{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T03:48:23Z","timestamp":1782100103547,"version":"3.54.5"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100020832","name":"Universidade De Passo Fundo","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100020832","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The equine parvovirus hepatitis (EqPV-H) was initially linked to fatal cases of equine serum hepatitis but, more recently, it has also been detected in sick or asymptomatic horses around the world, including in a limited number of horses in southeastern Brazil. In this study we aimed to assess the prevalence of EqPV-H DNA in the serum of healthy horses in the northern region of Rio Grande do Sul state, Brazil. For this purpose, we used 1000 serum samples collected from horses from 40 municipalities and organized them into 200 pools of five samples each, based on origin and age. Using a nested polymerase chain reaction (nPCR), we detected the EqPV-H DNA in 69 of the pools representing 34.5% of the total, with positive samples found in 26 (65%) of the municipalities. The prevalence did not differ significantly by age group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.52). The nucleotide and phylogenetic analysis conducted with the partial NS1 sequence obtained from five serum pools revealed a high degree of genetic conservation with previous worldwide isolates, indicating specific nucleotide variability present in strains from Brazil and Argentina but absent in strains from other countries. Our study provides the first evidence that EqPV-H is widespread in horses in the region and it should be considered in suggestive clinical cases of hepatitis.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11251-y","type":"journal-article","created":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T03:51:09Z","timestamp":1778298669000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular prevalence of equine parvovirus hepatitis in healthy horses from the Northern region of the state of Rio Grande do Sul, Brazil"],"prefix":"10.1007","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7612-630X","authenticated-orcid":false,"given":"Taline Scalco","family":"Picetti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4222-223X","authenticated-orcid":false,"given":"Andreza Soriano","family":"Figueiredo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4685-4840","authenticated-orcid":false,"given":"J\u00falia Trevizan","family":"Segalin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole Lopes","family":"Mostardeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"de Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3361-2420","authenticated-orcid":false,"given":"Rafael","family":"Frandoloso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7685-7401","authenticated-orcid":false,"given":"Luiz Carlos","family":"Kreutz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,9]]},"reference":[{"key":"11251_CR1","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1111\/evj.13444","volume":"54","author":"M Badenhorst","year":"2022","unstructured":"Badenhorst M, de Heus P, Auer A et al (2022) Active equine parvovirus-hepatitis infection is most frequently detected in Austrian horses of advanced age. Equine Vet J 54:379\u2013389. https:\/\/doi.org\/10.1111\/evj.13444","journal-title":"Equine Vet J"},{"key":"11251_CR2","doi-asserted-by":"publisher","unstructured":"Baird J, Tegtmeyer B, Arroyo L et al (2020) The association of Equine Parvovirus-Hepatitis (EqPV-H) with cases of non-biologic-associated Theiler\u2019s disease on a farm in Ontario, Canada. Vet Microbiol 242. https:\/\/doi.org\/10.1016\/j.vetmic.2019.108575","DOI":"10.1016\/j.vetmic.2019.108575"},{"key":"11251_CR5","doi-asserted-by":"publisher","first-page":"1188","DOI":"10.1101\/gr.849004","volume":"14","author":"GE Crooks","year":"2004","unstructured":"Crooks GE, Hon G, Chandonia JM, Brenner SE (2004) WebLogo: A sequence logo generator. Genome Res 14:1188\u20131190. https:\/\/doi.org\/10.1101\/gr.849004","journal-title":"Genome Res"},{"key":"11251_CR7","doi-asserted-by":"publisher","first-page":"303","DOI":"10.3201\/eid2402.171031","volume":"24","author":"TJ Divers","year":"2018","unstructured":"Divers TJ, Tennant BC, Kumar A et al (2018) New parvovirus associated with serum hepatitis in horses after inoculation of common biological product. Emerg Infect Dis 24:303\u2013310. https:\/\/doi.org\/10.3201\/eid2402.171031","journal-title":"Emerg Infect Dis"},{"key":"11251_CR8","doi-asserted-by":"publisher","unstructured":"Fortier C, El-Hage C, Normand C et al (2024) Detection of Equine Parvovirus-Hepatitis Virus and Equine Hepacivirus in Archived Sera from Horses in France and Australia. Viruses 16. https:\/\/doi.org\/10.3390\/v16060862","DOI":"10.3390\/v16060862"},{"key":"11251_CR3","unstructured":"IBGE (2023) Brazilian Institute of Geography and Statistics (2023) Produ\u00e7\u00e3o Agropecu\u00e1ria de Equinos no RS. In: Accessed 19 Nov 2025 https:\/\/www.ibge.gov.br\/explica\/producao-agropecuaria\/equinos\/rs"},{"key":"11251_CR9","doi-asserted-by":"crossref","unstructured":"Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol","DOI":"10.1007\/BF01731581"},{"key":"11251_CR10","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1093\/molbev\/msy096","volume":"35","author":"S Kumar","year":"2018","unstructured":"Kumar S, Stecher G, Li M et al (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547\u20131549. https:\/\/doi.org\/10.1093\/molbev\/msy096","journal-title":"Mol Biol Evol"},{"key":"11251_CR12","doi-asserted-by":"publisher","unstructured":"Lee SK, Park D, Lee I (2021) Molecular prevalence of equine parvovirus-hepatitis in the sera of clinically healthy horses in south korea. Vet Sci 8. https:\/\/doi.org\/10.3390\/vetsci8110282","DOI":"10.3390\/vetsci8110282"},{"key":"11251_CR13","doi-asserted-by":"publisher","unstructured":"Lu G, Sun L, Ou J et al (2018) Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China. Emerg Microbes Infect 7. https:\/\/doi.org\/10.1038\/s41426-018-0174-2","DOI":"10.1038\/s41426-018-0174-2"},{"key":"11251_CR14","doi-asserted-by":"publisher","unstructured":"Meister TL, Tegtmeyer B, Br\u00fcggemann Y et al (2019a) Characterization of equine parvovirus in thoroughbred breeding horses from Germany. https:\/\/doi.org\/10.3390\/v11100965. Viruses 11:","DOI":"10.3390\/v11100965"},{"key":"11251_CR15","doi-asserted-by":"publisher","unstructured":"Meister TL, Tegtmeyer B, Postel A et al (2019b) Equine parvovirus-hepatitis frequently detectable in commercial equine serum pools. https:\/\/doi.org\/10.3390\/v11050461. Viruses 11:","DOI":"10.3390\/v11050461"},{"key":"11251_CR6","doi-asserted-by":"publisher","first-page":"3022","DOI":"10.1111\/tbed.14226","volume":"69","author":"MV Moraes","year":"2022","unstructured":"Moraes MV, Salgado S, Godoi CRS et al (2022) TLOS, Equine parvovirus-hepatitis is detected in South America, Brazil. Transbound Emerg Dis 69:3022\u20133027. https:\/\/doi.org\/10.1111\/tbed.14226","journal-title":"Transbound Emerg Dis"},{"key":"11251_CR16","doi-asserted-by":"publisher","unstructured":"Olguin-Perglione C, Politzki R, Alvarez I, Ruiz V (2024) First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina. Vet J 306. https:\/\/doi.org\/10.1016\/j.tvjl.2024.106204","DOI":"10.1016\/j.tvjl.2024.106204"},{"key":"11251_CR17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.biologicals.2019.08.005","volume":"61","author":"WP Paim","year":"2019","unstructured":"Paim WP, Weber MN, Cibulski SP et al (2019) Characterization of the viral genomes present in commercial batches of horse serum obtained by high-throughput sequencing. Biologicals 61:1\u20137. https:\/\/doi.org\/10.1016\/j.biologicals.2019.08.005","journal-title":"Biologicals"},{"key":"11251_CR18","unstructured":"Papapetrou MA, Arroyo LG, Meister TL et al (2023) Prevalence of equine parvovirus-hepatitis in healthy broodmares in Ontario, Canada. Can J Veterinary Res 169\u2013175"},{"key":"11251_CR19","doi-asserted-by":"publisher","first-page":"886","DOI":"10.1111\/evj.13477","volume":"53","author":"AS Ramsauer","year":"2021","unstructured":"Ramsauer AS, Badenhorst M, Cavalleri JMV (2021) Equine parvovirus hepatitis. Equine Vet J 53:886\u2013894","journal-title":"Equine Vet J"},{"key":"11251_CR20","doi-asserted-by":"publisher","unstructured":"Reinecke B, Kl\u00f6hn M, Br\u00fcggemann Y et al (2021) Clinical course of infection and cross-species detection of equine parvovirus-hepatitis. Viruses 13. https:\/\/doi.org\/10.3390\/v13081454","DOI":"10.3390\/v13081454"},{"key":"11251_CR21","first-page":"7","volume":"5","author":"A Theiler","year":"1918","unstructured":"Theiler A (1918) Acute liver atrophy and parenchymatous hepatitis in horses. Rep Director Veterinary Res 5:7\u201399","journal-title":"Rep Director Veterinary Res"},{"key":"11251_CR22","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1080\/22221751.2020.1741326","volume":"9","author":"JE Tomlinson","year":"2020","unstructured":"Tomlinson JE, Jager M, Struzyna A et al (2020) Tropism, pathology, and transmission of equine parvovirus-hepatitis. Emerg Microbes Infect 9:651\u2013663. https:\/\/doi.org\/10.1080\/22221751.2020.1741326","journal-title":"Emerg Microbes Infect"},{"key":"11251_CR23","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1111\/jvim.15368","volume":"33","author":"JE Tomlinson","year":"2019","unstructured":"Tomlinson JE, Kapoor A, Kumar A et al (2019a) Viral testing of 18 consecutive cases of equine serum hepatitis: A prospective study (2014\u20132018). J Vet Intern Med 33:251\u2013257. https:\/\/doi.org\/10.1111\/jvim.15368","journal-title":"J Vet Intern