{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T04:02:08Z","timestamp":1746763328898,"version":"3.40.5"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003339","name":"Consejo Superior de Investigaciones Cientificas","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003339","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Bats (order Chiroptera) are known as important hosts and reservoirs for several zoonotic viruses. To this date, most virology studies in bats have focused on RNA viruses; consequently, information about DNA viruses is more limited. Herein we surveyed the presence of herpesviruses in blood or spleen samples of three bat species of Spain (n\u2009=\u200931) and five bat species of Chile (n\u2009=\u200950) by using a broad-spectrum nested PCR. Overall, herpesvirus DNA was detected in 9.7% (3\/31) bats of Spain and 10.0% (5\/50) bats of Chile. Three gammaherpesvirus sequence types were found in bats from Spain, while sequence types of two betaherpesviruses, two gammaherpesviruses and one unclassified herpesvirus were detected in Chilean bats, two of which could represent novel herpesvirus species. The impact (if any) of these herpesviruses on the health of the studied species needs to be clarified. This study increases our knowledge of herpesvirus diversity in bats and expands their geographic range in South America. Future herpesvirus surveillance studies are warranted to test chiropteran families other than Vespertilionidae and Molossidae in Chile and Spain.<\/jats:p>","DOI":"10.1007\/s11259-025-10747-3","type":"journal-article","created":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T05:06:54Z","timestamp":1746076014000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular survey of herpesviruses in bats from Chile and Spain reveals potentially novel species"],"prefix":"10.1007","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6111-6301","authenticated-orcid":false,"given":"Carlos","family":"Sacrist\u00e1n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8810-5071","authenticated-orcid":false,"given":"Fernando","family":"Esper\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4169-4884","authenticated-orcid":false,"given":"Irene","family":"Sacrist\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3847-8355","authenticated-orcid":false,"given":"Jordi","family":"Serra Cobo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8204-0895","authenticated-orcid":false,"given":"Marc","family":"L\u00f3pez Roig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1481-3750","authenticated-orcid":false,"given":"Fulgencio","family":"Lis\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5608-781X","authenticated-orcid":false,"given":"Javier","family":"Mill\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,5,1]]},"reference":[{"key":"10747_CR1","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1146\/annurev-virology-092917-043227","volume":"5","author":"W Azab","year":"2018","unstructured":"Azab W, Dayaram A, Greenwood AD, Osterrieder N (2018) How Host Specific Are Herpesviruses? Lessons from Herpesviruses Infecting Wild and Endangered Mammals. Annu Rev Virol 5:53\u201368","journal-title":"Annu Rev Virol"},{"key":"10747_CR2","doi-asserted-by":"publisher","unstructured":"Cistrone L, Russo D. Aulagnier S (2023) Miniopterus schreibersii. The IUCN Red List of Threatened Species, p e.T230918147A230918550. https:\/\/doi.org\/10.2305\/IUCN.UK.2023-1.RLTS.T230918147A230918550.en. Accessed 2 Jan 2025","DOI":"10.2305\/IUCN.UK.2023-1.RLTS.T230918147A230918550.en"},{"key":"10747_CR3","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1038\/s41579-018-0118-9","volume":"17","author":"J Cui","year":"2019","unstructured":"Cui J, Li F, Shi ZL (2019) Origin and evolution of pathogenic coronaviruses. Nat Rev Microbiol 17:181\u2013192","journal-title":"Nat Rev Microbiol"},{"key":"10747_CR4","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.vetmic.2010.02.014","volume":"143","author":"AJ Davison","year":"2010","unstructured":"Davison AJ (2010) Herpesvirus systematics. Vet Microbiol 143:52\u201369","journal-title":"Vet Microbiol"},{"key":"10747_CR5","unstructured":"DBatVir (2024) Batabase of bat-associated virueses. http:\/\/www.mgc.ac.cn\/DBatVir\/. Accessed 7 Feb 2025"},{"key":"10747_CR6","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1111\/brv.12893","volume":"98","author":"F Festa","year":"2023","unstructured":"Festa F, Ancillotto L, Santini L, Pacifici M, Rocha R, Toshkova N, Amorim F, Ben\u00edtez-L\u00f3pez A et al (2023) Bat responses to climate change: a systematic