{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:05:39Z","timestamp":1790222739722,"version":"4.1.0"},"reference-count":73,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"DST-SERB","award":["SRG\/2021\/000293"],"award-info":[{"award-number":["SRG\/2021\/000293"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["World J Microbiol Biotechnol"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1007\/s11274-025-04441-4","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T22:49:58Z","timestamp":1751582998000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["High-throughput screening identifies novel chemical scaffolds targeting Leishmania donovani parasites"],"prefix":"10.1007","volume":"41","author":[{"given":"Diksha","family":"Kumari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishwani","family":"Jamwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tashi","family":"Palmo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuj","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuljit","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,4]]},"reference":[{"issue":"1","key":"4441_CR1","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1186\/s12894-024-01521-9","volume":"24","author":"BO Ajiboye","year":"2024","unstructured":"Ajiboye BO, Fatoki TH, Akinola OG et al (2024) In silico exploration of anti-prostate cancer compounds from differential expressed genes. BMC Urol 24(1):138. https:\/\/doi.org\/10.1186\/s12894-024-01521-9","journal-title":"BMC Urol"},{"issue":"3","key":"4441_CR2","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1039\/d0md00353k","volume":"12","author":"Y Akao","year":"2021","unstructured":"Akao Y, Canan S, Cao Y et al (2021) Collaborative virtual screening to elaborate an imidazo[1,2-a]pyridine hit series for visceral leishmaniasis. RSC Med Chem 12(3):384\u2013393. https:\/\/doi.org\/10.1039\/d0md00353k","journal-title":"RSC Med Chem"},{"key":"4441_CR3","doi-asserted-by":"publisher","unstructured":"Aldewachi H, Al-Zidan RN, Conner MT, Salman MM (2021) High-throughput screening platforms in the discovery of novel drugs for neurodegenerative diseases. Bioengineering (Basel) 8(2). https:\/\/doi.org\/10.3390\/bioengineering8020030","DOI":"10.3390\/bioengineering8020030"},{"key":"4441_CR4","doi-asserted-by":"publisher","unstructured":"\u00c1lvarez-Bard\u00f3n M, P\u00e9rez-Pertejo Y, Ord\u00f3\u00f1ez C et al (2020) Screening marine natural products for new drug leads against trypanosomatids and malaria. Mar Drugs 18(4). https:\/\/doi.org\/10.3390\/md18040187","DOI":"10.3390\/md18040187"},{"issue":"1","key":"4441_CR5","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1177\/1087057114555846","volume":"20","author":"F Annang","year":"2015","unstructured":"Annang F, P\u00e9rez-Moreno G, Garc\u00eda-Hern\u00e1ndez R et al (2015) High-throughput screening platform for natural product-based drug discovery against 3 neglected tropical diseases: human African trypanosomiasis, leishmaniasis, and Chagas disease. J Biomol Screen 20(1):82\u201391. https:\/\/doi.org\/10.1177\/1087057114555846","journal-title":"J Biomol Screen"},{"key":"4441_CR6","doi-asserted-by":"publisher","unstructured":"Ayon NJ (2023) High-throughput screening of natural product and synthetic molecule libraries for antibacterial drug discovery. Metabolites 13(5). https:\/\/doi.org\/10.3390\/metabo13050625","DOI":"10.3390\/metabo13050625"},{"issue":"4","key":"4441_CR7","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1093\/jac\/dkz515","volume":"75","author":"R Bansal","year":"2020","unstructured":"Bansal R, Sen SS, Muthuswami R, Madhubala R (2020) Stigmasterol as a potential biomarker for amphotericin B resistance in Leishmania donovani. J Antimicrob Chemother 75(4):942\u2013950. https:\/\/doi.org\/10.1093\/jac\/dkz515","journal-title":"J Antimicrob Chemother"},{"key":"4441_CR8","doi-asserted-by":"publisher","unstructured":"Bhattacharya A, Corbeil A, do Monte-Neto RL, Fernandez-Prada C (2020) Of drugs and trypanosomatids: