{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T01:44:37Z","timestamp":1769564677722,"version":"3.49.0"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council for Medical Research","doi-asserted-by":"crossref","award":["Ref. No. ZON\/43\/2019-ECD-II"],"award-info":[{"award-number":["Ref. No. ZON\/43\/2019-ECD-II"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1007\/s11259-025-10997-1","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T03:00:55Z","timestamp":1764903655000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First molecular genotyping of Coxiella burnetii associated reproductive disorders in domestic ruminants, Tamil Nadu, India"],"prefix":"10.1007","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0135-5957","authenticated-orcid":false,"given":"Sithanadam","family":"Rajagunalan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manavalan","family":"Aravind","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumarasekar","family":"Jeyashree","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annadurai","family":"Sundar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajevar","family":"Ganesan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanmugasamy","family":"Malmarugan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"10997_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2180-6-38","volume":"6","author":"N Arricau-Bouvery","year":"2006","unstructured":"Arricau-Bouvery N, Hauck Y, Bejaoui A, Frangoulidis D, Bodier CC, Souriau A, Meyer H, Neubauer H, Vergnaud G (2006) Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing. BMC Microbiol 6:1\u201314","journal-title":"BMC Microbiol"},{"key":"10997_CR2","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1016\/j.micinf.2015.09.029","volume":"17","author":"L Ceglie","year":"2015","unstructured":"Ceglie L, Guerrini E, Rampazzo E, Barberio A, Tilburg JJHC, Hagen F, Lucchese L, Zuliani F, Marangon S, Natale A (2015) Molecular characterization by MLVA of Coxiella burnetii strains infecting dairy cows and goats of north-eastern Italy. Microbes Infect 17:776\u2013781","journal-title":"Microbes Infect"},{"key":"10997_CR3","unstructured":"Cumbass\u00e1 A (2013) Sources and routes of transmission of Q fever: detection, identification and molecular typing of Coxiella burnetti in domestic and wild animals (Doctoral dissertation). Universidade De Lisboa, Portugal"},{"key":"10997_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12917-018-1499-8","volume":"14","author":"M Di Domenico","year":"2018","unstructured":"Di Domenico M, Curini V, Di Lollo V, Massimini M, Di Gialleonardo L, Franco A, Caprioli A, Battisti A, Camm\u00e0 C (2018) Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy. BMC Vet Res 14:1\u20137","journal-title":"BMC Vet Res"},{"key":"10997_CR5","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1128\/CMR.00045-16","volume":"30","author":"C Eldin","year":"2017","unstructured":"Eldin C, M\u00e9lenotte C, Mediannikov O, Ghigo E, Million M, Edouard S, Mege JL, Maurin M, Raoult D (2017) From Q fever to Coxiella burnetii infection: a paradigm change. Clin Microbiol Rev 30:115\u2013190","journal-title":"Clin Microbiol Rev"},{"key":"10997_CR6","volume-title":"New frontiers of molecular epidemiology of infectious diseases","author":"M Hallin","year":"2012","unstructured":"Hallin M, Deplano A, Struelens MJ (2012) Molecular typing of bacterial pathogens: A tool for the epidemiological study and control of infectious diseases. In: Morand S, Beaudeau F, Cabaret J (eds) New frontiers of molecular epidemiology of infectious diseases. Springer, Dordrecht"},{"key":"10997_CR7","doi-asserted-by":"publisher","first-page":"105081","DOI":"10.1016\/j.prevetmed.2020.105081","volume":"181","author":"R Keshavamurthy","year":"2020","unstructured":"Keshavamurthy R, Singh BB, Kalambhe DG, Aulakh RS, Dhand NK (2020) Identification of risk