{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T15:50:11Z","timestamp":1786981811816,"version":"build-2736575974"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T00:00:00Z","timestamp":1709164800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T00:00:00Z","timestamp":1709164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"2020 Anhui Provincial University Cooperative Research and Public Health Collaborative Innovation Project of Anhui Provincial Department of Education","award":["GXXT-2020-016"],"award-info":[{"award-number":["GXXT-2020-016"]}]},{"name":"2020 Anhui Provincial University Cooperative Research and Public Health Collaborative Innovation Project of Anhui Provincial Department of Education","award":["GXXT-2020-016"],"award-info":[{"award-number":["GXXT-2020-016"]}]},{"name":"2020 Anhui Provincial University Cooperative Research and Public Health Collaborative Innovation Project of Anhui Provincial Department of Education","award":["GXXT-2020-016"],"award-info":[{"award-number":["GXXT-2020-016"]}]},{"name":"2020 Anhui Provincial University Cooperative Research and Public Health Collaborative Innovation Project of Anhui Provincial Department of Education","award":["GXXT-2020-016"],"award-info":[{"award-number":["GXXT-2020-016"]}]},{"name":"2020 Anhui Provincial University Cooperative Research and Public Health Collaborative Innovation Project of Anhui Provincial Department of Education","award":["GXXT-2020-016"],"award-info":[{"award-number":["GXXT-2020-016"]}]},{"name":"2020 Anhui Provincial University Cooperative Research and Public Health Collaborative Innovation Project of Anhui Provincial Department of Education","award":["GXXT-2020-016"],"award-info":[{"award-number":["GXXT-2020-016"]}]},{"name":"2020 Anhui Provincial University Cooperative Research and Public Health Collaborative Innovation Project of Anhui Provincial Department of Education","award":["GXXT-2020-016"],"award-info":[{"award-number":["GXXT-2020-016"]}]},{"name":"2020 Anhui Provincial University Cooperative Research and Public Health Collaborative Innovation Project of Anhui Provincial Department of Education","award":["GXXT-2020-016"],"award-info":[{"award-number":["GXXT-2020-016"]}]},{"name":"2021 Anhui Provincial Medical and Health Key Specialty Construction Project","award":["serial number 95"],"award-info":[{"award-number":["serial number 95"]}]},{"name":"2021 Anhui Provincial Medical and Health Key Specialty Construction Project","award":["serial number 95"],"award-info":[{"award-number":["serial number 95"]}]},{"name":"2021 Anhui Provincial Medical and Health Key Specialty Construction Project","award":["serial number 95"],"award-info":[{"award-number":["serial number 95"]}]},{"name":"2021 Anhui Provincial Medical and Health Key Specialty Construction Project","award":["serial number 95"],"award-info":[{"award-number":["serial number 95"]}]},{"name":"2021 Anhui Provincial Medical and Health Key Specialty Construction Project","award":["serial number 95"],"award-info":[{"award-number":["serial number 95"]}]},{"name":"2021 Anhui Provincial Medical and Health Key Specialty Construction Project","award":["serial number 95"],"award-info":[{"award-number":["serial number 95"]}]},{"name":"2021 Anhui Provincial Medical and Health Key Specialty Construction Project","award":["serial number 95"],"award-info":[{"award-number":["serial number 95"]}]},{"name":"2021 Anhui Provincial Medical and Health Key Specialty Construction Project","award":["serial number 95"],"award-info":[{"award-number":["serial number 95"]}]},{"name":"2021 Anhui Provincial Key Project of Natural Science Research in Colleges and Universities","award":["KJ2021ZD0032"],"award-info":[{"award-number":["KJ2021ZD0032"]}]},{"name":"2021 Anhui Provincial Key Project of Natural Science Research in Colleges and