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After oral ingestion and passage through the digestive tract of animals, this microorganism captures the nematodes in the feces. The drastic conditions of ruminant digestive tract could affect fungi chlamydospores and therefore biocontrol activity. The aim of this study was to evaluate in vitro the effect of four ruminant digestive segments on the concentration and nematode predatory ability of a Colombian native strain of <jats:italic>D. flagrans<\/jats:italic>. The sequential four-step methodology proposed evaluated conditions of the oral cavity, rumen, abomasum, and small intestine such as pH (2, 6, 8), enzymes (pepsin, pancreatin), temperature (39\u00a0\u00b0C), and anaerobiosis comparing short (7\u00a0h) and long (51\u00a0h) exposure times. The results showed that the nematode predatory ability of the fungi is affected by sequential exposure to gastrointestinal segments and this effect depends on the exposure time to those conditions. After short exposure (7\u00a0h) through the four ruminant digestive segments, the fungi had a nematode predatory ability of 62%, in contrast, after long exposure (51\u00a0h) the nematode predatory ability was lost (0%). Moreover, the number of broken chlamydospores was higher in the long-exposure assay.\n<\/jats:p>","DOI":"10.1007\/s11259-023-10089-y","type":"journal-article","created":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T10:01:28Z","timestamp":1680516088000},"page":"1405-1412","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Nematode predatory ability of the fungus Duddingtonia flagrans affected by in vitro sequential exposure to ovine gastrointestinal tract"],"prefix":"10.1007","volume":"47","author":[{"given":"Elizabeth","family":"C\u00e9spedes-Guti\u00e9rrez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Marcela","family":"Arag\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha Isabel","family":"G\u00f3mez-\u00c1lvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime Andr\u00e9s","family":"Cubides-C\u00e1rdenas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego Francisco","family":"Cort\u00e9s-Rojas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,4,3]]},"reference":[{"key":"10089_CR1","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1079\/bjn19700104","volume":"24","author":"JA Bines","year":"1970","unstructured":"Bines JA, Davey AWF (1970) Voluntary intake, digestion, rate of passage, amount of material in the alimentary tract and behaviour in cows receiving complete diets containing straw and concentrates in different proportions. 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