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As\n                    <jats:italic>M. leachii<\/jats:italic>\n                    has veterinary implications and is related to other foreign animal diseases of economic importance, we sought to investigate differences in adherence to and invasion of different bovine cells between\n                    <jats:italic>M. leachii<\/jats:italic>\n                    type strain PG50 and\n                    <jats:italic>M. bovis<\/jats:italic>\n                    strains (type strain PG45 and field strain 428E). Specifically, we tested the strains with Madin-Darby bovine kidney (MDBK), bovine turbinate (BTu), and the carticular progenitor 5 (CP5) cells for 2 and 24\u00a0h. Additionally, we tested the MDBK and BTu cells with both non-cytopathic bovine viral diarrhea virus (BVDV-1b) positive and BVDV negative fetal bovine serum.\n                    <jats:italic>Mycoplasma leachii<\/jats:italic>\n                    strain PG50 adhered and invaded less efficiently than\n                    <jats:italic>M. bovis<\/jats:italic>\n                    strains 428E and PG45 with a log\n                    <jats:sub>10<\/jats:sub>\n                    reduction in adherence and invasion ranging from 1.5 to 2.9 CFU\/mL. The reduced adherence and invasion of\n                    <jats:italic>M. leachii<\/jats:italic>\n                    type strain PG50 was observed regardless of the incubation time (2\u00a0h vs. 24\u00a0h), tested cells (MDBK, BTu, or CP5), cell type (epithelial or fibroblast), or presence of BVDV. Future work should further characterize\n                    <jats:italic>M. leachii<\/jats:italic>\n                    to further assess its risk to animal health and compare it phenotypically to other members of the\n                    <jats:italic>M. mycoides<\/jats:italic>\n                    cluster.\n                  <\/jats:p>","DOI":"10.1007\/s11259-025-10901-x","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T07:50:24Z","timestamp":1759218624000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mycoplasma leachii strain PG50 shows reduced adherence and invasion compared to M. bovis strains 428E and PG45"],"prefix":"10.1007","volume":"49","author":[{"given":"Daniel W.","family":"Nielsen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha J.","family":"Hau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Holthausen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohana P.","family":"Dassanayake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan S.","family":"Kaplan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,9,30]]},"reference":[{"issue":"3","key":"10901_CR1","doi-asserted-by":"publisher","first-page":"647","DOI":"10.2460\/javma.1996.209.03.647","volume":"209","author":"DS Adegboye","year":"1996","unstructured":"Adegboye DS, Halbur PG, Nutsch RG, Kadlec RG, Rosenbusch RF (1996) Mycoplasma bovis-associated pneumonia and arthritis complicated with pyogranulomatous tenosynovitis in calves. 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