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This prospective study evaluated short-term changes in serological, inflammatory, and serum protein electrophoretic parameters during first-line therapy with meglumine antimoniate plus allopurinol. Eighteen dogs with confirmed clinical disease were enrolled at diagnosis, and fifteen dogs completing all follow-up visits (Days 0, 7, 14, 21, and 29) were included. Infection was confirmed by compatible clinical and\/or laboratory abnormalities, high anti-\n                    <jats:italic>Leishmania<\/jats:italic>\n                    antibody levels, and successful parasite isolation. Anti-\n                    <jats:italic>Leishmania<\/jats:italic>\n                    antibodies were quantified using in-house single-dilution and two-fold serial dilution ELISA; circulating immune complexes, C-reactive protein, a complete blood count, a biochemical profile, and serum protein electrophoresis were also assessed. Both ELISA methods showed a significant decline in antibody levels during treatment, with serial dilution ELISA showing greater discrimination among highly positive samples. C-reactive protein concentrations decreased significantly from diagnosis to Day 29, indicating reduced systemic inflammation. Serum protein electrophoresis showed significant increases in albumin and the albumin:globulin ratio, along with a significant decrease in gamma globulins. Circulating immune complexes concentrations remained stable. These findings support quantitative serology, C-reactive protein, and serum protein electrophoresis as useful tools for early monitoring of therapeutic response in clinical canine leishmaniosis.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11275-4","type":"journal-article","created":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T03:20:15Z","timestamp":1778901615000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Changes in serological, inflammatory, and serum protein electrophoretic markers during ongoing meglumine antimoniate plus allopurinol therapy in canine leishmaniosis"],"prefix":"10.1007","volume":"50","author":[{"given":"Jacobo","family":"Giner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Eugenia","family":"Lebrero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Quilez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Guallar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1lex","family":"G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Marteles-Arag\u00fc\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Villanueva-Saz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,16]]},"reference":[{"key":"11275_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0065-308X(04)57001-X","volume":"57","author":"J Alvar","year":"2004","unstructured":"Alvar J, Ca\u00f1avate C, Molina R, Moreno J, Nieto J (2004) Canine leishmaniasis. 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