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However, there are few information on their characteristics. Our aim was to evaluate A-72 expression level of genes and proteins involved in the innate immune response and cell cycle, their ability to respond to infective stressors and their possible use as a cellular model for anti-cancer studies in human and animal medicine. For this purpose, we evaluated the basal expression of immune-related, cell cycle and DNA repair genes on this cell line and tumoral tissues. A-72 ability to respond to a wild-type strain of <jats:italic>Salmonella typhimurium<\/jats:italic> was assessed. <jats:italic>S. typhimurium<\/jats:italic> showed ability to penetrate A-72 causing pro-inflammatory response accompanied by a decrease of cell viability. <jats:italic>IL10<\/jats:italic> and <jats:italic>IL18<\/jats:italic> genes were not expressed in A-72 while <jats:italic>CXCL8<\/jats:italic>, <jats:italic>NOS2<\/jats:italic>, <jats:italic>CXCR4<\/jats:italic> and <jats:italic>PTEN<\/jats:italic> were highly expressed in all samples and <jats:italic>TP53<\/jats:italic> was slightly expressed, as shown in human STS. Our results outline the ability of A-72 to respond to a bacterial agent by modifying the expression of important genes involved in innate immune response and provide a useful model for in vitro evaluation of new therapeutic approaches that could be translated into the human oncology.<\/jats:p>","DOI":"10.1007\/s11259-023-10115-z","type":"journal-article","created":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T01:02:37Z","timestamp":1681174957000},"page":"1615-1627","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line"],"prefix":"10.1007","volume":"47","author":[{"given":"Elisabetta","family":"Razzuoli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Chirullo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara Grazia","family":"De Ciucis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samanta","family":"Mecocci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabella","family":"Martini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Zoccola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Campanella","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katia","family":"Varello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Petrucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Di Meo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Bozzetta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Tarantino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Goria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Modesto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,4,11]]},"reference":[{"key":"10115_CR1","doi-asserted-by":"crossref","unstructured":"Amadori & Razzuoli (2014) Immune Control of PRRS: Lessons to be Learned and Possible Ways Forward - PubMed. https:\/\/pubmed.ncbi.nlm.nih.gov\/26664910\/. 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