{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T01:44:35Z","timestamp":1769564675792,"version":"3.49.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1007\/s11259-025-10972-w","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T04:56:40Z","timestamp":1765169800000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sequential changes in serum protein profiles in pregnant Spanish purebred mares"],"prefix":"10.1007","volume":"50","author":[{"given":"Katiuska","family":"Satu\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah La","family":"Fauci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esterina","family":"Fazio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Gemma","family":"Velasco-Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Bruschetta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pietro","family":"Medica","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,8]]},"reference":[{"key":"10972_CR1","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1111\/j.1439-0531.2007.00879.x","volume":"43","author":"R Agr\u00edcola","year":"2008","unstructured":"Agr\u00edcola R, Carvalho H, Barbosa M et al (2008) Blood lymphocyte subpopulations, neutrophil phagocytosis and proteinogram during late pregnancy and postpartum in mares. Reprod Domest Anim 43:212\u2013217. https:\/\/doi.org\/10.1111\/j.1439-0531.2007.00879.x","journal-title":"Reprod Domest Anim"},{"key":"10972_CR2","doi-asserted-by":"publisher","DOI":"10.1186\/s12884-019-2619-6","volume":"19","author":"S Aguree","year":"2019","unstructured":"Aguree S, Gernand AD (2019) Plasma volume expansion across healthy pregnancy: a systematic review and meta-analysis of longitudinal studies. BMC Pregnancy Childbirth 19:508. https:\/\/doi.org\/10.1186\/s12884-019-2619-6","journal-title":"BMC Pregnancy Childbirth"},{"key":"10972_CR3","doi-asserted-by":"publisher","first-page":"105417","DOI":"10.1016\/j.jevs.2025.105417","volume":"149","author":"M Akk\u00f6se","year":"2025","unstructured":"Akk\u00f6se M (2025) The diagnostic accuracy of the Brix refractometer in determining mare colostrum with high and low Immunoglobulin G concentrations. J Equine Vet Sci 149:105417. https:\/\/doi.org\/10.1016\/j.jevs.2025.105417","journal-title":"J Equine Vet Sci"},{"key":"10972_CR4","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1016\/j.jevs.2012.07.012","volume":"33","author":"D Alberghina","year":"2013","unstructured":"Alberghina D, Fazio F, Arfuso F et al (2013) Reference intervals of serum protein concentrations from clinically healthy female Ragusana donkeys (Equus asinus) determined by cellulose acetate electrophoresis. J Equine Vet Sci 33:433\u2013436. https:\/\/doi.org\/10.1016\/j.jevs.2012.07.012","journal-title":"J Equine Vet Sci"},{"key":"10972_CR5","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/j.tvjl.2011.01.019","volume":"191","author":"SA Andersen","year":"2012","unstructured":"Andersen SA, Petersen HH, Ersb\u00f8ll AK et al (2012) Vaccination elicits a prominent acute phase response in horses. Vet J 191:199\u2013202. https:\/\/doi.org\/10.1016\/j.tvjl.2011.01.019","journal-title":"Vet J"},{"key":"10972_CR6","doi-asserted-by":"publisher","DOI":"10.3390\/ani12070862","volume":"12","author":"F Arfuso","year":"2022","unstructured":"Arfuso F, Fazio F, Chikhi L et al (2022) Acute stress response of sheep to shearing procedures: dynamic change of cortisol concentration and protein electrophoretic pattern. Animals 12:862. https:\/\/doi.org\/10.3390\/ani12070862","journal-title":"Animals"},{"key":"10972_CR7","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-37069-1","volume":"13","author":"F Arfuso","year":"2023","unstructured":"Arfuso F, Rizzo M, Giannetto C et al (2023) Inflammatory-like status