{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T18:22:30Z","timestamp":1775672550559,"version":"3.50.1"},"reference-count":67,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-21-CE43-0012-01"],"award-info":[{"award-number":["ANR-21-CE43-0012-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-21-CE43-0012-01"],"award-info":[{"award-number":["ANR-21-CE43-0012-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-21-CE43-0012-01"],"award-info":[{"award-number":["ANR-21-CE43-0012-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["World J Microbiol Biotechnol"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11274-025-04723-x","type":"journal-article","created":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T05:13:40Z","timestamp":1764998020000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Analyzing the effect of glucose availability on the ethyl acetate production by Kluyveromyces marxianus under iron limitation conditions by metabolic flux analysis approach"],"prefix":"10.1007","volume":"41","author":[{"given":"Elise","family":"Viau","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia","family":"Huesca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9830-5093","authenticated-orcid":false,"given":"Carine","family":"Bideaux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8262-6896","authenticated-orcid":false,"given":"Sandrine","family":"Alfenore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,6]]},"reference":[{"key":"4723_CR1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-023-03659-4","author":"PJ Adame-Soto","year":"2023","unstructured":"Adame-Soto PJ, Ar\u00e9chiga-Carvajal ET, Gonz\u00e1lez-Herrera SM et al (2023) Characterization of mating type on aroma production and metabolic properties wild Kluyveromyces marxianus\u00a0yeasts. World J Microbiol Biotechnol. https:\/\/doi.org\/10.1007\/s11274-023-03659-4","journal-title":"World J Microbiol Biotechnol"},{"key":"4723_CR2","doi-asserted-by":"publisher","first-page":"105511","DOI":"10.1016\/j.engfailanal.2021.105511","volume":"127","author":"AY Adesina","year":"2021","unstructured":"Adesina AY, Obot IB, Sorour AA et al (2021) Corrosion challenges and prevention in Ethyl acetate (EA) production and related processes \u2013 An overview. Eng Fail Anal 127:105511. https:\/\/doi.org\/10.1016\/j.engfailanal.2021.105511","journal-title":"Eng Fail Anal"},{"key":"4723_CR3","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1007\/BF00134726","volume":"6","author":"DW Armstrong","year":"1984","unstructured":"Armstrong DW, Yamazaki H (1984) Effect of iron and EDTA on ethyl acetate accumulation in Candida utilis. Biotechnol Lett 6:819\u2013824. https:\/\/doi.org\/10.1007\/BF00134726","journal-title":"Biotechnol Lett"},{"key":"4723_CR4","doi-asserted-by":"publisher","first-page":"1038","DOI":"10.1002\/bit.260260905","volume":"26","author":"DW Armstrong","year":"1984","unstructured":"Armstrong DW, Martin SM, Yamazaki H (1984) Production of ethyl acetate from dilute ethanol solutions by Candida utilis. Biotechnol Bioeng 26:1038\u20131041. https:\/\/doi.org\/10.1002\/bit.260260905","journal-title":"Biotechnol Bioeng"},{"key":"4723_CR5","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2022.108027","volume":"60","author":"M Baptista","year":"2022","unstructured":"Baptista M, Domingues L (2022) Kluyveromyces marxianus as a microbial cell factory for lignocellulosic biomass valorisation. Biotechnol Adv 60:108027. https:\/\/doi.org\/10.1016\/j.biotechadv.2022.108027","journal-title":"Biotechnol Adv"},{"key":"4723_CR6","doi-asserted-by":"publisher","first-page":"2134","DOI":"10.1128\/AEM.72.3.2134-2140.2006","volume":"72","author":"C Bideaux","year":"2006","unstructured":"Bideaux C, Alfenore S, Cameleyre X et al (2006) Minimization of glycerol production during the high-performance fed-batch ethanolic fermentation process in Saccharomyces cerevisiae, using