{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T05:37:23Z","timestamp":1792820243230,"version":"4.5.0"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universidade Estadual De Londrina","id":[{"id":"https:\/\/ror.org\/01585b035","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Dairy farming is one of the most widespread agricultural activities in Brazil. Neonatal diarrhea remains a major challenge, contributing significantly to economic and production losses in Brazilian dairy cattle herds. Picobirnavirus (PBV) has been suggested as a potential viral agent associated with calf diarrhea. This study aimed to detect PBV genogroup I (GI) RNA in fecal samples from calves in a closed high-producing dairy herd in S\u00e3o Paulo, southeastern Brazil. A total of 122 fecal samples were collected from 30-day-old calves, including diarrheic (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200916) and non-diarrheic (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009106) animals. Nucleic acids were extracted and analyzed using reverse transcription-polymerase chain reaction (RT-PCR). PBV GI was detected in 54.1% (66\/122) of the samples, with the majority in non-diarrheic calves (87.9%; 58\/66). The detection rate was 50.0% (8\/16) in diarrheic calves and 54.7% (58\/106) in non-diarrheic calves, with no significant association between PBV GI presence and fecal consistency (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.72). Two RT-PCR amplicons from fecal samples collected on different days were sequenced to confirm detection of PBV GI; however, only one nucleotide (nt) sequence presented sufficient quality for analysis. Phylogenetic analysis revealed that this sequence clustered within PBV GI and shared the highest nt identity with a raw sewage sequence (93.4%), two human sequences (87.8\u201389.8%), and a monkey sequence (88.8%). These results confirm the circulation of PBV GI in a dairy herd and suggest that its presence is not associated with calf diarrhea. Further studies are needed to clarify the epidemiological and biological significance of PBV GI in cattle, as well as its impact on animal health and production.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11510-y","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:19:48Z","timestamp":1789172388000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High prevalence of picobirnavirus genogroup I in calves without association with diarrhea in a dairy cattle herd, southeast Brazil"],"prefix":"10.1007","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7055-6549","authenticated-orcid":false,"given":"Geovana Depieri","family":"Yoshitani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9167-5518","authenticated-orcid":false,"given":"Denise Correia","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9764-1460","authenticated-orcid":false,"given":"Felipe Augusto","family":"de Brito Vargas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1850-0498","authenticated-orcid":false,"given":"Marcos Vinicius","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4635-5512","authenticated-orcid":false,"given":"Juliana Torres Tomazi","family":"Fritzen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8607-956X","authenticated-orcid":false,"given":"Alice Fernandes","family":"Alfieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7024-4487","authenticated-orcid":false,"given":"Amauri Alcindo","family":"Alfieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5032-712X","authenticated-orcid":false,"given":"Elis","family":"Lorenzetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,12]]},"reference":[{"issue":"7","key":"11510_CR1","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1007\/s11250-006-4349-9","volume":"38","author":"AA Alfieri","year":"2006","unstructured":"Alfieri AA, Parazzi ME, Takiuchi E, M\u00e9dici KC, Alfieri AF (2006) Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998\u20132002. Trop Anim Health Prod 38(7):521\u2013526. https:\/\/doi.org\/10.1007\/s11250-006-4349-9","journal-title":"Trop Anim Health Prod"},{"issue":"2","key":"11510_CR2","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1007\/s11250-022-03128-4","volume":"54","author":"MO Atasoy","year":"2022","unstructured":"Atasoy MO, Isidan H, Turan T (2022) Genetic diversity, frequency and concurrent infections of picobirnaviruses in diarrhoeic calves in Turkey. Trop Anim Health Prod 54(2):127. https:\/\/doi.org\/10.1007\/s11250-022-03128-4","journal-title":"Trop Anim