{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T08:34:12Z","timestamp":1793003652540,"version":"4.5.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universidade Estadual Paulista J\u00falio De Mesquita Filho","id":[{"id":"https:\/\/ror.org\/00987cb86","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Eimeria lata<\/jats:italic>\n                    is a recently characterized species of domestic chicken whose sensitivity to anticoccidial drugs remains largely unknown. This study aimed to determine the anticoccidial sensitivity profile of the Brazilian\n                    <jats:italic>E. lata<\/jats:italic>\n                    BR-AMC isolate, obtained from alternative production systems, through an anticoccidial sensitivity test (AST). One hundred ninety-two broiler chicks were allocated into six treatment groups: unmedicated, non-challenged; unmedicated, challenged; and challenged groups medicated with nicarbazin (100 ppm), salinomycin (72 ppm), lasalocid (75 ppm), or semduramicin (22.5 ppm). Birds were orally challenged with 20,000 sporulated oocysts. Sensitivity was assessed using three complementary parameters: oocyst output reduction, the percentage of optimum anticoccidial activity (POAA), and body weight gain (BWG). All tested drugs reduced oocyst output by more than 80%, indicating sensitivity to all of them by this parameter. Nevertheless, based on POAA,\n                    <jats:italic>E. lata<\/jats:italic>\n                    was sensitive to salinomycin (96%) and lasalocid (130%), partially sensitive to semduramicin (72%), and resistant to nicarbazin (40%). BWG results demonstrated sensitivity to lasalocid, salinomycin and semduramicin, while results for nicarbazin were inconclusive. When taken together and based on the interpretation criteria adopted in this study, these divergent results indicate reduced sensitivity to nicarbazin and sensitivity to lasalocid, salinomycin, and semduramicin, underlining the importance of multi-parameter approaches when conducting ASTs. Furthermore, although based on a single isolate, our results also raise the possibility that alternative rearing systems may harbor drug-resistant\n                    <jats:italic>Eimeria<\/jats:italic>\n                    strains, an epidemiological concern that warrants further investigation.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11480-1","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T04:25:40Z","timestamp":1787631940000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First anticoccidial sensitivity test applied to Eimeria lata: evidence of reduced sensitivity to nicarbazin in a Brazilian field isolate"],"prefix":"10.1007","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7856-6047","authenticated-orcid":false,"given":"Giovanna Fernandes","family":"Esteves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7901-9019","authenticated-orcid":false,"given":"Bruno Ferraz","family":"Itoyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6907-409X","authenticated-orcid":false,"given":"Brayan","family":"Kurahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Pav\u00e3o Saraiva","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Mapeli","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5574-0183","authenticated-orcid":false,"given":"Manoel","family":"Garcia Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Vasconcelos","family":"Meireles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"11480_CR1","doi-asserted-by":"publisher","first-page":"1485","DOI":"10.3382\/ps.0691485","volume":"69","author":"KW Bafundo","year":"1990","unstructured":"Bafundo