{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T03:15:05Z","timestamp":1772939705445,"version":"3.50.1"},"reference-count":18,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No.82274013"],"award-info":[{"award-number":["No.82274013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"crossref","award":["No. 2632025TD03"],"award-info":[{"award-number":["No. 2632025TD03"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1007\/s11259-025-11058-3","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T07:27:10Z","timestamp":1770708430000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and validation of UPLC-MS\/MS method for pharmacokinetics and tissue distribution of Tylvalosin and its bioactive metabolites in swine"],"prefix":"10.1007","volume":"50","author":[{"given":"Xianhai","family":"Luo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangshan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yindi","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaonan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahui","family":"Tu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjun","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nini","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuexin","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhixia","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,2,10]]},"reference":[{"key":"11058_CR1","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1111\/jvp.13116","volume":"46","author":"EA Abu-Basha","year":"2023","unstructured":"Abu-Basha EA, Bani Ismail Z, Idkaidek NM, Hamzeh E (2023) Comparison of pharmacokinetics of two Tylvalosin oral formulations in broiler chickens. J Vet Pharmacol Ther 46:165\u2013169. https:\/\/doi.org\/10.1111\/jvp.13116","journal-title":"J Vet Pharmacol Ther"},{"key":"11058_CR2","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1111\/jvp.12309","volume":"39","author":"P Canning","year":"2016","unstructured":"Canning P, Bates J, Hammen K, Coetzee J, Wulf L, Rajewski S, Wang C, Karriker L (2016) Concentrations of Tylvalosin and 3-O-acetyltylosin attained in the synovial fluid of swine after administration by oral gavage at 50 and 5\u00a0mg\/kg. J Vet Pharmacol Ther 39:621\u2013624. https:\/\/doi.org\/10.1111\/jvp.12309","journal-title":"J Vet Pharmacol Ther"},{"key":"11058_CR3","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2019.00355","volume":"6","author":"M Elbadawy","year":"2019","unstructured":"Elbadawy M, Aboubakr M, Abugomaa A (2019) Pharmacokinetics of Tylvalosin in broiler turkeys (Meleagris gallopavo) after single intravenous and oral administration. Front Vet Sci 6:355. https:\/\/doi.org\/10.3389\/fvets.2019.00355","journal-title":"Front Vet Sci"},{"key":"11058_CR4","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1136\/vr.165.12.342","volume":"165","author":"RM Guedes","year":"2009","unstructured":"Guedes RM, Fran\u00e7a SA, Machado GS, Blumer MA, da Costa Cruz EC Jr. (2009) Use of tylvalosin-medicated feed to control porcine proliferative enteropathy. Vet Rec 165:342\u20135. https:\/\/doi.org\/10.1136\/vr.165.12.342","journal-title":"Vet Rec"},{"key":"11058_CR5","unstructured":"ingle L, Zhiqiang N, Xinguo L, Xiaodong Y, Xuepeng Z, Jiang M, Zhenghai Z (2020) The preparation method and application of 3-acetyl desmycosin medicinal salt (China Patent No. CN110684065B). China National Intellectual Property Administration (CNIPA)."},{"key":"11058_CR6","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2021.6858","volume":"19","author":"K Koutsoumanis","year":"2021","unstructured":"Koutsoumanis K (2021) Maximum levels of cross-contamination for 24 antimicrobial active substances in non-target feed. Part 6: macrolides: tilmicosin, tylosin and tylvalosin. EFSA J 19:e06858. https:\/\/doi.org\/10.2903\/j.efsa.2021.6858","journal-title":"EFSA J"},{"key":"11058_CR7","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2018.00057","volume":"5","author":"R Moges","year":"2018","unstructured":"Moges R, De Lamache DD, Sajedy S, Renaux BS, Hollenberg MD, Muench G, Abbott EM, Buret AG (2018) Anti-inflammatory benefits of antibiotics: tylvalosin induces apoptosis of porcine neutrophils and macrophages, promotes efferocytosis, and inhibits pro-inflammatory CXCL-8, IL1\u03b1, and LTB(4) production, while inducing the release of pro-resolving lipoxin A(4) and resolvin D1. Front Vet Sci 5:57. https:\/\/doi.org\/10.3389\/fvets.2018.00057","journal-title":"Front Vet Sci"},{"key":"11058_CR8","doi-asserted-by":"publisher","first-page":"1322012","DOI":"10.3389\/fgeed.2024.1322012","volume":"6","author":"C Nesbitt","year":"2024","unstructured":"Nesbitt C, Galina Pantoja L, Beaton B, Chen CY, Culbertson M, Harms P, Holl J, Sosnicki A, Reddy S, Rotolo M, Rice E (2024) Pigs lacking the SRCR5 domain of CD163 protein demonstrate heritable resistance to the PRRS virus and no changes in animal performance from birth to maturity. Front Genome Ed 6:1322012. https:\/\/doi.org\/10.3389\/fgeed.2024.1322012","journal-title":"Front Genome Ed"},{"key":"11058_CR9","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/j.tvjl.2014.02.028","volume":"200","author":"S Py\u00f6r\u00e4l\u00e4","year":"2014","unstructured":"Py\u00f6r\u00e4l\u00e4 S, Baptiste KE, Catry B, van Duijkeren E, Greko C, Moreno MA, Pomba MCMF, Rantala