factors associated with Coxiella burnetii infection in cattle and buffaloes in India. Prev Vet Med 181:105081","journal-title":"Prev Vet Med"},{"key":"10997_CR8","doi-asserted-by":"crossref","unstructured":"Massung RF, Cutler SJ, Frangoulidis D (2012) Molecular typing of Coxiella burnetii (Q Fever). In: Toman R, Heinzen R, Samuel J, Mege JL (eds) Coxiella burnetii: recent advances and new perspectives in research of the Q fever bacterium. Adv Exp Med Biol, vol 984. Springer, Dordrecht","DOI":"10.1007\/978-94-007-4315-1_19"},{"key":"10997_CR9","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.meegid.2015.02.006","volume":"31","author":"A Pi\u00f1ero","year":"2015","unstructured":"Pi\u00f1ero A, Barandika JF, Garc\u00eda-P\u00e9rez AL, Hurtado A (2015) Genetic diversity and variation over time of Coxiella burnetii genotypes in dairy cattle and the farm environment. Infect Genet Evol 31:231\u2013235","journal-title":"Infect Genet Evol"},{"key":"10997_CR10","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1016\/j.micinf.2015.09.026","volume":"17","author":"M Prigent","year":"2015","unstructured":"Prigent M, Rousset E, Yang E, Thi R, Sidi-boumedine K (2015) Validation study for using lab-on-chip technology for Coxiella burnetii multi-locus-VNTR-analysis (MLVA) typing: application for studying genotypic diversity of strains from domestic ruminants in France. Microbes Infect 17:782\u2013788","journal-title":"Microbes Infect"},{"key":"10997_CR11","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1007\/s11250-018-1756-7","volume":"51","author":"S Rajagunalan","year":"2019","unstructured":"Rajagunalan S, Gururaj K, Lakshmikantan U, Murugan M, Ganesan A, Sundar A, Sureshkannan S, Andani D, Pawaiya RS (2019) Detection of the presence of Coxiella burnetii in a case of goat abortion: a first report from India. Trop Anim Health Prod 51:983\u2013986","journal-title":"Trop Anim Health Prod"},{"key":"10997_CR20","unstructured":"Ranjbar R, Karami A, Farshad S, Giammanco GM, Mammina C (2014) Typing methods used in the molecular epidemiology of microbial pathogens: a how-to-guide. New Microbiol. 37:1\u201315.\u00a0https:\/\/pubmed.ncbi.nlm.nih.gov\/24531166\/"},{"key":"10997_CR12","doi-asserted-by":"publisher","first-page":"668","DOI":"10.3201\/eid1704.101562","volume":"17","author":"HI Roest","year":"2011","unstructured":"Roest HI, Ruuls RC, Tilburg JJ, Nabuurs-Franssen MH, Klaassen CH, Vellema P, van den Brom R, Dercksen D, Wouda W, Spierenburg MA, van der Spek AN, Buijs R, de Boer AG, Willemsen PT, van Zijderveld FG (2011) Molecular epidemiology of Coxiella burnetii from ruminants in Q fever outbreak, the Netherlands. Emerg Infect Dis 17:668\u2013675","journal-title":"Emerg Infect Dis"},{"key":"10997_CR13","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1111\/1758-2229.12918","volume":"13","author":"R Sahu","year":"2021","unstructured":"Sahu R, Rawool DB, Dhaka P, Yadav JP, Mishra SP, Kumar M, Vergis J, Malik SS, Barbuddhe SB (2021) Current perspectives on the occurrence of Q fever: highlighting the need for systematic surveillance for a neglected zoonotic disease in Indian Subcontinent. Environ Microbiol Rep 13:138\u2013158","journal-title":"Environ Microbiol Rep"},{"key":"10997_CR14","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1111\/j.1469-0691.2008.02271.x","volume":"15","author":"K Sidi-Boumedine","year":"2009","unstructured":"Sidi-Boumedine K, Duquesne V, Fernandes I, Marro S, Thi\u00e9ry R (2009) Evaluation of randomly amplified polymorphic DNA (RAPD) for discrimination of Coxiella burnetii ruminant strains isolated in France. Clin Microbiol Infect 15:194\u2013195","journal-title":"Clin Microbiol Infect"},{"key":"10997_CR15","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1016\/j.micinf.2015.08.009","volume":"17","author":"K Sidi-Boumedine","year":"2015","unstructured":"Sidi-Boumedine K, Duquesne