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The increasing use of controlled breeding programs highlights the need for effective viral inactivation strategies. This study evaluated the antiviral activity of the antimicrobial peptide indolicidin in bovine semen experimentally inoculated with BoAHV1. Indolicidin was synthesized by solid-phase peptide synthesis. Antiviral activity and minimum inhibitory concentration (MIC) were determined in MDBK cell cultures. The peptide was then tested in inoculated semen, and semen quality was assessed using computer-assisted sperm analysis (CASA). Indolicidin inhibited BoAHV1 in MDBK cells with an MIC of 8 \u00b5M. In semen, viral titer was significantly reduced at 10 \u00b5M with complete inactivation at 30 \u00b5M, confirmed by the absence of cytopathic effect in MDBK cells. Sperm kinematic, structural and functional integrity analysis demonstrated that semen quality parameters were preserved at concentrations up to 30 \u00b5M, while 100 \u00b5M caused a significant reduction in total and progressive motility.<\/jats:p>","DOI":"10.1007\/s11259-026-11544-2","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:07:56Z","timestamp":1790395676000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of indolicidin for inactivation of bovine alphaherpesvirus 1 in semen"],"prefix":"10.1007","volume":"50","author":[{"given":"Jo\u00e3o Paulo Yoshio Prado Cerqueira","family":"Kubota","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Henrique","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taise Maria","family":"dos Anjos Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L\u00facia","family":"Gambarini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyla","family":"de Oliveira Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klayto Jos\u00e9 Gon\u00e7alves","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme Sastre","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Carolina Oliveira","family":"de Arruda Brasil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Maffud","family":"Cilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban Nicol\u00e1s","family":"Lorenz\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7671-5526","authenticated-orcid":false,"given":"Guilherme Rocha Lino","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,26]]},"reference":[{"key":"11544_CR12","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-26797-5_8","volume-title":"Andrological Evaluation of Male Infertility","author":"A Agarwal","year":"2016","unstructured":"Agarwal A, Gupta S, Sharma R (2016) Eosin-Nigrosin Staining Procedure. 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