{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T17:02:14Z","timestamp":1789837334264,"version":"4.0.1"},"reference-count":71,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1007\/s11259-026-11311-3","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:11:46Z","timestamp":1780546306000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Leveraging the antibacterial and antibiofilm activities of Cymbopogon flexuosus\u00a0essential oil against multidrug-resistant bacteria recovered from avian colibacillosis and bovine mastitis: in vitro and molecular docking insights"],"prefix":"10.1007","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0595-2591","authenticated-orcid":false,"given":"Sana","family":"Dhaouadi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3617-5705","authenticated-orcid":false,"given":"Oussema","family":"Khamessi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8436-2353","authenticated-orcid":false,"given":"Roua Ben","family":"Dassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3521-7002","authenticated-orcid":false,"given":"Abdennacer","family":"Boulila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4161-9569","authenticated-orcid":false,"given":"Ramzi Boubaker","family":"Elandoulsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9028-0717","authenticated-orcid":false,"given":"Rim Driouich","family":"Chaouachi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,4]]},"reference":[{"key":"11311_CR1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1265027","volume":"13","author":"MI Abd El-Hamid","year":"2023","unstructured":"Abd El-Hamid MI, El-Tarabili RM, Bahnass MM, Alshahrani MA, Saif A, Alwutayd KM, Safhi FA, Mansour AT, Alblwi NAN, Ghoneim MM, Elmaaty AA, Al-harthi HF, Bendary MM (2023) Partnering essential oils with antibiotics: Proven therapies against bovine Staphylococcus aureus mastitis. Front Cell Infect Microbiol 13:1265027. https:\/\/doi.org\/10.3389\/fcimb.2023.1265027","journal-title":"Front Cell Infect Microbiol"},{"issue":"20","key":"11311_CR2","doi-asserted-by":"publisher","DOI":"10.3390\/plants13202893","volume":"13","author":"F Achim\u00f3n","year":"2024","unstructured":"Achim\u00f3n F, Peschiutta ML, Brito VD, Ulla SB, Pizzolitto RP (2024) Sulcatone as a plant-derived volatile organic compound for the control of the maize weevil and its associated phytopathogenic fungi in stored maize. Plants (Basel) 13(20):2893. https:\/\/doi.org\/10.3390\/plants13202893","journal-title":"Plants (Basel)"},{"key":"11311_CR3","volume-title":"Identification of essential oil components by gas chromatography\/mass spectrometry","author":"RP Adams","year":"2017","unstructured":"Adams RP (2017) Identification of essential oil components by gas chromatography\/mass spectrometry. Texensis Publishing, Gruver"},{"issue":"5","key":"11311_CR4","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1111\/j.1365-2672.2012.05418.x","volume":"113","author":"E Adukwu","year":"2012","unstructured":"Adukwu E, Allen SC, Phillips CA (2012) The anti-biofilm activity of lemongrass (Cymbopogon flexuosus) and grapefruit (Citrus paradisi) essential oils against five strains of Staphylococcus aureus. J Appl Microbiol 113(5):1217\u20131227. https:\/\/doi.org\/10.1111\/j.1365-2672.2012.05418.x","journal-title":"J Appl Microbiol"},{"key":"11311_CR5","doi-asserted-by":"publisher","DOI":"10.2306\/scienceasia1513-1874.2021.093","author":"J Aiemsaard","year":"2021","unstructured":"Aiemsaard J, Kamollerd C, Seubsasana S, Thongkham E, Vonghataipaisarn P (2021) Lemongrass essential oil enhances antibacterial activity of cephalexin against Staphylococcus pseudintermedius isolated from dogs with superficial pyoderma. ScienceAsia. https:\/\/doi.org\/10.2306\/scienceasia1513-1874.2021.093","journal-title":"ScienceAsia"},{"key":"11311_CR6","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.cep.2017.12.012","volume":"124","author":"MA Balti","year":"2018","unstructured":"Balti MA, Hadrich B, Kriaa K, Kechaou N (2018) Lab-scale extraction of essential oils from Tunisian lemongrass (Cymbopogon flexuosus). Chem Eng Process Process Intensif 124:164\u2013173. https:\/\/doi.org\/10.1016\/j.cep.2017.12.012","journal-title":"Chem