KW, Jeffers TK (1990) Selection for resistance to monensin, nicarbazin, and the monensin plus nicarbazin combination. Poult Sci 69:1485\u20131490. https:\/\/doi.org\/10.3382\/ps.0691485","journal-title":"Poult Sci"},{"key":"11480_CR2","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1186\/s13567-020-00837-2","volume":"51","author":"DP Blake","year":"2020","unstructured":"Blake DP, Knox J, Dehaeck B, Huntington B, Rathinam T, Ravipati V, Ayoade S, Gilbert W, Adebambo O, Jatau ID, Raman M, Parker D, Rushton J, Tomley FM (2020) Re-calculating the cost of coccidiosis in chickens. Vet Res 51:115. https:\/\/doi.org\/10.1186\/s13567-020-00837-2","journal-title":"Vet Res"},{"key":"11480_CR3","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1016\/j.ijpara.2020.12.004","volume":"51","author":"DP Blake","year":"2021","unstructured":"Blake DP, Vrba V, Xia D, Jatau ID, Spiro S, Nolan MJ, Underwood G, Tomley FM (2021) Genetic and biological characterisation of three cryptic Eimeria operational taxonomic units that infect chickens (Gallus gallus domesticus). Int J Parasitol 51:621\u2013634. https:\/\/doi.org\/10.1016\/j.ijpara.2020.12.004","journal-title":"Int J Parasitol"},{"key":"11480_CR4","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1016\/j.vetpar.2007.12.028","volume":"152","author":"C Cantacessi","year":"2008","unstructured":"Cantacessi C, Riddell S, Morris GM, Doran T, Woods WG, Otranto D, Gasser RB (2008) Genetic characterization of three unique operational taxonomic units of Eimeria from chickens in Australia based on nuclear spacer ribosomal DNA. Vet Parasitol 152:226\u2013234. https:\/\/doi.org\/10.1016\/j.vetpar.2007.12.028","journal-title":"Vet Parasitol"},{"key":"11480_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1637\/21-00110","volume":"66","author":"HM Cervantes","year":"2022","unstructured":"Cervantes HM, McDougald LR (2022) The use of anticoccidial sensitivity tests (ASTs) by the poultry industry. Avian Dis 66:1\u20135. https:\/\/doi.org\/10.1637\/21-00110","journal-title":"Avian Dis"},{"key":"11480_CR6","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1080\/03079458008418387","volume":"9","author":"HD Chapman","year":"1980","unstructured":"Chapman HD (1980) Studies on the sensitivity of field isolates of Eimeria maxima to combinations of anticoccidial drugs. Avian Pathol 9:67\u201376. https:\/\/doi.org\/10.1080\/03079458008418387","journal-title":"Avian Pathol"},{"key":"11480_CR7","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/0304-4017(84)90106-7","volume":"15","author":"HD Chapman","year":"1984","unstructured":"Chapman HD (1984) Drug resistance in avian coccidia (a review). Vet Parasitol 15:11\u201327. https:\/\/doi.org\/10.1016\/0304-4017(84)90106-7","journal-title":"Vet Parasitol"},{"key":"11480_CR8","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1080\/03079459708419208","volume":"26","author":"HD Chapman","year":"1997","unstructured":"Chapman HD (1997) Biochemical, genetic and applied aspects of drug resistance in Eimeria parasites of the fowl. Avian Pathol 26:221\u2013244. https:\/\/doi.org\/10.1080\/03079459708419208","journal-title":"Avian Pathol"},{"key":"11480_CR9","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1016\/S0020-7519(98)00024-1","volume":"28","author":"HD Chapman","year":"1998","unstructured":"Chapman HD (1998) Evaluation of the efficacy of anticoccidial drugs against Eimeria species in the fowl. Int J Parasitol 28:1141\u20131144. https:\/\/doi.org\/10.1016\/S0020-7519(98)00024-1","journal-title":"Int J Parasitol"},{"key":"11480_CR10","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1080\/03079459994317","volume":"28","author":"HD