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However, the traditional antibiotic treatment of <jats:italic>H. pylori<\/jats:italic> is limited due to increased antibiotic resistance, low efficacy, and low drug concentration in the stomach. This study developed a Nano-emulsion system with ability to carry Curcumin and Clarithromycin to protect them against stomach acidity and increase their efficacy against <jats:italic>H. pylori.<\/jats:italic> We used oil in water emulsion system to prepare a novel Curcumin Clarithromycin Nano-Emulsion (Cur-CLR-NE). The nano-emulsion was validated by dynamic light scattering (DLS) technique, zeta potential; transmission electron microscopy (mean particle size 48\u00a0nm), UV\u2013visible scanning and Fourier transform infrared spectroscopy (FT-IR). The in vitro assay of Cur-CLR-NE against <jats:italic>H. pylori<\/jats:italic> was evaluated by minimum inhibitory concentration (12.5 to 6.26\u00a0\u00b5g\/mL), minimum bactericidal concentration (MBC) and anti-biofilm that showed a higher inhibitory effect of Cur-CLR-NE in compere with, free curcumin and clarithromycin against <jats:italic>H. pylori<\/jats:italic>. The in vivo results indicated that Cur-CLR-NE showed higher <jats:italic>H. pylori<\/jats:italic> clearance effect than free clarithromycin or curcumin under the same administration frequency and the same dose regimen. Histological analysis clearly showed that curcumin is highly effective in repairing damaged tissue. In addition, a potent synergistic effect was obvious between clarithromycin and curcumin in nano-emulsion system. The inflammation, superficial damage, the symptoms of gastritis including erosion in the mouse gastric mucosa, necrosis of the gastric epithelium gastric glands and interstitial oedema of tunica muscularis were observed in the positive control infected mice and absent from treated mice with Cur-CLR-NE.<\/jats:p>","DOI":"10.1007\/s11274-023-03745-7","type":"journal-article","created":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T09:02:56Z","timestamp":1695978176000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Curcumin clarithromycin nano-form a promising agent to fight Helicobacter pylori infections"],"prefix":"10.1007","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1784-101X","authenticated-orcid":false,"given":"Farag M.","family":"Mosallam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud M.","family":"Bendary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rana","family":"Elshimy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed I.","family":"El-Batal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,9,29]]},"reference":[{"key":"3745_CR1","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1007\/s11274-022-03289-2","volume":"38","author":"AM Abouwarda","year":"2022","unstructured":"Abouwarda AM, Ismail TA, Abu El-Wafa WM, Faraag AHI (2022) Synergistic activity and molecular modelling of fosfomycin combinations with some antibiotics against multidrug resistant Helicobacter pylori. 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