Eng Process Process Intensif"},{"issue":"4","key":"11311_CR7","doi-asserted-by":"publisher","first-page":"3989","DOI":"10.3390\/molecules17043989","volume":"17","author":"IHN Bassol\u00e9","year":"2012","unstructured":"Bassol\u00e9 IHN, Juliani HR (2012) Essential oils in combination and their antimicrobial properties. Molecules 17(4):3989\u20134006. https:\/\/doi.org\/10.3390\/molecules17043989","journal-title":"Molecules"},{"issue":"5","key":"11311_CR8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010790","volume":"5","author":"KE Beenken","year":"2010","unstructured":"Beenken KE, Mrak LN, Griffin LM, Zielinska AK, Shaw LN, Rice KC, Horswill AR, Bayles KW, Smeltzer MS (2010) Epistatic relationships between sarA and agr in Staphylococcus aureus biofilm formation. PLoS One 5(5):e10790. https:\/\/doi.org\/10.1371\/journal.pone.0010790","journal-title":"PLoS One"},{"issue":"19","key":"11311_CR9","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30193873","volume":"30","author":"BB Belete","year":"2025","unstructured":"Belete BB, Ozkan J, Kalaiselvan P, Willcox M (2025) Clinical potential of essential oils: Cytotoxicity, selectivity Index, and efficacy for combating Gram-Positive ESKAPE pathogens. Molecules 30(19):3873. https:\/\/doi.org\/10.3390\/molecules30193873","journal-title":"Molecules"},{"key":"11311_CR10","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2021.101543","volume":"8","author":"P Bellio","year":"2021","unstructured":"Bellio P, Fagnani L, Ly N, Celenza G (2021) New and simplified method for drug combination studies by checkerboard assay. MethodsX 8:101543. https:\/\/doi.org\/10.1016\/j.mex.2021.101543","journal-title":"MethodsX"},{"issue":"1","key":"11311_CR11","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1093\/nar\/28.1.235","volume":"28","author":"HM Berman","year":"2000","unstructured":"Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN, Bourne PE (2000) The protein data bank. Nucleic Acids Res 28(1):235\u2013242. https:\/\/doi.org\/10.1093\/nar\/28.1.235","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"11311_CR12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000052","volume":"4","author":"BR Boles","year":"2008","unstructured":"Boles BR, Horswill AR (2008) Agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS Pathog 4(4):e1000052. https:\/\/doi.org\/10.1371\/journal.ppat.1000052","journal-title":"PLoS Pathog"},{"key":"11311_CR13","doi-asserted-by":"publisher","first-page":"35","DOI":"10.3389\/fvets2015.00035","volume":"2","author":"CA O\u2019Bryan","year":"2015","unstructured":"O\u2019Bryan CA, Pendleton SJ, Crandall PG, Ricke SC (2015) Potential of plant essential oils and their components in animal agriculture\u2013in vitro studies on antibacterial mode of action. Front Vet Sci 2:35. https:\/\/doi.org\/10.3389\/fvets2015.00035","journal-title":"Front Vet Sci"},{"issue":"3","key":"11311_CR14","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/j.ijfoodmicro.2004.03.022","volume":"94","author":"S Burt","year":"2004","unstructured":"Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods\u2013a review. Int J Food Microbiol 94(3):223\u2013253. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2004.03.022","journal-title":"Int J Food Microbiol"},{"key":"11311_CR15","doi-asserted-by":"publisher","first-page":"3228","DOI":"10.20546\/ijcmas.2018.704.366","volume":"7","author":"S Chakraborty","year":"2018","unstructured":"Chakraborty S, Dutta TK, De A, Das M, Ghosh S (2018) Impact of bacterial biofilm in veterinary medicine: an overview. Int J Curr Microbiol Appl Sci 7:3228\u20133239. https:\/\/doi.org\/10.20546\/ijcmas.2018.704.366","journal-title":"Int J Curr Microbiol Appl Sci"},{"issue":"1","key":"11311_CR16","first-page":"11","volume":"12","author":"A Chauhan","year":"2023","unstructured":"Chauhan A, Sharma AD, Kaur I (2023) Bio-active components of Cymbopogon martinii essential oil as therapeutics targeting bacterial penicillin-binding-proteins (PBPs): aroma profile, in silico-docking, pharmacokinetics and wet-lab validation. J BioSci Biotechnol 12(1):11\u201331","journal-title":"J BioSci Biotechnol"},{"issue":"11","key":"11311_CR17","doi-asserted-by":"publisher","first-page":"1699","DOI":"10.5713\/ajas.20.0156","volume":"33","author":"WN Cheng","year":"2020","unstructured":"Cheng WN, Han SG (2020) Bovine mastitis: Risk factors, therapeutic strategies, and alternative treatments\u2014A review. Asian-Australas J Anim Sci 33(11):1699. https:\/\/doi.org\/10.5713\/ajas.20.0156","journal-title":"Asian-Australas J Anim Sci"},{"key":"11311_CR18","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2026) Performance standards for antimicrobial susceptibility testing. 