New tools and knowledge to reduce bottlenecks in drug discovery. Genes (Basel) 11(7). https:\/\/doi.org\/10.3390\/genes11070722","DOI":"10.3390\/genes11070722"},{"issue":"10","key":"4441_CR9","doi-asserted-by":"publisher","first-page":"1807","DOI":"10.1016\/j.drudis.2020.07.024","volume":"25","author":"V Blay","year":"2020","unstructured":"Blay V, Tolani B, Ho SP, Arkin MR (2020) High-Throughput Screening: today\u2019s biochemical and cell-based approaches. Drug Discov Today 25(10):1807\u20131821. https:\/\/doi.org\/10.1016\/j.drudis.2020.07.024","journal-title":"Drug Discov Today"},{"issue":"4","key":"4441_CR10","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1021\/acscentsci.3c01517","volume":"10","author":"D Boldini","year":"2024","unstructured":"Boldini D, Friedrich L, Kuhn D, Sieber SA (2024) Machine learning assisted hit prioritization for high throughput screening in drug discovery. ACS Cent Sci 10(4):823\u2013832. https:\/\/doi.org\/10.1021\/acscentsci.3c01517","journal-title":"ACS Cent Sci"},{"issue":"1","key":"4441_CR11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12866-014-0236-0","volume":"14","author":"EA Britta","year":"2014","unstructured":"Britta EA, Scariot DB, Falzirolli H et al (2014) Cell death and ultrastructural alterations in Leishmania amazonensis caused by new compound 4-Nitrobenzaldehyde thiosemicarbazone derived from S-limonene. BMC Microbiol 14(1):1\u201312","journal-title":"BMC Microbiol"},{"key":"4441_CR12","doi-asserted-by":"publisher","first-page":"1379518","DOI":"10.3389\/fchem.2024.1379518","volume":"12","author":"B Chan","year":"2024","unstructured":"Chan B, Lynch NB, Tran W et al (2024) Fragment-based drug discovery for disorders of the central nervous system: designing better drugs piece by piece. Front Chem 12:1379518. https:\/\/doi.org\/10.3389\/fchem.2024.1379518","journal-title":"Front Chem"},{"key":"4441_CR13","doi-asserted-by":"publisher","first-page":"e12813","DOI":"10.7717\/peerj.12813","volume":"10","author":"W Chanmol","year":"2022","unstructured":"Chanmol W, Siriyasatien P, Intakhan N (2022) In vitro anti-Leishmania activity of 8-hydroxyquinoline and its synergistic effect with amphotericin B deoxycholate against Leishmania martiniquensis. PeerJ 10:e12813. https:\/\/doi.org\/10.7717\/peerj.12813","journal-title":"PeerJ"},{"issue":"2","key":"4441_CR14","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1021\/acsinfecdis.4c00431","volume":"11","author":"R Chaturvedi","year":"2025","unstructured":"Chaturvedi R, Sharma A (2025) Key facets for the elimination of vector-borne diseases Filariasis, Leishmaniasis, and malaria. ACS Infectious Diseases 11(2):287\u2013304","journal-title":"ACS Infectious Diseases"},{"issue":"4","key":"4441_CR15","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1016\/j.ijantimicag.2019.07.013","volume":"54","author":"HN Corman","year":"2019","unstructured":"Corman HN, Shoue DA, Norris-Mullins B, Melancon BJ, Morales MA, McDowell MA (2019) Development of a target-free high-throughput screening platform for the discovery of antileishmanial compounds. Int J Antimicrob Agents 54(4):496\u2013501. https:\/\/doi.org\/10.1016\/j.ijantimicag.2019.07.013","journal-title":"Int J Antimicrob Agents"},{"issue":"4","key":"4441_CR16","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1080\/20477724.2020.1754655","volume":"114","author":"S Cortes","year":"2020","unstructured":"Cortes S, Bruno de Sousa C, Morais T, Lago J, Campino L (2020) Potential of the natural products against leishmaniasis in Old World - a review of in-vitro studies. Pathog Glob Health 114(4):170\u2013182. https:\/\/doi.org\/10.1080\/20477724.2020.1754655","journal-title":"Pathog Glob Health"},{"issue":"5","key":"4441_CR17","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1080\/17460441.2017.1308351","volume":"12","author":"GE Croston","year":"2017","unstructured":"Croston GE (2017) The utility of target-based discovery. Expert Opin Drug Discov 