and acute stress response in horses after road transport. Sci Rep 13:9858. https:\/\/doi.org\/10.1038\/s41598-023-37069-1","journal-title":"Sci Rep"},{"key":"10972_CR8","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1007\/s00580-006-0648-9","volume":"15","author":"RA Batavani","year":"2006","unstructured":"Batavani RA, Ansari MH, Asri S (2006) Concentrations of serum total protein and protein fractions during diestrus and pregnancy in Makuii ewes. Comp Clin Pathol 15:227\u2013230. https:\/\/doi.org\/10.1007\/s00580-006-0648-9","journal-title":"Comp Clin Pathol"},{"key":"10972_CR9","first-page":"53","volume":"34","author":"M Bazzano","year":"2016","unstructured":"Bazzano M, Giudice E, Fazio F et al (2016) Serum protein electrophoresis profile during late pregnancy and early post partum period in mares. Anim Sci Pap Rep 34:53\u201360","journal-title":"Anim Sci Pap Rep"},{"key":"10972_CR10","doi-asserted-by":"publisher","first-page":"1617","DOI":"10.1002\/elps.200500742","volume":"27","author":"F Cairoli","year":"2006","unstructured":"Cairoli F, Battocchio M, Veronesi MC et al (2006) Serum protein pattern during cow pregnancy: Acute-phase proteins increase in the peripartum period. Electrophoresis 27:1617\u20131625. https:\/\/doi.org\/10.1002\/elps.200500742","journal-title":"Electrophoresis"},{"key":"10972_CR11","doi-asserted-by":"publisher","unstructured":"Chaves Rivera MN, Choucair I, Vera MA et al (2025) Haptoglobin in pregnancy: trimester-specific reference intervals. Am J Clin Pathol. https:\/\/doi.org\/10.1093\/ajcp\/aqaf012","DOI":"10.1093\/ajcp\/aqaf012"},{"key":"10972_CR12","doi-asserted-by":"publisher","first-page":"1642","DOI":"10.1093\/clinchem\/27.10.1642","volume":"27","author":"BT Doumas","year":"1981","unstructured":"Doumas BT, Bayse DD, Carter RJ et al (1981) A candidate reference method for determination of total protein in serum. I. Development and validation. Clin Chem 27:1642\u20131650","journal-title":"Clin Chem"},{"key":"10972_CR13","doi-asserted-by":"publisher","first-page":"01010","DOI":"10.1051\/bioconf\/20224901010","volume":"49","author":"N Fitriani","year":"2022","unstructured":"Fitriani N, Astuti P, Mona Airin C et al (2022) Comparison of Albumin\/Globulin (A\/G) ratio between pregnant and lactation of Thin-Tail sheep. BIO Web Conf 49:01010. https:\/\/doi.org\/10.1051\/bioconf\/20224901010","journal-title":"BIO Web Conf"},{"key":"10972_CR14","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1016\/j.tvjl.2014.08.013","volume":"202","author":"N Fouch\u00e9","year":"2014","unstructured":"Fouch\u00e9 N, Graubner C, Howard J (2014) Correlation between serum total globulins and gamma globulins and their use to diagnose failure of passive transfer in foals. Vet J 202:384\u2013386. https:\/\/doi.org\/10.1016\/j.tvjl.2014.08.013","journal-title":"Vet J"},{"key":"10972_CR15","unstructured":"Geor RJ et al (2013) Chap. 6: amino acids and protein. Equine applied and clinical nutrition: Health, welfare and performance. Elsevier"},{"key":"10972_CR16","first-page":"101","volume":"24","author":"M Gianesella","year":"2018","unstructured":"Gianesella M, Perillo L, Fiero E et al (2018) Transition period in healthy and diseased dairy cows: evaluation of metabolic modifications. Large Anim Rev 24:101\u2013107","journal-title":"Large Anim Rev"},{"key":"10972_CR17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2408949121","volume":"121","author":"T Gilboa","year":"2024","unstructured":"Gilboa T, Ter-Ovanesyan D, Wang S-C et al (2024) Measurement of \u03b1-synuclein as protein cargo in plasma extracellular vesicles. Proc Natl Acad Sci USA 121:e2408949121. https:\/\/doi.org\/10.1073\/pnas.2408949121","journal-title":"Proc Natl Acad Sci