Health Prod"},{"issue":"5","key":"11510_CR3","first-page":"509","volume":"30","author":"F Bendali","year":"1999","unstructured":"Bendali F, Sanaa M, Bichet H, Schelcher F (1999) Risk factors associated with diarrhoea in newborn calves. Vet Res 30(5):509\u2013522","journal-title":"Vet Res"},{"issue":"2","key":"11510_CR4","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/S1090-0233(03)00031-5","volume":"166","author":"MG Buzinaro","year":"2003","unstructured":"Buzinaro MG, Freitas PPS, Kisiellius JJ, Ueda M, Jerez JA (2003) Identification of a bisegmented double-stranded RNA virus (picobirnavirus) in calf faeces. Vet J 166(2):185\u2013187. https:\/\/doi.org\/10.1016\/S1090-0233(03)00031-5","journal-title":"Vet J"},{"issue":"1","key":"11510_CR5","doi-asserted-by":"publisher","first-page":"74","DOI":"10.3390\/zoonoticdis4010008","volume":"4","author":"EHN Chagas","year":"2024","unstructured":"Chagas EHN, Da Silva JR, Barros BCV, Duarte J\u00fanior JWB, Santos FS, Sousa J\u00fanior EC, Bezerra DAM, Santos MI, Pinheiro HHC, Malik YS, Mascarenhas JDP (2024) Occurrence of picobirnavirus in domestic and wild animals from three cities of Brazilian Amazon. Zoonotic Dis 4(1):74\u201385. https:\/\/doi.org\/10.3390\/zoonoticdis4010008","journal-title":"Zoonotic Dis"},{"issue":"3\u20134","key":"11510_CR6","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/j.vetmic.2013.07.001","volume":"166","author":"Y-I Cho","year":"2013","unstructured":"Cho Y-I, Han J-I, Wang C, Cooper V, Schwartz K, Engelken T, Yoon K-J (2013) Case\u2013control study of microbiological etiology associated with calf diarrhea. Vet Microbiol 166(3\u20134):375\u2013385. https:\/\/doi.org\/10.1016\/j.vetmic.2013.07.001","journal-title":"Vet Microbiol"},{"issue":"2","key":"11510_CR7","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1099\/jgv.0.001186","volume":"100","author":"B Delmas","year":"2019","unstructured":"Delmas B, Attoui H, Ghosh S, Malik YS, Mundt E, Vakharia VN, ICTV Report Consortium (2019) ICTV virus taxonomy profile: Picobirnaviridae. J Gen Virol 100(2):133\u2013134. https:\/\/doi.org\/10.1099\/jgv.0.001186","journal-title":"J Gen Virol"},{"key":"11510_CR8","unstructured":"ICTV (2025) International Committee on Taxonomy of Viruses. Master Species List (MSL41) Update March 20th 2026. Accessed 31 May 2026 https:\/\/ictv.global\/taxonomy"},{"key":"11510_CR9","doi-asserted-by":"publisher","first-page":"105456","DOI":"10.1016\/j.meegid.2023.105456","volume":"112","author":"MA Knox","year":"2023","unstructured":"Knox MA, Wierenga J, Biggs PJ, Gedye K, Almeida V, Hall R, Kalema-Zikusoka G, Rubanga S, Ngabirano A, Valdivia-Granda W, Hayman DTS (2023) Abundant dsRNA picobirnaviruses show little geographic or host association in terrestrial systems. Infect Genet Evol 112:105456. https:\/\/doi.org\/10.1016\/j.meegid.2023.105456","journal-title":"Infect Genet Evol"},{"issue":"8","key":"11510_CR10","doi-asserted-by":"publisher","first-page":"1475","DOI":"10.1007\/s11250-011-9834-0","volume":"43","author":"YS Malik","year":"2011","unstructured":"Malik YS, Chandrashekar KM, Sharma K, Haq AA, Vaid N, Chakravarti S, Batra M, Singh R, Pandey AB (2011) Picobirnavirus detection in bovine and buffalo calves from foothills of Himalaya and Central India. Trop Anim Health Prod 43(8):1475\u20131478. https:\/\/doi.org\/10.1007\/s11250-011-9834-0","journal-title":"Trop Anim Health Prod"},{"issue":"1","key":"11510_CR11","doi-asserted-by":"publisher","first-page":"780752","DOI":"10.1155\/2014\/780752","volume":"2014","author":"YS Malik","year":"2014","unstructured":"Malik YS, Kumar N, Sharma K, Dhama K, Shabbir MZ, Ganesh B, Kobayashi N, Banyai K (2014) Epidemiology, phylogeny, and evolution of emerging enteric Picobirnaviruses of animal origin and their relationship to human strains. BioMed Res Int 2014(1):780752. https:\/\/doi.org\/10.1155\/2014\/780752","journal-title":"BioMed Res Int"},{"key":"11510_CR12","doi-asserted-by":"publisher","first-page":"99","DOI":"10.2174\/1874357901812010099","volume":"12","author":"YS Malik","year":"2018","unstructured":"Malik YS, Sircar S, Saurabh S, Kattoor JJ, Singh R, Ganesh B, Ghosh S, Dhama K, Singh RK (2018) Epidemiologic status of picobirnavirus in India, a less explored viral disease. Open Virol J 12:99\u2013109. https:\/\/doi.org\/10.2174\/1874357901812010099","journal-title":"Open Virol J"},{"issue":"5","key":"11510_CR13","doi-asserted-by":"publisher","first-page":"724","DOI":"10.1007\/s11262-018-1586-8","volume":"54","author":"JO