12(5):427\u2013429. https:\/\/doi.org\/10.1080\/17460441.2017.1308351","journal-title":"Expert Opin Drug Discov"},{"issue":"1","key":"4441_CR18","doi-asserted-by":"publisher","first-page":"e0050923","DOI":"10.1128\/aac.00509-23","volume":"68","author":"M Cruz","year":"2024","unstructured":"Cruz M, Santi AMM, Morais-Teixeira E et al (2024) Anti-Leishmania compounds can be screened using Leishmania spp. expressing red fluorescence (tdTomato). Antimicrob Agents Chemother 68(1):e0050923. https:\/\/doi.org\/10.1128\/aac.00509-23","journal-title":"Antimicrob Agents Chemother"},{"key":"4441_CR19","doi-asserted-by":"publisher","first-page":"e210402","DOI":"10.1590\/0074-02760210402","volume":"117","author":"RF Dantas","year":"2022","unstructured":"Dantas RF, Torres-Santos EC, Silva FP Jr (2022) Past and future of trypanosomatids high-throughput phenotypic screening. Mem Inst Oswaldo Cruz 117:e210402. https:\/\/doi.org\/10.1590\/0074-02760210402","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1","key":"4441_CR20","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1038\/s41579-022-00777-y","volume":"21","author":"M De Rycker","year":"2023","unstructured":"De Rycker M, Wyllie S, Horn D, Read KD, Gilbert IH (2023) Anti-trypanosomatid drug discovery: progress and challenges. Nat Rev Microbiol 21(1):35\u201350. https:\/\/doi.org\/10.1038\/s41579-022-00777-y","journal-title":"Nat Rev Microbiol"},{"issue":"1","key":"4441_CR21","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1017\/s003118201300142x","volume":"141","author":"R Don","year":"2014","unstructured":"Don R, Ioset JR (2014) Screening strategies to identify new chemical diversity for drug development to treat kinetoplastid infections. Parasitology 141(1):140\u2013146. https:\/\/doi.org\/10.1017\/s003118201300142x","journal-title":"Parasitology"},{"key":"4441_CR22","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/164_2015_20","volume":"232","author":"J Eder","year":"2016","unstructured":"Eder J, Herrling PL (2016) Trends in modern drug discovery. Handb Exp Pharmacol 232:3\u201322. https:\/\/doi.org\/10.1007\/164_2015_20","journal-title":"Handb Exp Pharmacol"},{"issue":"1","key":"4441_CR23","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1021\/acsinfecdis.4c00327","volume":"11","author":"MA Elfawal","year":"2025","unstructured":"Elfawal MA, Goetz E, Kim Y et al (2025) High-throughput screening of more than 30,000 compounds for anthelmintics against gastrointestinal nematode parasites. ACS Infect Dis 11(1):104\u2013120. https:\/\/doi.org\/10.1021\/acsinfecdis.4c00327","journal-title":"ACS Infect Dis"},{"issue":"2","key":"4441_CR24","doi-asserted-by":"publisher","first-page":"242","DOI":"10.2741\/e820","volume":"10","author":"RO Eren","year":"2018","unstructured":"Eren RO, Kopelyanskiy D, Moreau D et al (2018) Development of a semi-automated image-based high-throughput drug screening system. Front Biosci (Elite Ed) 10(2):242\u2013253. https:\/\/doi.org\/10.2741\/e820","journal-title":"Front Biosci (Elite Ed)"},{"issue":"2","key":"4441_CR25","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/j.ijpddr.2018.03.004","volume":"8","author":"C Fernandez-Prada","year":"2018","unstructured":"Fernandez-Prada C, Sharma M, Plourde M et al (2018) High-throughput Cos-Seq screen with intracellular Leishmania infantum for the discovery of novel drug-resistance mechanisms. Int J Parasitol Drugs Drug Resist 8(2):165\u2013173. https:\/\/doi.org\/10.1016\/j.ijpddr.2018.03.004","journal-title":"Int J Parasitol Drugs Drug Resist"},{"key":"4441_CR26","doi-asserted-by":"publisher","first-page":"109591","DOI":"10.1016\/j.intimp.2022.109591","volume":"114","author":"B Gopu","year":"2023","unstructured":"Gopu B, Kour P, Pandian R, Singh K (2023) Insights into the drug screening approaches in leishmaniasis. Int Immunopharmacol 114:109591","journal-title":"Int Immunopharmacol"},{"issue":"2","key":"4441_CR27","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1021\/acs.jmedchem.9b01343","volume":"63","author":"A Harupa","year":"2020","unstructured":"Harupa