M, Ru\u017eauskas M, Sanders P, Threlfall EJ, Torren-Edo J, T\u00f6rneke K (2014) Macrolides and Lincosamides in cattle and pigs: use and development of antimicrobial resistance. Vet J 200:230\u2013239. https:\/\/doi.org\/10.1016\/j.tvjl.2014.02.028","journal-title":"Vet J"},{"key":"11058_CR10","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1186\/s12917-023-03571-x","volume":"19","author":"AL Rodriguez","year":"2023","unstructured":"Rodriguez AL, Fowler VL, Huether M, Reddick D, Tait-Burkard C, O\u2019Shea M, Perkins S, Dias N, Buterbaugh R, Benchaoui HA (2023) Effects of a water-soluble formulation of Tylvalosin on disease caused by Porcine reproductive and respiratory syndrome virus alone in sows or in combination with Mycoplasma hyopneumoniae in piglets. BMC Vet Res 19:31. https:\/\/doi.org\/10.1186\/s12917-023-03571-x","journal-title":"BMC Vet Res"},{"key":"11058_CR11","doi-asserted-by":"publisher","unstructured":"Rosales RS, Ram\u00edrez AS, Tav\u00edo MM, Poveda C, Poveda JB (2020) Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe. BMC Vet Res 16. https:\/\/doi.org\/10.1186\/s12917-020-02512-2","DOI":"10.1186\/s12917-020-02512-2"},{"key":"11058_CR12","first-page":"42","volume":"61","author":"A Stuart","year":"2008","unstructured":"Stuart A, Brown TDK, Mockett APA (2008) Tylvalosin, a macrolide antibiotic, inhibits the in vitro replication of European and American Porcine reproductive and respiratory syndrome virus (PRRS) viruses. Pig J 61:42\u201348","journal-title":"Pig J"},{"key":"11058_CR13","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1136\/vr.102458","volume":"175","author":"MM Tav\u00edo","year":"2014","unstructured":"Tav\u00edo MM, Poveda C, Assun\u00e7\u00e3o P, Ram\u00edrez AS, Poveda JB (2014) In vitro activity of Tylvalosin against Spanish field strains of Mycoplasma hyopneumoniae. Vet Rec 175:539\u2013539. https:\/\/doi.org\/10.1136\/vr.102458","journal-title":"Vet Rec"},{"key":"11058_CR14","doi-asserted-by":"publisher","unstructured":"Wen Z, Chen S, Meng J, Wu Q, Yu R, Xu N, Kong J, Zhang L, Cao X (2025) Pharmacokinetics of Tylvalosin Following Intravenous or Oral Administration at Different Doses in Broiler Chickens. Vet Sci 12. https:\/\/doi.org\/10.3390\/vetsci12020118","DOI":"10.3390\/vetsci12020118"},{"key":"11058_CR15","doi-asserted-by":"publisher","first-page":"2283","DOI":"10.1007\/s11696-017-0222-8","volume":"71","author":"J-X Wu","year":"2017","unstructured":"Wu J-X, Zhang C-L, Xu J-L, Zhang G-D, Hao J, Zhang M, Song M, Qi P, Zhang G, Liang J-L (2017) Bioconversion, purification and characterization of Tylvalosin. Chem Pape 71:2283\u20132291. https:\/\/doi.org\/10.1007\/s11696-017-0222-8","journal-title":"Chem Pape"},{"key":"11058_CR16","doi-asserted-by":"publisher","unstructured":"Zhang Q, Cui C, Zhang S, Deng X, Cai X, Wang G (2022) Tylvalosin tartrate improves the health status of swine herds during immunization with Porcine reproductive and respiratory syndrome Virus-Inactivated vaccine. Vet Sci 10. https:\/\/doi.org\/10.3390\/vetsci10010012","DOI":"10.3390\/vetsci10010012"},{"key":"11058_CR17","doi-asserted-by":"publisher","unstructured":"Zheng S, An H, Xu F, Meng F, Cai X, Tian Z, Peng J, Deng X, Shang\u00a0Y, Wang G (2024) Tylvalosin tartrate inhibits the replication stage of the Porcine reproductive and respiratory syndrome virus life cycle. Microb Pathog 196:106988. https:\/\/doi.org\/10.1016\/j.micpath.2024.106988","DOI":"10.1016\/j.micpath.2024.106988"},{"key":"11058_CR18","doi-asserted-by":"publisher","unstructured":"Zhuang Y, Sun Q, Jing T, Liu J, Meng H, Cao Y, Qiu Z, Sun J, Li N (2022) Contributions of intestine and liver to the absorption and\u00a0disposition of FZJ-003, a selective JAK1 inhibitor with structure modification of filgotinib. Eur J Pharm Sci 175:106211. https:\/\/doi.org\/10.1016\/j.ejps.2022.106211","DOI":"10.1016\/j.ejps.2022.106211"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-025-11058-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-025-11058-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-025-11058-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T09:48:59Z","timestamp":1772876939000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11259-025-11058-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,10]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["11058"],"URL":"https:\/\/doi.org\/10.1007\/s11259-025-11058-3","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,10]]},"assertion":[{"value":"23 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 December 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 February 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experiments were performed in Shandong Huahong Biological Engineering Co., Ltd., and accordance with the protocols approved by the Animal Care and Ethics Committee of the Shandong Huahong Biological Engineering Co., Ltd., which is organized and operated in accordance with the Chinese national guidelines for animal welfare. The approval number for this study is 202409003. The swine used in this study were purchased commercially from Binzhou Huayu Swine Breeding Co., Ltd. North Chen Branch. The commercial nature of this transaction constitutes consent for the use of these animals in research.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"154"}}