Navarro","year":"2018","unstructured":"Navarro JO, Candido M, de Almeida-Queiroz SR, Buzinaro MG, Livonesi MC, Fernandes AM, de Sousa RLM (2018) Genetic diversity of bovine Picobirnavirus, Brazil. Virus Genes 54(5):724\u2013728. https:\/\/doi.org\/10.1007\/s11262-018-1586-8","journal-title":"Virus Genes"},{"issue":"3","key":"11510_CR14","doi-asserted-by":"publisher","first-page":"319","DOI":"10.30466\/vrf.2020.110016.2620","volume":"12","author":"A Nazaktabar","year":"2021","unstructured":"Nazaktabar A (2021) Molecular epidemiology and phylogenetic analysis of bovine picobirnaviruses causing calf diarrhea, in Iran. Vet Res Forum 12(3):319\u2013324. https:\/\/doi.org\/10.30466\/vrf.2020.110016.2620","journal-title":"Vet Res Forum"},{"issue":"10","key":"11510_CR15","doi-asserted-by":"publisher","first-page":"5340","DOI":"10.1128\/jvi.00064-15","volume":"89","author":"TFF Ng","year":"2015","unstructured":"Ng TFF, Kondov NO, Deng X, Van Eenennaam A, Neibergs HL, Delwart E (2015) A metagenomics and case-control study to identify viruses associated with bovine respiratory disease. J Virol 89(10):5340\u20135349. https:\/\/doi.org\/10.1128\/jvi.00064-15","journal-title":"J Virol"},{"issue":"2","key":"11510_CR16","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1006\/viro.2000.0594","volume":"277","author":"BI Rosen","year":"2000","unstructured":"Rosen BI, Fang Z-Y, Glass RI, Monroe SS (2000) Cloning of human picobirnavirus genomic segments and development of an RT-PCR detection assay. Virology 277(2):316\u2013329. https:\/\/doi.org\/10.1006\/viro.2000.0594","journal-title":"Virology"},{"key":"11510_CR17","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1038\/nature20167","volume":"540","author":"M Shi","year":"2016","unstructured":"Shi M, Lin X-D, Tian J-H, Chen L-J, Chen X, Li C-X, Qin X-C, Li J, Cao J-P, Eden J-S, Buchmann J, Wang W, Xu J, Holmes EC, Zhang Y-Z (2016) Redefining the invertebrate RNA virosphere. Nature 540:539\u2013543. https:\/\/doi.org\/10.1038\/nature20167","journal-title":"Nature"},{"key":"11510_CR18","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.virusres.2015.09.022","volume":"211","author":"E Takiuchi","year":"2016","unstructured":"Takiuchi E, Macedo R, Kunz AF, Gallego JC, de Mello JL, Otonel RAA, Alfieri AA (2016) Electrophoretic RNA genomic profiles of Brazilian Picobirnavirus (PBV) strains and molecular characterization of a PBV isolated from diarrheic calf. Virus Res 211:58\u201363. https:\/\/doi.org\/10.1016\/j.virusres.2015.09.022","journal-title":"Virus Res"},{"issue":"2","key":"11510_CR19","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.prevetmed.2013.10.019","volume":"113","author":"MC Windeyer","year":"2014","unstructured":"Windeyer MC, Leslie KE, Godden SM, Hodgins DC, Lissemore KD, LeBlanc SJ (2014) Factors associated with morbidity, mortality, and growth of dairy heifer calves up to 3 months of age. Prev Vet Med 113(2):231\u2013240. https:\/\/doi.org\/10.1016\/j.prevetmed.2013.10.019","journal-title":"Prev Vet Med"},{"issue":"6","key":"11510_CR20","doi-asserted-by":"publisher","first-page":"574","DOI":"10.3390\/v11060574","volume":"11","author":"PCY Woo","year":"2019","unstructured":"Woo PCY, Teng JLL, Bai R, Tang Y, Wong AYP, Li KSM, Lam CSF, Fan RYY, Lau SKP, Yuen K-Y (2019) Novel picobirnaviruses in respiratory and alimentary tracts of cattle and monkeys with large intra-and inter-host diversity. Viruses 11(6):574. https:\/\/doi.org\/10.3390\/v11060574","journal-title":"Viruses"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11510-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11510-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11510-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T04:44:38Z","timestamp":1792817078000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11510-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,12]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["11510"],"URL":"https:\/\/doi.org\/10.1007\/s11259-026-11510-y","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,12]]},"assertion":[{"value":"16 June 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 September 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This study was approved by the Ethics Committee on Animal Use of the Universidade Estadual de Londrina under process n. 072\/2013, n. 6371.2013.43. All applicable international, national, and institutional guidelines for the care and use of animals were followed.","order":1,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"564"}}