{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:18:32Z","timestamp":1787591912919,"version":"build-2736575974"},"reference-count":81,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["No. 2024YFA0917900"],"award-info":[{"award-number":["No. 2024YFA0917900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Science and Technology Innovation Action Plan","award":["grant no. 23HC1400500"],"award-info":[{"award-number":["grant no. 23HC1400500"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["World J Microbiol Biotechnol"],"DOI":"10.1007\/s11274-026-04993-z","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T08:17:30Z","timestamp":1778228250000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["rDNA-mediated multicopy integration and gene dosage quantification system for microbial zeaxanthin biosynthesis"],"prefix":"10.1007","volume":"42","author":[{"given":"Ruijing","family":"Ling","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuting","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangzhao","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Secundo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiyi","family":"Xi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"issue":"4","key":"4993_CR1","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1046\/j.1365-313x.1995.7040649.x","volume":"7","author":"H Albert","year":"1995","unstructured":"Albert H, Dale EC, Lee E, Ow DW (1995) Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J 7(4):649\u2013659. https:\/\/doi.org\/10.1046\/j.1365-313x.1995.7040649.x","journal-title":"Plant J"},{"issue":"4","key":"4993_CR2","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1007\/s00253-011-3488-8","volume":"92","author":"JM Araya-Garay","year":"2011","unstructured":"Araya-Garay JM, Feijoo-Siota L, Veiga-Crespo P, Villa TG (2011) cDNA cloning of a novel gene codifying for the enzyme lycopene \u03b2-cyclase from Ficus carica and its expression in Escherichia coli. Appl Microbiol Biotechnol 92(4):769\u2013777. https:\/\/doi.org\/10.1007\/s00253-011-3488-8","journal-title":"Appl Microbiol Biotechnol"},{"key":"4993_CR3","doi-asserted-by":"publisher","first-page":"353","DOI":"10.2147\/btt.S326422","volume":"15","author":"M Asmamaw","year":"2021","unstructured":"Asmamaw M, Zawdie B (2021) Mechanism and Applications of CRISPR\/Cas-9-Mediated Genome Editing. Biologics 15:353\u2013361. https:\/\/doi.org\/10.2147\/btt.S326422","journal-title":"Biologics"},{"issue":"1","key":"4993_CR4","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1186\/1475-2859-12-128","volume":"12","author":"R Aw","year":"2013","unstructured":"Aw R, Polizzi KM (2013) Can too many copies spoil the broth? Microb Cell Fact 12(1):128. https:\/\/doi.org\/10.1186\/1475-2859-12-128","journal-title":"Microb Cell Fact"},{"issue":"8","key":"4993_CR5","doi-asserted-by":"publisher","first-page":"1978","DOI":"10.1021\/acssynbio.0c00048","volume":"9","author":"M Babaei","year":"2020","unstructured":"Babaei M, Borja Zamfir GM, Chen X, Christensen HB, Kristensen M, Nielsen J, Borodina I (2020) Metabolic Engineering of Saccharomyces cerevisiae for Rosmarinic Acid Production. ACS Synth Biol 9(8):1978\u20131988. https:\/\/doi.org\/10.1021\/acssynbio.0c00048","journal-title":"ACS Synth Biol"},{"issue":"7","key":"4993_CR6","doi-asserted-by":"publisher","first-page":"4953","DOI":"10.1128\/MCB.19.7.4953","volume":"19","author":"M