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Samples from lung, liver, spleen, kidney, tongue and intestine were investigated by PCRs targeting the following genes: <jats:italic>tet<\/jats:italic>(A), <jats:italic>tet<\/jats:italic>(B), <jats:italic>tet<\/jats:italic>(C), <jats:italic>tet<\/jats:italic>(D), <jats:italic>tet<\/jats:italic>(E), <jats:italic>tet<\/jats:italic>(G), <jats:italic>tet<\/jats:italic>(K), <jats:italic>tet<\/jats:italic>(L), <jats:italic>tet<\/jats:italic>(M), <jats:italic>tet<\/jats:italic>(O), <jats:italic>tetA<\/jats:italic>(P), <jats:italic>tet<\/jats:italic>(Q), <jats:italic>tet<\/jats:italic>(S), <jats:italic>tet<\/jats:italic>(X), <jats:italic>sul1<\/jats:italic>, <jats:italic>sul2<\/jats:italic>, <jats:italic>sul3<\/jats:italic>, <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2212M<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub> and <jats:italic>mcr<\/jats:italic>-1. A PCR positivity was highlighted for 13 out of the 21 tested genes; no positive results were obtained for <jats:italic>tet<\/jats:italic>(C), <jats:italic>tet<\/jats:italic>(D), <jats:italic>tet<\/jats:italic>(E), <jats:italic>tet<\/jats:italic>(G), <jats:italic>sul3<\/jats:italic>, <jats:italic>bla<\/jats:italic><jats:sub>CTX<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub> and <jats:italic>mcr<\/jats:italic>-1 genes. All 11 animals sampled showed positivity for one or more resistance genes. The results confirm the potential role of the wolf as an indicator and\/or vector of antimicrobial-resistant bacteria or ARGs.<\/jats:p>","DOI":"10.1007\/s11259-024-10354-8","type":"journal-article","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T00:05:24Z","timestamp":1710806724000},"page":"1941-1947","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Antimicrobial resistance in wildlife: detection of antimicrobial resistance genes in Apennine wolves (Canis lupus italicus Altobello, 1921) from Central Italy"],"prefix":"10.1007","volume":"48","author":[{"given":"A.","family":"Di Francesco","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Salvatore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Ranucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Gobbi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Morandi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,3,19]]},"reference":[{"key":"10354_CR1","doi-asserted-by":"publisher","first-page":"20160137","DOI":"10.1098\/rsbl.2016.0137","volume":"12","author":"KE Arnold","year":"2016","unstructured":"Arnold KE, Williams NJ, Bennett M (2016) Disperse abroad in the land\u2019: the role of wildlife in the dissemination of antimicrobial resistance. 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