garlic and oregano essential oil on biomarkers of oxidative status, stress and inflammation in post-weaning piglets. J Anim Sci 10:2093. https:\/\/doi.org\/10.3390\/ani10112093","journal-title":"J Anim Sci"},{"key":"10053_CR40","first-page":"101","volume":"32","author":"E Rojo","year":"2016","unstructured":"Rojo E, Miralles A, Bernab\u00e9 A, Cubero MJ (2016) Efecto del ajo morado en el rendimiento productivo y en la salud intestinal de pollos de engorde en producci\u00f3n industrial y ecol\u00f3gica. An Vet Mur 32:101\u2013103","journal-title":"An Vet Mur"},{"key":"10053_CR41","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1016\/j.lwt.2004.04.001","volume":"37","author":"KH Sallam","year":"2004","unstructured":"Sallam KH, Ishioroshi IM, Samejima K (2004) Antioxid antimicrob Eff garlic chick sausage. Leb Wiss U Technol 37:849\u2013855. https:\/\/doi.org\/10.1016\/j.lwt.2004.04.001","journal-title":"Leb Wiss U Technol"},{"key":"10053_CR42","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.vetimm.2010.05.001","volume":"137","author":"HR Sargeant","year":"2010","unstructured":"Sargeant HR, McDowall KJ, Miller HM, Shaw MA (2010) Dietary zinc oxide affects the expression of genes associated with inflammation: transcriptome analysis in piglets challenged with ETEC K88. Veter Immunol Immunopathol 137:120\u2013129. https:\/\/doi.org\/10.1016\/j.vetimm.2010.05.001","journal-title":"Veter Immunol Immunopathol"},{"key":"10053_CR43","doi-asserted-by":"publisher","first-page":"2113","DOI":"10.1016\/j.molimm.2011.07.002","volume":"48","author":"HR Sargeant","year":"2011","unstructured":"Sargeant HR, Miller HM, Shaw MA (2011) Inflammatory response of porcine epithelial IPEC J2 cells to enterotoxigenic E. coli infection is modulated by zinc supplementation. Mol Immunol 48:2113\u20132121. https:\/\/doi.org\/10.1016\/j.molimm.2011.07.002","journal-title":"Mol Immunol"},{"key":"10053_CR44","doi-asserted-by":"publisher","first-page":"193","DOI":"10.3109\/02844319009041278","volume":"24","author":"TA S\u00f6derberg","year":"1990","unstructured":"S\u00f6derberg TA, Sunzel B, Holm S, Elmros T, Hallmans G, Sj\u00f6berg S (1990) Antibacterial effect of zinc oxide in vitro. Scand J Plast Reconstr Surg Hand Surg 24:193\u2013197. https:\/\/doi.org\/10.3109\/02844319009041278","journal-title":"Scand J Plast Reconstr Surg Hand Surg"},{"key":"10053_CR45","unstructured":"Standing Committee on Veterinary Medicinal Products (2017) https:\/\/ec.europa.eu\/health\/documents\/community-register\/2017\/20170626136754\/dec_136754_en.pdf. Accessed 11 Nov 2021"},{"key":"10053_CR46","first-page":"63","volume":"15","author":"MR Tatara","year":"2008","unstructured":"Tatara MR, Sliwa E, Dudek K, Gawron A, Piersiak T, Dobrowolski P, Mosiewicz J, Siwicki A, Studzinski T (2008) Aged garlic extract and allicin improve performance and gastrointestinal tract development of piglets reared in artificial sow. Ann Agric Environ Med 15:63\u201369","journal-title":"Ann Agric Environ Med"},{"key":"10053_CR47","doi-asserted-by":"publisher","first-page":"218","DOI":"10.2141\/jpsa.0160071","volume":"54","author":"A Tzora","year":"2016","unstructured":"Tzora A, Giannenas I, Karamoutsios A, Papaioannou N, Papanastasiou D, Bonos E, Skoufos S, Bartzanas T, Skoufos I (2016) Effects of oregano, attapulgite, benzoic acid and their blend on chicken performance, intestinal microbiology, and intestinal morphology. J Poult Sci 54:218\u2013227. https:\/\/doi.org\/10.2141\/jpsa.0160071","journal-title":"J Poult Sci"},{"key":"10053_CR48","doi-asserted-by":"publisher","first-page":"6689","DOI":"10.1128\/AEM.03075-09","volume":"76","author":"W Vahjen","year":"2010","unstructured":"Vahjen W, Pieper R, Zentek J (2010) Bar-coded pyrosequencing of 16S rRNA gene amplicons reveals changes in ileal porcine bacterial communities due to high dietary zinc intake. Appl Environ Microbiol 76:6689\u20136691. https:\/\/doi.org\/10.1128\/AEM.03075-09","journal-title":"Appl Environ Microbiol"},{"key":"10053_CR49","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1080\/0003942032000161054","volume":"57","author":"CJ Van Nevel","year":"2003","unstructured":"Van Nevel CJ, Decuypere JA, Dierick N, Molly K (2003) The influence of Lentinus edodes (Shiitake mushroom) preparations on bacteriological and morphological aspects of the small intestine in piglets. Arch Tierernahr 57:399\u2013412. https:\/\/doi.org\/10.1080\/0003942032000161054","journal-title":"Arch Tierernahr"},{"key":"10053_CR50","first-page":"14","volume":"2","author":"R Vega Montalvo","year":"1997","unstructured":"Vega Montalvo R, Carrillo Dom\u00ednguez C (1997) Efecto sobre la motilidad intestinal y toxicidad aguda oral del extracto fluido de Ocimum gratissimum L. (or\u00e9gano cimarr\u00f3n). Rev Cuba Plantas Med 2:14\u201318","journal-title":"Rev Cuba Plantas Med"},{"key":"10053_CR51","doi-asserted-by":"publisher","first-page":"2123","DOI":"10.2527\/jas.2010-3186","volume":"89","author":"JP Wang","year":"2011","unstructured":"Wang JP, Yoo JS, Jang HD, Lee JH, Cho JH, Kim IH (2011) Effect of dietary fermented garlic by Weissella koreensis powder on growth performance, blood characteristics, and immune response of growing pigs challenged with Escherichia coli lipopoly-saccharide. J Anim Sci 89:2123\u20132131. https:\/\/doi.org\/10.2527\/jas.2010-3186","journal-title":"J Anim Sci"},{"key":"10053_CR52","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1017\/s0007114510005660","volume":"105","author":"PJA Wijtten","year":"2011","unstructured":"Wijtten PJA, Van Der Meulen J, Verstegen MWA (2011) Intestinal barrier function and absorption in pigs after weaning: A review. Br J Nutr 105:967\u2013981. https:\/\/doi.org\/10.1017\/s0007114510005660","journal-title":"Br J Nutr"},{"key":"10053_CR53","doi-asserted-by":"publisher","first-page":"1590","DOI":"10.13702\/j.1000-0607.190051","volume":"28","author":"TP Zhao","year":"2010","unstructured":"Zhao TP, Zhou S, Qin SD (2010) The regulating effect on different moxibustion for colonic mucosa mucin in rats with ulcerative colitis. Chinese Arc Tradit Chinese Med 28:1590. https:\/\/doi.org\/10.13702\/j.1000-0607.190051","journal-title":"Chinese Arc Tradit Chinese Med"},{"key":"10053_CR54","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1111\/j.1751-2980.2011.00569.x","volume":"13","author":"H Zhu","year":"2012","unstructured":"Zhu H, Jia Z, Misra H, Li YR (2012) Oxidative stress and redox signalling mechanisms of alcoholic liver disease: Updated experimental and clinical evidence. J Dig Dis 13:133\u2013142. https:\/\/doi.org\/10.1111\/j.1751-2980.2011.00569.x","journal-title":"J Dig Dis"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-022-10053-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-022-10053-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-022-10053-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T10:34:27Z","timestamp":1684924467000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11259-022-10053-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,15]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10053"],"URL":"https:\/\/doi.org\/10.1007\/s11259-022-10053-2","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,15]]},"assertion":[{"value":"20 May 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 December 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 December 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The University of Murcia, through the Ethical Committee for Animal Experimentation (CEEA), approved the experimental protocols used in the study (Authorization Code 471\/2018).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and consent to participate"}},{"value":"All contributing authors have been asked to give consent to publication at the point of acceptance, to confirm that they have approved the final version of the manuscript and have made all required statements and declarations.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors have read the journal\u2019s policy and have the following competing interests: the co-author (J.A.T) is employee of Dalland Hybrid Espa\u00f1a S.A. The other authors have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}