a metabolic model as a prediction tool. Appl Environ Microbiol 72:2134\u20132140. https:\/\/doi.org\/10.1128\/AEM.72.3.2134-2140.2006","journal-title":"Appl Environ Microbiol"},{"key":"4723_CR7","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1504\/IJMIC.2008.020550","volume":"3","author":"C Bideaux","year":"2008","unstructured":"Bideaux C, Goma G, Uribelarrea JL et al (2008) Stoichiometric modelling approach for microbial cultures monitoring. IJMIC 3:413. https:\/\/doi.org\/10.1504\/IJMIC.2008.020550","journal-title":"IJMIC"},{"key":"4723_CR8","doi-asserted-by":"publisher","first-page":"1489","DOI":"10.1007\/s00253-015-7085-0","volume":"100","author":"C Bideaux","year":"2016","unstructured":"Bideaux C, Montheard J, Cameleyre X et al (2016) Metabolic flux analysis model for optimizing xylose conversion into ethanol by the natural C5-fermenting yeast Candida Shehatae. Appl Microbiol Biotechnol 100:1489\u20131499. https:\/\/doi.org\/10.1007\/s00253-015-7085-0","journal-title":"Appl Microbiol Biotechnol"},{"key":"4723_CR9","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1186\/s13068-020-01703-1","volume":"13","author":"AC Bohnenkamp","year":"2020","unstructured":"Bohnenkamp AC, Kruis AJ, Mars AE et al (2020) Multilevel optimisation of anaerobic ethyl acetate production in engineered Escherichia coli. Biotechnol Biofuels 13:65. https:\/\/doi.org\/10.1186\/s13068-020-01703-1","journal-title":"Biotechnol Biofuels"},{"key":"4723_CR10","doi-asserted-by":"publisher","first-page":"103176","DOI":"10.1016\/j.fbio.2023.103176","volume":"56","author":"Y Chen","year":"2023","unstructured":"Chen Y, Wan Y, Cai W et al (2023) Transcriptomic and metabonomic to evaluate the effect mechanisms of the growth and aroma-producing of Pichia anomala under ethanol stress. Food Biosci 56:103176. https:\/\/doi.org\/10.1016\/j.fbio.2023.103176","journal-title":"Food Biosci"},{"issue":"6","key":"4723_CR11","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1007\/s10295-019-02142-0","volume":"46","author":"J Dong","year":"2019","unstructured":"Dong J, Wang P, Fu X et al (2019) Increase ethyl acetate production in Saccharomyces cerevisiae by genetic engineering of ethyl acetate metabolic pathway. J Ind Microbiol Biotechnol 46(6):801\u2013808. https:\/\/doi.org\/10.1007\/s10295-019-02142-0","journal-title":"J Ind Microbiol Biotechnol"},{"key":"4723_CR12","doi-asserted-by":"publisher","first-page":"5905","DOI":"10.1128\/AEM.70.10.5905-5911.2004","volume":"70","author":"E Fredlund","year":"2004","unstructured":"Fredlund E, Blank LM, Schn\u00fcrer J et al (2004) Oxygen- and glucose-dependent regulation of central carbon metabolism in Pichia anomala. Appl Environ Microbiol 70:5905\u20135911. https:\/\/doi.org\/10.1128\/AEM.70.10.5905-5911.2004","journal-title":"Appl Environ Microbiol"},{"key":"4723_CR13","doi-asserted-by":"publisher","unstructured":"Gethins L, Guneser O, Demirkol A et al (2015) Influence of carbon and nitrogen source on production of volatile fragrance and flavour metabolites by the yeast Kluyveromyces marxianus: nutrient effects on volatiles in K. marxianus. Yeast n\/a-n\/a.\u00a0https:\/\/doi.org\/10.1002\/yea.3047\u00a0","DOI":"10.1002\/yea.3047"},{"key":"4723_CR14","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1016\/j.bjm.2016.07.018","volume":"47","author":"O G\u00fcne\u015fer","year":"2016","unstructured":"G\u00fcne\u015fer O, Karag\u00fcl-Y\u00fcceer Y, Wilkowska A, Kregiel D (2016) Volatile metabolites produced from agro-industrial wastes by Na-alginate entrapped Kluyveromyces marxianus. Braz J Microbiol 47:965\u2013972. https:\/\/doi.org\/10.1016\/j.bjm.2016.07.018","journal-title":"Braz J Microbiol"},{"key":"4723_CR15","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.3172","author":"ET Hillman","year":"2021","unstructured":"Hillman ET, Li M, Hooker CA et al (2021) Hydrolysis of lignocellulose by anaerobic fungi produces