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Antibiotic therapy is one of the most crucial practices to treat mastitis, although the susceptibility of caprine mastitis pathogens to current antibiotics has not been tested under standard or modified incubation conditions. This work evaluated the in vitro activity of tildipirosin, gamithromycin, oxytetracycline, and danofloxacin against caprine mastitis pathogens incubated following standard conditions of Clinical and Laboratory Standards Institute (CLSI) and deviation method by 25% supplementation with goat serum. <jats:italic>Mycoplasma agalactiae, Escherichia coli, Staphylococcus aureus<\/jats:italic>, <jats:italic>Streptococcus<\/jats:italic> spp., and coagulase-negative Staphylococci (CNS) were isolated from dairy goats with mastitis in Spain. Minimum inhibitory concentrations (MICs) were determined using the broth microdilution technique. The lowest MIC<jats:sub>90<\/jats:sub> under standard conditions was obtained with danofloxacin for mastitis-causing pathogens. An exception was <jats:italic>M. agalactiae<\/jats:italic>, where danofloxacin and oxytetracycline obtained low values. However, after adding serum, gamithromycin showed the lowest MIC<jats:sub>50<\/jats:sub> for <jats:italic>S. aureus<\/jats:italic>, <jats:italic>Streptococcus<\/jats:italic> spp., and CNS. The lowest MIC<jats:sub>50<\/jats:sub> was obtained with all the antibiotics tested (&lt;\u20090.125\u00a0\u00b5g\/ml) against <jats:italic>M. agalactiae<\/jats:italic>. Supplementing with serum resulted in a significant variation in tildipirosin and gamithromycin MIC values for CNS, <jats:italic>S. aureus<\/jats:italic>, <jats:italic>M. agalagtiae<\/jats:italic>, and <jats:italic>E. coli<\/jats:italic>. In brief, the MIC for antibiotics used against mastitis should be determined under conditions closely resembling intramammary infections to obtain representative susceptibility patterns against mastitis pathogens. Caprine mastitis pathogens were broadly susceptible to danofloxacin under standard conditions. The potency of macrolides against caprine mastitis pathogens increases when serum is present in culture media.<\/jats:p>","DOI":"10.1007\/s11274-022-03407-0","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T02:02:27Z","timestamp":1663034547000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Susceptibility of caprine mastitis pathogens to tildipirosin, gamithromycin, oxytetracycline, and danofloxacin: effect of serum on the in vitro potency of current macrolides"],"prefix":"10.1007","volume":"38","author":[{"given":"Juan Sebastian","family":"Galecio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Escudero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Carlos","family":"Corrales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar","family":"Garc\u00eda-Romero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"de la Fe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica","family":"Hernandis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Marin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,9,13]]},"reference":[{"issue":"16","key":"3407_CR1","doi-asserted-by":"publisher","first-page":"416","DOI":"10.1136\/vr.100481","volume":"170","author":"S Agnello","year":"2012","unstructured":"Agnello S, Chetta M, Vicari D et al (2012) Severe outbreaks of polyarthritis in kids caused by Mycoplasma mycoides subspecies capri in Sicily. 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