36th ed. CLSI supplement M100. Wayne, PA: CLSI. https:\/\/clsi.org\/shop\/standards\/m100\/ (accessed January 30, 2026)"},{"key":"11311_CR19","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1590\/0034-737X201966050006","volume":"66","author":"KAD Costa","year":"2019","unstructured":"Costa KAD, Moura R, Millezi AF (2019) Antimicrobial and antibiofilm activity of Cymbopogon flexuosus essential oil microemulsions. Rev Ceres 66:372\u2013379. https:\/\/doi.org\/10.1590\/0034-737X201966050006","journal-title":"Rev Ceres"},{"key":"11311_CR20","doi-asserted-by":"publisher","unstructured":"Dallakyan S, Olson AJ (2014) Small-molecule library screening by docking with PyRx. In: Hempel, J., Williams, C., Hong, C. (eds) Chemical Biology. Methods Mol Biol 1263. https:\/\/doi.org\/10.1007\/978-1-4939-2269-7_19","DOI":"10.1007\/978-1-4939-2269-7_19"},{"key":"11311_CR21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2019.105826","volume":"55","author":"S Dhaouadi","year":"2020","unstructured":"Dhaouadi S, Soufi L, Campanile F, Dhaouadi F, Sociale M, Lazzaro L, Elandoulsi RB (2020a) Prevalence of meticillin-resistant and -susceptible coagulase-negative staphylococci with the first detection of the mecC gene among cows, humans and manure in Tunisia. Int J Antimicrob Agents 55:105826. https:\/\/doi.org\/10.1016\/j.ijantimicag.2019.105826","journal-title":"Int J Antimicrob Agents"},{"key":"11311_CR22","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1016\/j.jgar.2020b.07.015","volume":"22","author":"S Dhaouadi","year":"2020","unstructured":"Dhaouadi S, Soufi L, Hamza A, Fedida D, Zied C, Awadhi E, Landolsi RB (2020bb) Co-occurrence of mcr-1 mediated colistin resistance and \u03b2-lactamase-encoding genes in multidrug-resistant Escherichia coli from broiler chickens with colibacillosis in Tunisia. J Glob Antimicrob Resist 22:538\u2013545. https:\/\/doi.org\/10.1016\/j.jgar.2020b.07.015","journal-title":"J Glob Antimicrob Resist"},{"key":"11311_CR23","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1016\/j.jgar.2022.03.020","volume":"31","author":"S Dhaouadi","year":"2022","unstructured":"Dhaouadi S, Bouchami O, Soufi L, Dhaouadi F, Chaari S, Bouglita W, Miragaia M (2022) Frequent dissemination and carriage of an SCCmec-mecC hybrid in methicillin-resistant Mammaliicoccus sciuri in farm animals from Tunisia. J Glob Antimicrob Resist 31:228\u2013235. https:\/\/doi.org\/10.1016\/j.jgar.2022.03.020","journal-title":"J Glob Antimicrob Resist"},{"key":"11311_CR24","doi-asserted-by":"publisher","unstructured":"Dhaouadi S, Romdhani A, Bouglita W, Chedli S, Chaari S, Soufi L, Cherif A, Mnif W, Abbassi MS, Elandoulsi RB (2023) High biofilm-forming ability and clonal dissemination among colistin-resistant Escherichia coli isolates recovered from cows with mastitis, diarrheic calves, and chickens with colibacillosis in Tunisia. Life. https:\/\/doi.org\/10.3390\/life13020299","DOI":"10.3390\/life13020299"},{"key":"11311_CR25","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-024-03981-6","volume":"82","author":"S Dhaouadi","year":"2025","unstructured":"Dhaouadi S, Ghorbel SKB, Bouglita W, Chaari S, Dhifi W, Khrouf R, Cherif A, Elandoulsi RB (2025) The potency of Cupressus sempervirens and Eucalyptus globulus essential oils against antibiotic-resistant Escherichia coli and Mammaliicoccus sciuri from diseased animals in Tunisia. Curr Microbiol 82:14. https:\/\/doi.org\/10.1007\/s00284-024-03981-6","journal-title":"Curr Microbiol"},{"issue":"3","key":"11311_CR26","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-026-11138-y","volume":"50","author":"S