{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:21:08Z","timestamp":1792783268265,"version":"4.5.0"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universidade De S\u00e3o Paulo","id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Wild animals often play a role in the transmission of zoonotic pathogens. Among the wild mammals of South America, ring-tailed coatis (\n                    <jats:italic>Nasua nasua<\/jats:italic>\n                    ) can present a high frequency of anti-\n                    <jats:italic>Leptospira<\/jats:italic>\n                    antibodies and carry\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. This study aimed to evaluate the frequency of serum anti-\n                    <jats:italic>Leptospira<\/jats:italic>\n                    antibodies and perform the molecular identification of\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. in ring-tailed coatis living in the Tiet\u00ea River Ecological Park, in southeastern Brazil. Blood and urine samples were obtained from ring-tailed coatis. The serum was tested using the Microscopic Agglutination Test (MAT). Urine was cultured in semi-solid Ellinghausen\u2013McCullough\u2013Johnson\u2013Harris medium. The inoculum was cultivated at 28\u00a0\u00b0C and monitored over six weeks. Total DNA from the blood and urine samples was extracted, and the 16\u00a0S rRNA gene and\n                    <jats:italic>lipL32<\/jats:italic>\n                    were searched by PCR. Of 192 animals, 36 (18.75%) had positive serum samples in the MAT. The most frequent serogroups in MAT were Grippotyphosa (11.46%; 22\/192), Autumnalis (7.81%; 15\/192) and Cynopteri (2.60%; 5\/192). A total of 116 urine samples were obtained. None of the\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. were isolated, twelve samples were positive for the 16\u00a0S rRNA gene (10.34%), and four were positive for the\n                    <jats:italic>lipL32<\/jats:italic>\n                    gene (3.4%). These results indicate a high frequency of serum anti-\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. antibodies in coatis from the Tiet\u00ea River Ecological Park and highlight the possibility of their potential role as carriers of pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. in urine.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11372-4","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T09:12:52Z","timestamp":1782897172000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Serological and molecular detection of Leptospira spp. in ring-tailed coatis (Nasua nasua) from an Ecological Park"],"prefix":"10.1007","volume":"50","author":[{"given":"Gabriel Siqueira","family":"dos Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise Batista Nogueira","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Israel Barbosa","family":"Guedes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Rodrigues","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nath\u00e1lia","family":"da Silveira Guimar\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele Oliveira","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Sim\u00f5es Sergio","family":"Petri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haroldo","family":"Furuya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Augusto","family":"Dias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Soares Ferreira","family":"Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Bryan","family":"Heinemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"11372_CR1","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/j.vetmic.2009.03.012","volume":"140","author":"B Adler","year":"2010","unstructured":"Adler B, de la Pe\u00f1a Moctezuma A (2010) Leptospira and leptospirosis. 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The approval process number of these clinical managements is 0000014501.","order":2,"name":"Ethics","label":"Statement of animal ethics","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"434"}}