Chapman","year":"1999","unstructured":"Chapman HD (1999) Anticoccidial drugs and their effects upon the development of immunity to Eimeria infections in poultry. Avian Pathol 28:521\u2013535. https:\/\/doi.org\/10.1080\/03079459994317","journal-title":"Avian Pathol"},{"key":"11480_CR11","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.ijpddr.2022.01.001","volume":"18","author":"HD Chapman","year":"2022","unstructured":"Chapman HD, Rathinam T (2022) Focused review: the role of drug combinations for the control of coccidiosis in commercially reared chickens. Int J Parasitol Drugs Drug Resist 18:32\u201342. https:\/\/doi.org\/10.1016\/j.ijpddr.2022.01.001","journal-title":"Int J Parasitol Drugs Drug Resist"},{"key":"11480_CR12","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1016\/j.ijpara.2016.05.006","volume":"46","author":"EL Clark","year":"2016","unstructured":"Clark EL, Macdonald SE, Thenmozhi V, Kundu K, Garg R, Kumar S, Ayoade S, Fornace KM, Jatau ID, Moftah A, Nolan MJ, Sudhakar NR, Adebambo AO, Lawal IA, Zapata RA, Awuni JA, Chapman HD, Karimuribo E, Mugasa CM, Namangala B, Rushton J, Suo X, Thangaraj K, Rao ASRS, Tewari AK, Banerjee PS, Raj GD, Raman M, Tomley FM, Blake DP (2016) Cryptic Eimeria genotypes are common across the southern but not northern hemisphere. Int J Parasitol 46:537\u2013544. https:\/\/doi.org\/10.1016\/j.ijpara.2016.05.006","journal-title":"Int J Parasitol"},{"key":"11480_CR13","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1126\/science.122.3162.244.b","volume":"122","author":"AC Cuckler","year":"1955","unstructured":"Cuckler AC, Malanga CM, Basso AJ, O\u2019Neill RC (1955) Antiparasitic activity of substituted carbanilide complexes. Science 122:244\u2013245. https:\/\/doi.org\/10.1126\/science.122.3162.244.b","journal-title":"Science"},{"key":"11480_CR14","doi-asserted-by":"publisher","first-page":"e001126","DOI":"10.1590\/S1984-29612026032","volume":"35","author":"GZ Dumalakas","year":"2026","unstructured":"Dumalakas GZ, Beretta BMS, Kataoka P, Itoyama BF, Kurahara B, Meireles MV (2026) NGS-based characterization of Eimeria spp. prevalence and diversity in broiler farms in northwestern S\u00e3o Paulo State, Brazil. Rev Bras Parasitol Vet 35:e001126. https:\/\/doi.org\/10.1590\/S1984-29612026032","journal-title":"Rev Bras Parasitol Vet"},{"key":"11480_CR15","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1186\/s12917-022-03369-3","volume":"18","author":"RA Flores","year":"2022","unstructured":"Flores RA, Nguyen BT, Cammayo EO, V\u00f5 TC, Naw H, Kim S, Kim WH, Na BK, Min W (2022) Epidemiological investigation and drug resistance of Eimeria species in Korean chicken farms. BMC Vet Res 18:277. https:\/\/doi.org\/10.1186\/s12917-022-03369-3","journal-title":"BMC Vet Res"},{"key":"11480_CR16","doi-asserted-by":"publisher","first-page":"e84254","DOI":"10.1371\/journal.pone.0084254","volume":"8","author":"KM Fornace","year":"2013","unstructured":"Fornace KM, Clark EL, Macdonald SE, Namangala B, Karimuribo E, Awuni JA, Thieme O, Blake DP, Rushton J (2013) Occurrence of Eimeria species parasites on small-scale commercial chicken farms in Africa and indication of economic profitability. PLoS ONE 8:e84254. https:\/\/doi.org\/10.1371\/journal.pone.0084254","journal-title":"PLoS ONE"},{"key":"11480_CR17","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.vetpar.2015.09.030","volume":"214","author":"RM Godwin","year":"2015","unstructured":"Godwin RM, Morgan JA (2015) A molecular survey of Eimeria in chickens across Australia. Vet Parasitol 