Universities","award":["KJ2021ZD0032"],"award-info":[{"award-number":["KJ2021ZD0032"]}]},{"name":"2021 Anhui Provincial Key Project of Natural Science Research in Colleges and Universities","award":["KJ2021ZD0032"],"award-info":[{"award-number":["KJ2021ZD0032"]}]},{"name":"2021 Anhui Provincial Key Project of Natural Science Research in Colleges and Universities","award":["KJ2021ZD0032"],"award-info":[{"award-number":["KJ2021ZD0032"]}]},{"name":"2021 Anhui Provincial Key Project of Natural Science Research in Colleges and Universities","award":["KJ2021ZD0032"],"award-info":[{"award-number":["KJ2021ZD0032"]}]},{"name":"2021 Anhui Provincial Key Project of Natural Science Research in Colleges and Universities","award":["KJ2021ZD0032"],"award-info":[{"award-number":["KJ2021ZD0032"]}]},{"name":"2021 Anhui Provincial Key Project of Natural Science Research in Colleges and Universities","award":["KJ2021ZD0032"],"award-info":[{"award-number":["KJ2021ZD0032"]}]},{"name":"2021 Anhui Provincial Key Project of Natural Science Research in Colleges and Universities","award":["KJ2021ZD0032"],"award-info":[{"award-number":["KJ2021ZD0032"]}]},{"name":"Research Fund of Anhui Institute of translational medicine","award":["2022zhyx-C61"],"award-info":[{"award-number":["2022zhyx-C61"]}]},{"name":"Research Fund of Anhui Institute of translational medicine","award":["2022zhyx-C61"],"award-info":[{"award-number":["2022zhyx-C61"]}]},{"name":"Research Fund of Anhui Institute of translational medicine","award":["2022zhyx-C61"],"award-info":[{"award-number":["2022zhyx-C61"]}]},{"name":"Research Fund of Anhui Institute of translational medicine","award":["2022zhyx-C61"],"award-info":[{"award-number":["2022zhyx-C61"]}]},{"name":"Research Fund of Anhui Institute of translational medicine","award":["2022zhyx-C61"],"award-info":[{"award-number":["2022zhyx-C61"]}]},{"name":"Research Fund of Anhui Institute of translational medicine","award":["2022zhyx-C61"],"award-info":[{"award-number":["2022zhyx-C61"]}]},{"name":"Research Fund of Anhui Institute of translational medicine","award":["2022zhyx-C61"],"award-info":[{"award-number":["2022zhyx-C61"]}]},{"name":"Research Fund of Anhui Institute of translational medicine","award":["2022zhyx-C61"],"award-info":[{"award-number":["2022zhyx-C61"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["World J Microbiol Biotechnol"],"published-print":{"date-parts":[[2024,4]]},"DOI":"10.1007\/s11274-024-03940-0","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T21:02:22Z","timestamp":1709154142000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Establishing a pulmonary aspergillus fumigatus infection diagnostic platform based on RPA-CRISPR-Cas12a"],"prefix":"10.1007","volume":"40","author":[{"given":"Chunhui","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runde","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoping","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jilu","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,29]]},"reference":[{"issue":"6","key":"3940_CR5","doi-asserted-by":"publisher","first-page":"e48","DOI":"10.1016\/s2213-2600(20)30237-x","volume":"8","author":"A Alanio","year":"2020","unstructured":"Alanio A, Delli\u00e8re S, Fodil S, Bretagne S, M\u00e9garbane B (2020) Prevalence of putative invasive pulmonary aspergillosis in critically ill patients with COVID-19. The Lancet. Respir Med 8(6):e48\u2013e9. https:\/\/doi.org\/10.1016\/s2213-2600(20)30237-x","journal-title":"Respir Med"},{"key":"3940_CR20","doi-asserted-by":"publisher","first-page":"39","DOI":"10.3114\/sim.2007.59.05","volume":"59","author":"SA Balajee","year":"2007","unstructured":"Balajee SA, Houbraken J, Verweij PE et al (2007) Aspergillus species identification in the clinical setting. Stud Mycol 59:39\u201346. https:\/\/doi.org\/10.3114\/sim.2007.59.05","journal-title":"Stud