{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:21:14Z","timestamp":1792783274675,"version":"4.5.0"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universidade Federal De Pelotas","id":[{"id":"https:\/\/ror.org\/05msy9z54","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Bovine tuberculosis (bTB) is a chronic infectious disease caused by\n                    <jats:italic>Mycobacterium bovis<\/jats:italic>\n                    , which has major veterinary, economic, and zoonotic relevance. This study aimed to characterize the histopathological patterns of bTB lesions and their association with molecular detection in cattle slaughtered in Southern Brazil. Thirty-six tissue samples with lesions suggestive of bTB were collected during municipal inspection in Pelotas, Rio Grande do Sul. Polymerase chain reaction (PCR) targeting the 16\u00a0S rRNA gene was used to detect\n                    <jats:italic>Mycobacterium<\/jats:italic>\n                    species. DNA in 35\/36 samples (97.2%), whereas Ziehl\u2013Neelsen (ZN) staining revealed acid-fast bacilli in 23\/36 cases (61.1%), a statistically significant difference (McNemar\u2019s exact test,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0018). Grossly, the lesions ranged from caseous nodules to purulent and cavitary forms. Histologically, two main morphological patterns were identified: classical granulomatous lesions with complete fibrous encapsulation (19\/36, 52.8%) and disorganized granulomatous inflammation lacking complete encapsulation (17\/36, 47.2%). Multinucleated giant cells were present in all ZN-positive cases, whereas neutrophilic infiltration was significantly more frequent in disorganized lesions than in classical granulomas (Fisher\u2019s exact test,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0002), suggesting active inflammation and impaired granuloma containment. PCR showed higher sensitivity than ZN staining, detecting mycobacterial DNA even in lesions with a low bacillary load. As the assay targeted the 16\u00a0S rRNA gene, results were reported as\n                    <jats:italic>Mycobacterium<\/jats:italic>\n                    spp., and species-specific methods would be required for the definitive identification of\n                    <jats:italic>M. bovis<\/jats:italic>\n                    . These findings reinforce the diagnostic value of combining histopathology and molecular methods and emphasize the importance of recognizing atypical lesion patterns for the accurate detection of bovine tuberculosis in slaughterhouse surveillance systems.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11347-5","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T04:59:00Z","timestamp":1781240340000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Histopathological patterns of bovine tuberculosis in cattle slaughtered in Southern Brazil: correlation with molecular detection of Mycobacterium spp"],"prefix":"10.1007","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7568-8143","authenticated-orcid":false,"given":"Taina dos Santos","family":"Alberti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2920-3456","authenticated-orcid":false,"given":"Kau\u00ea Rodriguez","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1933-2331","authenticated-orcid":false,"given":"Virgilio Zoppi","family":"Lemos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5593-3110","authenticated-orcid":false,"given":"Clairton","family":"Marcolongo-Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5541-2956","authenticated-orcid":false,"given":"Marta Santos","family":"de Moraes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0929-1791","authenticated-orcid":false,"given":"Margarida Buss","family":"Raffi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0145-0321","authenticated-orcid":false,"given":"Rodrigo Casquero","family":"Cunha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3033-9876","authenticated-orcid":false,"given":"Eliza