{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T03:15:11Z","timestamp":1772939711623,"version":"3.50.1"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"the Natural Science Foundation of Guangdong Province","award":["2020A1515110362"],"award-info":[{"award-number":["2020A1515110362"]}]},{"name":"the Science and Technology Program of XPCC","award":["2024DA006"],"award-info":[{"award-number":["2024DA006"]}]},{"name":"the Marine Biology Foundation of Zhanjiang Bureau of Science and Technology","award":["2021E05028"],"award-info":[{"award-number":["2021E05028"]}]},{"name":"the Guangdong Ocean University Student Innovation Training Program","award":["S202410566029"],"award-info":[{"award-number":["S202410566029"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1007\/s11259-026-11114-6","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T11:52:57Z","timestamp":1771501977000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of the characteristics of microRNA expression profiles and reproductive regulation-mediated signalling pathways in testicular sertoli derived sEVs from Leizhou goats"],"prefix":"10.1007","volume":"50","author":[{"given":"Yang","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowen","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huimingda","family":"Bao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongnan","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangdong","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"11114_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.121251","volume":"312","author":"N Amiri","year":"2023","unstructured":"Amiri N, Mohammadi P, Allahgholi A et al (2023) The potential of sertoli cells (SCs) derived exosomes and its therapeutic efficacy in male reproductive disorders. Life Sci 312:121251. https:\/\/doi.org\/10.1016\/j.lfs.2022.121251","journal-title":"Life Sci"},{"key":"11114_CR2","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2025.1560915","volume":"13","author":"J Chen","year":"2025","unstructured":"Chen J, Liu S, Zou J et al (2025) Comparison of efficacy of exosomes derived from human umbilical cord blood mesenchymal stem cells in treating mouse acute lung injury via different routes. Front Pediatr 13:1560915. https:\/\/doi.org\/10.3389\/fped.2025.1560915","journal-title":"Front Pediatr"},{"key":"11114_CR3","doi-asserted-by":"publisher","DOI":"10.34172\/bi.30801","volume":"15","author":"F Dashti","year":"2025","unstructured":"Dashti F, Moghaddaszadeh-Ahrabi S, Maleki M, Firouzi-Amoudizaj F (2025) The effect of Sertoli cell exosomes on spermatogonia stem cells differentiation. BioImpacts 15:30801. https:\/\/doi.org\/10.34172\/bi.30801","journal-title":"BioImpacts"},{"key":"11114_CR4","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.16875","volume":"12","author":"R Deng","year":"2024","unstructured":"Deng R, Wu Z, He C et al (2024) Exosomes from uterine fluid promote capacitation of human sperm. PeerJ 12:e16875. https:\/\/doi.org\/10.7717\/peerj.16875","journal-title":"PeerJ"},{"key":"11114_CR5","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.theriogenology.2023.02.011","volume":"200","author":"H Gao","year":"2023","unstructured":"Gao H, Cao H, Li Z et al (2023) Exosome-derived small RNAs in mouse Sertoli cells inhibit spermatogonial apoptosis. Theriogenology 200:155\u2013167. https:\/\/doi.org\/10.1016\/j.theriogenology.2023.02.011","journal-title":"Theriogenology"},{"key":"11114_CR6","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2024.1357594","volume":"15","author":"Y Gao","year":"2024","unstructured":"Gao Y, Wang Z, Long Y et al (2024) Unveiling the roles of Sertoli cells lineage differentiation in reproductive development and disorders: a review. Front Endocrinol 15:1357594. https:\/\/doi.org\/10.3389\/fendo.2024.1357594","journal-title":"Front Endocrinol"},{"key":"11114_CR7","doi-asserted-by":"publisher","DOI":"10.3390\/cells10081959","volume":"10","author":"MD Hade","year":"2021","unstructured":"Hade MD, Suire CN, Suo Z (2021) Mesenchymal stem cell-derived exosomes: applications in regenerative medicine. Cells 