Dhaouadi","year":"2026","unstructured":"Dhaouadi S, Titouche Y, Dhaouadi F, Akkou M, Elandoulsi RB (2026) Mammaliicoccus sciuri as a sentinel for antimicrobial resistance and virulence: genomic epidemiology, transmission dynamics, and control in the one health era. Vet Res Commun 50(3):217. https:\/\/doi.org\/10.1007\/s11259-026-11138-y","journal-title":"Vet Res Commun"},{"issue":"12","key":"11311_CR27","doi-asserted-by":"publisher","first-page":"4124","DOI":"10.1128\/jcm.01121-14","volume":"52","author":"CD Doern","year":"2014","unstructured":"Doern CD (2014) When does 2 plus 2 equal 5? A review of antimicrobial synergy testing. J Clin Microbiol 52(12):4124\u20134128. https:\/\/doi.org\/10.1128\/jcm.01121-14","journal-title":"J Clin Microbiol"},{"issue":"8","key":"11311_CR28","doi-asserted-by":"publisher","first-page":"3891","DOI":"10.1021\/acs.jcim.1c00203","volume":"61","author":"J Eberhardt","year":"2021","unstructured":"Eberhardt J, Santos-Martins D, Tillack AF, Forli S (2021) AutoDock Vina 1.2.0: New Docking Methods, Expanded Force Field, and Python Bindings. J Chem Inf Model 61(8):3891\u20133898. https:\/\/doi.org\/10.1021\/acs.jcim.1c00203","journal-title":"J Chem Inf Model"},{"issue":"W1","key":"11311_CR29","doi-asserted-by":"publisher","first-page":"W478","DOI":"10.1093\/nar\/gkaf377","volume":"53","author":"C Ehrt","year":"2025","unstructured":"Ehrt C, Schulze T, Graef J, Diedrich K, Pletzer-Zelgert J, Rarey M (2025) ProteinsPlus: a publicly available resource for protein structure mining. Nucleic Acids Res 53(W1):W478\u2013W484. https:\/\/doi.org\/10.1093\/nar\/gkaf377","journal-title":"Nucleic Acids Res"},{"key":"11311_CR30","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01971","volume":"10","author":"D Espadinha","year":"2019","unstructured":"Espadinha D, Sobral RG, Mendes CI, M\u00e9ric G, Sheppard SK, Carri\u00e7o JA, de Lencastre H, Miragaia M (2019) Distinct phenotypic and genomic signatures underlie contrasting pathogenic potential of Staphylococcus epidermidis clonal lineages. Front Microbiol 10:1971. https:\/\/doi.org\/10.3389\/fmicb.2019.01971","journal-title":"Front Microbiol"},{"issue":"W1","key":"11311_CR31","doi-asserted-by":"publisher","first-page":"W337","DOI":"10.1093\/nar\/gkx333","volume":"45","author":"R F\u00e4hrrolfes","year":"2017","unstructured":"F\u00e4hrrolfes R, Bietz S, Flachsenberg F, Meyder A, Nittinger E, Otto T, Volkamer A, Rarey M (2017) Proteins Plus: a web portal for structure analysis of macromolecules. Nucleic Acids Res 45(W1):W337\u2013W343. https:\/\/doi.org\/10.1093\/nar\/gkx333","journal-title":"Nucleic Acids Res"},{"key":"11311_CR32","unstructured":"Food and Drug Administration (2015a) Title 21, Vol. 3. http:\/\/www.accessdata.fda.gov\/scripts\/cdrh\/cfdocs\/cfcfr\/cfrsearch.cfm?fr=182.20. Accessed October 2015"},{"issue":"8","key":"11311_CR33","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1136\/jcp.42.8.872","volume":"42","author":"DJ Freeman","year":"1989","unstructured":"Freeman DJ, Falkiner FR, Keane CT (1989) New method for detecting slime production by coagulase negative staphylococci. J Clin Pathol 42(8):872. https:\/\/doi.org\/10.1136\/jcp.42.8.872","journal-title":"J Clin Pathol"},{"key":"11311_CR34","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.603858","volume":"10","author":"S Gao","year":"2020","unstructured":"Gao S, Liu G, Li J, Chen J, Li L, Li Z, Zhang X, Zhang S, Thorne RF, Zhang S (2020) Antimicrobial activity of lemongrass essential oil (Cymbopogon flexuosus) and its active component citral against dual-species biofilms of Staphylococcus aureus and Candida species. Front Cell Infect Microbiol 10:603858. https:\/\/doi.org\/10.3389\/fcimb.2020.603858","journal-title":"Front Cell Infect Microbiol"},{"issue":"4","key":"11311_CR35","doi-asserted-by":"publisher","first-page":"5130","DOI":"10.3390\/s130405130","volume":"13","author":"B Gray","year":"2013","unstructured":"Gray B, Hall P, Gresham H (2013) Targeting agr-and agr-like quorum sensing systems for development of common therapeutics to treat multiple gram-positive bacterial infections. Sensors 