{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T04:05:52Z","timestamp":1789790752549,"version":"4.0.1"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universidade Estadual Paulista J\u00falio De Mesquita Filho","id":[{"id":"https:\/\/ror.org\/00987cb86","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Bacteria of the family\n                    <jats:italic>Anaplasmataceae<\/jats:italic>\n                    are tick-borne pathogens of recognized veterinary and zoonotic relevance, widely distributed among domestic and wild vertebrate hosts. Although increasingly reported in Brazilian wildlife species, molecular data on these agents in xenarthrans remain scarce, particularly in regions undergoing environmental transformation. This study investigated the occurrence of\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    spp. and\n                    <jats:italic>Ehrlichia<\/jats:italic>\n                    spp. in free-ranging giant anteaters (\n                    <jats:italic>Myrmecophaga tridactyla<\/jats:italic>\n                    ) and southern tamanduas (\n                    <jats:italic>Tamandua tetradactyla<\/jats:italic>\n                    ) from the central-western region of S\u00e3o Paulo State, Brazil. Twenty-six blood samples (21\n                    <jats:italic>M. tridactyla<\/jats:italic>\n                    and 5\n                    <jats:italic>T. tetradactyla<\/jats:italic>\n                    ) were analyzed using conventional and nested PCR assays targeting the 23\u00a0S rRNA gene (\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    spp.) and the\n                    <jats:italic>dsb<\/jats:italic>\n                    gene (\n                    <jats:italic>Ehrlichia<\/jats:italic>\n                    spp.), followed by sequencing and phylogenetic inference. An\n                    <jats:italic>Ehrlichia<\/jats:italic>\n                    genotype phylogenetically related to\n                    <jats:italic>Ehrlichia chaffeensis<\/jats:italic>\n                    was detected in two\n                    <jats:italic>M. tridactyla<\/jats:italic>\n                    samples (7.69%), whereas an\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    genotype was detected in one\n                    <jats:italic>T. tetradactyla<\/jats:italic>\n                    sample (3.85%). The\n                    <jats:italic>Ehrlichia<\/jats:italic>\n                    sequences showed high nucleotide identity (99.61\u2013100%) with reference sequences identified as\n                    <jats:italic>E. chaffeensis<\/jats:italic>\n                    and grouped within clades containing\n                    <jats:italic>E. chaffeensis<\/jats:italic>\n                    in Maximum Likelihood analyses. The\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    sequence showed phylogenetic affinity with a clade comprising\n                    <jats:italic>Anaplasma marginale<\/jats:italic>\n                    ,\n                    <jats:italic>Anaplasma centrale<\/jats:italic>\n                    , and related genotypes. Because only partial gene fragments were analyzed, species-level assignment was not attempted. These findings provide molecular evidence of the occurrence of\n                    <jats:italic>Anaplasmataceae<\/jats:italic>\n                    -related genotypes in free-ranging xenarthrans inhabiting anthropogenically altered landscapes in southeastern Brazil. Further investigations including expanded sampling, vector identification, and multilocus or genomic approaches are necessary to better characterize these agents and clarify their ecological significance.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11375-1","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T04:55:58Z","timestamp":1782795358000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular detection and phylogenetic characterization of Ehrlichia- and Anaplasma-related genotypes in free-ranging anteaters from southeastern Brazil"],"prefix":"10.1007","volume":"50","author":[{"given":"Jo\u00e3o Ot\u00e1vio","family":"Mochiuti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus Oliveira","family":"Machado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edgia Souza Lima Silveira","family":"da Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"11375_CR1","doi-asserted-by":"publisher","first-page":"962","DOI":"10.3390\/microorganisms12050962","volume":"12","author":"AS Alab\u00ed C\u00f3rdova","year":"2024","unstructured":"Alab\u00ed C\u00f3rdova AS, Fecchio A, Calchi AC, Dias CM, Mongruel ACB, Neves LF, Lee DAB, Machado RZ, Andr\u00e9 MR (2024) Novel tick-borne Anaplasmataceae genotypes in tropical birds from the Brazilian Pantanal Wetland. 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Sampling and handling protocols were approved by the Animal Use Ethics Committee of S\u00e3o Paulo State University (CEUA\u2013UNESP; protocol no. 000.051\/2024) and authorized by the Brazilian Biodiversity Authorization and Information System (SISBIO; permit no. 86964-1).","order":1,"name":"Ethics","label":"Ethics approval and consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"433"}}