free sugars and organic acids for two-stage fine chemical production with <Emphasis Type=\"Italic\">Kluyveromyces marxianus<\/Emphasis>. Biotechnol Prog. https:\/\/doi.org\/10.1002\/btpr.3172","journal-title":"Biotechnol Prog"},{"key":"4723_CR16","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1002\/elsc.202000048","volume":"21","author":"A Hoffmann","year":"2021","unstructured":"Hoffmann A, Kupsch C, Walther T, L\u00f6ser C (2021) Synthesis of Ethyl acetate from glucose by Kluyveromyces marxianus, Cyberlindnera jadinii and Wickerhamomyces anomalus depending on the induction mode. Eng Life Sci 21:154\u2013168. https:\/\/doi.org\/10.1002\/elsc.202000048","journal-title":"Eng Life Sci"},{"key":"4723_CR17","doi-asserted-by":"publisher","first-page":"1635","DOI":"10.1007\/s00253-023-12419-1","volume":"107","author":"A Hoffmann","year":"2023","unstructured":"Hoffmann A, Franz A, Walther T, L\u00f6ser C (2023) Utilization of delactosed whey permeate for the synthesis of Ethyl acetate with Kluyveromyces Marxianus. Appl Microbiol Biotechnol 107:1635\u20131648. https:\/\/doi.org\/10.1007\/s00253-023-12419-1","journal-title":"Appl Microbiol Biotechnol"},{"issue":"12","key":"4723_CR18","doi-asserted-by":"publisher","DOI":"10.1002\/elsc.202400041","volume":"24","author":"A Hoffmann","year":"2024","unstructured":"Hoffmann A, Franz A, L\u00f6ser C et al (2024) In situ product recovery of microbially synthesized Ethyl acetate from the exhaust gas of a bioreactor by membrane technology. Eng Life Sci e202400041. https:\/\/doi.org\/10.1002\/elsc.202400041","journal-title":"Eng Life Sci"},{"key":"4723_CR19","doi-asserted-by":"publisher","unstructured":"\u0130\u015fleten Ho\u015fo\u011flu M, (2018) Study of increasing the production of volatile flavor compounds by the yeast Kluyveromyces Marxianus\u00a0through optimization of carbon and nitrogen sources. Food Health 112\u2013123. https:\/\/doi.org\/10.3153\/FH18011","DOI":"10.3153\/FH18011"},{"key":"4723_CR20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.104761","volume":"299","author":"TM Iverson","year":"2023","unstructured":"Iverson TM, Singh PK, Cecchini G (2023) An evolving view of complex II\u2014noncanonical complexes, megacomplexes, respiration, signaling, and beyond. J Biol Chem 299:104761. https:\/\/doi.org\/10.1016\/j.jbc.2023.104761","journal-title":"J Biol Chem"},{"key":"4723_CR21","unstructured":"Jaiswal C (2025) Ethyl Acetate Market Size, Share, Growth | Report, 2030. https:\/\/www.marketresearchfuture.com\/reports\/ethyl-acetate-market-945. Accessed 26 Aug 2025"},{"key":"4723_CR22","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1111\/j.1574-6968.1993.tb06387.x","volume":"111","author":"H Kallel-Mhiri","year":"1993","unstructured":"Kallel-Mhiri H, Miclo A (1993) Mechanism of ethyl acetate synthesis by Kluyveromyces fragilis. FEMS Microbiol Lett 111:207\u2013212. https:\/\/doi.org\/10.1111\/j.1574-6968.1993.tb06387.x","journal-title":"FEMS Microbiol Lett"},{"key":"4723_CR23","doi-asserted-by":"crossref","unstructured":"Kallel-Mhiri H, Engasser J-M, Miclo A (1993) Continuous ethyl acetate production by Kluyveromyces fragilis on whey permeate","DOI":"10.1007\/BF00170366"},{"key":"4723_CR24","doi-asserted-by":"publisher","first-page":"110728","DOI":"10.1016\/j.lwt.2020.110728","volume":"138","author":"H K\u0131lmano\u011flu","year":"2021","unstructured":"K\u0131lmano\u011flu H, Ho\u015fo\u011flu M\u0130, G\u00fcne\u015fer O, Y\u00fcceer YK (2021) Optimization of pretreatment and enzymatic hydrolysis conditions of tomato pomace for production of alcohols and esters by Kluyveromyces Marxianus. LWT 138:110728\u2013110728. https:\/\/doi.org\/10.1016\/j.lwt.2020.110728","journal-title":"LWT"},{"key":"4723_CR25","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.ymben.2017.03.004","volume":"41","author":"AJ Kruis","year":"2017","unstructured":"Kruis AJ, Levisson M, Mars AE et al (2017) Ethyl acetate production by the elusive alcohol acetyltransferase from yeast. Metab Eng 41:92\u2013101. https:\/\/doi.org\/10.1016\/j.ymben.2017.03.004","journal-title":"Metab Eng"},{"key":"4723_CR26","doi-asserted-by":"publisher","first-page":"e01640","DOI":"10.1128\/AEM.01640-18","volume":"84","author":"AJ Kruis","year":"2018","unstructured":"Kruis AJ, Mars AE, Kengen SWM et al (2018) Alcohol acetyltransferase Eat1 is located in yeast mitochondria. Appl Environ Microbiol 84:e01640\u2013e01618. https:\/\/doi.org\/10.1128\/AEM.01640-18","journal-title":"Appl Environ Microbiol"},{"key":"4723_CR27","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2019.06.006","volume":"37","author":"AJ Kruis","year":"2019","unstructured":"Kruis AJ, Bohnenkamp AC, Patinios C et al (2019) Microbial production of short and medium chain esters: enzymes, pathways, and applications. Biotechnol Adv 37:107407. https:\/\/doi.org\/10.1016\/j.biotechadv.2019.06.006","journal-title":"Biotechnol Adv"},{"key":"4723_CR28","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1080\/07388551.2021.1917505","volume":"41","author":"LV Leonel","year":"2021","unstructured":"Leonel LV, Arruda PV, Chandel AK et al (2021) Kluyveromyces marxianus: a potential biocatalyst of renewable chemicals and lignocellulosic ethanol production. Crit Rev Biotechnol 41:1131\u20131152. https:\/\/doi.org\/10.1080\/07388551.2021.1917505","journal-title":"Crit Rev Biotechnol"},{"key":"4723_CR29","doi-asserted-by":"publisher","first-page":"2720","DOI":"10.1271\/bbb.90609","volume":"73","author":"N Lertwattanasakul","year":"2009","unstructured":"Lertwattanasakul N, Shigemoto E, Rodrussamee N et al (2009) The crucial role of alcohol dehydrogenase Adh3 in Kluyveromyces marxianus mitochondrial metabolism. Biosci Biotechnol Biochem 73:2720\u20132726. https:\/\/doi.org\/10.1271\/bbb.90609","journal-title":"Biosci Biotechnol Biochem"},{"key":"4723_CR30","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1186\/s13068-023-02343-x","volume":"16","author":"G Liu","year":"2023","unstructured":"Liu G, Huang L, Lian J (2023) Alcohol acyltransferases for the biosynthesis of esters. Biotechnol Biofuels 16:93. https:\/\/doi.org\/10.1186\/s13068-023-02343-x","journal-title":"Biotechnol Biofuels"},{"key":"4723_CR31","doi-asserted-by":"publisher","first-page":"1274","DOI":"10.1002\/biot.201600060","volume":"11","author":"A L\u00f6bs","year":"2016","unstructured":"L\u00f6bs A, Lin J, Cook M, Wheeldon I (2016) High throughput, colorimetric screening of microbial ester biosynthesis reveals high ethyl acetate production from Kluyveromyces Marxianus on C5, C6, and C12 carbon sources. Biotechnol J 11:1274\u20131281. https:\/\/doi.org\/10.1002\/biot.201600060","journal-title":"Biotechnol J"},{"key":"4723_CR32","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1186\/s13068-017-0854-5","volume":"10","author":"A-K L\u00f6bs","year":"2017","unstructured":"L\u00f6bs A-K, Engel R, Schwartz C et al (2017) CRISPR\u2013Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces Marxianus. Biotechnol Biofuels 10:164. https:\/\/doi.org\/10.1186\/s13068-017-0854-5","journal-title":"Biotechnol Biofuels"},{"key":"4723_CR33","doi-asserted-by":"publisher","first-page":"2647","DOI":"10.1021\/acssynbio.8b00331","volume":"7","author":"A-K L\u00f6bs","year":"2018","unstructured":"L\u00f6bs A-K, Schwartz C, Thorwall S, Wheeldon I (2018) Highly multiplexed CRISPRi repression of respiratory functions enhances mitochondrial localized ethyl acetate biosynthesis in Kluyveromyces marxianus. ACS Synth Biol 7:2647\u20132655. https:\/\/doi.org\/10.1021\/acssynbio.8b00331","journal-title":"ACS Synth Biol"},{"key":"4723_CR34","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1002\/elsc.201000178","volume":"11","author":"C L\u00f6ser","year":"2011","unstructured":"L\u00f6ser C, Urit T, Nehl F, Bley T (2011) Screening of Kluyveromyces strains for