Banditt","year":"1999","unstructured":"Banditt M, Koller T, Sogo JM (1999) Transcriptional activity and chromatin structure of enhancer-deleted rRNA genes in Saccharomyces cerevisiae. Mol Cell Biol 19(7):4953\u20134960. https:\/\/doi.org\/10.1128\/MCB.19.7.4953","journal-title":"Mol Cell Biol"},{"issue":"1","key":"4993_CR7","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1186\/s12934-017-0715-8","volume":"16","author":"MO Betancur","year":"2017","unstructured":"Betancur MO, Reis VCB, Nicola AM, De Marco JL, de Moraes LMP, Torres FAG (2017) Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker. Microb Cell Fact 16(1):99. https:\/\/doi.org\/10.1186\/s12934-017-0715-8","journal-title":"Microb Cell Fact"},{"issue":"3","key":"4993_CR8","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/0092-8674(85)90197-7","volume":"40","author":"JD Boeke","year":"1985","unstructured":"Boeke JD, Garfinkel DJ, Styles CA, Fink GR (1985) Ty elements transpose through an RNA intermediate. Cell 40(3):491\u2013500. https:\/\/doi.org\/10.1016\/0092-8674(85)90197-7","journal-title":"Cell"},{"issue":"13","key":"4993_CR9","doi-asserted-by":"publisher","first-page":"7791","DOI":"10.1093\/nar\/gkab535","volume":"49","author":"P Cai","year":"2021","unstructured":"Cai P, Duan X, Wu X, Gao L, Ye M, Zhou YJ (2021) Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris. Nucleic Acids Res 49(13):7791\u20137805. https:\/\/doi.org\/10.1093\/nar\/gkab535","journal-title":"Nucleic Acids Res"},{"issue":"23","key":"4993_CR10","doi-asserted-by":"publisher","first-page":"7335","DOI":"10.1128\/aem.01241-09","volume":"75","author":"J Caspi","year":"2009","unstructured":"Caspi J, Barak Y, Haimovitz R, Irwin D, Lamed R, Wilson DB, Bayer EA (2009) Effect of linker length and dockerin position on conversion of a Thermobifida fusca endoglucanase to the cellulosomal mode. Appl Environ Microbiol 75(23):7335\u20137342. https:\/\/doi.org\/10.1128\/aem.01241-09","journal-title":"Appl Environ Microbiol"},{"issue":"6334","key":"4993_CR11","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1126\/science.aak9787","volume":"356","author":"C Chen","year":"2017","unstructured":"Chen C, Xing D, Tan L, Li H, Zhou G, Huang L, Xie XS (2017) Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion. Science 356(6334):189\u2013194. https:\/\/doi.org\/10.1126\/science.aak9787","journal-title":"Science"},{"issue":"2","key":"4993_CR12","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/j.synbio.2025.02.016","volume":"10","author":"X Chen","year":"2025","unstructured":"Chen X, Li C, Qiu X, Chen M, Xu Y, Li S, Li Q, Wang L (2025) CRISPR\/Cas9-based iterative multi-copy integration for improved metabolite yields in Saccharomyces cerevisiae. Synth Syst Biotechnol 10(2):629\u2013637. https:\/\/doi.org\/10.1016\/j.synbio.2025.02.016","journal-title":"Synth Syst Biotechnol"},{"issue":"6121","key":"4993_CR13","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1126\/science.1231143","volume":"339","author":"L Cong","year":"2013","unstructured":"Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex genome engineering using CRISPR\/Cas systems. Science 339(6121):819\u2013823. https:\/\/doi.org\/10.1126\/science.1231143","journal-title":"Science"},{"key":"4993_CR14","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/j.ymben.2013.10.004","volume":"20","author":"Z Dai","year":"2013","unstructured":"Dai Z, Liu Y, Zhang X, Shi M, Wang B, Wang D, Huang L, Zhang X (2013) Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides. Metab Eng 20:146\u2013156. https:\/\/doi.org\/10.1016\/j.ymben.2013.10.004","journal-title":"Metab Eng"},{"issue":"6","key":"4993_CR15","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1038\/nbt.1544","volume":"27","author":"K De Schutter","year":"2009","unstructured":"De Schutter K, Lin Y-C, Tiels P, Van Hecke A, Glinka S, Weber-Lehmann J, Rouz\u00e9 P, Van de Peer Y, Callewaert N (2009) Genome sequence of the recombinant protein production host Pichia pastoris. Nat Biotechnol 27(6):561\u2013566. https:\/\/doi.org\/10.1038\/nbt.1544","journal-title":"Nat Biotechnol"},{"issue":"1","key":"4993_CR16","doi-asserted-by":"publisher","first-page":"1178","DOI":"10.1038\/s41467-020-14981-y","volume":"11","author":"OX Dong","year":"2020","unstructured":"Dong OX, Yu S, Jain R, Zhang N, Duong PQ, Butler C, Li Y, Lipzen A, Martin JA, Barry KW, Schmutz J, Tian L, Ronald PC (2020) Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9. Nat Commun 11(1):1178. https:\/\/doi.org\/10.1038\/s41467-020-14981-y","journal-title":"Nat Commun"},{"issue":"1","key":"4993_CR17","doi-asserted-by":"publisher","first-page":"21106","DOI":"10.1038\/s41598-020-77876-4","volume":"10","author":"M Fourni\u00e9","year":"2020","unstructured":"Fourni\u00e9 M, Truan G (2020) Multiplicity of carotene patterns derives from competition between phytoene desaturase diversification and biological environments. Sci Rep 10(1):21106. https:\/\/doi.org\/10.1038\/s41598-020-77876-4","journal-title":"Sci Rep"},{"issue":"2","key":"4993_CR18","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1021\/acssynbio.1c00307","volume":"11","author":"J Gao","year":"2022","unstructured":"Gao J, Xu J, Zuo Y, Ye C, Jiang L, Feng L, Huang L, Xu Z, Lian J (2022) Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR\/Cas9. ACS Synth Biol 11(2):623\u2013633. https:\/\/doi.org\/10.1021\/acssynbio.1c00307","journal-title":"ACS Synth Biol"},{"issue":"1","key":"4993_CR19","doi-asserted-by":"publisher","first-page":"11730","DOI":"10.1038\/srep11730","volume":"5","author":"Y Guo","year":"2015","unstructured":"Guo Y, Wu J, Jia H, Chen W, Shao C, Zhao L, Ma J, Li R, Zhong Y, Fang F, Wang D, Sun J, Qian F, Dai X, Zhang G, Tian Z, Li X, Xiao B, W (2015) Balancing the Expression and Production of a Heterodimeric Protein: Recombinant Agkisacutacin as a Novel Antithrombotic Drug Candidate. Sci Rep 5(1):11730. https:\/\/doi.org\/10.1038\/srep11730","journal-title":"Sci Rep"},{"issue":"6","key":"4993_CR20","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1038\/s41580-022-00573-9","volume":"24","author":"Y Hori","year":"2023","unstructured":"Hori Y, Engel C, Kobayashi T (2023) Regulation of ribosomal RNA gene copy number, transcription and nucleolus organization in eukaryotes. Nat Rev Mol Cell Biol 24(6):414\u2013429. https:\/\/doi.org\/10.1038\/s41580-022-00573-9","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"2","key":"4993_CR21","doi-asserted-by":"publisher","first-page":"fuad008","DOI":"10.1093\/femsre\/fuad008","volume":"47","author":"Y Hoshino","year":"2023","unstructured":"Hoshino Y, Villanueva L (2023) Four billion years of microbial terpenome evolution. FEMS Microbiol Rev 47(2):fuad008. https:\/\/doi.org\/10.1093\/femsre\/fuad008","journal-title":"FEMS Microbiol Rev"},{"issue":"1","key":"4993_CR22","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1186\/s13068-023-02377-1","volume":"16","author":"D