A, De Las HL, Colmenarejo G et al (2020) Identification of Selective Inhibitors of Plasmodium N-Myristoyltransferase by High-Throughput Screening. J Med Chem 63(2):591\u2013600. https:\/\/doi.org\/10.1021\/acs.jmedchem.9b01343","journal-title":"J Med Chem"},{"issue":"10","key":"4441_CR28","doi-asserted-by":"publisher","first-page":"2743","DOI":"10.1007\/s00436-019-06443-2","volume":"118","author":"S Hendrickx","year":"2019","unstructured":"Hendrickx S, Caljon G, Maes L (2019) Need for sustainable approaches in antileishmanial drug discovery. Parasitol Res 118(10):2743\u20132752. https:\/\/doi.org\/10.1007\/s00436-019-06443-2","journal-title":"Parasitol Res"},{"key":"4441_CR29","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/j.mex.2018.12.003","volume":"6","author":"R Jain","year":"2019","unstructured":"Jain R, Garg S, Singh S (2019) Screening of chemical library against whole cell kinome activity via non-radioactive, high throughput kinase assay. MethodsX 6:162\u2013168. https:\/\/doi.org\/10.1016\/j.mex.2018.12.003","journal-title":"MethodsX"},{"issue":"3","key":"4441_CR30","doi-asserted-by":"publisher","first-page":"1439","DOI":"10.1007\/s11686-024-00899-8","volume":"69","author":"H Khan","year":"2024","unstructured":"Khan H, Hakami MA, Alamri MA et al (2024) Identification of novel antileishmanial chemotypes by high-throughput virtual and In Vitro screening. Acta Parasitol 69(3):1439\u20131457. https:\/\/doi.org\/10.1007\/s11686-024-00899-8","journal-title":"Acta Parasitol"},{"issue":"10","key":"4441_CR31","doi-asserted-by":"publisher","first-page":"2100","DOI":"10.1039\/D3MD00214D","volume":"14","author":"P Kour","year":"2023","unstructured":"Kour P, Saha P, Bhattacharya S et al (2023) Design, Synthesis, and biological evaluation of 3, 3\u2019-diindolylmethane N-linked glycoconjugate as Leishmanial Topoisomerase IB inhibitor with reduced cytotoxicity. RSC Med Chem 14(10):2100\u20132114","journal-title":"RSC Med Chem"},{"issue":"21","key":"4441_CR32","first-page":"e3410","volume":"9","author":"OS Koutsoni","year":"2019","unstructured":"Koutsoni OS, Karampetsou K, Dotsika E (2019) In vitro screening of antileishmanial activity of natural product compounds: Determination of IC50, CC50 and SI values. Bio-Protoc 9(21):e3410\u2013e3410","journal-title":"Bio-Protoc"},{"key":"4441_CR33","doi-asserted-by":"publisher","first-page":"107338","DOI":"10.1016\/j.actatropica.2024.107338","volume":"258","author":"D Kumari","year":"2024","unstructured":"Kumari D, Jamwal V, Singh A et al (2024a) Repurposing FDA approved drugs against Sterol C-24 methyltransferase of Leishmania donovani: A dual in silico and in vitro approach. Acta Trop 258:107338. https:\/\/doi.org\/10.1016\/j.actatropica.2024.107338","journal-title":"Acta Trop"},{"key":"4441_CR34","doi-asserted-by":"publisher","unstructured":"Kumari D, Kaur H, Palmo T, Nargotra A, Singh K (2024b) Exploring natural product library as potential target against sterol C-24 methyltransferase protein of Leishmania donovani. Nat Prod Res 1\u20137. https:\/\/doi.org\/10.1080\/14786419.2024.2392749","DOI":"10.1080\/14786419.2024.2392749"},{"key":"4441_CR35","doi-asserted-by":"publisher","first-page":"132034","DOI":"10.1016\/j.ijbiomac.2024.132034","volume":"269","author":"D Kumari","year":"2024","unstructured":"Kumari D, Kour P, Singh CP et al (2024c) Anhydroparthenin as a dual-target inhibitor against Sterol C-24 methyltransferase and Sterol 14-\u03b1 demethylase of Leishmania donovani: A comprehensive in vitro and in silico study. Int J Biol Macromol 269:132034","journal-title":"Int J Biol Macromol"},{"key":"4441_CR36","doi-asserted-by":"publisher","first-page":"120829","DOI":"10.1016\/j.lfs.2022.120829","volume":"306","author":"D Kumari","year":"2022","unstructured":"Kumari D, Mahajan S, Kour P, Singh K (2022) Virulence factors of Leishmania parasite: Their paramount importance in unraveling novel vaccine candidates and therapeutic targets. Life Sci 306:120829","journal-title":"Life