Simone Vi\u00e9gas","family":"Sallis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"issue":"2","key":"11347_CR1","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1590\/1678-5150-PVB-6406","volume":"40","author":"TS Alberti","year":"2020","unstructured":"Alberti TS, Bruhn FRP, Zamboni R, Venancio FR, Scheid HV, Raffi MB et al (2020) Epidemiological analysis of bovine tuberculosis in the southern region of Rio Grande do Sul from 2000 to 2015. Pesq Vet Bras 40(2):77\u201381. https:\/\/doi.org\/10.1590\/1678-5150-PVB-6406","journal-title":"Pesq Vet Bras"},{"key":"11347_CR2","doi-asserted-by":"publisher","first-page":"e07538","DOI":"10.1590\/1678-5150-PVB-7538","volume":"45","author":"TS Alberti","year":"2025","unstructured":"Alberti TS, Clairton Marcolongo-Pereira, Venancio FR, Scheid HV, Souza CG, Martins KR et al (2025) Epidemiological and pathological dynamics of tuberculosis in Jersey cattle in southeastern Rio Grande do Sul. Pesq Vet Bras 45:e07538. https:\/\/doi.org\/10.1590\/1678-5150-PVB-7538","journal-title":"Pesq Vet Bras"},{"key":"11347_CR3","first-page":"443","volume-title":"Doen\u00e7as de ruminantes e equinos","author":"AGC Dalto","year":"2023","unstructured":"Dalto AGC, Pavarini SP (2023) Tuberculose. In: Riet-Correa F, Schild AL, Lemos R, Borges JR, Mendon\u00e7a FS, Machado M (eds) Doen\u00e7as de ruminantes e equinos, 4th edn. MedVet, S\u00e3o Paulo, pp 443\u2013455","edition":"4"},{"key":"11347_CR4","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1590\/1678-5150-PVB-6294","volume":"40","author":"LL Dametto","year":"2020","unstructured":"Dametto LL, dos Santos ED, Santos LR, Dickel EL (2020) Bovine tuberculosis: diagnosis in dairy cattle through the association of analyzes. Pesq Vet Bras 40:12\u201316. https:\/\/doi.org\/10.1590\/1678-5150-PVB-6294","journal-title":"Pesq Vet Bras"},{"issue":"11","key":"11347_CR5","doi-asserted-by":"publisher","first-page":"1138","DOI":"10.1590\/S0100-736X2012001100011","volume":"32","author":"LV Furlanetto","year":"2012","unstructured":"Furlanetto LV, Eust\u00e1quio E, Adam C, C\u00e9sar R, Silva FGS, Silva JT et al (2012) Uso de m\u00e9todos complementares na inspe\u00e7\u00e3o post mortem de carca\u00e7as com suspeita de tuberculose bovina. Pesq Vet Bras 32(11):1138\u20131144. https:\/\/doi.org\/10.1590\/S0100-736X2012001100011","journal-title":"Pesq Vet Bras"},{"key":"11347_CR6","doi-asserted-by":"publisher","first-page":"e984293","DOI":"10.3389\/fimmu.2022.984293","volume":"13","author":"E Gaffney","year":"2022","unstructured":"Gaffney E, Murphy D, Walsh A, Connolly S, Basdeo SA, Keane J et al (2022) Defining the role of neutrophils in the lung during infection: Implications for tuberculosis disease. Front Immunol 13:e984293. https:\/\/doi.org\/10.3389\/fimmu.2022.984293","journal-title":"Front Immunol"},{"issue":"6","key":"11347_CR7","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1177\/0300985813485099","volume":"50","author":"KN Gibson-Corley","year":"2013","unstructured":"Gibson-Corley KN, Olivier AK, Meyerholz DK (2013) Principles for valid histopathologic scoring in research. Vet Pathol 50(6):1007\u20131015. https:\/\/doi.org\/10.1177\/0300985813485099","journal-title":"Vet Pathol"},{"key":"11347_CR8","doi-asserted-by":"publisher","first-page":"645802","DOI":"10.1155\/2014\/645802","volume":"2014","author":"T Jagielski","year":"2014","unstructured":"Jagielski T, Van Soolingen D, Zwolska Z (2014) Current methods in the molecular typing of Mycobacterium tuberculosis and other mycobacteria. Biomed Res Int 2014:645802. https:\/\/doi.org\/10.1155\/2014\/645802","journal-title":"Biomed Res Int"},{"issue":"15","key":"11347_CR9","doi-asserted-by":"publisher","first-page":"1098","DOI":"10.1056\/NEJMoa011110","volume":"345","author":"J Keane","year":"2001","unstructured":"Keane J, Gershon S, Wise RP, MirabileLevens E, Kasznica J, Schwieterman WD, Siegel