Huang","year":"2023","unstructured":"Huang D, Liu C, Su M, Zeng Z, Wang C, Hu Z, Lou S, Li H (2023) Enhancement of \u03b2-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene \u03b2-cyclase. Biotechnol Biofuels Bioprod 16(1):127. https:\/\/doi.org\/10.1186\/s13068-023-02377-1","journal-title":"Biotechnol Biofuels Bioprod"},{"key":"4993_CR23","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1016\/j.procbio.2023.11.033","volume":"136","author":"F Islam","year":"2024","unstructured":"Islam F, Khan J, Zehravi M, Das R, Haque MA, Banu A, Parwaiz S, Nainu F, Nafady MH, Shahriar SMS, Hossain MJ, Muzammil K, Emran TB (2024) Synergistic effects of carotenoids: Therapeutic benefits on human health. Process Biochem 136:254\u2013272. https:\/\/doi.org\/10.1016\/j.procbio.2023.11.033","journal-title":"Process Biochem"},{"key":"4993_CR24","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.ymben.2015.01.008","volume":"28","author":"T Jako\u010di\u016bnas","year":"2015","unstructured":"Jako\u010di\u016bnas T, Bonde I, Herrg\u00e5rd M, Harrison SJ, Kristensen M, Pedersen LE, Jensen MK, Keasling JD (2015) Multiplex metabolic pathway engineering using CRISPR\/Cas9 in Saccharomyces cerevisiae. Metab Eng 28:213\u2013222. https:\/\/doi.org\/10.1016\/j.ymben.2015.01.008","journal-title":"Metab Eng"},{"issue":"2","key":"4993_CR25","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1111\/1567-1364.12118","volume":"14","author":"NB Jensen","year":"2014","unstructured":"Jensen NB, Strucko T, Kildegaard KR, David F, Maury J, Mortensen UH, Forster J, Nielsen J, Borodina I (2014) EasyClone: method for iterative chromosomal integration of multiple genes Saccharomyces cerevisiae. FEMS Yeast Res 14(2):238\u2013248. https:\/\/doi.org\/10.1111\/1567-1364.12118","journal-title":"FEMS Yeast Res"},{"issue":"8","key":"4993_CR26","doi-asserted-by":"publisher","first-page":"1110","DOI":"10.1002\/biot.201600147","volume":"11","author":"MM Jessop-Fabre","year":"2016","unstructured":"Jessop-Fabre MM, Jako\u010di\u016bnas T, Stovicek V, Dai Z, Jensen MK, Keasling JD, Borodina I (2016) EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J 11(8):1110\u20131117. https:\/\/doi.org\/10.1002\/biot.201600147","journal-title":"Biotechnol J"},{"issue":"11","key":"4993_CR27","doi-asserted-by":"publisher","first-page":"940","DOI":"10.1021\/cbe.4c00104","volume":"1","author":"X Jiang","year":"2024","unstructured":"Jiang X, Li M, Wang Z, Ye C, Gao J, Ai X, Bao J, Cheng J, Lian J (2024) CRISPR-Mediated rDNA Integration and Fluorescence Screening for Pathway Optimization in Pichia pastoris. Chem Bio Eng 1(11):940\u2013951. https:\/\/doi.org\/10.1021\/cbe.4c00104","journal-title":"Chem Bio Eng"},{"issue":"1","key":"4993_CR28","doi-asserted-by":"publisher","first-page":"4248","DOI":"10.1038\/s41467-019-12247-w","volume":"10","author":"W Kang","year":"2019","unstructured":"Kang W, Ma T, Liu M, Qu J, Liu Z, Zhang H, Shi B, Fu S, Ma J, Lai LTF, He S, Qu J, Wing-Ngor Au S, Kang H, Lau BY, Deng WC, Xia Z, Liu J, T (2019) Modular enzyme assembly for enhanced cascade biocatalysis and metabolic flux. Nat Commun 10(1):4248. https:\/\/doi.org\/10.1038\/s41467-019-12247-w","journal-title":"Nat Commun"},{"key":"4993_CR29","doi-asserted-by":"publisher","first-page":"827704","DOI":"10.3389\/ffunb.2022.827704","volume":"3","author":"LB Kastberg","year":"2022","unstructured":"Kastberg