10:1959. https:\/\/doi.org\/10.3390\/cells10081959","journal-title":"Cells"},{"key":"11114_CR8","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-60351-5","volume":"10","author":"W Han","year":"2020","unstructured":"Han W, Yang F, Wu Z et al (2020) Inner Mongolian Cashmere Goat Secondary Follicle Development Regulation Research Based on mRNA-miRNA co-analysis. Sci Rep 10:4519. https:\/\/doi.org\/10.1038\/s41598-020-60351-5","journal-title":"Sci Rep"},{"key":"11114_CR9","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2022.118960","volume":"301","author":"J Huang","year":"2022","unstructured":"Huang J, Ren H, Chen A et al (2022) Perfluorooctane sulfonate induces suppression of testosterone biosynthesis via Sertoli cell-derived exosomal\/miR-9-3p downregulating StAR expression in Leydig cells. Environ Pollut 301:118960. https:\/\/doi.org\/10.1016\/j.envpol.2022.118960","journal-title":"Environ Pollut"},{"key":"11114_CR10","doi-asserted-by":"publisher","DOI":"10.3390\/ijms251910569","volume":"25","author":"J Huang","year":"2024","unstructured":"Huang J, Li S, Yang Y et al (2024) GPX5-enriched exosomes improve sperm quality and fertilization ability. Int J Mol Sci 25:10569. https:\/\/doi.org\/10.3390\/ijms251910569","journal-title":"Int J Mol Sci"},{"key":"11114_CR11","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-12819-5","volume":"15","author":"\u0130 \u0130nan\u00e7","year":"2025","unstructured":"\u0130nan\u00e7 \u0130, Bender O, Atalay A, Erdemli E (2025) The relationship between sperm secretion and the Hippo signaling pathway in the rat seminiferous tubules. Sci Rep 15:28204. https:\/\/doi.org\/10.1038\/s41598-025-12819-5","journal-title":"Sci Rep"},{"key":"11114_CR12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2023.103833","volume":"156","author":"Y Jahanbani","year":"2023","unstructured":"Jahanbani Y, Beiranvand T, Mamaghani PY et al (2023) Exosome- based technologies as a platform for diagnosis and treatment of male and female infertility-related diseases. J Reprod Immunol 156:103833. https:\/\/doi.org\/10.1016\/j.jri.2023.103833","journal-title":"J Reprod Immunol"},{"key":"11114_CR13","doi-asserted-by":"publisher","first-page":"1188","DOI":"10.1089\/dna.2019.4830","volume":"38","author":"Z Ji","year":"2019","unstructured":"Ji Z, He R, Chao T et al (2019) Chi-miR-143-3p promotes apoptosis of mammary gland epithelial cells from dairy goats by targeting Ndfip1. DNA Cell Biol 38:1188\u20131196. https:\/\/doi.org\/10.1089\/dna.2019.4830","journal-title":"DNA Cell Biol"},{"key":"11114_CR14","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.12710","volume":"9","author":"H Jie","year":"2021","unstructured":"Jie H, Xu Z, Gao J et al (2021) Differential expression profiles of microRNAs in musk gland of unmated and mated forest musk deer ( Moschus berezovskii ). PeerJ 9:e12710. https:\/\/doi.org\/10.7717\/peerj.12710","journal-title":"PeerJ"},{"key":"11114_CR15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1521230113","author":"J Kowal","year":"2016","unstructured":"Kowal J, Arras G, Colombo M et al (2016) Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. https:\/\/doi.org\/10.1073\/pnas.1521230113","journal-title":"Proc Natl Acad Sci U S A"},{"key":"11114_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2024.102369","volume":"35","author":"J Liang","year":"2024","unstructured":"Liang J, Mei J, Chen D et al (2024) The role of Sertoli cell-derived miR-143-3p in male fertility declines with age. Mol Ther Nucleic Acids 35:102369. https:\/\/doi.org\/10.1016\/j.omtn.2024.102369","journal-title":"Mol Ther Nucleic Acids"},{"key":"11114_CR17","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1007\/s12265-017-9784-7","volume":"12","author":"X Liu","year":"2019","unstructured":"Liu X, Yuan W, Yang L et al (2019) MiRNA profiling of exosomes from spontaneous hypertensive rats using Next-Generation sequencing. J Cardiovasc Transl Res 12:75\u201383. https:\/\/doi.org\/10.1007\/s12265-017-9784-7","journal-title":"J Cardiovasc Transl Res"},{"key":"11114_CR18","doi-asserted-by":"publisher","first-page":"7123","DOI":"10.1007\/s00216-022-04253-7","volume":"414","author":"W Liu","year":"2022","unstructured":"Liu W, Ma Z, Kang X (2022) Current status and outlook of