13(4):5130\u20135166. https:\/\/doi.org\/10.3390\/s130405130","journal-title":"Sensors"},{"issue":"15","key":"11311_CR36","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnv118","volume":"362","author":"R Guabiraba","year":"2015","unstructured":"Guabiraba R, Schouler C (2015) Avian colibacillosis: still many black holes. FEMS Microbiol Lett 362(15):fnv118. https:\/\/doi.org\/10.1093\/femsle\/fnv118","journal-title":"FEMS Microbiol Lett"},{"key":"11311_CR37","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1128\/jb.158.2.513-516.1984","volume":"158","author":"BJ Hartman","year":"1984","unstructured":"Hartman BJ, Tomasz A (1984) Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus. J Bacteriol 158:513\u2013516. https:\/\/doi.org\/10.1128\/jb.158.2.513-516.1984","journal-title":"J Bacteriol"},{"key":"11311_CR38","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2021.108775","volume":"135","author":"S Kang","year":"2022","unstructured":"Kang S, Li X, Xing Z, Liu X, Bai X, Yang Y, Guo D, Xia X, Zhang C, Shi C (2022) Antibacterial effect of citral on Yersinia enterocolitica and its mechanism. Food Control 135:108775. https:\/\/doi.org\/10.1016\/j.foodcont.2021.108775","journal-title":"Food Control"},{"key":"11311_CR39","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.ijmm.2006.01.042","volume":"296","author":"KF Kong","year":"2006","unstructured":"Kong KF, Vuong C, Otto M (2006) Staphylococcus quorum sensing in biofilm formation and infection. Int J Med Microbiol 296:133\u2013139. https:\/\/doi.org\/10.1016\/j.ijmm.2006.01.042","journal-title":"Int J Med Microbiol"},{"key":"11311_CR40","doi-asserted-by":"publisher","unstructured":"Kuai YH, Law JWF, Ong YS, Letchumanan V, Lee LH, Tan LTH (2024) Role of SarA in Staphylococcus aureus: a virulence target for therapeutic strategies. Prog Microb Mol Biol 7(1). https:\/\/doi.org\/10.36877\/pmmb.a0000444","DOI":"10.36877\/pmmb.a0000444"},{"key":"11311_CR41","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1016\/j.lwt.2016.03.023","volume":"73","author":"JS Lee","year":"2016","unstructured":"Lee JS, Bae YM, Han A, Lee SY (2016) Development of Congo red broth method for the detection of biofilm-forming or slime-producing Staphylococcus spp. LWT 73:707\u2013714. https:\/\/doi.org\/10.1016\/j.lwt.2016.03.023","journal-title":"LWT"},{"issue":"9","key":"11311_CR42","doi-asserted-by":"publisher","first-page":"3057","DOI":"10.1016\/j.foodres.2011.07.030","volume":"44","author":"F Lv","year":"2011","unstructured":"Lv F, Liang H, Yuan Q, Li C (2011) In vitro antimicrobial effects and mechanism of action of selected plant essential oil combinations against four food-related microorganisms. Food Res Int 44(9):3057\u20133064. https:\/\/doi.org\/10.1016\/j.foodres.2011.07.030","journal-title":"Food Res Int"},{"key":"11311_CR43","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1111\/j.1574-6976.2006.00024.x","volume":"30","author":"P Macheboeuf","year":"2006","unstructured":"Macheboeuf P, Contreras-Martel C, Job V, Dideberg O, Dessen A (2006) Penicillin binding proteins: Key players in bacterial cell cycle and drug resistance processes. FEMS Microbiol Rev 30:673\u2013691. https:\/\/doi.org\/10.1111\/j.1574-6976.2006.00024.x","journal-title":"FEMS Microbiol Rev"},{"key":"11311_CR44","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/8843954","volume":"2024","author":"P Manu","year":"2024","unstructured":"Manu P, Nketia PB, Osei-Poku P, Kwarteng A (2024) Computational mutagenesis and inhibition of Staphylococcus aureus AgrA LytTR domain using phenazine scaffolds: insight from a biophysical study. BioMed Res Int 2024:8843954. https:\/\/doi.org\/10.1155\/2024\/8843954","journal-title":"BioMed Res Int"},{"issue":"1","key":"11311_CR45","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/s13620-022-00209-6","volume":"75","author":"SJ More","year":"2022","unstructured":"More SJ, McCoy F, McAloon CI (2022) The new Veterinary Medicines Regulation: rising to the challenge. Ir Vet J 75(1):2. https:\/\/doi.org\/10.1186\/s13620-022-00209-6","journal-title":"Ir Vet J"},{"issue":"7","key":"11311_CR46","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1016\/S1995-7645(10)60129-0","volume":"3","author":"MI Naik","year":"2010","unstructured":"Naik