Sci"},{"key":"4441_CR37","doi-asserted-by":"publisher","first-page":"107473","DOI":"10.1016\/j.actatropica.2024.107473","volume":"260","author":"D Kumari","year":"2024","unstructured":"Kumari D, Palmo T, Mujwar S, Singh K (2024d) Harnessing computational and experimental approaches to identify potent hits against Leishmania donovani sterol C-24 methyltransferase from ChemBridge library. Acta Trop 260:107473. https:\/\/doi.org\/10.1016\/j.actatropica.2024.107473","journal-title":"Acta Trop"},{"key":"4441_CR38","doi-asserted-by":"publisher","first-page":"174436","DOI":"10.1016\/j.ejphar.2021.174436","volume":"910","author":"D Kumari","year":"2021","unstructured":"Kumari D, Perveen S, Sharma R, Singh K (2021) Advancement in leishmaniasis diagnosis and therapeutics: An update. Eur J Pharmacol 910:174436","journal-title":"Eur J Pharmacol"},{"key":"4441_CR39","doi-asserted-by":"publisher","first-page":"108400","DOI":"10.1016\/j.intimp.2021.108400","volume":"102","author":"D Kumari","year":"2022","unstructured":"Kumari D, Singh K (2022) Exploring the paradox of defense between host and Leishmania parasite. Int Immunopharmacol 102:108400","journal-title":"Int Immunopharmacol"},{"issue":"1","key":"4441_CR40","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1038\/s41598-018-36944-6","volume":"9","author":"S Lamotte","year":"2019","unstructured":"Lamotte S, Aulner N, Sp\u00e4th GF, Prina E (2019) Discovery of novel hit compounds with broad activity against visceral and cutaneous Leishmania species by comparative phenotypic screening. Sci Rep 9(1):438. https:\/\/doi.org\/10.1038\/s41598-018-36944-6","journal-title":"Sci Rep"},{"key":"4441_CR41","doi-asserted-by":"publisher","unstructured":"Lee SM, Kim MS, Hayat F, Shin D (2019) Recent Advances in the Discovery of Novel Antiprotozoal Agents. Molecules 24(21). https:\/\/doi.org\/10.3390\/molecules24213886","DOI":"10.3390\/molecules24213886"},{"issue":"1\u20133","key":"4441_CR42","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/s0169-409x(00)00129-0","volume":"46","author":"CA Lipinski","year":"2001","unstructured":"Lipinski CA, Lombardo F, Dominy BW, Feeney PJ (2001) Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Adv Drug Deliv Rev 46(1\u20133):3\u201326. https:\/\/doi.org\/10.1016\/s0169-409x(00)00129-0","journal-title":"Adv Drug Deliv Rev"},{"key":"4441_CR43","doi-asserted-by":"publisher","first-page":"876021","DOI":"10.4061\/2011\/876021","volume":"2011","author":"STd Macedo-Silva","year":"2011","unstructured":"Macedo-Silva STd, Oliveira Silva TL, Urbina JA, Souza Wd, Rodrigues JCF (2011) Antiproliferative, ultrastructural, and physiological effects of amiodarone on promastigote and amastigote forms of Leishmania amazonensis. Mol Biol Int 2011:876021","journal-title":"Mol Biol Int"},{"issue":"2","key":"4441_CR44","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/s40475-021-00232-7","volume":"8","author":"S Mann","year":"2021","unstructured":"Mann S, Frasca K, Scherrer S et al (2021) A review of leishmaniasis: current knowledge and future directions. Curr Trop Med Rep 8(2):121\u2013132","journal-title":"Curr Trop Med Rep"},{"issue":"11","key":"4441_CR45","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.2174\/1389450121666200422083735","volume":"21","author":"R Mansuri","year":"2020","unstructured":"Mansuri R, Singh J, Diwan A (2020) An insight into the current perspective and potential drug targets for visceral leishmaniasis (VL). Curr Drug Targets 21(11):1105\u20131129","journal-title":"Curr Drug Targets"},{"issue":"5","key":"4441_CR46","doi-asserted-by":"publisher","first-page":"508","DOI":"10.37349\/eds.2024.00059","volume":"2","author":"T Masne","year":"2024","unstructured":"Masne T, Kumar D, Bansode D (2024) A review of leishmaniasis: current knowledge and future directions of heterocyclic molecules. EDS 2(5):508\u2013539","journal-title":"EDS"},{"issue":"2","key":"4441_CR47","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1002\/ddr.21907","volume":"83","author":"P