Mycol"},{"issue":"1","key":"3940_CR22","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1007\/s11684-017-0601-0","volume":"12","author":"M Chen","year":"2018","unstructured":"Chen M, Xu Y, Hong N et al (2018) Epidemiology of fungal infections in China. Front Med 12(1):58\u201375. https:\/\/doi.org\/10.1007\/s11684-017-0601-0","journal-title":"Front Med"},{"issue":"3","key":"3940_CR14","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1039\/c5lc01388g","volume":"16","author":"JR Choi","year":"2016","unstructured":"Choi JR, Hu J, Tang R et al (2016) An integrated paper-based sample-to-answer biosensor for nucleic acid testing at the point of care. Lab Chip 16(3):611\u2013621. https:\/\/doi.org\/10.1039\/c5lc01388g","journal-title":"Lab Chip"},{"issue":"7","key":"3940_CR15","doi-asserted-by":"publisher","first-page":"947","DOI":"10.1373\/clinchem.2015.245829","volume":"62","author":"RK Daher","year":"2016","unstructured":"Daher RK, Stewart G, Boissinot M, Bergeron MG (2016) Recombinase polymerase amplification for diagnostic applications. Clin Chem 62(7):947\u2013958. https:\/\/doi.org\/10.1373\/clinchem.2015.245829","journal-title":"Clin Chem"},{"issue":"Suppl 7","key":"3940_CR13","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1111\/imj.15591","volume":"51","author":"AP Douglas","year":"2021","unstructured":"Douglas AP, Smibert OC, Bajel A et al (2021) Consensus guidelines for the diagnosis and management of invasive aspergillosis, 2021. Intern Med J 51(Suppl 7):143\u2013176. https:\/\/doi.org\/10.1111\/imj.15591","journal-title":"Intern Med J"},{"issue":"1","key":"3940_CR1","doi-asserted-by":"publisher","first-page":"1707","DOI":"10.1038\/s41467-021-21965-z","volume":"12","author":"S Henneberg","year":"2021","unstructured":"Henneberg S, Hasenberg A, Maurer A et al (2021) Antibody-guided in vivo imaging of aspergillus fumigatus lung infections during antifungal azole treatment. Nat Commun 12(1):1707. https:\/\/doi.org\/10.1038\/s41467-021-21965-z","journal-title":"Nat Commun"},{"issue":"10","key":"3940_CR9","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1016\/s1473-3099(05)70238-3","volume":"5","author":"WW Hope","year":"2005","unstructured":"Hope WW, Walsh TJ, Denning DW (2005) Laboratory diagnosis of invasive aspergillosis. The Lancet. Infect Dis 5(10):609\u2013622. https:\/\/doi.org\/10.1016\/s1473-3099(05)70238-3","journal-title":"Infect Dis"},{"issue":"4","key":"3940_CR26","doi-asserted-by":"publisher","first-page":"e0188623","DOI":"10.1128\/spectrum.01886-23","volume":"11","author":"T Jiang","year":"2023","unstructured":"Jiang T, Hu X, Shen J (2023) Establishment of a Novel detection platform for Clostridioides difficile toxin genes based on Orthogonal CRISPR. Microbiol Spectr 11(4):e0188623. https:\/\/doi.org\/10.1128\/spectrum.01886-23","journal-title":"Microbiol Spectr"},{"key":"3940_CR2","doi-asserted-by":"publisher","unstructured":"Lamoth F, Calandra T (2022) Pulmonary aspergillosis: diagnosis and treatment. Eur Respiratory Review: Official J Eur Respiratory Soc 31(166). https:\/\/doi.org\/10.1183\/16000617.0114-2022","DOI":"10.1183\/16000617.0114-2022"},{"key":"3940_CR21","doi-asserted-by":"publisher","unstructured":"Ledoux MP, Guffroy B, Nivoix Y, Simand C, Herbrecht R (2020) Invasive pulmonary aspergillosis. Seminars in respiratory and critical care medicine. 