{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T01:39:13Z","timestamp":1792460353231,"version":"4.5.0"},"reference-count":12,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res Commun"],"published-print":{"date-parts":[[2027,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This partially randomized crossover pilot study evaluated reduced doses of histrelin for ovulation induction in mares and compared intramuscular (IM) administration with pharmacopuncture at the\n                    <jats:italic>Bai-hui<\/jats:italic>\n                    and\n                    <jats:italic>Yan-chi<\/jats:italic>\n                    acupoints. Eleven mares were enrolled, of which nine completed all eight protocols and were included in the analyses. The protocols comprised 250\u00a0\u00b5g histrelin IM (standard dose), 75\u00a0\u00b5g IM, 25\u00a0\u00b5g IM, no intervention, saline at\n                    <jats:italic>Bai-hui<\/jats:italic>\n                    , saline at\n                    <jats:italic>Yan-chi<\/jats:italic>\n                    , 25\u00a0\u00b5g histrelin at\n                    <jats:italic>Bai-hui<\/jats:italic>\n                    , and 25\u00a0\u00b5g histrelin at\n                    <jats:italic>Yan-chi<\/jats:italic>\n                    . Ovulation was assessed by transrectal ultrasonography at scheduled examinations until detected, with ovulation within 48\u00a0h as the primary outcome. Ovulations first detected at 24\u00a0h were excluded from the primary analysis. Ovulation within 48\u00a0h occurred in 88.9% (8\/9) of observations with 250\u00a0\u00b5g IM, 87.5% (7\/8) with 75\u00a0\u00b5g IM, 87.5% (7\/8) with 25\u00a0\u00b5g IM, 37.5% (3\/8) with no intervention, 50.0% (3\/6) with saline at\n                    <jats:italic>Bai-hui<\/jats:italic>\n                    , 100% (9\/9) with 25\u00a0\u00b5g histrelin at\n                    <jats:italic>Bai-hui<\/jats:italic>\n                    , 12.5% (1\/8) with saline at\n                    <jats:italic>Yan-chi<\/jats:italic>\n                    , and 87.5% (7\/8) with 25\u00a0\u00b5g histrelin at\n                    <jats:italic>Yan-chi<\/jats:italic>\n                    . Exact 95% confidence intervals were wide, reflecting the small sample size. Exploratory pairwise comparisons with the no-intervention protocol were performed without adjustment for multiple comparisons and should be interpreted cautiously. The findings provide preliminary evidence that reduced histrelin doses, including 25\u00a0\u00b5g, may induce ovulation within 48\u00a0h in mares. However, this study did not demonstrate an advantage of pharmacopuncture over IM administration of the same reduced dose. Larger, fully randomized studies are required to confirm the efficacy of reduced-dose histrelin and determine whether administration at specific acupoints provides any additional effect.\n                  <\/jats:p>","DOI":"10.1007\/s11259-026-11583-9","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:47:27Z","timestamp":1792457247000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Low-dose histrelin for ovulation induction in mares: a partially randomized crossover pilot study"],"prefix":"10.1007","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7447-6546","authenticated-orcid":false,"given":"Lys","family":"de Barros Fogagnoli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4475-251X","authenticated-orcid":false,"given":"Kallena Canali Abdala","family":"Jose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5534-3748","authenticated-orcid":false,"given":"Rachel","family":"Weckl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4335-7116","authenticated-orcid":false,"given":"Clara Mantovani Ozorio","family":"Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0044-1694","authenticated-orcid":false,"given":"Mirelle Ochrimowicz","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7459-0507","authenticated-orcid":false,"given":"Rafaela Caroline","family":"Machado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7993-3919","authenticated-orcid":false,"given":"Carlos Eduardo","family":"Camargo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0893-1654","authenticated-orcid":false,"given":"Pedro Vicente","family":"Michelotto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,20]]},"reference":[{"key":"11583_CR1","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/S0737-0806(98)80370-5","volume":"18","author":"MA Alvarenga","year":"1998","unstructured":"Alvarenga MA, Ferreira JPC, Meira C, Luna SPL, Burns PJ (1998) Induction of luteolysis in mares utilizing a micro-dose of prostaglandin F2\u03b1 in the sacral lumbar space. J Equine Vet Sci 18:167\u2013168. https:\/\/doi.org\/10.1016\/S0737-0806(98)80370-5","journal-title":"J Equine Vet Sci"},{"key":"11583_CR2","doi-asserted-by":"publisher","first-page":"e23302","DOI":"10.7213\/acad.2025.23302","volume":"23","author":"NGH Cardoso","year":"2025","unstructured":"Cardoso NGH, Kozicki LE, Camargo CE, Rodrigues PA, Michelotto J\u00fanior PV (2025) Acupuncture-mediated ovulation induction in mares: evaluating the efficacy of subdose of histrelin at Bai-hui and Governing Vessel-1 acupoints. Rev Acad Cienc Anim 