USA"},{"key":"10972_CR18","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17176275","volume":"17","author":"S G\u00f3mez-Cantarino","year":"2020","unstructured":"G\u00f3mez-Cantarino S, Agull\u00f3-Ortu\u00f1o MT, De Dios-Aguado M et al (2020) Prevalence of hypoproteinemia and hypoalbuminemia in pregnant women from three different socioeconomic populations. Int J Environ Res Public Health 17:6275. https:\/\/doi.org\/10.3390\/ijerph17176275","journal-title":"Int J Environ Res Public Health"},{"key":"10972_CR19","doi-asserted-by":"crossref","unstructured":"Harwood SL, Enghild JJ (2024) Engineering new protease inhibitors using \u03b12-Macroglobulin. In: Santamaria S (ed) Proteases and cancer. Springer US, New York, NY, pp 279\u2013294","DOI":"10.1007\/978-1-0716-3589-6_21"},{"key":"10972_CR20","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1016\/j.tjog.2014.01.007","volume":"54","author":"T-Y Hsu","year":"2015","unstructured":"Hsu T-Y, Hsieh T-T, Yang KD et al (2015) Proteomic profiling reveals \u03b11-antitrypsin, \u03b11-microglobulin, and clusterin as preeclampsia-related serum proteins in pregnant women. Taiwan J Obstet Gynecol 54:499\u2013504. https:\/\/doi.org\/10.1016\/j.tjog.2014.01.007","journal-title":"Taiwan J Obstet Gynecol"},{"key":"10972_CR21","doi-asserted-by":"crossref","unstructured":"Hurley WL, Theil PK (2013) Immunoglobulins in mammary secretions. In: McSweeney PLH, Fox PF (eds) Advanced dairy chemistry. Springer US, Boston, MA, pp 275\u2013294","DOI":"10.1007\/978-1-4614-4714-6_9"},{"key":"10972_CR22","unstructured":"Kaneko JJ, Harvey ML, Bruss ML (2008) Kaneko, J. J., Harvey, J. W., & Bruss, M. L. (2008). Clinical Biochemistry of Domestic Animals, 6th edn. San Diego: Accademic Press"},{"key":"10972_CR23","doi-asserted-by":"crossref","unstructured":"Narayan B, Nelson-Piercy C (2020) Physiological changes of the immune system during pregnancy. In: Einav S, Weiniger CF, Landau R (eds) Principles and practice of maternal critical care. Springer International Publishing, Cham, pp 201\u2013213","DOI":"10.1007\/978-3-030-43477-9_15"},{"key":"10972_CR24","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1017\/S0022029911000562","volume":"78","author":"G Piccione","year":"2011","unstructured":"Piccione G, Messina V, Schembari A et al (2011) Pattern of serum protein fractions in dairy cows during different stages of gestation and lactation. J Dairy Res 78:421\u2013425. https:\/\/doi.org\/10.1017\/S0022029911000562","journal-title":"J Dairy Res"},{"key":"10972_CR25","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1093\/af\/vfad003","volume":"13","author":"AS Reiter","year":"2023","unstructured":"Reiter AS, Reed SA (2023) Lactation in horses. Anim Front 13:103\u2013107. https:\/\/doi.org\/10.1093\/af\/vfad003","journal-title":"Anim Front"},{"key":"10972_CR26","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1111\/j.1939-165X.2008.00100.x","volume":"38","author":"B Riond","year":"2009","unstructured":"Riond B, Wenger-Riggenbach B, Hofmann-Lehmann R, Lutz H (2009) Serum protein concentrations from clinically healthy horses determined by agarose gel electrophoresis. Vet Clin Pathol 38:74\u201379","journal-title":"Vet Clin Pathol"},{"key":"10972_CR27","first-page":"438","volume":"12","author":"D Sai Varun","year":"2023","unstructured":"Sai Varun D, Venkata Ramana K, Chandrashekhar Reddy K, Jagan Mohan Reddy K (2023) Study on changes in haematological and serum biochemical parameters during mid gestation and late gestation in bitches. Pharma Innov 12:438\u2013441","journal-title":"Pharma Innov"},{"key":"10972_CR28","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13328","volume":"85","author":"CA Saito Reis","year":"2021","unstructured":"Saito Reis CA, Padron JG, Norman Ing ND, Kendal-Wright