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Conventional methods, such as centrifugation, may result in low microbial recovery and false negatives, limiting diagnostic accuracy. An alternative, efficient, and accessible microbial concentration method is needed. This study evaluates a nanoparticle-based microbial concentration method to enhance pathogen recovery from CSF. The method was optimized for interaction time (1\u00a0min) and nanoparticle dosage (0.01\u00a0g\/mL) using standard microbial strains, including <jats:italic>Staphylococcus aureus<\/jats:italic>, <jats:italic>Enterococcus faecalis<\/jats:italic>, <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Pseudomonas aeruginosa<\/jats:italic>, and <jats:italic>Candida albicans<\/jats:italic>. Its clinical performance was assessed using 800 CSF samples, and microbial identification was confirmed via MALDI-TOF MS. The method significantly improved microbial recovery across 10<jats:sup>-2<\/jats:sup>\u201310<jats:sup>-9<\/jats:sup> CFU\/mL concentrations, achieving a detection limit as low as 2\u00a0CFU\/mL. Clinical validation demonstrated 100% sensitivity and specificity, detecting 15 additional true-positive cases missed by centrifugation. While centrifugation fails to detect bacteria below 10<jats:sup>-7<\/jats:sup> CFU\/mL, our method reliably detects even at 10<jats:sup>-9<\/jats:sup> CFU\/mL, demonstrating superior microbial enrichment, especially in low-biomass samples. This method enhances diagnostic accuracy by reducing false negatives and expediting pathogen detection. Its resource-conscious, low-cost and equipment-free nature makes it particularly beneficial for resource-limited laboratories, offering a scalable alternative for microbial concentration in CSF diagnostics.<\/jats:p>\n          <jats:p>\n            <jats:bold>Graphical Abstract<\/jats:bold>\n          <\/jats:p>","DOI":"10.1007\/s11274-025-04463-y","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T06:53:26Z","timestamp":1751352806000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Magnetic nanoparticle-based method for microorganism concentration in sterile body fluids: Validation and clinical applications"],"prefix":"10.1007","volume":"41","author":[{"given":"Bilsen","family":"Tural","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erdal","family":"Erta\u015f","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurullah","family":"Uzuner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bu\u015fra","family":"Bekta\u015f","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emre","family":"Tural","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet","family":"\u00c7avdar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hakan","family":"Temiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erdal","family":"\u00d6zbek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Servet","family":"Tural","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"key":"4463_CR1","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/s40824-021-00212-y","volume":"25","author":"L Abarca-Cabrera","year":"2021","unstructured":"Abarca-Cabrera L, Fraga-Garc\u00eda P, Berensmeier S (2021) Bio-nano interactions: binding proteins, polysaccharides, lipids and nucleic acids onto magnetic nanoparticles. 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This study complies with the ethical guidelines set forth by the Noninvasive Research Ethics Committee of Dicle University).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Human ethics and consent to participate"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"There are no conflicts of interest for the authors participating in the study.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Not applicable.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Clinical trial number"}}],"article-number":"240"}}