V, Prigent M, Yang E, Jouli\u00e9 A, Thi\u00e9ry R, Rousset E (2015) Impact of IS1111 insertion on the MLVA genotyping of Coxiella burnetii. Microbes Infect 17:789\u2013794","journal-title":"Microbes Infect"},{"key":"10997_CR16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0210244","volume":"14","author":"M Szyma\u0144ska-Czerwi\u0144ska","year":"2019","unstructured":"Szyma\u0144ska-Czerwi\u0144ska M, Jode\u0142ko A, Zar\u0119ba-Marchewka K, Niemczuk K (2019) Shedding and genetic diversity of Coxiella burnetii in Polish dairy cattle. PLoS ONE 14:1\u201316","journal-title":"PLoS ONE"},{"key":"10997_CR17","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.1128\/JCM.05497-11","volume":"50","author":"JJ Tilburg","year":"2012","unstructured":"Tilburg JJ, Rossen JW, van Hannen EJ, Melchers WJ, Hermans MH, van de Bovenkamp J, Roest HJI, de Bruin A, Nabuurs-Franssen MH, Horrevorts AM, Klaassen CH (2012) Genotypic diversity of Coxiella burnetii in the 2007\u20132010 Q fever outbreak episodes in the Netherlands. J Clin Microbiol 50:1076\u20131078","journal-title":"J Clin Microbiol"},{"key":"10997_CR18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fcimb.2020.625576","volume":"10","author":"S Tomaiuolo","year":"2021","unstructured":"Tomaiuolo S, Boarbi S, Fancello T, Michel P, Desqueper D, Gr\u00e9goire F, Callens J, Fretin D, Devriendt B, Cox E, Mori M (2021) Phylogeography of human and animal Coxiella burnetii strains: genetic fingerprinting of Q fever in Belgium. Front Cell Infect Microbiol 10:1\u201315","journal-title":"Front Cell Infect Microbiol"},{"key":"10997_CR19","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1016\/j.ijmm.2016.05.014","volume":"306","author":"G Vincent","year":"2016","unstructured":"Vincent G, Stenos J, Latham J, Fenwick S, Graves S (2016) Novel genotypes of Coxiella burnetii identified in isolates from Australian Q fever patients. Int J Med Microbiol 306:463\u2013470","journal-title":"Int J Med Microbiol"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-025-10997-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-025-10997-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-025-10997-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T13:00:50Z","timestamp":1769518850000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11259-025-10997-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,5]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10997"],"URL":"https:\/\/doi.org\/10.1007\/s11259-025-10997-1","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,5]]},"assertion":[{"value":"26 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The research was approved by the appropriate Institutional Biosafety Committee, Faculty of Basic Science, Madras Veterinary College Campus, Chennai. The research work approved by the Registrar, TANUVAS under the USO No. 50164\/G2\/2021 Proc.No. 13721\/G2\/2019. Samples were collected as part of veterinary care by a well-trained veterinary practitioner from the Department of Veterinary Gynecology and Obstetrics, Veterinary College and Research Institute, TANUVAS, Tirunelveli, Tamil Nadu, India. The sample collection was carried out strictly adhering to the guidelines and regulations of the Committee for the Control and Supervision of Experiments on Animals (2018) and Breeding of and Experiments on Animals (control and supervision) Rules, 2006. No extra discomfort was caused during sample collection. The collection of the material is considered as a routine veterinary practice to ensure the well-being of animal and is non- experimental research. Therefore, did not require ethical approval by the animal experimentation committee. Informed oral consent was obtained from the owner at the time of sampling. All the performed methods were in accordance with the ARRIVE guidelines and regulations.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"63"}}