JN, Braun MM (2001) Tuberculosis associated with infliximab, a tumor necrosis factor alpha-neutralizing agent. N Engl J Med 345(15):1098\u20131104. https:\/\/doi.org\/10.1056\/NEJMoa011110","journal-title":"N Engl J Med"},{"issue":"5","key":"11347_CR10","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1177\/03009858241231606","volume":"61","author":"F Larenas-Mu\u00f1oz","year":"2024","unstructured":"Larenas-Mu\u00f1oz F, Hamed MG, Ruedas-Torres I et al (2024) Macrophage polarization in lymph node granulomas from cattle and pigs naturally infected with Mycobacterium tuberculosis complex. Vet Pathol 61(5):792\u2013802. https:\/\/doi.org\/10.1177\/03009858241231606","journal-title":"Vet Pathol"},{"issue":"2","key":"11347_CR11","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1002\/art.27271","volume":"62","author":"PL Lin","year":"2010","unstructured":"Lin PL, Myers A, Smith L, Bigbee C, Bigbee M, Fuhrman C, Grieser H, Chiosea I, Voitene NN, Capuano SV, Klein E, Flynn JL (2010) Tumor necrosis factor neutralization results in disseminated disease in acute and latent Mycobacterium tuberculosis infection with normal granuloma structure in a cynomolgus macaque model. Arthritis Rheum 62(2):340\u2013350. https:\/\/doi.org\/10.1002\/art.27271","journal-title":"Arthritis Rheum"},{"key":"11347_CR12","doi-asserted-by":"publisher","first-page":"8619307","DOI":"10.1155\/2017\/8619307","volume":"2017","author":"IV Lyadova","year":"2017","unstructured":"Lyadova IV (2017) Neutrophils in tuberculosis: heterogeneity shapes the way? Mediators Inflamm 2017:8619307. https:\/\/doi.org\/10.1155\/2017\/8619307","journal-title":"Mediators Inflamm"},{"issue":"1","key":"11347_CR13","doi-asserted-by":"publisher","first-page":"e53884","DOI":"10.1371\/journal.pone.0053884","volume":"8","author":"\u00c1 Menin","year":"2013","unstructured":"Menin \u00c1, Fleith R, Reck C, Marlow M, Fernandes P, Pilati C, B\u00e1fica A (2013) Asymptomatic cattle naturally infected with Mycobacterium bovis present exacerbated tissue pathology and bacterial dissemination. PLoS ONE 8(1):e53884. https:\/\/doi.org\/10.1371\/journal.pone.0053884","journal-title":"PLoS ONE"},{"issue":"6","key":"11347_CR14","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1354\/vp.44-6-863","volume":"44","author":"MV Palmer","year":"2007","unstructured":"Palmer MV, Waters WR, Thacker TC (2007) Lesion development and immunohistochemical changes in granulomas from cattle experimentally infected with Mycobacterium bovis. Vet Pathol 44(6):863\u2013874. https:\/\/doi.org\/10.1354\/vp.44-6-863","journal-title":"Vet Pathol"},{"issue":"4","key":"11347_CR15","doi-asserted-by":"publisher","first-page":"544","DOI":"10.1177\/0300985819833454","volume":"56","author":"MV Palmer","year":"2019","unstructured":"Palmer MV, Wiarda J, Kanipe C, Thacker TC (2019) Early pulmonary lesions in cattle infected via aerosolized Mycobacterium bovis. Vet Pathol 56(4):544\u2013554. https:\/\/doi.org\/10.1177\/0300985819833454","journal-title":"Vet Pathol"},{"issue":"1","key":"11347_CR16","doi-asserted-by":"publisher","first-page":"61","DOI":"10.3390\/pathogens11010061","volume":"11","author":"MV Palmer","year":"2022","unstructured":"Palmer MV, Kanipe C, Boggiatto PM (2022) The bovine tuberculoid granuloma. Pathogens 11(1):61. https:\/\/doi.org\/10.3390\/pathogens11010061","journal-title":"Pathogens"},{"issue":"1","key":"11347_CR17","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1006\/cimm.2001.1819","volume":"211","author":"JE Pearl","year":"2001","unstructured":"Pearl JE, Saunders B, Ehlers S, Orme IM, Cooper AM (2001) Inflammation and lymphocyte activation during mycobacterial infection in the interferon-gamma-deficient mouse. Cell Immunol 211(1):43\u201350. https:\/\/doi.org\/10.1006\/cimm.2001.1819","journal-title":"Cell Immunol"},{"key":"11347_CR18","doi-asserted-by":"publisher","first-page":"2","DOI":"10.3389\/fmicb.2011.00002","volume":"2","author":"SJ Sasindran","year":"2011","unstructured":"Sasindran SJ, Torrelles JB (2011) Mycobacterium tuberculosis infection and inflammation: what is beneficial for the host and for the bacterium? Front Microbiol 2:2. https:\/\/doi.org\/10.3389\/fmicb.2011.00002","journal-title":"Front Microbiol"},{"key":"11347_CR19","doi-asserted-by":"publisher","first-page":"e0652016","DOI":"10.1590\/1808-1657000652016","volume":"85","author":"DAV Silva","year":"2018","unstructured":"Silva DAV, Siconelli MJL, B\u00fcrger KP, Keid LB (2018) Comparison between tests for tuberculosis diagnosis in slaughtered bovines. Arq Inst Biol 85:e0652016. https:\/\/doi.org\/10.1590\/1808-1657000652016","journal-title":"Arq Inst Biol"},{"key":"11347_CR20","doi-asserted-by":"publisher","first-page":"e0592014","DOI":"10.1590\/1808-1657000592014","volume":"83","author":"MA Souza","year":"2016","unstructured":"Souza MA, Bombonato NG, Soares PM, Ramos GB, Castro IP, Medeiros AA, Lima AMC (2016) Exames complementares no diagn\u00f3stico da tuberculose em bovinos reagentes \u00e0 tuberculiniza\u00e7\u00e3o comparada. Arq Inst Biol 83:e0592014. https:\/\/doi.org\/10.1590\/1808-1657000592014","journal-title":"Arq Inst Biol"},{"issue":"6","key":"11347_CR21","first-page":"6432","volume":"9","author":"L Sun","year":"2016","unstructured":"Sun L, Nie L, Jiang Y, Yang L, Bu H, Ye F (2016) Rapid and accurate identification of Mycobacterium tuberculosis complex by simultaneous detection of 16S rRNA and IS6110 sequence in FFPE samples. Int J Clin Exp Pathol 9(6):6432\u20136439","journal-title":"Int J Clin Exp Pathol"},{"key":"11347_CR22","doi-asserted-by":"publisher","unstructured":"Tekle Y, Gobena A (2020) Mycobacterium bovis characterization through RD4 deletion and spoligotyping techniques. Biomed J Sci Tech Res 24(4). https:\/\/doi.org\/10.26717\/BJSTR.2020.24.004074","DOI":"10.26717\/BJSTR.2020.24.004074"},{"issue":"2","key":"11347_CR23","doi-asserted-by":"publisher","first-page":"48","DOI":"10.5897\/JVMAH12.015","volume":"6","author":"D Terefe","year":"2014","unstructured":"Terefe D (2014) Gross pathological lesions of bovine tuberculosis and efficiency of meat inspection procedure to detect infected cattle in Adama municipal abattoir. J Vet Med Anim Health 6(2):48\u201353. https:\/\/doi.org\/10.5897\/JVMAH12.015","journal-title":"J Vet Med Anim Health"},{"issue":"8","key":"11347_CR24","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1590\/S0100-736X2014000800010","volume":"34","author":"B Tessele","year":"2014","unstructured":"Tessele B, Martins TB, Vielmo A, Barros CSL (2014) Les\u00f5es granulomatosas encontradas em bovinos abatidos para consumo. Pesq Vet Bras 34(8):763\u2013769. https:\/\/doi.org\/10.1590\/S0100-736X2014000800010","journal-title":"Pesq Vet Bras"},{"issue":"4","key":"11347_CR25","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1101\/gr.1.4.269","volume":"1","author":"S Wilton","year":"1992","unstructured":"Wilton S, Cousins D (1992) Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube. PCR Methods Appl 1(4):269\u2013273. https:\/\/doi.org\/10.1101\/gr.1.4.269","journal-title":"PCR Methods Appl"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11347-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11347-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11347-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T03:54:59Z","timestamp":1789790099000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11259-026-11347-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,12]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["11347"],"URL":"https:\/\/doi.org\/10.1007\/s11259-026-11347-5","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-9664864\/v1","asserted-by":"object"}]},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,12]]},"assertion":[{"value":"9 May 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 June 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"388"}}