{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T03:48:16Z","timestamp":1782100096719,"version":"3.54.5"},"reference-count":12,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100014417","name":"Veterinary Research Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100014417","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Taylorella equigenitalis<\/jats:italic>\n                    , the causative agent of contagious equine metritis, is a fastidious bacterium with limited tolerance to extra-host conditions; however, quantitative data on its growth dynamics and survival outside the host remain scarce. This study evaluated the growth of\n                    <jats:italic>T. equigenitalis<\/jats:italic>\n                    under aerobic and microaerophilic conditions in liquid medium and its survival in wet and dry straw-based bedding under controlled laboratory conditions.\n                  <\/jats:p>\n                  <jats:p>\n                    Growth was assessed at 20\u00a0\u00b0C and 37\u00a0\u00b0C using real-time optical density monitoring and viable cell counts. No proliferation was observed at 20\u00a0\u00b0C, whereas incubation at 37\u00a0\u00b0C resulted in measurable growth after 25\u201333\u00a0h. Viable cell counts were higher after incubation under aerobic conditions than under microaerophilic conditions, with a mean difference of 0.45 log\n                    <jats:sub>10<\/jats:sub>\n                    . Bacterial survival in straw-based bedding declined progressively over seven days, with significantly higher counts maintained under wet conditions compared to dry conditions. Viable cells remained detectable until the end of the experiment, particularly in wet bedding.\n                  <\/jats:p>\n                  <jats:p>\n                    The results provide quantitative data on the growth characteristics of\n                    <jats:italic>T. equigenitalis<\/jats:italic>\n                    and underscore the importance of appropriate hygiene and biosecurity measures in equine breeding facilities.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11278-1","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T04:31:52Z","timestamp":1779165112000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Growth kinetics of Taylorella equigenitalis and its survival in straw-based bedding"],"prefix":"10.1007","volume":"50","author":[{"given":"Martina","family":"Florianova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslava","family":"Krzyzankova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristyna","family":"Korena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3054-4746","authenticated-orcid":false,"given":"Helena","family":"Juricova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"11278_CR1","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1186\/1471-2180-14-69","volume":"19","author":"J Allombert","year":"2014","unstructured":"Allombert J, Vianney A, Laugier C, Petry S, H\u00e9bert L (2014) Survival of taylorellae in the environmental amoeba Acanthamoeba castellanii. BMC Microbiol Mar 19:14:69. https:\/\/doi.org\/10.1186\/1471-2180-14-69","journal-title":"BMC Microbiol Mar"},{"key":"11278_CR2","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.jevs.2019.03.217","volume":"78","author":"M Delerue","year":"2019","unstructured":"Delerue M, Breuil M-F, Duquesne F, Bayon-Auboyer M-H, Amenna-Bernard N, Petry S (2019) Acute Endometritis due to Taylorella equigenitalis Transmission by Insemination of Cryopreserved Stallion Semen. J Equine Vet Sci 78:10\u201313. https:\/\/doi.org\/10.1016\/j.jevs.2019.03.217","journal-title":"J Equine Vet Sci"},{"key":"11278_CR3","doi-asserted-by":"publisher","first-page":"105629","DOI":"10.1016\/j.tvjl.2021.105629","volume":"270","author":"F Duquesne","year":"2021","unstructured":"Duquesne F, Breuil MF, Hans A, Petry S (2021) Preservation of viable Taylorella equigenitalis in different commercially available transport systems. Vet J Apr 270:105629. https:\/\/doi.org\/10.1016\/j.tvjl.2021.105629","journal-title":"Vet J Apr"},{"issue":"9","key":"11278_CR4","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1136\/vr.103.9.187","volume":"103","author":"DS Fernie","year":"1978","unstructured":"Fernie DS (1978) Growth of the contagious equine metritis organism in a liquid medium. Vet Rec 103(9):187\u2013188. https:\/\/doi.org\/10.1136\/vr.103.9.187","journal-title":"Vet