CE (2021) High\u2010mobility group box 1 is a driver of inflammation throughout pregnancy. Am J Reprod Immunol 85:e13328. https:\/\/doi.org\/10.1111\/aji.13328","journal-title":"Am J Reprod Immunol"},{"key":"10972_CR29","doi-asserted-by":"publisher","first-page":"1185","DOI":"10.1016\/j.theriogenology.2010.11.029","volume":"75","author":"K Satu\u00e9","year":"2011","unstructured":"Satu\u00e9 K, Domingo R (2011) Longitudinal study of the renin angiotensin aldosterone system in purebred Spanish broodmares during pregnancy. Theriogenology 75:1185\u20131194. https:\/\/doi.org\/10.1016\/j.theriogenology.2010.11.029","journal-title":"Theriogenology"},{"key":"10972_CR30","doi-asserted-by":"publisher","DOI":"10.1016\/j.vas.2021.100212","volume":"14","author":"K Satu\u00e9","year":"2021","unstructured":"Satu\u00e9 K, Calvo A, Mu\u00f1oz A et al (2021) Interrelationship between reproductive hormones and acute phase proteins during estrous cycle and pregnancy in Spanish purebred broodmares. Vet Anim Sci 14:100212. https:\/\/doi.org\/10.1016\/j.vas.2021.100212","journal-title":"Vet Anim Sci"},{"key":"10972_CR31","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2022.104023","volume":"115","author":"K Satu\u00e9","year":"2022","unstructured":"Satu\u00e9 K, Fazio E, Mu\u00f1oz A, Medica P (2022) Successful pregnancy outcome in mares: the potential role of body conditional score, age and biochemical parameter\u2019s adjustments. J Equine Vet Sci 115:104023. https:\/\/doi.org\/10.1016\/j.jevs.2022.104023","journal-title":"J Equine Vet Sci"},{"key":"10972_CR32","doi-asserted-by":"publisher","first-page":"197","DOI":"10.5194\/aab-66-197-2023","volume":"66","author":"K Satu\u00e9","year":"2023","unstructured":"Satu\u00e9 K, Fazio E, La Fauci D, Medica P (2023) Hematological indexes and iron status in pregnant mares. Arch Anim Breed 66:197\u2013205. https:\/\/doi.org\/10.5194\/aab-66-197-2023","journal-title":"Arch Anim Breed"},{"key":"10972_CR33","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1590\/1678-4162-10102","volume":"71","author":"AF Souza","year":"2019","unstructured":"Souza AF, Schade J, Ramos AF et al (2019) Serum proteinogram of the Campeiro horse. Arq Bras Med Vet Zootec 71:363\u2013368. https:\/\/doi.org\/10.1590\/1678-4162-10102","journal-title":"Arq Bras Med Vet Zootec"},{"key":"10972_CR34","first-page":"370","volume-title":"Fundamentals of veterinary clinical pathology","author":"S Stockham","year":"2008","unstructured":"Stockham S, Scott MA (2008) Proteins. Fundamentals of veterinary clinical pathology, 2nd edn. Blackwell Publishing, Oxford, UK, pp 370\u2013405","edition":"2"},{"key":"10972_CR35","doi-asserted-by":"publisher","first-page":"486","DOI":"10.58292\/ct.v12.9447","volume":"12","author":"J Storme","year":"2020","unstructured":"Storme J, McCue P, Bisiau C, Hess A (2020) Brix refractometer scoring of immunoglobulin G in postpartum mares\u2019 colostrum. CT 12:486\u2013492. https:\/\/doi.org\/10.58292\/ct.v12.9447","journal-title":"CT"},{"key":"10972_CR36","doi-asserted-by":"publisher","first-page":"1783","DOI":"10.1111\/jog.14365","volume":"46","author":"A Strawa","year":"2020","unstructured":"Strawa A, Skar\u017cy\u0144ska E, Zborowska H et al (2020) Can variability of serum electrophoretic fractions during pregnancy provide knowledge about maternal and fetal health. J Obstet Gynaecol 46:1783\u20131789. https:\/\/doi.org\/10.1111\/jog.14365","journal-title":"J Obstet Gynaecol"},{"key":"10972_CR37","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.theriogenology.2017.04.011","volume":"97","author":"M Szczubia\u0142","year":"2017","unstructured":"Szczubia\u0142 M, Wawrzykowski J, D\u0105browski R et al (2017) Preliminary study on plasma proteins in pregnant and