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They have a considerably wide range of final host, including many species of family Cervidae. Intermediate hosts and vectors at the same time, are represented by the several mosquito species, mostly of genus <jats:italic>Aedes.<\/jats:italic> Infection of <jats:italic>Setaria<\/jats:italic> is relatively harmless and especially in wild cervids usually pass unnoticed. Although in some cases it can induce peritonitis which might be a life threatening condition.<\/jats:p><jats:p>This study was determined to reveal the presence of helminths <jats:italic>Setaria tundra<\/jats:italic> and <jats:italic>Setaria cervi<\/jats:italic> in red deer (<jats:italic>Cervus elaphus<\/jats:italic>) in Slovakia. The parasites were identified morphologically and genetically, based on the sequences of a fragment of the mitochondrial cytochrome c oxidase subunit 1 (<jats:italic>cox<\/jats:italic>1) gene. For this purpose we used partial results of our longer parasitological monitoring realized in one particular hunting area located in eastern Slovakia, near the city of Ko\u0161ice. A total of 60 red deer individuals were tested, of which one was found to be infected with <jats:italic>Setaria tundra<\/jats:italic> (prevalence of 1.7%) and four were detected to be infected with <jats:italic>Setaria cervi<\/jats:italic> (prevalence 6.7%). The intensity of infection was very low, only one specimen of <jats:italic>Setaria<\/jats:italic> spp. in each positive animal.<\/jats:p>","DOI":"10.1007\/s11259-024-10394-0","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T08:02:12Z","timestamp":1718352132000},"page":"2835-2840","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular detection of Setaria tundra (Issaitshikoff &amp; Rajewskaya, 1928) and Setaria cervi (Rudolphi, 1819) in red deer in Slovakia"],"prefix":"10.1007","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5596-4147","authenticated-orcid":false,"given":"Jozef","family":"Laz\u00e1r","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4730-2386","authenticated-orcid":false,"given":"J\u00falia","family":"\u0160migov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3881-8524","authenticated-orcid":false,"given":"\u013dubom\u00edr","family":"\u0160miga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3952-4787","authenticated-orcid":false,"given":"Federica","family":"Berrilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Laz\u00e1r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7336-9242","authenticated-orcid":false,"given":"J\u00e1n","family":"\u010curl\u00edk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2701-6905","authenticated-orcid":false,"given":"Ingrid","family":"Papajov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,14]]},"reference":[{"key":"10394_CR1","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1007\/s00436-011-2486-1","volume":"110","author":"S Alasaad","year":"2012","unstructured":"Alasaad S, Pascucci I, Jowers MJ, Soriguer RC, Zhu XQ, Rossi L (2012) Phylogenetic study of Setaria Cervi based on mitochondrial cox 1 gene sequences. 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