{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T15:27:13Z","timestamp":1775575633993,"version":"3.50.1"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100016003","name":"Istanbul University Cerrahpa\u015fa","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100016003","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Obesity stands out as the most common multifactorial nutritional problem affecting domestic cats. According to studies, the prevalence of overweight or obese cats varies between 11.5% and 63%. Various factors such as breed, age, gender, reproductive status, owner-pet relationship, diet type, and environmental factors have been identified as potential risk factors for the development of obesity in cats. Among the genes involved in regulating energy balance, one of the prominent genes is melanocortin-4 receptor gene (<jats:italic>MC4R<\/jats:italic>). A specific missense variant in the feline <jats:italic>MC4R<\/jats:italic> gene (c.92\u00a0C\u2009&gt;\u2009T) has been associated with overweight in diabetic domestic shorthaired cats. In this study, it was aimed to determine the polymorphisms in <jats:italic>MC4R<\/jats:italic> gene in random bred cats and cats belonging to a registered breed in Turkey and to investigate their relationship with obesity. Blood samples from 30 obese and 20 non-obese cats were collected into sterile vacuum EDTA tubes. Exon 1 of the <jats:italic>MC4R<\/jats:italic> was amplified and sequenced. As a result of DNA sequence analysis, we identified a total of six SNPs in the feline <jats:italic>MC4R<\/jats:italic> gene, four of which were found for the first time in this study. As a result of comparing allele frequencies in obese and non-obese cats, a significant relationship was found between SNP rs783632116 and obesity. The results of regression analyses evaluating the effects of SNP genotypes, sex and infertility status on feline Body Mass Index (fBMI) indicated that non-synonymous SNPs rs783632116, ss11356259660 and ss11356259661 were significantly associated with fBMI.<\/jats:p>","DOI":"10.1007\/s11259-025-10700-4","type":"journal-article","created":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T09:55:16Z","timestamp":1741082116000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Missense variants in MC4R gene are associated with obesity in cats"],"prefix":"10.1007","volume":"49","author":[{"given":"Salah Aldin","family":"Mousa Basha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7330-103X","authenticated-orcid":false,"given":"Iraz","family":"Akis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,3,4]]},"reference":[{"issue":"4","key":"10700_CR1","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1016\/j.jcpa.2017.03.006","volume":"156","author":"M Chandler","year":"2017","unstructured":"Chandler M, Cunningham S, Lund EM, Khanna C, Naramore R, Patel A, Day MJ (2017) Obesity and associated comorbidities in people and companion animals: A one health perspective. J Comp Pathol 156(4):296\u2013309. https:\/\/doi.org\/10.1016\/j.jcpa.2017.03.006","journal-title":"J Comp Pathol"},{"issue":"7","key":"10700_CR2","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1016\/j.acthis.2011.12.008","volume":"114","author":"C Dall\u2019Aglio","year":"2012","unstructured":"Dall\u2019Aglio C, Polisca A, Boiti C, Ceccarelli P (2012) Immunolocalization of leptin and its receptor in the placenta of cats. Acta Histochem 114(7):719\u2013722. https:\/\/doi.org\/10.1016\/j.acthis.2011.12.008","journal-title":"Acta Histochem"},{"issue":"7","key":"10700_CR3","doi-asserted-by":"publisher","first-page":"166144","DOI":"10.1016\/j.bbadis.2021.166144","volume":"1867","author":"S Farooq","year":"2021","unstructured":"Farooq S, Rana S, Siddiqui AJ, Iqbal A, Musharraf SG (2021) Association of metabolites with obesity based on two gene variants, MC4R rs17782313 and BDNF rs6265. Biochim Biophys Acta Mol Basis Dis 1867(7):166144. https:\/\/doi.org\/10.1016\/j.bbadis.2021.166144","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"issue":"2","key":"10700_CR4","doi-asserted-by":"publisher","first-page":"175","DOI":"10.3758\/bf03193146","volume":"39","author":"F Faul","year":"2007","unstructured":"Faul F, Erdfelder E, Lang AG, Buchner A (2007) G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods 39(2):175\u2013191. https:\/\/doi.org\/10.3758\/bf03193146","journal-title":"Behav Res Methods"},{"issue":"2","key":"10700_CR5","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1111\/jvim.12275","volume":"28","author":"Y Forcada","year":"2014","unstructured":"Forcada Y, Holder A, Church DB, Catchpole B (2014) A polymorphism in the melanocortin 4 receptor gene (MC4R:c.92c\u2009>\u2009t) is associated with diabetes mellitus in overweight domestic shorthaired cats. 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