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Twelve adult rams were randomly assigned into two groups (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) in the non-breeding season. Rams in the PRP group received 0.2\u00a0ml\/per epididymis (150\u2013200\u2009\u00d7\u200910\u2076 platelets) of PRP every 15 days for a total of six injections, while control group received the same volume of saline. Semen samples were collected biweekly and pooled within each group before undergoing standard cryopreservation procedures. Post-thaw analyses included morphological, functional, biochemical, and molecular assessments. Compared to the control, intraepididimal PRP significantly increased hypo-osmotic swelling (HOS) response, total and progressive motility, rapid sperm percentage, and kinetic parameters (VCL- curvilinear velocity, VSL- straight-line velocity, VAP- average path velocity), while reducing static and acrosome-damaged sperm in thawed semen. Intraepididimal PRP also enhanced catalase activity, cholesterol and myristic acid (C14:0) concentrations and the levels of steroidogenic acute regulatory protein (StAR), 3\u03b2-hydroxysteroid dehydrogenase type 1 (HSD3\u03b21), cation channel of sperm (CatSper1), platelet-derived growth factor (PDGF), platelet-derived growth factor receptor (PDGFR), and reduced malondialdehyde level in thawed semen. Intraepididimal PRP significantly increased the mRNA presence of CatSper2, CatSper3, CatSper4, transient receptor potential melastatin 3 (TRPM3) and transient receptor potential vanilloid 5 (TRPV5) ion channels, oar-miR-3958-3p and oar-miR-125b, and decreased bta-miR-22-3p and rno-miR-494 in thawed semen. Additionally, intraepididimal PRP significantly upregulated the protein expressions of CatSper3, HSD3\u03b22 and PDGFA, and decreased protein expressions of vascular endothelial growth factor A (VEGFA) and transforming growth factor beta 1 (TGF\u03b21) in thawed semen. In conclusion, intraepididymal PRP administration improved cryoresistance in ram spermatozoa, likely due to its growth factors, lipids, and antioxidants. These effects enhanced post-thaw sperm quality by modulating oxidative stress, cholesterol, ion channels, microRNAs, and steroidogenic proteins, indicating PRP as a promising tool for improving semen cryotolerance in rams.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11130-6","type":"journal-article","created":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T09:52:48Z","timestamp":1772272368000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intraepididymal platelet-rich plasma improves semen cryoresistance via antioxidant, lipid and molecular modulation during the non-breeding season in rams"],"prefix":"10.1007","volume":"50","author":[{"given":"Serap Dayan","family":"Cinkara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nida","family":"Bad\u0131ll\u0131","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130brahim Halil","family":"G\u00fcng\u00f6r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asl\u0131han \u00c7ak\u0131r","family":"Cihangiro\u011flu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tutku Can","family":"Ac\u0131su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6rkem K\u0131rm\u0131z\u0131kaya","family":"\u00d6zmen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6zde","family":"Arkal\u0131","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa Sezer","family":"Bulan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet","family":"Tektemur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edip","family":"Toraman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u015eeyma \u00d6zer","family":"Kaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"S\u00f6nmez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyfettin","family":"G\u00fcr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdurrauf","family":"Y\u00fcce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6kke\u015f","family":"Y\u0131lmaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaffari","family":"T\u00fcrk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,28]]},"reference":[{"key":"11130_CR1","doi-asserted-by":"publisher","first-page":"113","DOI":"10.14715\/cmb\/2022a.68.4.14","volume":"68","author":"AK Abdulla","year":"2022","unstructured":"Abdulla AK, Rebai T, Al-Delemi DHJ (2022a) Protective effects of autologous platelet-rich plasma (PRP) on the outcome of cryopreservation in rabbit sperm. 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