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Perchloroethene and trichloroethene can be dechlorinated to non-toxic ethene through reductive dechlorination by <jats:italic>Dehalococcoides<\/jats:italic> sp. Bioaugmentation, applying cultures containing organohalide-respiring microorganisms, is a possible technique to remediate sites contaminated with chlorinated ethenes. Application of site specific inocula is an efficient alternative solution. Our aim was to develop site specific dechlorinating microbial inocula by enriching microbial consortia from groundwater contaminated with trichloroethene using microcosm experiments containing clay mineral as solid phase. Our main goal was to develop fast and reliable method to produce large amount (100\u00a0L) of bioactive agent with anaerobic fermentation technology. Polyphasic approach has been applied to monitor the effectiveness of dechlorination during the transfer process from bench-scale (500\u00a0mL) to industrial-scale (100\u00a0L). Gas chromatography measurement and T-RFLP (Terminal Restriction Fragment Length Polymorphism) revealed that the serial subculture of the enrichments shortened the time-course of the complete dechlorination of trichloroethene to ethene and altered the composition of bacterial communities. Complete dechlorination was observed in enrichments with significant abundance of <jats:italic>Dehalococcoides<\/jats:italic> sp. cultivated at 8\u00a0\u00b0C. Consortia incubated in fermenters at 18\u00a0\u00b0C accelerated the conversion of TCE to ethene by 7\u201314\u00a0days. Members of the enrichments belong to the phyla Bacteroidetes, Chloroflexi, Proteobacteria and Firmicutes. According to the operational taxonomic units, main differences between the composition of the enrichment incubated at 8\u00a0\u00b0C and 18\u00a0\u00b0C occurred with relative abundance of acetogenic and fermentative species. In addition to the temperature, the site-specific origin of the microbial communities and the solid phase applied during the fermentation technique contributed to the development of a unique microbial composition.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Graphic abstract<\/jats:title>\n\n<\/jats:sec>","DOI":"10.1007\/s11274-020-2806-7","type":"journal-article","created":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T10:03:26Z","timestamp":1580724206000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Preparation and characterization of site-specific dechlorinating microbial inocula capable of complete dechlorination enriched in anaerobic microcosms amended with clay mineral"],"prefix":"10.1007","volume":"36","author":[{"given":"Zsuzsanna","family":"Nagym\u00e1t\u00e9","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Jurecska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Csaba","family":"Romsics","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanni","family":"T\u00f3th","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikt\u00f3ria","family":"B\u00f3dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9va","family":"M\u00e9sz\u00e1ros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"Szab\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bal\u00e1zs","family":"Erd\u00e9lyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e1roly","family":"M\u00e1rialigeti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,3]]},"reference":[{"issue":"7","key":"2806_CR1","doi-asserted-by":"crossref","first-page":"2318","DOI":"10.1021\/es062076m","volume":"41","author":"L Adrian","year":"2007","unstructured":"Adrian L, Hansen SK, Fung JM, G\u00f6risch H, Zinder SH (2007) Growth of Dehalococcoides strains with chlorophenols as electron acceptors. 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