review. Biol Rev Camb Philos Soc 98:19\u201333","journal-title":"Biol Rev Camb Philos Soc"},{"key":"10747_CR7","doi-asserted-by":"publisher","first-page":"001673","DOI":"10.1099\/jgv.0.001673","volume":"102","author":"D Gatherer","year":"2021","unstructured":"Gatherer D, Depledge DP, Hartley CA, Szpara ML, Vaz PK, Benk\u0151 M, Brandt CR, Bryant NA, Dastjerdi A et al (2021) ICTV virus taxonomy profile: herpesviridae 2021. J Gen Virol 102:001673","journal-title":"J Gen Virol"},{"key":"10747_CR8","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1146\/annurev-virology-110615-042203","volume":"3","author":"DT Hayman","year":"2016","unstructured":"Hayman DT (2016) Bats as viral reservoirs. Annu Rev Virol 3:77\u201399","journal-title":"Annu Rev Virol"},{"key":"10747_CR9","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1007\/s00239-019-09913-4","volume":"88","author":"GL Kaj\u00e1n","year":"2020","unstructured":"Kaj\u00e1n GL, Doszpoly A, Tarj\u00e1n ZL, Vidovszky MZ, Papp T (2020) Virus-host coevolution with a focus on animal and human DNA viruses. J Mol Evol 88:41\u201356","journal-title":"J Mol Evol"},{"key":"10747_CR10","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1038\/s41579-020-0394-z","volume":"18","author":"M Letko","year":"2020","unstructured":"Letko M, Seifert SN, Olival KJ, Plowright RK, Munster VJ (2020) Bat-borne virus diversity, spillover and emergence. 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Version 1.7. www.batnames.org. Accessed 03\/21\/2025"},{"key":"10747_CR18","doi-asserted-by":"publisher","first-page":"1666","DOI":"10.1128\/jcm.34.7.1666-1671.1996","volume":"34","author":"DR VanDevanter","year":"1996","unstructured":"VanDevanter DR, Warrener P, Bennett L, Schultz ER, Coulter S, Garber RL, Rose TM (1996) Detection and analysis of diverse herpesviral species by consensus primer PCR. J Clin Microbiol 34:1666\u20131671","journal-title":"J Clin Microbiol"},{"key":"10747_CR19","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/s00705-015-2614-9","volume":"161","author":"XY Zheng","year":"2016","unstructured":"Zheng XY, Qiu M, Chen SW, Xiao JP, Ma LZ, Liu S, Zhou JH, Zhang QH, Li X et al (2016) High prevalence and diversity of viruses of the subfamily Gammaherpesvirinae, family Herpesviridae, in fecal specimens from bats of different species in southern China. 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Nucleic Acids Res 50:D943\u2013D949","journal-title":"Nucleic Acids Res"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-025-10747-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-025-10747-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-025-10747-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T08:21:10Z","timestamp":1746692470000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11259-025-10747-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,1]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10747"],"URL":"https:\/\/doi.org\/10.1007\/s11259-025-10747-3","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"type":"print","value":"0165-7380"},{"type":"electronic","value":"1573-7446"}],"subject":[],"published":{"date-parts":[[2025,5,1]]},"assertion":[{"value":"9 February 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 April 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 May 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Bat captures and sampling in Spain were performed with the permission of the Spanish Regional Committee for Scientific Capture (Permit number 2013: SF\/555), and were authorized by the Bioethics Committee of the University of Barcelona, in compliance with the ethical and integrity standards required by this committee, in accordance with the regulations established by the Catalan and Spanish governments, as well as the European Union. Bat captures and sampling in Chile were authorized by the Servicio Agr\u00edcola y Ganadero (SAG-Chile. Resoluci\u00f3n exenta N\u00ba8431\/2021) of the Chilean Government. No additional ethical authorization was required in Chile at that time.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Animal ethics"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"Bat captures and sampling in Spain and Chile were performed with the permission of the Spanish Regional Committee for Scientific Capture (Permit number 2013: SF\/555) and the Servicio Agr\u00edcola y Ganadero (SAG-Chile. Resoluci\u00f3n exenta N\u00ba8431\/2021) of the Chilean Government, respectively.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Permits"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"185"}}