LB, Ard R, Jensen MK, Workman CT (2022) Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies. Front Fungal Biol 3:827704. https:\/\/doi.org\/10.3389\/ffunb.2022.827704","journal-title":"Front Fungal Biol"},{"issue":"8","key":"4993_CR30","doi-asserted-by":"publisher","first-page":"1395","DOI":"10.1007\/s00018-010-0613-2","volume":"68","author":"T Kobayashi","year":"2011","unstructured":"Kobayashi T (2011) Regulation of ribosomal RNA gene copy number and its role in modulating genome integrity and evolutionary adaptability in yeast. Cell Mol Life Sci 68(8):1395\u20131403. https:\/\/doi.org\/10.1007\/s00018-010-0613-2","journal-title":"Cell Mol Life Sci"},{"issue":"5740","key":"4993_CR31","doi-asserted-by":"publisher","first-page":"1581","DOI":"10.1126\/science.1116102","volume":"309","author":"T Kobayashi","year":"2005","unstructured":"Kobayashi T, Ganley AR (2005) Recombination regulation by transcription-induced cohesin dissociation in rDNA repeats. Science 309(5740):1581\u20131584. https:\/\/doi.org\/10.1126\/science.1116102","journal-title":"Science"},{"issue":"4","key":"4993_CR32","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1016\/S0092-8674(04)00414-3","volume":"117","author":"T Kobayashi","year":"2004","unstructured":"Kobayashi T, Horiuchi T, Tongaonkar P, Vu L, Nomura M (2004) Regulates Recombination between Different rDNA Repeats, but Not Recombination within Individual rRNA Genes in Yeast. Cell 117(4):441\u2013453. https:\/\/doi.org\/10.1016\/S0092-8674(04)00414-3","journal-title":"Cell"},{"issue":"3","key":"4993_CR33","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1007\/s002530051059","volume":"48","author":"FW Lee","year":"1997","unstructured":"Lee FW, Silva D, N. A (1997) Improved efficiency and stability of multiple cloned gene insertions at the delta sequences of Saccharomyces cerevisiae. Appl Microbiol Biotechnol 48(3):339\u2013345. https:\/\/doi.org\/10.1007\/s002530051059","journal-title":"Appl Microbiol Biotechnol"},{"key":"4993_CR34","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.jbiotec.2020.11.009","volume":"325","author":"H Li","year":"2021","unstructured":"Li H, Gao S, Zhang S, Zeng W, Zhou J (2021) Effects of metabolic pathway gene copy numbers on the biosynthesis of (2S)-naringenin in Saccharomyces cerevisiae. J Biotechnol 325:119\u2013127. https:\/\/doi.org\/10.1016\/j.jbiotec.2020.11.009","journal-title":"J Biotechnol"},{"issue":"6","key":"4993_CR35","doi-asserted-by":"publisher","first-page":"e00159","DOI":"10.1128\/aem.00159-25","volume":"91","author":"X Li","year":"2025","unstructured":"Li X, Liu Y, Ma L, Jiang W, Shi T, Li L, Li C, Chen Z, Fan X, Xu Q (2025) Metabolic engineering of Escherichia coli for high-yield dopamine production via optimized fermentation strategies. Appl Environ Microbiol 91(6):e00159\u2013e00125. https:\/\/doi.org\/10.1128\/aem.00159-25","journal-title":"Appl Environ Microbiol"},{"key":"4993_CR36","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.ymben.2018.04.011","volume":"50","author":"J Lian","year":"2018","unstructured":"Lian J, Mishra S, Zhao H (2018) Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications. Metab Eng 50:85\u2013108. https:\/\/doi.org\/10.1016\/j.ymben.2018.04.011","journal-title":"Metab Eng"},{"issue":"1\u20132","key":"4993_CR37","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/s0378-1119(00)00576-x","volume":"263","author":"GP