advances in exosome isolation. Anal Bioanal Chem 414:7123\u20137141. https:\/\/doi.org\/10.1007\/s00216-022-04253-7","journal-title":"Anal Bioanal Chem"},{"key":"11114_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2025.149571","volume":"962","author":"A Liu","year":"2025","unstructured":"Liu A, Gao X-H, Mao Y et al (2025) Esketamine mitigates lung injury in COPD rat models under mechanical ventilation: an RNA-sequencing and bioinformatics analysis of serum exosome miRNA profiles. Gene 962:149571. https:\/\/doi.org\/10.1016\/j.gene.2025.149571","journal-title":"Gene"},{"key":"11114_CR20","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.70106","volume":"240","author":"S Mehdinejadiani","year":"2025","unstructured":"Mehdinejadiani S, Azad N, Dehghan Z et al (2025) Role of seminal exosomes in reproduction. J Cell Physiol 240:e70106. https:\/\/doi.org\/10.1002\/jcp.70106","journal-title":"J Cell Physiol"},{"key":"11114_CR21","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1038\/s41419-019-1782-z","volume":"10","author":"F-D Ni","year":"2019","unstructured":"Ni F-D, Hao S-L, Yang W-X (2019) Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis. Cell Death Dis 10:541. https:\/\/doi.org\/10.1038\/s41419-019-1782-z","journal-title":"Cell Death Dis"},{"key":"11114_CR22","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.semcdb.2021.06.016","volume":"121","author":"L O\u2019Donnell","year":"2022","unstructured":"O\u2019Donnell L, Smith LB, Rebourcet D (2022) Sertoli cells as key drivers of testis function. Semin Cell Dev Biol 121:2\u20139. https:\/\/doi.org\/10.1016\/j.semcdb.2021.06.016","journal-title":"Semin Cell Dev Biol"},{"key":"11114_CR23","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13112433","volume":"13","author":"W Peng","year":"2025","unstructured":"Peng W, Liu H, Wang K et al (2025) Effects of rumen-protected methionine and lysine on the fecal microbiota of Leizhou goats. Microorganisms 13:2433. https:\/\/doi.org\/10.3390\/microorganisms13112433","journal-title":"Microorganisms"},{"key":"11114_CR24","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.897196","volume":"13","author":"VA Ruthig","year":"2022","unstructured":"Ruthig VA, Lamb DJ (2022) Updates in Sertoli cell-mediated signaling during spermatogenesis and advances in restoring Sertoli cell function. Front Endocrinol 13:897196. https:\/\/doi.org\/10.3389\/fendo.2022.897196","journal-title":"Front Endocrinol"},{"key":"11114_CR25","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2023.152046","volume":"125","author":"H Saito","year":"2023","unstructured":"Saito H, Yokota S, Kitajima S (2023) Immunohistochemical analysis of the vimentin filaments in Sertoli cells is a powerful tool for the prediction of spermatogenic dysfunction. Acta Histochem 125:152046. https:\/\/doi.org\/10.1016\/j.acthis.2023.152046","journal-title":"Acta Histochem"},{"key":"11114_CR26","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1007\/s00467-017-3816-z","volume":"34","author":"A St\u00e5hl","year":"2019","unstructured":"St\u00e5hl A, Johansson K, Mossberg M et al (2019) Exosomes and microvesicles in normal physiology, pathophysiology, and renal diseases. Pediatr Nephrol 34:11\u201330. https:\/\/doi.org\/10.1007\/s00467-017-3816-z","journal-title":"Pediatr Nephrol"},{"key":"11114_CR27","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.31202","volume":"239","author":"H Tian","year":"2024","unstructured":"Tian H, Wang X, Li X et al (2024) Regulation of spermatogonial stem cell differentiation by Sertoli cells-derived exosomes through paracrine and autocrine signaling. J Cell Physiol 239:e31202. https:\/\/doi.org\/10.1002\/jcp.31202","journal-title":"J Cell Physiol"},{"key":"11114_CR28","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-025-11282-w","volume":"26","author":"K Wang","year":"2025","unstructured":"Wang K, Xu M, Han X et al (2025) Transcriptome analysis of muscle atrophy in Leizhou black goats: identification of key genes and insights into limb-girdle muscular dystrophy. BMC Genomics 26:80. https:\/\/doi.org\/10.1186\/s12864-025-11282-w","journal-title":"BMC