214:16\u201321. https:\/\/doi.org\/10.1016\/j.vetpar.2015.09.030","journal-title":"Vet Parasitol"},{"key":"11480_CR18","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1637\/aviandiseases-d-19-00104","volume":"63","author":"R Hauck","year":"2019","unstructured":"Hauck R, Carrisosa M, McCrea BA, Dormitorio T, Macklin KS (2019) Evaluation of next-generation amplicon sequencing to identify Eimeria spp. of chickens. Avian Dis 63:577\u2013583. https:\/\/doi.org\/10.1637\/aviandiseases-d-19-00104","journal-title":"Avian Dis"},{"key":"11480_CR19","doi-asserted-by":"publisher","first-page":"176","DOI":"10.3389\/fvets.2018.00176","volume":"5","author":"AT Hinsu","year":"2018","unstructured":"Hinsu AT, Thakkar JR, Koringa PG, Vrba V, Jakhesara SJ, Psifidi A, Guitian J, Tomley FM, Rank DN, Raman M, Joshi CG, Blake DP (2018) Illumina next generation sequencing for the analysis of Eimeria populations in commercial broilers and indigenous chickens. Front Vet Sci 5:176. https:\/\/doi.org\/10.3389\/fvets.2018.00176","journal-title":"Front Vet Sci"},{"key":"11480_CR20","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.vetpar.2004.03.006","volume":"121","author":"PA Holdsworth","year":"2004","unstructured":"Holdsworth PA, Conway DP, McKenzie ME, Dayton AD, Chapman HD, Mathis GF, Skinner JT, Mundt HC, Williams RB (2004) World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines for evaluating the efficacy of anticoccidial drugs in chickens and turkeys. Vet Parasitol 121:189\u2013212. https:\/\/doi.org\/10.1016\/j.vetpar.2004.03.006","journal-title":"Vet Parasitol"},{"key":"11480_CR21","doi-asserted-by":"publisher","first-page":"101265","DOI":"10.1016\/j.vprsr.2025.101265","volume":"61","author":"BF Itoyama","year":"2025","unstructured":"Itoyama BF, Berto BP, Dumalakas GZ, Kurahara B, Furlaneti EA, Meireles MV (2025) Morphological and molecular characterization of a pure isolate of Eimeria lata, identified for the first time in domestic chickens in Brazil. Vet Parasitol Reg Stud Rep 61:101265. https:\/\/doi.org\/10.1016\/j.vprsr.2025.101265","journal-title":"Vet Parasitol Reg Stud Rep"},{"key":"11480_CR22","doi-asserted-by":"publisher","first-page":"110068","DOI":"10.1016\/j.vetpar.2023.110068","volume":"324","author":"JM Jaramillo-Ortiz","year":"2023","unstructured":"Jaramillo-Ortiz JM, Burrel C, Adeyemi O, Werling D, Blake DP (2023) First detection and characterisation of Eimeria zaria in European chickens. Vet Parasitol 324:110068. https:\/\/doi.org\/10.1016\/j.vetpar.2023.110068","journal-title":"Vet Parasitol"},{"key":"11480_CR23","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1186\/s12917-016-0713-9","volume":"12","author":"ID Jatau","year":"2016","unstructured":"Jatau ID, Lawal IA, Kwaga JKP, Tomley FM, Blake DP, Nok AJ (2016) Three operational taxonomic units of Eimeria are common in Nigerian chickens and may undermine effective molecular diagnosis of coccidiosis. BMC Vet Res 12:86. https:\/\/doi.org\/10.1186\/s12917-016-0713-9","journal-title":"BMC Vet Res"},{"key":"11480_CR24","doi-asserted-by":"publisher","first-page":"101233","DOI":"10.1016\/j.psj.2021.101233","volume":"100","author":"AL Kraieski","year":"2021","unstructured":"Kraieski AL, Salles GBC, Muniz EC, Nascimento DVJ, Neto AL, Santos IL, Madeira AMBN (2021) Sensitivity of field isolates of Eimeria acervulina and E. maxima from three regions in Brazil to eight anticoccidial drugs. Poult Sci 100:101233. https:\/\/doi.org\/10.1016\/j.psj.2021.101233","journal-title":"Poult Sci"},{"key":"11480_CR25","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.vetpar.2003.12.009","volume":"119","author":"GQ Li","year":"2004","unstructured":"Li