Lin Cereghino","year":"2001","unstructured":"Lin Cereghino GP, Lin Cereghino J, Sunga AJ, Johnson MA, Lim M, Gleeson MA, Cregg JM (2001) New selectable marker\/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris. Gene 263(1\u20132):159\u2013169. https:\/\/doi.org\/10.1016\/s0378-1119(00)00576-x","journal-title":"Gene"},{"issue":"15","key":"4993_CR39","doi-asserted-by":"publisher","first-page":"4943","DOI":"10.1128\/jb.174.15.4943-4951.1992","volume":"174","author":"H Liu","year":"1992","unstructured":"Liu H, Tan X, Veenhuis M, McCollum D, Cregg JM (1992) An efficient screen for peroxisome-deficient mutants of Pichia pastoris. J Bacteriol 174(15):4943\u20134951. https:\/\/doi.org\/10.1128\/jb.174.15.4943-4951.1992","journal-title":"J Bacteriol"},{"issue":"1","key":"4993_CR38","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1007\/s11705-016-1601-3","volume":"11","author":"D Liu","year":"2017","unstructured":"Liu D, Li B, Liu H, Guo X, Yuan Y (2017) Profiling influences of gene overexpression on heterologous resveratrol production in Saccharomyces cerevisiae. Front Chem Sci Eng 11(1):117\u2013125. https:\/\/doi.org\/10.1007\/s11705-016-1601-3","journal-title":"Front Chem Sci Eng"},{"issue":"1","key":"4993_CR40","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1186\/s12934-019-1194-x","volume":"18","author":"Q Liu","year":"2019","unstructured":"Liu Q, Shi X, Song L, Liu H, Zhou X, Wang Q, Zhang Y, Cai M (2019) CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris. Microb Cell Fact 18(1):144. https:\/\/doi.org\/10.1186\/s12934-019-1194-x","journal-title":"Microb Cell Fact"},{"issue":"1","key":"4993_CR41","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1038\/s41467-022-28277-w","volume":"13","author":"Y Ma","year":"2022","unstructured":"Ma Y, Liu N, Greisen P, Li J, Qiao K, Huang S, Stephanopoulos G (2022) Removal of lycopene substrate inhibition enables high carotenoid productivity in Yarrowia lipolytica. Nat Commun 13(1):572. https:\/\/doi.org\/10.1038\/s41467-022-28277-w","journal-title":"Nat Commun"},{"issue":"28","key":"4993_CR42","doi-asserted-by":"publisher","first-page":"11784","DOI":"10.1073\/pnas.0702984104","volume":"104","author":"JA Maresca","year":"2007","unstructured":"Maresca JA, Graham JE, Wu M, Eisen JA, Bryant DA (2007) Identification of a fourth family of lycopene cyclases in photosynthetic bacteria. Proc Natl Acad Sci 104(28):11784\u201311789. https:\/\/doi.org\/10.1073\/pnas.0702984104","journal-title":"Proc Natl Acad Sci"},{"issue":"3","key":"4993_CR43","doi-asserted-by":"publisher","first-page":"e0150394","DOI":"10.1371\/journal.pone.0150394","volume":"11","author":"J Maury","year":"2016","unstructured":"Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, Herrg\u00e5rd MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I (2016) EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae. PLoS ONE 11(3):e0150394. https:\/\/doi.org\/10.1371\/journal.pone.0150394","journal-title":"PLoS ONE"},{"issue":"20","key":"4993_CR44","doi-asserted-by":"publisher","first-page":"12785","DOI":"10.1021\/acs.chemrev.6b00077","volume":"116","author":"G Meinke","year":"2016","unstructured":"Meinke G, Bohm A, Hauber J, Pisabarro MT, Buchholz F (2016) Cre Recombinase and Other Tyrosine Recombinases. Chem Rev 116(20):12785\u201312820. https:\/\/doi.org\/10.1021\/acs.chemrev.6b00077","journal-title":"Chem