MI, Fomda BA, Jaykumar E, Bhat JA (2010) Antibacterial activity of lemongrass (Cymbopogon citratus) oil against some selected pathogenic bacteria. Asian Pac J Trop Med 3(7):535\u2013538. https:\/\/doi.org\/10.1016\/S1995-7645(10)60129-0","journal-title":"Asian Pac J Trop Med"},{"issue":"2","key":"11311_CR47","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10020330","volume":"10","author":"R Nehme","year":"2021","unstructured":"Nehme R, Andr\u00e9s S, Pereira RB, Ben Jemaa M, Bouhallab S, Ceciliani F, L\u00f3pez S, Rahali FZ, Ksouri R, Pereira DM (2021) Essential oils in livestock: from health to food quality. Antioxidants 10(2):330. https:\/\/doi.org\/10.3390\/antiox10020330","journal-title":"Antioxidants"},{"issue":"1","key":"11311_CR48","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/jac\/dkg301","volume":"52","author":"FC Odds","year":"2003","unstructured":"Odds FC (2003) Synergy, antagonism, and what the chequerboard puts between them. J Antimicrob Chemother 52(1):1. https:\/\/doi.org\/10.1093\/jac\/dkg301","journal-title":"J Antimicrob Chemother"},{"issue":"1","key":"11311_CR49","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-95971-y","volume":"11","author":"HBM Oliveira","year":"2021","unstructured":"Oliveira HBM, Selis N, Sampaio BA, J\u00fanior MNS, de Carvalho SP, de Almeida JB, Almeida PP, da Silva IBS, Oliveira CNT, Brito TLS (2021) Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus. Sci Rep 11(1):16482. https:\/\/doi.org\/10.1038\/s41598-021-95971-y","journal-title":"Sci Rep"},{"key":"11311_CR50","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10010109","author":"M Pajohi Alamoti","year":"2022","unstructured":"Pajohi Alamoti M, Bazargani-Gilani B, Mahmoudi R, Reale A, Pakbin B, Di Renzo T, Kaboudari A (2022) Essential oils from indigenous Iranian plants: a natural weapon vs. multidrug-resistant Escherichia coli. Microorganisms. https:\/\/doi.org\/10.3390\/microorganisms10010109","journal-title":"Microorganisms"},{"key":"11311_CR51","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119306","volume":"273","author":"B Palaniappan","year":"2021","unstructured":"Palaniappan B, Solomon AP (2021) Targeting AgrA quorum sensing regulator by bumetanide attenuates virulence in Staphylococcus aureus\u2013a drug repurposing approach. Life Sci 273:119306. https:\/\/doi.org\/10.1016\/j.lfs.2021.119306","journal-title":"Life Sci"},{"key":"11311_CR52","doi-asserted-by":"publisher","first-page":"2612","DOI":"10.1128\/aac.49.7.2612-2617.2005","volume":"49","author":"RK Pettit","year":"2005","unstructured":"Pettit RK, Weber CA, Kean MJ, Hoffmann H, Pettit GR, Tan R, Franks KS, Horton ML (2005) Microplate Alamar blue assay for Staphylococcus epidermidis biofilm susceptibility testing. Antimicrob Agents Chemother 49:2612\u20132617. https:\/\/doi.org\/10.1128\/aac.49.7.2612-2617.2005","journal-title":"Antimicrob Agents Chemother"},{"issue":"24","key":"11311_CR53","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26247542","volume":"26","author":"B Piasecki","year":"2021","unstructured":"Piasecki B, Biernasiuk A, Skiba A, Skalicka-Wo\u017aniak K, Ludwiczuk A (2021) Composition, anti-MRSA activity and toxicity of essential oils from Cymbopogon species. Molecules 26(24):7542. https:\/\/doi.org\/10.3390\/molecules26247542","journal-title":"Molecules"},{"key":"11311_CR54","doi-asserted-by":"publisher","DOI":"10.1039\/d1ra00488c","author":"FA Qais","year":"2021","unstructured":"Qais FA, Ahmad I, Altaf M, Manoharadas S, Al-Rayes BF, Abuhasil MS, Almaroaie YA (2021) Biofabricated silver nanoparticles exhibit broad-spectrum antibiofilm and antiquorum sensing activity against Gram-negative bacteria. RSC Adv. https:\/\/doi.org\/10.1039\/d1ra00488c","journal-title":"RSC Adv"},{"issue":"6","key":"11311_CR55","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29061343","volume":"29","author":"Q Qian","year":"2024","unstructured":"Qian Q, Zhuo Z, Peng Y, Xu D (2024) Chemical composition variation in essential oil and their correlation with climate factors in Chinese prickly ash peels (Zanthoxylum armatum DC.) from different habitats. Molecules 