GQ, Kanu S, Xiang FY, Xiao SM, Zhang L, Chen HW, Ye HJ (2004) Isolation and selection of ionophore-tolerant Eimeria precocious lines: E. tenella, E. maxima and E. acervulina. Vet Parasitol 119:261\u2013276. https:\/\/doi.org\/10.1016\/j.vetpar.2003.12.009","journal-title":"Vet Parasitol"},{"key":"11480_CR27","first-page":"201","volume":"6","author":"PL Long","year":"1976","unstructured":"Long PL, Millard BJ, Joyner LP, Norton CC (1976) A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis. Folia Vet Lat 6:201\u2013217","journal-title":"Folia Vet Lat"},{"key":"11480_CR26","doi-asserted-by":"publisher","first-page":"384","DOI":"10.3389\/fvets.2020.00384","volume":"7","author":"S L\u00f3pez-Osorio","year":"2020","unstructured":"L\u00f3pez-Osorio S, Chaparro-Guti\u00e9rrez JJ, G\u00f3mez-Osorio LM (2020) Overview of poultry Eimeria life cycle and host-parasite interactions. Front Vet Sci 7:384. https:\/\/doi.org\/10.3389\/fvets.2020.00384","journal-title":"Front Vet Sci"},{"key":"11480_CR29","doi-asserted-by":"publisher","first-page":"104663","DOI":"10.1016\/j.psj.2024.104663","volume":"104","author":"GF Mathis","year":"2025","unstructured":"Mathis GF, Lumpkins B, Cervantes HM, Fitz-Coy SH, Jenkins MC, Jones MK, Price KR, Dalloul RA (2025) Coccidiosis in poultry: disease mechanisms, control strategies, and future directions. Poult Sci 104:104663. https:\/\/doi.org\/10.1016\/j.psj.2024.104663","journal-title":"Poult Sci"},{"key":"11480_CR28","doi-asserted-by":"publisher","first-page":"600","DOI":"10.2307\/1589990","volume":"25","author":"LR McDougald","year":"1981","unstructured":"McDougald LR (1981) Anticoccidial drug resistance in the Southeastern United States: polyether, ionophorous drugs. Avian Dis 25:600\u2013609. https:\/\/doi.org\/10.2307\/1589990","journal-title":"Avian Dis"},{"key":"11480_CR30","doi-asserted-by":"publisher","first-page":"101239","DOI":"10.1016\/j.psj.2021.101239","volume":"100","author":"C Mesa","year":"2021","unstructured":"Mesa C, G\u00f3mez-Osorio LM, L\u00f3pez-Osorio S, Williams SM, Chaparro-Guti\u00e9rrez JJ (2021) Survey of coccidia on commercial broiler farms in Colombia: frequency of Eimeria species, anticoccidial sensitivity, and histopathology. Poult Sci 100:101239. https:\/\/doi.org\/10.1016\/j.psj.2021.101239","journal-title":"Poult Sci"},{"key":"11480_CR31","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1007\/s00436-007-0516-9","volume":"101","author":"GM Morris","year":"2007","unstructured":"Morris GM, Woods WG, Richards DG, Gasser RB (2007) Investigating a persistent coccidiosis problem on a commercial broiler\u2013breeder farm utilising PCR-coupled capillary electrophoresis. Parasitol Res 101:583\u2013589. https:\/\/doi.org\/10.1007\/s00436-007-0516-9","journal-title":"Parasitol Res"},{"key":"11480_CR32","doi-asserted-by":"publisher","first-page":"2009","DOI":"10.1007\/s00436-019-06343-5","volume":"118","author":"S Noack","year":"2019","unstructured":"Noack S, Chapman HD, Selzer PM (2019) Anticoccidial drugs of the livestock industry. Parasitol Res 118:2009\u20132026. https:\/\/doi.org\/10.1007\/s00436-019-06343-5","journal-title":"Parasitol Res"},{"key":"11480_CR33","doi-asserted-by":"publisher","first-page":"110759","DOI":"10.1016\/j.vetpar.2026.110759","volume":"344","author":"M Raffaelli","year":"2026","unstructured":"Raffaelli M, Jaramillo-Ortiz JM, Vasilogianni M, Hay MC, Ngugi D, Burrell C, Marugan-Hernandez V, Perrucci S, Xia D, Blake DP (2026) Ionophore susceptibility of Eimeria zaria: first characterisation in a cryptic Eimeria species of chickens. Vet Parasitol 344:110759. https:\/\/doi.org\/10.1016\/j.vetpar.2026.110759","journal-title":"Vet