Rev"},{"key":"4993_CR45","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.jbiotec.2016.07.006","volume":"233","author":"HY Moon","year":"2016","unstructured":"Moon HY, Lee DW, Sim GH, Kim H-J, Hwang JY, Kwon M-G, Kang B-K, Kim JM, Kang HA (2016) A new set of rDNA-NTS-based multiple integrative cassettes for the development of antibiotic-marker-free recombinant yeasts. J Biotechnol 233:190\u2013199. https:\/\/doi.org\/10.1016\/j.jbiotec.2016.07.006","journal-title":"J Biotechnol"},{"issue":"22","key":"4993_CR46","doi-asserted-by":"publisher","first-page":"e140","DOI":"10.1093\/nar\/gng140","volume":"31","author":"P Oberdoerffer","year":"2003","unstructured":"Oberdoerffer P, Otipoby KL, Maruyama M, Rajewsky K (2003) Unidirectional Cre-mediated genetic inversion in mice using the mutant loxP pair lox66\/lox71. Nucleic Acids Res 31(22):e140. https:\/\/doi.org\/10.1093\/nar\/gng140","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"4993_CR47","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1021\/bp9500627","volume":"12","author":"RN Parekh","year":"1996","unstructured":"Parekh RN, Shaw MR, Wittrup KD (1996) An integrating vector for tunable, high copy, stable integration into the dispersed Ty delta sites of Saccharomyces cerevisiae. Biotechnol Prog 12(1):16\u201321. https:\/\/doi.org\/10.1021\/bp9500627","journal-title":"Biotechnol Prog"},{"issue":"1","key":"4993_CR48","doi-asserted-by":"publisher","first-page":"2895","DOI":"10.1038\/s41467-022-30529-8","volume":"13","author":"B Peng","year":"2022","unstructured":"Peng B, Esquirol L, Lu Z, Shen Q, Cheah LC, Howard CB, Scott C, Trau M, Dumsday G, Vickers CE (2022) An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae. Nat Commun 13(1):2895. https:\/\/doi.org\/10.1038\/s41467-022-30529-8","journal-title":"Nat Commun"},{"key":"4993_CR49","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1146\/annurev-chembioeng-061010-114209","volume":"2","author":"LB Pickens","year":"2011","unstructured":"Pickens LB, Tang Y, Chooi YH (2011) Metabolic engineering for the production of natural products. Annu Rev Chem Biomol Eng 2:211\u2013236. https:\/\/doi.org\/10.1146\/annurev-chembioeng-061010-114209","journal-title":"Annu Rev Chem Biomol Eng"},{"key":"4993_CR50","doi-asserted-by":"publisher","first-page":"2022","DOI":"10.3389\/fmicb.2022.851706","volume":"13","author":"H Qi","year":"2022","unstructured":"Qi H, Yu L, Li Y, Cai M, He J, Liu J, Hao L, Xu H, Qiao M (2022) Developing Multi-Copy Chromosomal Integration Strategies for Heterologous Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae. Front Microbiol 13:2022. https:\/\/doi.org\/10.3389\/fmicb.2022.851706","journal-title":"Front Microbiol"},{"key":"4993_CR51","doi-asserted-by":"publisher","first-page":"e00086","DOI":"10.1016\/j.mec.2019.e00086","volume":"8","author":"H Rabeharindranto","year":"2019","unstructured":"Rabeharindranto H, Casta\u00f1o-Cerezo S, Lautier T, Garcia-Alles LF, Treitz C, Tholey A, Truan G (2019) Enzyme-fusion strategies for redirecting and improving carotenoid synthesis in S. cerevisiae. Metabolic Eng Commun 8:e00086. https:\/\/doi.org\/10.1016\/j.mec.2019.e00086","journal-title":"Metabolic Eng Commun"},{"issue":"6","key":"4993_CR52","first-page":"1240","volume":"10","author":"H Ren","year":"2022","unstructured":"Ren H, Yin A, Wu P, Zhou H, Zhou J, Yu Y, Lu H (2022) Establishment of a Cre-loxP System Based on a Leaky LAC4 Promoter and an Unstable panARS Element in