29(6):1343. https:\/\/doi.org\/10.3390\/molecules29061343","journal-title":"Molecules"},{"issue":"1","key":"11311_CR56","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202400966","volume":"22","author":"N Raghuvanshi","year":"2025","unstructured":"Raghuvanshi N, Gupta B, Joshi V, Bisht SS, Manikpuri M, Shukla K, Khokhar D, Singh N, Ghosh KK (2025) Influence of essential oil composition on antioxidant and antibacterial activities of three cultivars of Cymbopogon flexuosus: in vitro and in silico study. Chem Biodivers 22(1):e202400966. https:\/\/doi.org\/10.1002\/cbdv.202400966","journal-title":"Chem Biodivers"},{"issue":"8","key":"11311_CR57","doi-asserted-by":"publisher","first-page":"1527","DOI":"10.3390\/microorganisms10081527","volume":"10","author":"C Salinas","year":"2022","unstructured":"Salinas C, Florent\u00edn G, Rodr\u00edguez F, Alvarenga N, Guill\u00e9n R (2022) Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion. Microorganisms 10(8):1527. https:\/\/doi.org\/10.3390\/microorganisms10081527","journal-title":"Microorganisms"},{"key":"11311_CR58","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1093\/jac\/dkl013","volume":"57","author":"JAT Sandoe","year":"2006","unstructured":"Sandoe JAT, Wysome J, West AP, Heritage J, Wilcox MH (2006) Measurement of ampicillin, vancomycin, linezolid and gentamicin activity against enterococcal biofilms. J Antimicrob Chemother 57:767\u2013770. https:\/\/doi.org\/10.1093\/jac\/dkl013","journal-title":"J Antimicrob Chemother"},{"key":"11311_CR59","first-page":"8","volume":"1","author":"L Schrodinger","year":"2015","unstructured":"Schrodinger L (2015) The PyMOL molecular graphics system. Version 1:8","journal-title":"Version"},{"issue":"1","key":"11311_CR60","doi-asserted-by":"publisher","DOI":"10.1038\/srep43243","volume":"7","author":"C Shi","year":"2017","unstructured":"Shi C, Sun Y, Liu Z, Guo D, Sun H, Sun Z, Chen S, Zhang W, Wen Q, Peng X (2017) Inhibition of Cronobacter sakazakii virulence factors by citral. Sci Rep 7(1):43243. https:\/\/doi.org\/10.1038\/srep43243","journal-title":"Sci Rep"},{"key":"11311_CR61","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2017-0011","author":"M Shoham","year":"2017","unstructured":"Shoham M, Greenberg M (2017) Preventing the spread of infectious diseases: antivirulents versus antibiotics. Future Microbiol. https:\/\/doi.org\/10.2217\/fmb-2017-0011","journal-title":"Future Microbiol"},{"issue":"2","key":"11311_CR62","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/j.burns.2016.08.032","volume":"43","author":"M Sienkiewicz","year":"2017","unstructured":"Sienkiewicz M, \u0141ysakowska M, Kowalczyk E, Szyma\u0144ska G, Kochan E, Krukowska J, Olszewski J, Bli\u017aniewska HZ (2017) The ability of selected plant essential oils to enhance the action of recommended antibiotics against pathogenic wound bacteria. Burns 43(2):310\u2013317. https:\/\/doi.org\/10.1016\/j.burns.2016.08.032","journal-title":"Burns"},{"key":"11311_CR63","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-020-03112-1","volume":"20","author":"G Singh","year":"2020","unstructured":"Singh G, Katoch M (2020) Antimicrobial activities and mechanism of action of Cymbopogon khasianus (Munro ex Hackel) Bor essential oil. BMC Complement Med Ther 20:331. https:\/\/doi.org\/10.1186\/s12906-020-03112-1","journal-title":"BMC Complement Med Ther"},{"issue":"6","key":"11311_CR64","doi-asserted-by":"publisher","first-page":"1928","DOI":"10.1111\/j.1365-2672.2009.04597.x","volume":"108","author":"M Somolinos","year":"2010","unstructured":"Somolinos M, Garc\u00eda D, Cond\u00f3n S, Mackey B, Pag\u00e1n R (2010) Inactivation of Escherichia coli by citral. J Appl Microbiol 108(6):1928\u20131939. https:\/\/doi.org\/10.1111\/j.1365-2672.2009.04597.x","journal-title":"J Appl Microbiol"},{"issue":"1","key":"11311_CR65","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1080\/1828051X.2024.2304042","volume":"23","author":"C Spadini","year":"2024","unstructured":"Spadini C, Iannarelli M, Carrillo Heredero AM, Montanaro SL, Mezzasalma N, Simoni M, Bertini S, Righi F, Cabassi CS (2024) Stability of the antimicrobial activity of selected essential oils and nature identical compounds and their