the production of ethyl acetate: design and evaluation of a cultivation system. Eng Life Sci 11:369\u2013381. https:\/\/doi.org\/10.1002\/elsc.201000178","journal-title":"Eng Life Sci"},{"key":"4723_CR35","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1007\/s00253-012-4205-y","volume":"96","author":"C L\u00f6ser","year":"2012","unstructured":"L\u00f6ser C, Urit T, F\u00f6rster S et al (2012) Formation of ethyl acetate by Kluyveromyces Marxianus on whey during aerobic batch and chemostat cultivation at iron limitation. Appl Microbiol Biotechnol 96:685\u2013696. https:\/\/doi.org\/10.1007\/s00253-012-4205-y","journal-title":"Appl Microbiol Biotechnol"},{"key":"4723_CR36","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.jbiotec.2012.10.009","volume":"163","author":"C L\u00f6ser","year":"2013","unstructured":"L\u00f6ser C, Urit T, Stukert A, Bley T (2013) Formation of ethyl acetate from whey by Kluyveromyces marxianus on a pilot scale. J Biotechnol 163:17\u201323. https:\/\/doi.org\/10.1016\/j.jbiotec.2012.10.009","journal-title":"J Biotechnol"},{"key":"4723_CR37","doi-asserted-by":"publisher","first-page":"5397","DOI":"10.1007\/s00253-014-5765-9","volume":"98","author":"C L\u00f6ser","year":"2014","unstructured":"L\u00f6ser C, Urit T, Bley T (2014) Perspectives for the biotechnological production of ethyl acetate by yeasts. Appl Microbiol Biotechnol 98:5397\u20135415. https:\/\/doi.org\/10.1007\/s00253-014-5765-9","journal-title":"Appl Microbiol Biotechnol"},{"key":"4723_CR38","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/s13705-014-0028-2","volume":"5","author":"C L\u00f6ser","year":"2015","unstructured":"L\u00f6ser C, Urit T, Gruner E, Bley T (2015a) Efficient growth of Kluyveromyces Marxianus biomass used as a biocatalyst in the sustainable production of ethyl acetate. Energ Sustain Soc 5:2. https:\/\/doi.org\/10.1186\/s13705-014-0028-2","journal-title":"Energ Sustain Soc"},{"key":"4723_CR39","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1007\/s00253-014-6098-4","volume":"99","author":"C L\u00f6ser","year":"2015","unstructured":"L\u00f6ser C, Urit T, Keil P, Bley T (2015b) Studies on the mechanism of synthesis of ethyl acetate in Kluyveromyces Marxianus DSM 5422. Appl Microbiol Biotechnol 99:1131\u20131144. https:\/\/doi.org\/10.1007\/s00253-014-6098-4","journal-title":"Appl Microbiol Biotechnol"},{"key":"4723_CR40","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1002\/elsc.201700195","volume":"18","author":"C L\u00f6ser","year":"2018","unstructured":"L\u00f6ser C, Haas C, Liu W et al (2018) Uptake of iron by Kluyveromyces marxianus DSM 5422 cultivated in a whey-based medium. Eng Life Sci 18:459\u2013474. https:\/\/doi.org\/10.1002\/elsc.201700195","journal-title":"Eng Life Sci"},{"key":"4723_CR41","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1002\/elsc.202000047","volume":"21","author":"C L\u00f6ser","year":"2021","unstructured":"L\u00f6ser C, Kupsch C, Walther T, Hoffmann A (2021) A new approach for balancing the microbial synthesis of ethyl acetate and other volatile metabolites during aerobic bioreactor cultivations. Eng Life Sci 21:137\u2013153. https:\/\/doi.org\/10.1002\/elsc.202000047","journal-title":"Eng Life Sci"},{"key":"4723_CR42","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1016\/j.renene.2022.12.100","volume":"204","author":"A Maghzian","year":"2023","unstructured":"Maghzian A, Aslani A, Zahedi R, Yaghoubi M (2023) How to effectively produce value-added products from microalgae? Renew Energy 204:262\u2013276. https:\/\/doi.org\/10.1016\/j.renene.2022.12.100","journal-title":"Renew Energy"},{"key":"4723_CR43","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1007\/s11274-017-2215-8","volume":"33","author":"MT Mart\u00ednez-Pastor","year":"2017","unstructured":"Mart\u00ednez-Pastor MT, Perea-Garc\u00eda A, Puig S (2017) Mechanisms of iron sensing and regulation in the yeast