Parasitol"},{"key":"11480_CR34","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1637\/8679-030509-Reg.1","volume":"53","author":"T Rathinam","year":"2009","unstructured":"Rathinam T, Chapman HD (2009) Sensitivity of isolates of Eimeria from turkey flocks to the anticoccidial drugs amprolium, clopidol, diclazuril, and monensin. Avian Dis 53:405\u2013408. https:\/\/doi.org\/10.1637\/8679-030509-Reg.1","journal-title":"Avian Dis"},{"key":"11480_CR35","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1186\/s13071-017-2067-y","volume":"10","author":"L Tan","year":"2017","unstructured":"Tan L, Liu Y, Yang X, Ke Q, Lei W, Mughal MN, Fang R, Zhou Y, Shen B, Zhao J (2017) Genetic diversity and drug sensitivity studies on Eimeria tenella field isolates from Hubei Province of China. Parasites Vectors 10:137. https:\/\/doi.org\/10.1186\/s13071-017-2067-y","journal-title":"Parasites Vectors"},{"key":"11480_CR36","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1637\/aviandiseases-d-20-00120","volume":"65","author":"MTB Terra","year":"2021","unstructured":"Terra MTB, Pacheco WJ, Harrison M, McCrea BA, Hauck RA (2021) Survey of coccidia and nematodes in pastured poultry in the state of Georgia. Avian Dis 65:250\u2013256. https:\/\/doi.org\/10.1637\/aviandiseases-d-20-00120","journal-title":"Avian Dis"},{"key":"11480_CR37","unstructured":"The Jamovi Project (2025) Jamovi (Version 2.7) [Computer Software]. Retrieved from https:\/\/www.jamovi.org"},{"key":"11480_CR38","doi-asserted-by":"publisher","first-page":"102819","DOI":"10.1016\/j.psj.2023.102819","volume":"102","author":"C Trujillo-Peralta","year":"2023","unstructured":"Trujillo-Peralta C, Ashcraft A, Se\u00f1as-Cuesta R, Coles M, Hernandez-Velasco X, Selby C, Forga A, Tellez-Isaias G, Vuong C, Bielke L, Barta J, Hargis B, Graham D (2023) Research note: isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States. Poult Sci 102:102819. https:\/\/doi.org\/10.1016\/j.psj.2023.102819","journal-title":"Poult Sci"},{"key":"11480_CR39","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/j.vetpar.2010.09.006","volume":"174","author":"V Vrba","year":"2010","unstructured":"Vrba V, Blake DP, Poplstein M (2010) Quantitative real-time PCR assays for detection and quantification of all seven Eimeria species that infect the chicken. Vet Parasitol 174:183\u2013190. https:\/\/doi.org\/10.1016\/j.vetpar.2010.09.006","journal-title":"Vet Parasitol"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11480-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11480-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11480-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T08:02:23Z","timestamp":1793001743000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11480-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,25]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["11480"],"URL":"https:\/\/doi.org\/10.1007\/s11259-026-11480-1","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,25]]},"assertion":[{"value":"13 June 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 August 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 August 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This study was conducted in line with the principles of the National Council for the Control of Animal Experimentation (CONCEA) and approved by the Animal Use Ethics Committee (CEUA) of S\u00e3o Paulo State University (Unesp), School of Veterinary Medicine, Ara\u00e7atuba (protocol FOA 742\/2025).","order":1,"name":"Ethics","label":"Ethics approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"528"}}