non-pregnant female dogs. Theriogenology 97:1\u20138. https:\/\/doi.org\/10.1016\/j.theriogenology.2017.04.011","journal-title":"Theriogenology"},{"key":"10972_CR38","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.mpaic.2016.04.010","volume":"17","author":"L Talbot","year":"2016","unstructured":"Talbot L, Maclennan K (2016) Physiology of pregnancy. Anaesth Intensive Care Med 17:341\u2013345. https:\/\/doi.org\/10.1016\/j.mpaic.2016.04.010","journal-title":"Anaesth Intensive Care Med"},{"key":"10972_CR39","doi-asserted-by":"publisher","first-page":"475","DOI":"10.17221\/19\/2016-VETMED","volume":"61","author":"C Tothova","year":"2016","unstructured":"Tothova C, Nagy O, Kovac G (2016) Serum proteins and their diagnostic utility in veterinary medicine: a review. Vet Med 61:475\u2013496. https:\/\/doi.org\/10.17221\/19\/2016-VETMED","journal-title":"Vet Med"},{"key":"10972_CR40","first-page":"140","volume":"1","author":"NE Udeh","year":"2021","unstructured":"Udeh NE, Orji E, Madubuike AJ et al (2021) Effect of season, age and sex on haematology and serum biochemistry of sport horses. J Vet Anim Sci (JoSVAS) 1:140\u2013144","journal-title":"J Vet Anim Sci (JoSVAS)"},{"key":"10972_CR41","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/S0378-4320(02)00160-4","volume":"74","author":"CI Vannucchi","year":"2002","unstructured":"Vannucchi CI, Mirandola RM, Oliveira CM (2002) Acute-phase protein profile during gestation and diestrous: proposal for an early pregnancy test in bitches. Anim Reprod Sci 74:87\u201399. https:\/\/doi.org\/10.1016\/S0378-4320(02)00160-4","journal-title":"Anim Reprod Sci"},{"key":"10972_CR42","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1590\/S0100-736X2016000800017","volume":"36","author":"CI Vannucchi","year":"2016","unstructured":"Vannucchi CI, L\u00facio CF, Regazzi FM et al (2016) Perfil de prote\u00ednas plasm\u00e1ticas Em Cadelas gestantes e n\u00e3o gestantes. Pesq Vet Bras 36:781\u2013786. https:\/\/doi.org\/10.1590\/S0100-736X2016000800017","journal-title":"Pesq Vet Bras"},{"key":"10972_CR43","first-page":"21","volume":"70","author":"Z Zvorc","year":"2000","unstructured":"Zvorc Z, Matijatko V, Beer B et al (2000) Blood serum proteinograms in pregnant and non-pregnant cows. Veterinarski Arhiv 70:21\u201330","journal-title":"Veterinarski Arhiv"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-025-10972-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-025-10972-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-025-10972-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T13:00:32Z","timestamp":1769518832000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11259-025-10972-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,8]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10972"],"URL":"https:\/\/doi.org\/10.1007\/s11259-025-10972-w","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,8]]},"assertion":[{"value":"1 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 October 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All methods and procedures used in the present study followed the guidelines of Spanish law (RD 37\/2014) that regulates the protection of animals used for scientific purposes. The Animal Ethics Committee for the Care and Use of Animals of the CEU-Cardenal Herrera University (Spain) concluded that the proposed study did not need ethical approval, since this experiment was part of the clinical evaluation of the animals at this stage of their cycle.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Informed consent was obtained from the owners of all subjects involved in the study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent statement"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"65"}}