{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T18:56:23Z","timestamp":1781636183749,"version":"3.54.5"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100012834","name":"Gifu University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100012834","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Respiratory infections are major health concerns in wild and managed cetaceans, yet reliable methods for assessing lower respiratory tract inflammation are limited. We aimed to evaluate the clinical utility of bronchoalveolar lavage fluid (BALF) neutrophil differentials in three managed bottlenose dolphins (\n                    <jats:italic>Tursiops truncatus<\/jats:italic>\n                    ) and three managed beluga whales (\n                    <jats:italic>Delphinapterus leucas<\/jats:italic>\n                    ) undergoing treatment for respiratory infections. Infection status was monitored by assessing isolated bacteria and fungi from BALF, BALF neutrophil differentials, and inflammatory markers in the blood, such as total leukocyte counts, plasma fibrinogen levels, and serum iron levels. In animals with respiratory bacterial and\/or fungal infections alone, both BALF neutrophil differentials (47.3\u201355.7%) and blood inflammatory markers were elevated. Following antimicrobial treatment, the differentials decreased (0.8\u20133.8%), and blood inflammatory markers also returned to normal ranges. Contrastingly, in animals with concurrent conditions, such as respiratory bacterial and\/or fungal infections accompanied by wounds on the tail fin or infection-induced iron deficiency anemia, persistent blood inflammatory markers were observed after antimicrobial treatment. Despite this, BALF neutrophil differentials, which are specific to respiratory inflammation, decreased in response to treatment (3.1\u201321.6%). Moreover, microorganisms isolated from blow samples from these animals were not consistent with those isolated from BALF at the same time. These findings suggest that BALF reflects localized respiratory infection more accurately than the inflammatory markers in the blood, and BALF examination is useful for diagnosing microbial infections in the lower respiratory tract in cetaceans. This approach may improve clinical decision-making and therapeutic outcomes in managed cetacean populations worldwide.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11112-8","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T09:38:56Z","timestamp":1771580336000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Neutrophil differentials in bronchoalveolar lavage fluid in bottlenose dolphins (Tursiops truncatus) and beluga whales (Delphinapterus leucas) during treatment of respiratory infection: a preliminary study"],"prefix":"10.1007","volume":"50","author":[{"given":"Takashi","family":"Kamio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukako","family":"Odani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Kanda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Mori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichiro","family":"Akune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wataru","family":"Ohtomo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Kurita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaka","family":"Okada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Inoshima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,20]]},"reference":[{"key":"11112_CR1","doi-asserted-by":"publisher","first-page":"17","DOI":"10.3354\/dao","volume":"138","author":"A Borque-Espinosa","year":"2020","unstructured":"Borque-Espinosa A, Burgos F, Dennison S, Laughlin R, Manley M, Azzati RC, Fahlman A (2020) Pulmonary function testing as a diagnostic tool to assess respiratory health in bottlenose dolphins Tursiops truncatus. 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