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This requirement is also supported by the relatively high incidence of dermatological diseases in horses and thus the need to manage them therapeutically. The taxonomic analysis of skin samples (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200930) from five different body parts of clinically healthy Shetlands ponies females (neck, back, abdomen, pastern, muzzle) kept under homogeneous conditions (in open stalls with paddock, feed with dry hay, green grass ad libitum and granulated feed) was performed using amplification of V3\u2014V4 region of the 16S rRNA gene. Results indicate that bacteria associated with healthy equine skin represent 18 phyla, 29 classes and 119 families. The most abundant phyla were Proteobacteria (30.8\u2009\u00b1\u20099.1%) followed by Actinobacteriota (20.4\u2009\u00b1\u20097.6%), Firmicutes (19.5\u2009\u00b1\u200910.1%), Bacteroidota (8.5\u2009\u00b1\u20095.0%) and Deinococcota (7.2\u2009\u00b1\u200914.8%). Among 229 genera identified,\n                    <jats:italic>Corynebacterium<\/jats:italic>\n                    (7.4\u2009\u00b1\u20096.5%) was the most abundant genus in skin sites of horses, followed by\n                    <jats:italic>Deinococcus<\/jats:italic>\n                    (7.1\u2009\u00b1\u200914.9%) and\n                    <jats:italic>Macrococcus<\/jats:italic>\n                    (5.0\u2009\u00b1\u20098.2%). Indices for the richness and diversity of species within bacterial populations for five regions of horses skin revealed no significant variations observed for species richness (Chao1,\n                    <jats:italic>p<\/jats:italic>\n                    -value 0.2001) but significant result for species evenness (Shannon,\n                    <jats:italic>p<\/jats:italic>\n                    -value 0.0049) with maximum on the neck and minimum on the back skin site. The clustering was seen across samples from different skin sites but also across samples collected from individual horses.\n                  <\/jats:p>","DOI":"10.1007\/s11259-024-10444-7","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T00:01:59Z","timestamp":1718841719000},"page":"2847-2855","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Composition and diversity of 16S rRNA based skin bacterial microbiome in healthy horses"],"prefix":"10.1007","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8486-6570","authenticated-orcid":false,"given":"Viola","family":"Strompfov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"\u0160tempelov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,20]]},"reference":[{"key":"10444_CR1","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1016\/S0966-842X(99)01566-8","volume":"7","author":"JR Battista","year":"1999","unstructured":"Battista JR, Earl AM, Park MJ (1999) Why is Deinococcus radiodurans so resistant to ionizing radiation? 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All manipulation was in accordance with standard veterinary practices according Slovak legislation (no. 377\/2012 and 436\/2012).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Illumina MiSeq sequencing data are avaliable in BioProject SRA database uder accesion number PRJNA1113871.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Accession number"}}]}}