Genomics"},{"key":"11114_CR29","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.913502","volume":"13","author":"RL Washburn","year":"2022","unstructured":"Washburn RL, Hibler T, Kaur G, Dufour JM (2022) Sertoli cell immune regulation: a double-edged sword. Front Immunol 13:913502. https:\/\/doi.org\/10.3389\/fimmu.2022.913502","journal-title":"Front Immunol"},{"key":"11114_CR30","unstructured":"Wong WJ, Khan YS (2025) Histology, Sertoli Cell. StatPearls. StatPearls Publishing, Treasure Island (FL)"},{"key":"11114_CR31","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2025.1538990","volume":"12","author":"B Xi","year":"2025","unstructured":"Xi B, An X, Yue Y et al (2025) Identification and profiling of microRNAs during sheep\u2019s testicular development. Front Vet Sci 12:1538990. https:\/\/doi.org\/10.3389\/fvets.2025.1538990","journal-title":"Front Vet Sci"},{"key":"11114_CR32","doi-asserted-by":"publisher","DOI":"10.3390\/cells13040313","volume":"13","author":"Z Xiao","year":"2024","unstructured":"Xiao Z, Liang J, Huang R et al (2024) Inhibition of miR-143-3p restores blood\u2013testis barrier function and ameliorates Sertoli cell senescence. Cells 13:313. https:\/\/doi.org\/10.3390\/cells13040313","journal-title":"Cells"},{"key":"11114_CR33","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2024.1456410","volume":"15","author":"Y Xiao","year":"2025","unstructured":"Xiao Y, Zhang J, Guan Y et al (2025) Research progress on Sertoli cell secretion during spermatogenesis. Front Endocrinol 15:1456410. https:\/\/doi.org\/10.3389\/fendo.2024.1456410","journal-title":"Front Endocrinol"},{"key":"11114_CR34","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/s00232-022-00260-y","volume":"256","author":"W-M Xu","year":"2023","unstructured":"Xu W-M, Li A, Chen J-J, Sun E-J (2023) Research development on exosome separation technology. J Membr Biol 256:25\u201334. https:\/\/doi.org\/10.1007\/s00232-022-00260-y","journal-title":"J Membr Biol"},{"key":"11114_CR35","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1089\/cell.2016.0019","volume":"18","author":"Y Zhang","year":"2016","unstructured":"Zhang Y, Wang Y, Zuo Q et al (2016) Effects of the transforming growth factor beta signaling pathway on the differentiation of chicken embryonic stem cells into male germ cells. Cell Reprogram 18:401\u2013410. https:\/\/doi.org\/10.1089\/cell.2016.0019","journal-title":"Cell Reprogram"},{"key":"11114_CR36","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/j.theriogenology.2018.12.029","volume":"126","author":"G-M Zhang","year":"2019","unstructured":"Zhang G-M, Zhang T-T, An S-Y et al (2019) Expression of Hippo signaling pathway components in Hu sheep male reproductive tract and spermatozoa. Theriogenology 126:239\u2013248. https:\/\/doi.org\/10.1016\/j.theriogenology.2018.12.029","journal-title":"Theriogenology"},{"key":"11114_CR37","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252313126","volume":"25","author":"Y Zhang","year":"2024","unstructured":"Zhang Y, Xiang Q-M, Mu C-K et al (2024) Functional study of PTSMAD4 in the spermatogenesis of the swimming crab Portunus trituberculatus. Int J Mol Sci 25:13126. https:\/\/doi.org\/10.3390\/ijms252313126","journal-title":"Int J Mol Sci"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11114-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11114-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11114-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T09:49:12Z","timestamp":1772876952000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11259-026-11114-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,19]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["11114"],"URL":"https:\/\/doi.org\/10.1007\/s11259-026-11114-6","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,19]]},"assertion":[{"value":"16 October 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 February 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 February 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experimental procedures were approved by the Laboratory Animal Ethics Committee of Guangdong Ocean University (approval no. GDOU-NXY-2024-039-413).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"162"}}