Mazire","year":"2022","unstructured":"Mazire P, Agarwal V, Roy A (2022) Road-map of pre-clinical treatment for Visceral Leishmaniasis. Drug Dev Res 83(2):317\u2013327. https:\/\/doi.org\/10.1002\/ddr.21907","journal-title":"Drug Dev Res"},{"issue":"2","key":"4441_CR48","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1007\/s40291-024-00762-6","volume":"29","author":"S Mehrotra","year":"2025","unstructured":"Mehrotra S, Tiwari R, Kumar R, Sundar S (2025) Advances and challenges in the diagnosis of Leishmaniasis. Mol Diagn Ther 29(2):195\u2013212. https:\/\/doi.org\/10.1007\/s40291-024-00762-6","journal-title":"Mol Diagn Ther"},{"key":"4441_CR49","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/j.freeradbiomed.2024.01.039","volume":"213","author":"A Nawaz","year":"2024","unstructured":"Nawaz A, Priya B, Singh K, Ali V (2024) Unveiling the role of serine o-acetyltransferase in drug resistance and oxidative stress tolerance in Leishmania donovani through the regulation of thiol-based redox metabolism. Free Radic Biol Med 213:371\u2013393. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2024.01.039","journal-title":"Free Radic Biol Med"},{"key":"4441_CR50","doi-asserted-by":"publisher","first-page":"109753","DOI":"10.1016\/j.compbiomed.2025.109753","volume":"188","author":"A Nawaz","year":"2025","unstructured":"Nawaz A, Rai GP, Singh K, Shanker A, Ali V (2025) Computational approaches and experimental investigation for identification of potential inhibitors targeting cysteine synthase in Leishmaniadonovani. Comput Biol Med 188:109753. https:\/\/doi.org\/10.1016\/j.compbiomed.2025.109753","journal-title":"Comput Biol Med"},{"issue":"2","key":"4441_CR51","doi-asserted-by":"publisher","first-page":"213","DOI":"10.4014\/jmb.1508.08007","volume":"26","author":"G Nierode","year":"2016","unstructured":"Nierode G, Kwon PS, Dordick JS, Kwon SJ (2016) Cell-based assay design for high-content screening of drug candidates. J Microbiol Biotechnol 26(2):213\u2013225. https:\/\/doi.org\/10.4014\/jmb.1508.08007","journal-title":"J Microbiol Biotechnol"},{"key":"4441_CR52","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.actatropica.2015.12.016","volume":"155","author":"JH No","year":"2016","unstructured":"No JH (2016) Visceral leishmaniasis: Revisiting current treatments and approaches for future discoveries. Acta Trop 155:113\u2013123","journal-title":"Acta Trop"},{"issue":"7","key":"4441_CR53","doi-asserted-by":"publisher","first-page":"1606","DOI":"10.1021\/acs.accounts.6b00631","volume":"50","author":"J Okombo","year":"2017","unstructured":"Okombo J, Chibale K (2017) Insights into integrated lead generation and target identification in malaria and tuberculosis drug discovery. Acc Chem Res 50(7):1606\u20131616. https:\/\/doi.org\/10.1021\/acs.accounts.6b00631","journal-title":"Acc Chem Res"},{"key":"4441_CR54","doi-asserted-by":"publisher","first-page":"8771","DOI":"10.1038\/srep08771","volume":"5","author":"I Pe\u00f1a","year":"2015","unstructured":"Pe\u00f1a I, Pilar Manzano M, Cantizani J et al (2015) New compound sets identified from high throughput phenotypic screening against three kinetoplastid parasites: an open resource. Sci Rep 5:8771. https:\/\/doi.org\/10.1038\/srep08771","journal-title":"Sci Rep"},{"key":"4441_CR55","doi-asserted-by":"publisher","first-page":"e00143","DOI":"10.1016\/j.parepi.2020.e00143","volume":"9","author":"A Phumee","year":"2020","unstructured":"Phumee A, Jariyapan N, Chusri S et al (2020) Determination of anti-leishmanial drugs efficacy against Leishmania martiniquensis using a colorimetric assay. Parasite Epidemiol Control 9:e00143","journal-title":"Parasite Epidemiol Control"},{"issue":"7","key":"4441_CR56","doi-asserted-by":"publisher","first-page":"e0047724","DOI":"10.1128\/mbio.00477-24","volume":"15","author":"M Queffeulou","year":"2024","unstructured":"Queffeulou M, Leprohon P, Fernandez-Prada C, Ouellette M, Mej\u00eda-Jaramillo AM (2024) CRISPR-Cas9 high-throughput screening to study drug resistance in