41(1):80\u201398. https:\/\/doi.org\/10.1055\/s-0039-3401990","DOI":"10.1055\/s-0039-3401990"},{"key":"3940_CR11","doi-asserted-by":"publisher","unstructured":"Lee N, Kym D (2022) Clinical usefulness of serum (1,3)-\u03b2-D-glucan to predict invasive candidiasis in patients with severe burn trauma. Journal of microbiology, immunology, and infection\u2009=\u2009Wei Mian Yu Gan ran Za Zhi. 55(1):138\u2013146. https:\/\/doi.org\/10.1016\/j.jmii.2021.02.004","DOI":"10.1016\/j.jmii.2021.02.004"},{"issue":"5","key":"3940_CR18","doi-asserted-by":"publisher","first-page":"1772","DOI":"10.1021\/acssynbio.1c00620","volume":"11","author":"R Lei","year":"2022","unstructured":"Lei R, Li L, Wu P et al (2022) RPA\/CRISPR\/Cas12a-Based On-Site and Rapid Nucleic Acid Detection of Toxoplasma Gondii in the Environment. ACS Synth Biol 11(5):1772\u20131781. https:\/\/doi.org\/10.1021\/acssynbio.1c00620","journal-title":"ACS Synth Biol"},{"issue":"9","key":"3940_CR25","doi-asserted-by":"publisher","first-page":"5420","DOI":"10.1128\/aac.00905-16","volume":"60","author":"F Leonardelli","year":"2016","unstructured":"Leonardelli F, Macedo D, Dudiuk C, Cabeza MS, Gamarra S, Garcia-Effron G (2016) Aspergillus Fumigatus intrinsic fluconazole resistance is due to the naturally occurring T301I substitution in Cyp51Ap. Antimicrob Agents Chemother 60(9):5420\u20135426. https:\/\/doi.org\/10.1128\/aac.00905-16","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"3940_CR16","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1039\/c8an01621f","volume":"144","author":"J Li","year":"2018","unstructured":"Li J, Macdonald J, von Stetten F (2018) Review: a comprehensive summary of a decade development of the recombinase polymerase amplification. Analyst 144(1):31\u201367. https:\/\/doi.org\/10.1039\/c8an01621f","journal-title":"Analyst"},{"issue":"2","key":"3940_CR4","doi-asserted-by":"publisher","first-page":"e0406822","DOI":"10.1128\/spectrum.04068-22","volume":"11","author":"D Lupt\u00e1kov\u00e1","year":"2023","unstructured":"Lupt\u00e1kov\u00e1 D, Patil RH, Dobi\u00e1\u0161 R et al (2023) Siderophore-based noninvasive differentiation of aspergillus fumigatus colonization and Invasion in Pulmonary aspergillosis. Microbiol Spectr 11(2):e0406822. https:\/\/doi.org\/10.1128\/spectrum.04068-22","journal-title":"Microbiol Spectr"},{"issue":"4","key":"3940_CR12","doi-asserted-by":"publisher","first-page":"732","DOI":"10.4103\/ijpm.Ijpm_985_20","volume":"64","author":"S Malhotra","year":"2021","unstructured":"Malhotra S, Kumari R, Chauhan AK et al (2021) Diagnostic value of galactomannan antigen test in serum and bronchoalveolar lavage fluid sample from suspected patients of invasive pulmonary aspergillosis. Indian J Pathol Microbiol 64(4):732\u2013734. https:\/\/doi.org\/10.4103\/ijpm.Ijpm_985_20","journal-title":"Indian J Pathol Microbiol"},{"issue":"6","key":"3940_CR23","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1164\/rccm.200401-093OC","volume":"170","author":"W Meersseman","year":"2004","unstructured":"Meersseman W, Vandecasteele SJ, Wilmer A, Verbeken E, Peetermans WE, Van Wijngaerden E (2004) Invasive aspergillosis in critically ill patients without malignancy. Am J Respir Crit Care Med 170(6):621\u2013625. https:\/\/doi.org\/10.1164\/rccm.200401-093OC","journal-title":"Am J Respir Crit Care Med"},{"key":"3940_CR3","doi-asserted-by":"publisher","unstructured":"Moura S, Cerqueira L, Almeida A (2018) Invasive pulmonary aspergillosis: current diagnostic methodologies and a new molecular approach. European journal of clinical microbiology & infectious diseases: official publication of the European Society of Clinical Microbiology. 37(8):1393\u20131403. https:\/\/doi.org\/10.1007\/s10096-018-3251-5","DOI":"10.1007\/s10096-018-3251-5"},{"key":"3940_CR7","doi-asserted-by":"publisher","unstructured":"Pfister J, Summer D, Petrik M et al (2020) Hybrid imaging of Aspergillus Fumigatus Pulmonary infection with fluorescent, (68)Ga-Labelled siderophores. Biomolecules 10(2). https:\/\/doi.org\/10.3390\/biom10020168","DOI":"10.3390\/biom10020168"},{"key":"3940_CR6","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.mmcr.2020.05.001","volume":"31","author":"J Prattes","year":"2021","unstructured":"Prattes J, Valentin T, Hoenigl M, Talakic E, Reisinger AC, Eller P (2021) Invasive pulmonary aspergillosis complicating COVID-19 in the ICU - a case report. Med Mycol case Rep 