Saccharomyces cerevisiae. World J Microbiol Biotechnol 33:75. https:\/\/doi.org\/10.1007\/s11274-017-2215-8","journal-title":"World J Microbiol Biotechnol"},{"key":"4723_CR44","doi-asserted-by":"publisher","first-page":"4585","DOI":"10.1007\/s00253-016-7392-0","volume":"100","author":"J Martynova","year":"2016","unstructured":"Martynova J, Kokina A, Kibilds J et al (2016) Effects of acetate on Kluyveromyces Marxianus DSM 5422 growth and metabolism. Appl Microbiol Biotechnol 100:4585\u20134594. https:\/\/doi.org\/10.1007\/s00253-016-7392-0","journal-title":"Appl Microbiol Biotechnol"},{"key":"4723_CR45","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/S1369-703X(00)00065-6","volume":"6","author":"ABP Medeiros","year":"2000","unstructured":"Medeiros ABP, Pandey A, Freitas RJS et al (2000) Optimization of the production of aroma compounds by Kluyveromyces marxianus in solid-state fermentation using factorial design and response surface methodology. Biochem Eng J 6:33\u201339. https:\/\/doi.org\/10.1016\/S1369-703X(00)00065-6","journal-title":"Biochem Eng J"},{"key":"4723_CR46","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1002\/bit.10122","volume":"77","author":"K M\u00f8ller","year":"2002","unstructured":"M\u00f8ller K, Christensen B, F\u00f6rster J et al (2002) Aerobic glucose metabolism of Saccharomyces kluyveri: growth, metabolite production, and quantification of metabolic fluxes. Biotech & Bioengineering 77:186\u2013193. https:\/\/doi.org\/10.1002\/bit.10122","journal-title":"Biotech & Bioengineering"},{"key":"4723_CR47","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/yea.3054","volume":"32","author":"JP Morrissey","year":"2015","unstructured":"Morrissey JP, Etschmann MMW, Schrader J, de Billerbeck GM (2015) Cell factory applications of the yeast Kluyveromyces marxianus for the biotechnological production of natural flavour and fragrance molecules: Natural flavour and fragrance molecules from K. marxianus. Yeast 32:3\u201316. https:\/\/doi.org\/10.1002\/yea.3054","journal-title":"Yeast"},{"key":"4723_CR48","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/s11274-023-03811-0","volume":"40","author":"LA Mu\u00f1oz-Miranda","year":"2024","unstructured":"Mu\u00f1oz-Miranda LA, Zepeda-Pe\u00f1a AC, Casas-Godoy L et al (2024) CRISPRi-induced transcriptional regulation of IAH1 gene and its influence on volatile compounds profile in Kluyveromyces Marxianus DU3. World J Microbiol Biotechnol 40:121. https:\/\/doi.org\/10.1007\/s11274-023-03811-0","journal-title":"World J Microbiol Biotechnol"},{"key":"4723_CR49","doi-asserted-by":"publisher","first-page":"5711","DOI":"10.1002\/anie.201200625","volume":"51","author":"M Nielsen","year":"2012","unstructured":"Nielsen M, Junge H, Kammer A, Beller M (2012) Towards a green process for bulk-scale synthesis of ethyl acetate: efficient acceptorless dehydrogenation of ethanol. Angew Chem Int Ed 51:5711\u20135713. https:\/\/doi.org\/10.1002\/anie.201200625","journal-title":"Angew Chem Int Ed"},{"key":"4723_CR50","unstructured":"Pattanaik BN, Mandalia HC (2011) Ethyl acetate: properties, production processes and applications - a review. Int J Curr Res Rev 3(12). https:\/\/www.semanticscholar.org\/paper\/ETHYL-ACETATE-%3A-PROPERTIES-%2C-PRODUCTION-PROCESSES-Pattanaik-Mandalia\/27e8c9e8030d3de73ed4b3cd6f1cfd1460bf833a"},{"key":"4723_CR51","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1007\/s10295-017-1946-8","volume":"44","author":"A Pentjuss","year":"2017","unstructured":"Pentjuss A, Stalidzans E, Liepins J et al (2017) Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism. J Ind Microbiol Biotechnol 44:1177\u20131190. https:\/\/doi.org\/10.1007\/s10295-017-1946-8","journal-title":"J Ind Microbiol Biotechnol"},{"key":"4723_CR52","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2023.108125","volume":"64","author":"Y Qiu","year":"2023","unstructured":"Qiu Y, Lei P, Wang