Leishmania infantum. mBio 15(7):e0047724. https:\/\/doi.org\/10.1128\/mbio.00477-24","journal-title":"mBio"},{"key":"4441_CR57","doi-asserted-by":"publisher","first-page":"138105","DOI":"10.1016\/j.molstruc.2024.138105","volume":"1308","author":"D Rani","year":"2024","unstructured":"Rani D, Kumari D, Bhushan A et al (2024) Design, synthesis, and biological evaluation of eugenol-isoxazoline hybrid derivatives as potential anti-leishmanial agents. J Mol Struct 1308:138105","journal-title":"J Mol Struct"},{"key":"4441_CR58","doi-asserted-by":"publisher","first-page":"1527946","DOI":"10.3389\/fchem.2024.1527946","volume":"12","author":"AH Romero","year":"2025","unstructured":"Romero AH, Delgado F (2025) 4-Aminoquinoline as a privileged scaffold for the design of leishmanicidal agents: structure\u2013property relationships and key biological targets. Front Chem 12:1527946","journal-title":"Front Chem"},{"key":"4441_CR59","doi-asserted-by":"publisher","unstructured":"Ronzetti M, Simeonov A (2025) A comprehensive update on the application of high-throughput fluorescence imaging for novel drug discovery. Expert Opin Drug Discov 1\u201313. https:\/\/doi.org\/10.1080\/17460441.2025.2499123","DOI":"10.1080\/17460441.2025.2499123"},{"key":"4441_CR60","doi-asserted-by":"publisher","first-page":"102863","DOI":"10.1016\/j.parint.2024.102863","volume":"100","author":"SY Sheikh","year":"2024","unstructured":"Sheikh SY, Hassan F, Shukla D et al (2024) A review on potential therapeutic targets for the treatment of leishmaniasis. Parasitol Int 100:102863. https:\/\/doi.org\/10.1016\/j.parint.2024.102863","journal-title":"Parasitol Int"},{"key":"4441_CR61","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/j.redox.2017.03.004","volume":"12","author":"K Singh","year":"2017","unstructured":"Singh K, Ali V, Singh KP et al (2017) Deciphering the interplay between cysteine synthase and thiol cascade proteins in modulating Amphotericin B resistance and survival of Leishmania donovani under oxidative stress. Redox Biol 12:350\u2013366","journal-title":"Redox Biol"},{"issue":"9","key":"4441_CR62","doi-asserted-by":"publisher","first-page":"897","DOI":"10.2174\/1389200217666160819161444","volume":"17","author":"K Singh","year":"2016","unstructured":"Singh K, Garg G, Ali V (2016a) Current therapeutics, their problems and thiol metabolism as potential drug targets in leishmaniasis. Curr Drug Metab 17(9):897\u2013919","journal-title":"Curr Drug Metab"},{"key":"4441_CR63","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.biochi.2016.09.004","volume":"131","author":"K Singh","year":"2016","unstructured":"Singh K, Singh KP, Equbal A et al (2016b) Interaction between cysteine synthase and serine O-acetyltransferase proteins and their stage specific expression in Leishmania donovani. Biochimie 131:29\u201344","journal-title":"Biochimie"},{"key":"4441_CR64","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1080\/14787210.2024.2438627","volume":"23","author":"S Sundar","year":"2025","unstructured":"Sundar S, Madhukar P, Kumar R (2025) Anti-leishmanial therapies: overcoming current challenges with emerging therapies. Expert Rev Anti Infect Ther 23:159\u2013180","journal-title":"Expert Rev Anti Infect Ther"},{"issue":"3","key":"4441_CR65","doi-asserted-by":"publisher","first-page":"308","DOI":"10.2174\/2589977515666230725105141","volume":"16","author":"R Swami","year":"2024","unstructured":"Swami R, Aggarwal K (2024) The prospects of phytomedicines and nanomedicines to treat Leishmaniasis: A comprehensive review. Curr Drug Res Rev 16(3):308\u2013318. https:\/\/doi.org\/10.2174\/2589977515666230725105141","journal-title":"Curr Drug Res Rev"},{"key":"4441_CR66","doi-asserted-by":"publisher","first-page":"108087","DOI":"10.1016\/j.bioorg.2024.108087","volume":"154","author":"A Tabassum","year":"2025","unstructured":"Tabassum A, Kumari D, Bhore HB et al (2025) Synthesis of novel spiroisoxazolidino hybrids of alantolactone and isoalantolactone via 1,3 dipolar nitrone cycloaddition