31:2\u20135. https:\/\/doi.org\/10.1016\/j.mmcr.2020.05.001","journal-title":"Med Mycol case Rep"},{"issue":"12","key":"3940_CR8","doi-asserted-by":"publisher","first-page":"1507","DOI":"10.1086\/591531","volume":"47","author":"L Slobbe","year":"2008","unstructured":"Slobbe L, Polinder S, Doorduijn JK et al (2008) Outcome and medical costs of patients with invasive aspergillosis and acute myelogenous leukemia-myelodysplastic syndrome treated with intensive chemotherapy: an observational study. Clin Infect Dis 47(12):1507\u20131512. https:\/\/doi.org\/10.1086\/591531","journal-title":"Clin Infect Dis"},{"key":"3940_CR10","doi-asserted-by":"publisher","unstructured":"Soubani AO, Khanchandani G, Ahmed HP (2004) Clinical significance of lower respiratory tract aspergillus culture in elderly hospitalized patients. European journal of clinical microbiology & infectious diseases: official publication of the European Society of Clinical Microbiology. 23(6):491\u2013494. https:\/\/doi.org\/10.1007\/s10096-004-1137-1","DOI":"10.1007\/s10096-004-1137-1"},{"issue":"3","key":"3940_CR24","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1086\/525258","volume":"46","author":"TJ Walsh","year":"2008","unstructured":"Walsh TJ, Anaissie EJ, Denning DW et al (2008) Treatment of aspergillosis: clinical practice guidelines of the Infectious Diseases Society of America. Clin Infect Dis 46(3):327\u2013360. https:\/\/doi.org\/10.1086\/525258","journal-title":"Clin Infect Dis"},{"issue":"12","key":"3940_CR17","doi-asserted-by":"publisher","first-page":"e3000978","DOI":"10.1371\/journal.pbio.3000978","volume":"18","author":"D Xiong","year":"2020","unstructured":"Xiong D, Dai W, Gong J et al (2020) Rapid detection of SARS-CoV-2 with CRISPR-Cas12a. PLoS Biol 18(12):e3000978. https:\/\/doi.org\/10.1371\/journal.pbio.3000978","journal-title":"PLoS Biol"},{"issue":"6","key":"3940_CR19","doi-asserted-by":"publisher","first-page":"3476","DOI":"10.1021\/acs.analchem.2c05320","volume":"95","author":"Y Zhao","year":"2023","unstructured":"Zhao Y, Chen D, Xu Z et al (2023) Integrating CRISPR-Cas12a into a Microfluidic Dual-Droplet device enables simultaneous detection of HPV16 and HPV18. Anal Chem 95(6):3476\u20133485. https:\/\/doi.org\/10.1021\/acs.analchem.2c05320","journal-title":"Anal Chem"}],"container-title":["World Journal of Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-024-03940-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11274-024-03940-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-024-03940-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,6]],"date-time":"2024-04-06T05:11:03Z","timestamp":1712380263000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11274-024-03940-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,29]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["3940"],"URL":"https:\/\/doi.org\/10.1007\/s11274-024-03940-0","relation":{},"ISSN":["0959-3993","1573-0972"],"issn-type":[{"value":"0959-3993","type":"print"},{"value":"1573-0972","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,29]]},"assertion":[{"value":"17 December 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 February 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 February 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study involved the anonymous use of excess, discarded BAL, clinical fungal isolate samples as part of standard care, and the study did not affect patient health and privacy. All procedures were performed in accordance with the ethical standards of medical ethics in research involving human subjects, the Fourth Affiliated Hospital Committee of Anhui Medical University (reference number: LLSC20210802) and the 1964 Helsinki Declaration and its later amendments or similar ethical standards.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interest"}}],"article-number":"116"}}