R et al (2023) Kluyveromyces as promising yeast cell factories for industrial bioproduction: From bio-functional design to applications. Biotechnol Adv 64:108125. https:\/\/doi.org\/10.1016\/j.biotechadv.2023.108125","journal-title":"Biotechnol Adv"},{"key":"4723_CR53","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/S0168-1605(01)00552-9","volume":"70","author":"V Rojas","year":"2001","unstructured":"Rojas V, Gil JV, Pi\u00f1aga F, Manzanares P (2001) Studies on acetate ester production by non-Saccharomyces wine yeasts. Int J Food Microbiol 70:283\u2013289. https:\/\/doi.org\/10.1016\/S0168-1605(01)00552-9","journal-title":"Int J Food Microbiol"},{"key":"4723_CR54","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1021\/acssynbio.0c00446","volume":"10","author":"W Shi","year":"2021","unstructured":"Shi W, Li J, Chen Y et al (2021) Metabolic engineering of Saccharomyces cerevisiae for ethyl acetate biosynthesis. ACS Synth Biol 10:495\u2013504. https:\/\/doi.org\/10.1021\/acssynbio.0c00446","journal-title":"ACS Synth Biol"},{"key":"4723_CR55","doi-asserted-by":"publisher","first-page":"1846","DOI":"10.1016\/j.biotechadv.2013.08.006","volume":"31","author":"P-Y Stergiou","year":"2013","unstructured":"Stergiou P-Y, Foukis A, Filippou M et al (2013) Advances in lipase-catalyzed esterification reactions. Biotechnol Adv 31:1846\u20131859. https:\/\/doi.org\/10.1016\/j.biotechadv.2013.08.006","journal-title":"Biotechnol Adv"},{"key":"4723_CR56","doi-asserted-by":"publisher","first-page":"1665","DOI":"10.1007\/s12010-013-0273-4","volume":"170","author":"Z Tavsan","year":"2013","unstructured":"Tavsan Z, Ayar Kayali H (2013) The effect of iron and copper as an essential nutrient on mitochondrial electron transport system and lipid peroxidation in Trichoderma Harzianum. Appl Biochem Biotechnol 170:1665\u20131675. https:\/\/doi.org\/10.1007\/s12010-013-0273-4","journal-title":"Appl Biochem Biotechnol"},{"key":"4723_CR57","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1139\/m78-073","volume":"24","author":"KC Thomas","year":"1978","unstructured":"Thomas C, Dawson S (1978) Relationship between iron-limited growth and energy limitation during phased cultivation of Candida utilis. Can J Microbiol 24:440\u2013447. https:\/\/doi.org\/10.1139\/m78-073","journal-title":"Can J Microbiol"},{"key":"4723_CR58","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1007\/s00449-010-0504-9","volume":"34","author":"T Urit","year":"2011","unstructured":"Urit T, L\u00f6ser C, Wunderlich M, Bley T (2011) Formation of ethyl acetate by Kluyveromyces Marxianus on whey: studies of the ester stripping. Bioprocess Biosyst Eng 34:547\u2013559. https:\/\/doi.org\/10.1007\/s00449-010-0504-9","journal-title":"Bioprocess Biosyst Eng"},{"key":"4723_CR59","doi-asserted-by":"publisher","first-page":"1313","DOI":"10.1007\/s00253-012-4107-z","volume":"96","author":"T Urit","year":"2012","unstructured":"Urit T, Stukert A, Bley T, L\u00f6ser C (2012) Formation of ethyl acetate by Kluyveromyces Marxianus on whey during aerobic batch cultivation at specific trace element limitation. Appl Microbiol Biotechnol 96:1313\u20131323. https:\/\/doi.org\/10.1007\/s00253-012-4107-z","journal-title":"Appl Microbiol Biotechnol"},{"key":"4723_CR60","doi-asserted-by":"publisher","first-page":"10359","DOI":"10.1007\/s00253-013-5278-y","volume":"97","author":"T Urit","year":"2013","unstructured":"Urit T, Li M, Bley T, Loser C (2013a) Growth of Kluyveromyces marxianus and formation of ethyl acetate depending on temperature. Appl Microbiol Biotechnol 97:10359\u201310371. https:\/\/doi.org\/10.1007\/s00253-013-5278-y","journal-title":"Appl Microbiol Biotechnol"},{"key":"4723_CR61","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1002\/elsc.201200077","volume":"13","author":"T Urit","year":"2013","unstructured":"Urit T, Manthey R, Bley T, L\u00f6ser C (2013b) Formation of ethyl acetate by Kluyveromyces marxianus on whey: influence of aeration and inhibition of yeast growth by ethyl acetate. Eng Life Sci 13:247\u2013260. https:\/\/doi.org\/10.1002\/elsc.201200077","journal-title":"Eng Life Sci"},{"key":"4723_CR62","doi-asserted-by":"publisher","unstructured":"Wilkowska A, Kregiel D, Guneser O, Karagul Yuceer Y (2014) Growth and by-product profiles of Kluyveromyces Marxianus cells immobilized in foamed alginate: By-product profiles of K. Marxianus immobilized in foamed alginate.