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Thus, novel therapeutic approaches are needed. Synthetic analogues of curcumin have elicited potent anti-cancer activity in multiple in vitro and in vivo models of human cancer. Furthermore, the compound 3,5-bis(3,4,5-trimethoxybenzylidene)-1-methylpiperidine-4-one (RL71) has recently exhibited potent cell cycle arrest and apoptotic induction in a canine osteosarcoma cell line. To determine its potency in canine histiocytic sarcoma cells, cell viability of DH82 and Nike cells was measured using the sulforhodamine B assay. Flow cytometry was then used to analyse both cell cycle distribution and apoptosis. Of the five curcumin analogues examined, RL71, had the highest potency with EC\n                    <jats:sub>50<\/jats:sub>\n                    values of 0.66 \u00b1 0.057 \u00b5M and 0.79 \u00b1 0.13 \u00b5M in the DH82 and Nike cell lines, respectively. Furthermore, RL71 at the 1x EC\n                    <jats:sub>50<\/jats:sub>\n                    concentration increased G2\/M cell cycle arrest 2-fold, and at the 2x EC\n                    <jats:sub>50<\/jats:sub>\n                    concentration increased the number of apoptotic cells 4-fold. These findings are consistent with previous work using RL71 in both canine and human cancer cell lines. Future research should be directed on time-dependent changes, and mechanistic investigation in greater detail to elucidate RL71 mechanisms of action.\n                  <\/jats:p>","DOI":"10.1007\/s11259-023-10201-2","type":"journal-article","created":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T01:01:30Z","timestamp":1692406890000},"page":"563-568","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Utility of the second-generation curcumin analogue RL71 in canine histiocytic sarcoma"],"prefix":"10.1007","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5766-0620","authenticated-orcid":false,"given":"Barnaby","family":"Kelly","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8914-7767","authenticated-orcid":false,"given":"Douglas","family":"Thamm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9718-0719","authenticated-orcid":false,"given":"Rhonda J.","family":"Rosengren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,8,19]]},"reference":[{"key":"10201_CR1","doi-asserted-by":"publisher","first-page":"S19","DOI":"10.1093\/jhered\/esp039","volume":"100","author":"J Abadie","year":"2009","unstructured":"Abadie J, H\u00e9dan B, Cadieu E, De Brito C, Devauchelle P, Bourgain C, Parker HG, Vaysse A, Margaritte-Jeannin P, Galibert F, Ostrander EA, Andr\u00e9 C (2009) Epidemiology, Pathology, and Genetics of Histiocytic Sarcoma in the Bernese Mountain Dog Breed. 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