Med"},{"key":"11251_CR24","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1111\/jvim.15362","volume":"33","author":"JE Tomlinson","year":"2019","unstructured":"Tomlinson JE, Tennant BC, Struzyna A et al (2019b) Viral testing of 10 cases of Theiler\u2019s disease and 37 in-contact horses in the absence of equine biologic product administration: A prospective study (2014\u20132018). J Vet Intern Med 33:258\u2013265. https:\/\/doi.org\/10.1111\/jvim.15362","journal-title":"J Vet Intern Med"},{"key":"11251_CR25","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1111\/evj.13254","volume":"52","author":"M Vengust","year":"2020","unstructured":"Vengust M, Jager MC, Zalig V et al (2020a) First report of equine parvovirus-hepatitis-associated Theiler\u2019s disease in Europe. Equine Vet J 52:841\u2013847. https:\/\/doi.org\/10.1111\/evj.13254","journal-title":"Equine Vet J"},{"key":"11251_CR26","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1111\/evj.13254","volume":"52","author":"M Vengust","year":"2020","unstructured":"Vengust M, Jager MC, Zalig V et al (2020b) First report of equine parvovirus-hepatitis-associated Theiler\u2019s disease in Europe. Equine Vet J 52:841\u2013847. https:\/\/doi.org\/10.1111\/evj.13254","journal-title":"Equine Vet J"},{"key":"11251_CR27","doi-asserted-by":"publisher","first-page":"1052","DOI":"10.1111\/tbed.13443","volume":"67","author":"J Xie","year":"2020","unstructured":"Xie J, Tong P, Zhang A et al (2020) An emerging equine parvovirus circulates in thoroughbred horses in north Xinjiang, China, 2018. Transbound Emerg Dis 67:1052\u20131056. https:\/\/doi.org\/10.1111\/tbed.13443","journal-title":"Transbound Emerg Dis"},{"key":"11251_CR30","doi-asserted-by":"publisher","unstructured":"Yoon J, Park T, Kim A et al (2021) First detection and genetic characterization of new equine parvovirus species circulating among horses in Korea. Vet Sci 8. https:\/\/doi.org\/10.3390\/vetsci8110268","DOI":"10.3390\/vetsci8110268"},{"key":"11251_CR28","doi-asserted-by":"publisher","first-page":"e3455","DOI":"10.1111\/tbed.14746","volume":"69","author":"J Yoon","year":"2022","unstructured":"Yoon J, Park T, Kim A et al (2022a) Molecular surveillance of equine parvovirus-hepatitis from oral, nasal, vaginal, and semen specimens collected from horses living in Korea. Transbound Emerg Dis 69:e3455\u2013e3461. https:\/\/doi.org\/10.1111\/tbed.14746","journal-title":"Transbound Emerg Dis"},{"key":"11251_CR29","doi-asserted-by":"publisher","first-page":"2735","DOI":"10.1111\/tbed.14425","volume":"69","author":"J Yoon","year":"2022","unstructured":"Yoon J, Park T, Kim A et al (2022b) First report of equine parvovirus-hepatitis and equine hepacivirus coinfection in horses in Korea. Transbound Emerg Dis 69:2735\u20132746. https:\/\/doi.org\/10.1111\/tbed.14425","journal-title":"Transbound Emerg Dis"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11251-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11251-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11251-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T03:03:11Z","timestamp":1782097391000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11259-026-11251-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,9]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["11251"],"URL":"https:\/\/doi.org\/10.1007\/s11259-026-11251-y","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,9]]},"assertion":[{"value":"13 December 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 April 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 May 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The serum samples included in this investigation were provided by the Veterinary Diagnostic Laboratory (VDL) at the Universidade de Passo Fundo. Field veterinarians collected blood samples and submitted them to the VDL for the diagnosis of equine infectious anemia virus and glanders, in accordance with the National Program of Equine Health (document SDA\/MAA 45\/2004, published in the Official Journal of the Union (DOU) on 07\/07\/2004, and document n\u00ba 6 from 16\/01\/2018, published in the Official Journal (DOU) of the Union on 17\/01\/2018), which mandates that all equids transported and participating in any collective activity must be free of antibodies to both diseases. Upon the completion of the mandatory storage duration of 90 days, the samples may be discharged. This investigation exclusively utilized sera samples designated for discharging. Consequently, since none of the samples were specifically collected for this study, no animals were utilized, and the information provided with the samples included only the geographical origin and the animals\u2019 age and sex, the study did not require approval from the University Commission of Animal Ethics.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"311"}}