Rec"},{"issue":"2","key":"11278_CR5","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1111\/evj.14121","volume":"57","author":"M Grabatin","year":"2025","unstructured":"Grabatin M, Fux R, Zablotski Y, Goehring LS, Witte TS (2025) Taylorella equigenitalis in Icelandic intact males compared with other horse breeds using natural cover. Equine Vet J 57(2):441\u2013448. https:\/\/doi.org\/10.1111\/evj.14121","journal-title":"Equine Vet J"},{"key":"11278_CR6","doi-asserted-by":"publisher","first-page":"0378","DOI":"10.1016\/j.vetmic.2025.110839","volume":"312","author":"M Harpke","year":"2026","unstructured":"Harpke M, Brangsch H, Melzer F (2026) Genomic insights into the epidemiology of Contagious Equine Metritis in Germany. Vet Microbiol Volume 312:0378\u20131135. https:\/\/doi.org\/10.1016\/j.vetmic.2025.110839","journal-title":"Vet Microbiol Volume"},{"issue":"1","key":"11278_CR7","doi-asserted-by":"publisher","first-page":"e29953","DOI":"10.1371\/journal.pone.0029953","volume":"7","author":"L H\u00e9bert","year":"2012","unstructured":"H\u00e9bert L, Moumen B, Pons N, Duquesne F, Breuil MF, Goux D, Batto JM, Laugier C, Renault P, Petry S (2012) Genomic characterization of the Taylorella genus. PLoS ONE 7(1):e29953. https:\/\/doi.org\/10.1371\/journal.pone.0029953","journal-title":"PLoS ONE"},{"issue":"1","key":"11278_CR8","doi-asserted-by":"publisher","first-page":"e0315946","DOI":"10.1371\/journal.pone.0315946","volume":"20","author":"M Hrala","year":"2025","unstructured":"Hrala M, Andrla P, Bos\u00e1k J, Fedrov\u00e1 P, Mugutdinov A, Karp\u00ed\u0161kov\u00e1 R, Nedbalcov\u00e1 K, Raichov\u00e1 J, Faldyna M, Ho\u0159\u00edn P, \u0160majs D (2025) Whole genome sequences of nine Taylorella equigenitalis strains isolated in the Czech Republic between 1982\u20132021: Molecular dating suggests a common ancestor at the time of Roman Empire. PLoS ONE 20(1):e0315946. https:\/\/doi.org\/10.1371\/journal.pone.0315946","journal-title":"PLoS ONE"},{"issue":"1\u20132","key":"11278_CR10","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.vetmic.2012.12.021","volume":"167","author":"ML Schulman","year":"2013","unstructured":"Schulman ML, May CE, Keys B, Guthrie AJ (2013) Contagious equine metritis: Artificial reproduction changes the epidemiologic paradigm. Vet Microbiol 167(1\u20132):2\u20138. https:\/\/doi.org\/10.1016\/j.vetmic.2012.12.021","journal-title":"Vet Microbiol"},{"key":"11278_CR9","doi-asserted-by":"publisher","first-page":"105247","DOI":"10.1016\/j.jevs.2024.105247","volume":"144","author":"V Solbach","year":"2025","unstructured":"Solbach V, Grabatin M, Zablotski Y, Fux R, Zerbe H, Witte TS (2025) Prevalence of Taylorella equigenitalis in Icelandic mares and geldings in Southern Germany and Austria. J Equine Vet Sci 144:105247. https:\/\/doi.org\/10.1016\/j.jevs.2024.105247","journal-title":"J Equine Vet Sci"},{"key":"11278_CR11","doi-asserted-by":"publisher","unstructured":"Timoney PJ (2011) Horse species symposium: contagious equine metritis: an insidious threat to the horse breeding industry in the United States. J Anim Sci 89(5):1552-60. https:\/\/doi.org\/10.2527\/jas.2010-3368","DOI":"10.2527\/jas.2010-3368"},{"key":"11278_CR12","unstructured":"World Organisation for Animal Health (WOAH) (2022) Part 3, Chap. 3.6.2, Contagious equine metritis. In: Manual of diagnostic tests and vaccines for terrestrial animals, 12th edition https:\/\/www.woah.org\/fileadmin\/Home\/eng\/Health_standards\/tahm\/3.06.02_CEM.pdf"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11278-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11278-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11278-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T03:02:23Z","timestamp":1782097343000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11259-026-11278-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,19]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["11278"],"URL":"https:\/\/doi.org\/10.1007\/s11259-026-11278-1","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,19]]},"assertion":[{"value":"4 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 May 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study did not require official or institutional ethical approval because the\n                      T. equigenitalis\n                      TE31 isolate was isolated during mandatory routine veterinary screening.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"331"}}