and its antimicrobial Evaluation. Bioorg Chem 154:108087. https:\/\/doi.org\/10.1016\/j.bioorg.2024.108087","journal-title":"Bioorg Chem"},{"issue":"12","key":"4441_CR67","doi-asserted-by":"publisher","first-page":"2214","DOI":"10.1039\/d0np00078g","volume":"38","author":"AG Tempone","year":"2021","unstructured":"Tempone AG, Pieper P, Borborema SET et al (2021) Marine alkaloids as bioactive agents against protozoal neglected tropical diseases and malaria. Nat Prod Rep 38(12):2214\u20132235. https:\/\/doi.org\/10.1039\/d0np00078g","journal-title":"Nat Prod Rep"},{"issue":"2","key":"4441_CR68","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1080\/07391102.2021.2007793","volume":"41","author":"H Tiwari","year":"2023","unstructured":"Tiwari H, Raina D, Gupta M et al (2023) Identification of novel MurA inhibitors using in silico approach, their validation and elucidation of mode of inhibition. J Biomol Struct Dyn 41(2):457\u2013468. https:\/\/doi.org\/10.1080\/07391102.2021.2007793","journal-title":"J Biomol Struct Dyn"},{"issue":"11","key":"4441_CR69","doi-asserted-by":"publisher","first-page":"e0006969","DOI":"10.1371\/journal.pntd.0006969","volume":"12","author":"L Turcano","year":"2018","unstructured":"Turcano L, Torrente E, Missineo A et al (2018) Identification and binding mode of a novel Leishmania Trypanothione reductase inhibitor from high throughput screening. PLoS Negl Trop Dis 12(11):e0006969","journal-title":"PLoS Negl Trop Dis"},{"issue":"24","key":"4441_CR70","doi-asserted-by":"publisher","first-page":"e202400383","DOI":"10.1002\/cmdc.202400383","volume":"19","author":"J Vold\u0159ich","year":"2024","unstructured":"Vold\u0159ich J, Matou\u0161ov\u00e1 M, \u0160m\u00eddkov\u00e1 M, Mertl\u00edkov\u00e1-Kaiserov\u00e1 H (2024) Fluorescence-based HTS assays for ion channel modulation in drug discovery pipelines. ChemMedChem 19(24):e202400383. https:\/\/doi.org\/10.1002\/cmdc.202400383","journal-title":"ChemMedChem"},{"issue":"1","key":"4441_CR71","doi-asserted-by":"publisher","first-page":"e0012735","DOI":"10.1371\/journal.pntd.0012735","volume":"19","author":"H Zhang","year":"2025","unstructured":"Zhang H, Yan R, Liu Y et al (2025) Progress in antileishmanial drugs: Mechanisms, challenges, and prospects. PLoS Negl Trop Dis 19(1):e0012735","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"4441_CR72","doi-asserted-by":"publisher","first-page":"1182","DOI":"10.1128\/aac.05412-11","volume":"56","author":"X Zhu","year":"2012","unstructured":"Zhu X, Pandharkar T, Werbovetz K (2012) Identification of new antileishmanial leads from hits obtained by high-throughput screening. Antimicrob Agents Chemother 56(3):1182\u20131189. https:\/\/doi.org\/10.1128\/aac.05412-11","journal-title":"Antimicrob Agents Chemother"},{"issue":"10","key":"4441_CR73","doi-asserted-by":"publisher","first-page":"1516","DOI":"10.1016\/j.drudis.2017.06.004","volume":"22","author":"B Zulfiqar","year":"2017","unstructured":"Zulfiqar B, Shelper TB, Avery VM (2017) Leishmaniasis drug discovery: recent progress and challenges in assay development. Drug Discov Today 22(10):1516\u20131531. https:\/\/doi.org\/10.1016\/j.drudis.2017.06.004","journal-title":"Drug Discov Today"}],"container-title":["World Journal of Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-025-04441-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11274-025-04441-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-025-04441-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T00:44:35Z","timestamp":1757205875000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11274-025-04441-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":73,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["4441"],"URL":"https:\/\/doi.org\/10.1007\/s11274-025-04441-4","relation":{},"ISSN":["0959-3993","1573-0972"],"issn-type":[{"value":"0959-3993","type":"print"},{"value":"1573-0972","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"21 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"247"}}