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Diagnosis of <jats:italic>S. vulgaris<\/jats:italic> infections can be determined with next generation sequencing techniques, which are inherently dependent on reference sequences. The best marker for parasitic nematodes is internal transcribed spacer 2 (ITS2) and we provide the first complete ITS2 sequences from five morphologically identified <jats:italic>S. vulgaris<\/jats:italic> and additional sequences from two <jats:italic>S. edentatus<\/jats:italic>. These sequences have high similarity to already published partial sequences and amplicon sequence variants (ASV) based on next generation sequencing (NGS). The ITS2 sequences from <jats:italic>S. vulgaris<\/jats:italic> matched available partial ITS2 sequences and the full ASVs, whereas the <jats:italic>S. edentatus<\/jats:italic> sequence matched another complete sequence. We also compare Sanger sequencing and NGS methods and conclude that the ITS2 variation is better represented with NGS methods. Based on this, we recommend that further sequencing of morphologically identified specimens of various species should be performed with NGS cover the intraspecific variation in the ITS2.<\/jats:p>","DOI":"10.1007\/s11259-022-10067-w","type":"journal-article","created":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T12:04:07Z","timestamp":1672833847000},"page":"1767-1771","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The complete ITS2 barcoding region for Strongylus vulgaris and Strongylus edentatus"],"prefix":"10.1007","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6940-5634","authenticated-orcid":false,"given":"Peter","family":"Halvarsson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Tyd\u00e9n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,1,4]]},"reference":[{"key":"10067_CR1","doi-asserted-by":"publisher","first-page":"e0143559","DOI":"10.1371\/journal.pone.0143559","volume":"10","author":"RW Avramenko","year":"2015","unstructured":"Avramenko RW, Redman EM, Lewis R et al (2015) Exploring the gastrointestinal \u201cNemabiome\u201d: deep amplicon sequencing to quantify the species composition of parasitic nematode communities. 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