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These infections are the most important tick-borne zoonotic diseases involving ticks as vectors. Descriptive and epidemiological studies are essential to determine the animal hosts involved in the maintenance of these diseases. In the present study, 94 tick pool samples from 15 different host species located in the Region of Murcia (southeastern, Spain) were analysed. Ticks were morphologically identified as: <jats:italic>Dermacentor marginatus, Hyalomma lusitanicum, Ixodes Ricinus<\/jats:italic>, and <jats:italic>Rhipicephalus sanguineus<\/jats:italic>. Our results showed that 5.3% of the tick pool samples carried <jats:italic>Borrelia<\/jats:italic> spp. DNA, and 20.2% carried SFG <jats:italic>Rickettsia<\/jats:italic> DNA. In every hard tick pool Spot Fever Group (SFG) <jats:italic>Rickettsia<\/jats:italic> spp. DNA were detected, except for <jats:italic>H. lusitanicum.<\/jats:italic> Likewise, <jats:italic>D. marginatum<\/jats:italic> was the only species in which <jats:italic>Borrelia<\/jats:italic> spp. DNA was not detected. Barbary sheep and wild boar were the host species in which tick pools showed DNA presence of both pathogens. This study increases the knowledge about the presence of <jats:italic>Borrelia<\/jats:italic> spp. DNA and SFG <jats:italic>Rickettsia<\/jats:italic> spp. DNA in different hard tick species from this geographical area.<\/jats:p>","DOI":"10.1007\/s11259-023-10292-x","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T05:03:10Z","timestamp":1705467790000},"page":"1785-1790","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular identification of Borrelia and SFG Rickettsia spp. in hard ticks parasitizing domestic and wild animals in southeastern Spain"],"prefix":"10.1007","volume":"48","author":[{"given":"N.","family":"Ortega","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Arcenillas-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MI","family":"Villa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MD","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MR","family":"Caro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,1,17]]},"reference":[{"key":"10292_CR1","doi-asserted-by":"crossref","unstructured":"Akl T, Bourgoin G, Souq ML, Appolinaire J, Poirel MT, Gibert P, Rizk GA, Garel M, Zenner L (2019) Detection of tick-borne pathogens in questing Ixodes ricinus in the French Pyrenees and first identification of Rickettsia monacensis in France, vol 26. 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