\u00a0Yeast n\/a-\/a.\u00a0https:\/\/doi.org\/10.1002\/yea.3044.","DOI":"10.1002\/yea.3044"},{"key":"4723_CR63","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1007\/BF00399980","volume":"56","author":"A Willetts","year":"1989","unstructured":"Willetts A (1989) Ester formation from ethanol by Candida Pseudotropicalis. Antonie Van Leeuwenhoek 56:175\u2013180. https:\/\/doi.org\/10.1007\/BF00399980","journal-title":"Antonie Van Leeuwenhoek"},{"key":"4723_CR64","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1002\/j.2050-0416.2008.tb00303.x","volume":"114","author":"M Yilmaztekin","year":"2008","unstructured":"Yilmaztekin M, Erten H, Cabaroglu T (2008) Production of isoamyl acetate from sugar beet molasses by williopsis Saturnus var. Saturnus. J Inst Brew 114:34\u201338. https:\/\/doi.org\/10.1002\/j.2050-0416.2008.tb00303.x","journal-title":"J Inst Brew"},{"key":"4723_CR65","doi-asserted-by":"publisher","first-page":"7239","DOI":"10.1007\/s00253-020-10756-z","volume":"104","author":"S Zhang","year":"2020","unstructured":"Zhang S, Guo F, Yan W et al (2020a) Perspectives for the microbial production of ethyl acetate. Appl Microbiol Biotechnol 104:7239\u20137245. https:\/\/doi.org\/10.1007\/s00253-020-10756-z","journal-title":"Appl Microbiol Biotechnol"},{"key":"4723_CR66","doi-asserted-by":"publisher","first-page":"109641","DOI":"10.1016\/j.enzmictec.2020.109641","volume":"140","author":"B Zhang","year":"2020","unstructured":"Zhang B, Ren L, Wang H et al (2020b) Glycerol uptake and synthesis systems contribute to the osmotic tolerance of Kluyveromyces marxianus. Enzyme Microb Technol 140:109641\u2013109641. https:\/\/doi.org\/10.1016\/j.enzmictec.2020.109641","journal-title":"Enzyme Microb Technol"},{"key":"4723_CR67","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2020.107820","volume":"165","author":"B Zhang","year":"2021","unstructured":"Zhang B, Ren L, Zhao Z et al (2021) High temperature xylitol production through simultaneous co-utilization of glucose and xylose by engineered Kluyveromyces marxianus. Biochem Eng J 165:107820. https:\/\/doi.org\/10.1016\/j.bej.2020.107820","journal-title":"Biochem Eng J"}],"container-title":["World Journal of Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-025-04723-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11274-025-04723-x","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-025-04723-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T17:02:39Z","timestamp":1767718959000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11274-025-04723-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":67,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["4723"],"URL":"https:\/\/doi.org\/10.1007\/s11274-025-04723-x","relation":{},"ISSN":["0959-3993","1573-0972"],"issn-type":[{"value":"0959-3993","type":"print"},{"value":"1573-0972","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"24 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"All tables and Figs.\u00a02, 3, 4 and 5 were made using MSOffice. For Figs.\u00a02, 3, 4 and 5; Tables\u00a04 and 6, GIMP software was used to ensure its quality to be 600 dpi. Each single graph of Fig.\u00a01 was made using R Studio, and both MSOffice and GIMP were used to assemble and ensure its quality to be 1200 dpi.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Tables and figures"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"499"}}