{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T18:54:50Z","timestamp":1781636090225,"version":"3.54.5"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:00:00Z","timestamp":1771027200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:00:00Z","timestamp":1771027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universidade Federal Rural De Pernambuco"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to identify\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    in healthy milk, mastitic milk, and rectal swab samples from dairy goats and kids in herds located in the state of Pernambuco, Brazil, and to evaluate the phenotypic profile of ESBL-producing isolates, as well as to detect the genotypic expression of ESBL resistance genes and virulence factors. Rectal swab and milk samples were collected from 56 goats and 14 kids. Gram-negative bacillary colonies were identified using Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). The double-disk synergy test (DDST) for the detection of extended-spectrum \u03b2-lactamases (ESBL) was performed using the antibiotic discs ceftazidime, ceftriaxone, cefotaxime, aztreonam, and amoxicillin\/clavulanic acid. Genotypic profiling for ESBL and virulence factor genes targeted\n                    <jats:italic>blaSHV<\/jats:italic>\n                    ,\n                    <jats:italic>blaTEM<\/jats:italic>\n                    ,\n                    <jats:italic>blaCTX-M<\/jats:italic>\n                    ,\n                    <jats:italic>stx1<\/jats:italic>\n                    ,\n                    <jats:italic>stx2<\/jats:italic>\n                    ,\n                    <jats:italic>eaeA<\/jats:italic>\n                    , and\n                    <jats:italic>hlyA<\/jats:italic>\n                    .\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    spp.,\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    spp., and\n                    <jats:italic>Cronobacter<\/jats:italic>\n                    sp. were identified. The phenotypic test revealed that 27% (25\/92) of the isolates were ESBL-positive. In the genotypic analysis, one isolate carried the\n                    <jats:italic>blaSHV<\/jats:italic>\n                    gene and one carried the\n                    <jats:italic>blaTEM<\/jats:italic>\n                    gene, while\n                    <jats:italic>blaCTX-M<\/jats:italic>\n                    was not detected. At least one virulence-associated gene (\n                    <jats:italic>hlyA<\/jats:italic>\n                    ,\n                    <jats:italic>stx1<\/jats:italic>\n                    , and\n                    <jats:italic>stx2<\/jats:italic>\n                    ) was detected in 32% (30\/92) of the isolates. The detection of antimicrobial resistance and virulence factors in enteric bacteria from healthy goat herds highlights potential risks to human health and animal welfare, as well as possible economic losses in rural farming systems.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11102-w","type":"journal-article","created":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T08:11:48Z","timestamp":1771056708000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["ESBL-producing and virulent enteric bacteria from goats in Northeastern Brazil"],"prefix":"10.1007","volume":"50","author":[{"given":"Guilherme Valeriano","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denny Parente de S\u00e1 Barreto Maia","family":"Leite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucilene Martins Trindade","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pollyanne Raysa Fernandes","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"de Souza Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Aparecida","family":"Juliano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Wilton","family":"Pinheiro Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinaldo Aparecido","family":"Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,14]]},"reference":[{"key":"11102_CR29","doi-asserted-by":"publisher","unstructured":"Annavajhala MK, Gomez-Simmonds A, Uhlemann AC (2019) Multidrug-resistant Enterobacter cloacae complex emerging as a global, diversifying threat. 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