interaction with Tween 20 against reference bacterial strains of zootechnical interest. Ital J Anim Sci 23(1):189\u2013199. https:\/\/doi.org\/10.1080\/1828051X.2024.2304042","journal-title":"Ital J Anim Sci"},{"key":"11311_CR66","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1111\/j.1600-0463.2007.apm_630.x","volume":"115","author":"S Stepanovic","year":"2007","unstructured":"Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I, Ruzicka F (2007) Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS 115:891\u2013899. https:\/\/doi.org\/10.1111\/j.1600-0463.2007.apm_630.x","journal-title":"APMIS"},{"issue":"01","key":"11311_CR67","doi-asserted-by":"publisher","first-page":"84","DOI":"10.7324\/JABB.2020.80114","volume":"8","author":"H Toukourou","year":"2020","unstructured":"Toukourou H, Sounouvou H, Catteau L, Toukourou F, Van Bambeke F, Gbaguidi F, Quetin-Leclercq J (2020) Cymbopogon giganteus Chiov. essential oil: direct effects or activity in combination with antibiotics against multi-drug resistant bacteria. J Appl Biol Biotechnol 8(01):84\u201389. https:\/\/doi.org\/10.7324\/JABB.2020.80114","journal-title":"J Appl Biol Biotechnol"},{"key":"11311_CR68","doi-asserted-by":"publisher","DOI":"10.1016\/j.jciso.2023.100096","volume":"12","author":"R Vaidyanathan","year":"2023","unstructured":"Vaidyanathan R, Murugan Sreedevi S, Ravichandran K, Vinod SM, Hari Krishnan Y, Babu LK, Parthiban PS, Basker L, Perumal T, Rajaraman V, Arumugam G, Rajendran K, Mahalingam V (2023) Molecular docking approach on the binding stability of derivatives of phenolic acids (DPAs) with Human Serum Albumin (HSA): hydrogen-bonding versus hydrophobic interactions or combined influences? JCIS Open 12:100096. https:\/\/doi.org\/10.1016\/j.jciso.2023.100096","journal-title":"JCIS Open"},{"key":"11311_CR69","volume-title":"Clinical and Laboratory Standards Institute Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically Standard, Approval CDM-A","author":"MP Weinstein","year":"2018","unstructured":"Weinstein MP, Patel JB, Burnhman CA, Zimmer BL (2018) Clinical and Laboratory Standards Institute Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically Standard, Approval CDM-A, vol 38. Clinical and Laboratory Standards Institute, Wayne, PA, USA"},{"key":"11311_CR70","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24112130","volume":"24","author":"K Wi\u0144ska","year":"2019","unstructured":"Wi\u0144ska K, M\u0105czka W, \u0141yczko J, Grabarczyk M, Czubaszek A, Szumny A (2019) Essential oils as antimicrobial agents\u2014myth or real alternative? Molecules 24:2130. https:\/\/doi.org\/10.3390\/molecules24112130","journal-title":"Molecules"},{"issue":"6","key":"11311_CR71","doi-asserted-by":"publisher","first-page":"1838","DOI":"10.1128\/jb.186.6.1838-1850.2004","volume":"186","author":"JM Yarwood","year":"2004","unstructured":"Yarwood JM, Bartels DJ, Volper EM, Greenberg EP (2004) Quorum sensing in Staphylococcus aureus biofilms. J Bacteriol 186(6):1838\u20131850. https:\/\/doi.org\/10.1128\/jb.186.6.1838-1850.2004","journal-title":"J Bacteriol"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11311-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11311-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11311-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T16:01:30Z","timestamp":1789833690000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11259-026-11311-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,4]]},"references-count":71,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["11311"],"URL":"https:\/\/doi.org\/10.1007\/s11259-026-11311-3","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,4]]},"assertion":[{"value":"12 February 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 June 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The strains used were obtained from other studies, following approval by the CEEA-ENMV at the National School of Veterinary Medicine of Sidi Thabet, Ariana, Tunisia, reference number 01. 2019\/ISBST.","order":1,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","label":"Consent for publication","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"366"}}