23:e23302. https:\/\/doi.org\/10.7213\/acad.2025.23302","journal-title":"Rev Acad Cienc Anim"},{"key":"11583_CR3","doi-asserted-by":"publisher","first-page":"104698","DOI":"10.1016\/j.jevs.2023.104698","volume":"125","author":"N Cazales","year":"2023","unstructured":"Cazales N, Pereyra F, Icatt S, Colm\u00e1n NJ, Camacho CA, Estrad\u00e9 MJ, Mattos RC (2023) HCG versus histrelin acetate: ovulation inducing agents in mares (Equus caballus) in a commercial breeding program in Uruguay. J Equine Vet Sci 125:104698. https:\/\/doi.org\/10.1016\/j.jevs.2023.104698","journal-title":"J Equine Vet Sci"},{"key":"11583_CR4","doi-asserted-by":"publisher","DOI":"10.1002\/9781119556015","volume-title":"Equine reproductive procedures","author":"J Dascanio","year":"2021","unstructured":"Dascanio J, McCue P (2021) Equine reproductive procedures, 2nd edn. Wiley-Blackwell, Hoboken","edition":"2"},{"key":"11583_CR5","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1111\/j.2042-3306.1983.tb01826.x","volume":"15","author":"DR Henneke","year":"1983","unstructured":"Henneke DR, Potter GD, Kreider JL, Yeats BF (1983) Relationship between condition score, physical measurements and body fat percentage in mares. Equine Vet J 15:371\u2013372. https:\/\/doi.org\/10.1111\/j.2042-3306.1983.tb01826.x","journal-title":"Equine Vet J"},{"key":"11583_CR6","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/S2005-2901(10)60029-5","volume":"3","author":"J Kim","year":"2010","unstructured":"Kim J, Kang D (2010) A descriptive statistical approach to the Korean pharmacopuncture therapy. J Acupunct Meridian Stud 3:141\u2013149. https:\/\/doi.org\/10.1016\/S2005-2901(10)60029-5","journal-title":"J Acupunct Meridian Stud"},{"key":"11583_CR7","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1016\/S0093-691X(02)00923-8","volume":"58","author":"PM McCue","year":"2002","unstructured":"McCue PM, Farquhar VJ, Carnevale EM, Squires EL (2002) Removal of deslorelin (Ovuplant) implant 48\u00a0h after administration results in normal interovulatory intervals in mares. Theriogenology 58:865\u2013870","journal-title":"Theriogenology"},{"key":"11583_CR8","doi-asserted-by":"publisher","first-page":"123","DOI":"10.58292\/CT.v11.11168","volume":"11","author":"J Morrissey","year":"2019","unstructured":"Morrissey J, Ferris R, McCue P (2019) Comparison of deslorelin and histrelin for induction of ovulation in mares. Clin Theriogenol 11:123\u2013126. https:\/\/doi.org\/10.58292\/CT.v11.11168","journal-title":"Clin Theriogenol"},{"key":"11583_CR9","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.2460\/ajvr.2001.62.1285","volume":"62","author":"GJ Nie","year":"2001","unstructured":"Nie GJ, Goodin AN, Braden TD, Wenzel JGW (2001) Luteal and clinical response following administration of dinoprost tromethamine or cloprostenol at standard intramuscular sites or at the lumbosacral acupuncture point in mares. Am J Vet Res 62:1285\u20131289. https:\/\/doi.org\/10.2460\/ajvr.2001.62.1285","journal-title":"Am J Vet Res"},{"issue":"Suppl 1","key":"11583_CR10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S1472-6483(11)60210-1","volume":"5","author":"O Ortmann","year":"2002","unstructured":"Ortmann O, Weiss JM, Diedrich K (2002) Gonadotrophin-releasing hormone (GnRH) and GnRH agonists: mechanisms of action. Reprod Biomed Online 5(Suppl 1):1\u20137. https:\/\/doi.org\/10.1016\/S1472-6483(11)60210-1","journal-title":"Reprod Biomed Online"},{"key":"11583_CR11","doi-asserted-by":"publisher","first-page":"2027","DOI":"10.1590\/1678-4162-11820","volume":"72","author":"MO Ribeiro","year":"2020","unstructured":"Ribeiro MO, Silva MAA, Maggitti Junior LDP, Bezerra PA, Bittencourt RF, Feliciano MAR, Santana ALA, Silva PDA, Barbosa LP (2020) Effect of the subdose of human chorionic gonadotropin applied in the Hou Hai acupoint on ovulation induction in mares. Arq Bras Med Vet Zootec 72:2027\u20132035. https:\/\/doi.org\/10.1590\/1678-4162-11820","journal-title":"Arq Bras Med Vet Zootec"},{"key":"11583_CR12","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1002\/9780470344569.ch4","volume-title":"Xie\u2019s veterinary acupuncture","author":"H Xie","year":"2007","unstructured":"Xie H, Yamagiwa K (2007) Equine classical acupoints. In: Xie H, Preast V (eds) Xie\u2019s veterinary acupuncture. Blackwell, Ames, pp 89\u2013127"}],"container-title":["Veterinary Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11583-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11583-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11259-026-11583-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:47:34Z","timestamp":1792457254000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11259-026-11583-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,20]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2027,1]]}},"alternative-id":["11583"],"URL":"https:\/\/doi.org\/10.1007\/s11259-026-11583-9","relation":{},"ISSN":["0165-7380","1573-7446"],"issn-type":[{"value":"0165-7380","type":"print"},{"value":"1573-7446","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,20]]},"assertion":[{"value":"11 August 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 October 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"8"}}