Kluyveromyces marxianus. Microbe 10(6):1240. https:\/\/www.mdpi.com\/2076-2607\/10\/6\/1240","journal-title":"Microbe"},{"issue":"1","key":"4993_CR53","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1111\/1751-7915.12187","volume":"8","author":"K Roos","year":"2015","unstructured":"Roos K, Werner E, Loessner H (2015) Multicopy integration of mini-Tn7 transposons into selected chromosomal sites of a Salmonella vaccine strain. Microb Biotechnol 8(1):177\u2013187. https:\/\/doi.org\/10.1111\/1751-7915.12187","journal-title":"Microb Biotechnol"},{"key":"4993_CR54","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2014.00172","volume":"5","author":"GL Rosano","year":"2014","unstructured":"Rosano GL, Ceccarelli EA (2014) Recombinant protein expression in Escherichia coli: advances and challenges. Front Microbiol 5:1\u201317. https:\/\/doi.org\/10.3389\/fmicb.2014.00172","journal-title":"Front Microbiol"},{"issue":"1","key":"4993_CR55","doi-asserted-by":"publisher","first-page":"3908","DOI":"10.1038\/s41467-022-31422-0","volume":"13","author":"MV Rouches","year":"2022","unstructured":"Rouches MV, Xu Y, Cortes LBG, Lambert G (2022) A plasmid system with tunable copy number. Nat Commun 13(1):3908. https:\/\/doi.org\/10.1038\/s41467-022-31422-0","journal-title":"Nat Commun"},{"issue":"4","key":"4993_CR56","doi-asserted-by":"publisher","first-page":"795","DOI":"10.3390\/antiox11040795","volume":"11","author":"RK Saini","year":"2022","unstructured":"Saini RK, Prasad P, Lokesh V, Shang X, Shin J, Keum YS, Lee JH (2022) Carotenoids: Dietary Sources, Extraction, Encapsulation, Bioavailability, and Health Benefits-A Review of Recent Advancements. Antioxidants 11(4):795 Article 795. https:\/\/doi.org\/10.3390\/antiox11040795","journal-title":"Antioxidants"},{"issue":"3","key":"4993_CR57","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1007\/bf00164526","volume":"33","author":"A Sakai","year":"1990","unstructured":"Sakai A, Shimizu Y, Hishinuma F (1990) Integration of heterologous genes into the chromosome of Saccharomyces cerevisiae using a delta sequence of yeast retrotransposon Ty. Appl Microbiol Biotechnol 33(3):302\u2013306. https:\/\/doi.org\/10.1007\/bf00164526","journal-title":"Appl Microbiol Biotechnol"},{"issue":"7","key":"4993_CR58","doi-asserted-by":"publisher","first-page":"4185","DOI":"10.1073\/pnas.022039799","volume":"99","author":"SW Santoro","year":"2002","unstructured":"Santoro SW, Schultz PG (2002) Directed evolution of the site specificity of Cre recombinase. Proc Natl Acad Sci 99(7):4185\u20134190. https:\/\/doi.org\/10.1073\/pnas.022039799","journal-title":"Proc Natl Acad Sci"},{"key":"4993_CR59","doi-asserted-by":"publisher","unstructured":"Schmidt SR (2024) Fusion Proteins: Current Status and Future Perspectives. In K. Gadamasetti & S. A. Kolodziej, Bioprocessing, bioengineering and process chemistry in the biopharmaceutical industry: using chemistry and bioengineering to improve the performance of biologics, 287\u2013343. https:\/\/doi.org\/10.1007\/978-3-031-62007-2_11","DOI":"10.1007\/978-3-031-62007-2_11"},{"issue":"1","key":"4993_CR60","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1186\/s12934-022-01781-w","volume":"21","author":"KN Schwaiger","year":"2022","unstructured":"Schwaiger KN, Voit A, Wiltschi B, Nidetzky B (2022) Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production. Microb Cell Fact 21(1):61. https:\/\/doi.org\/10.1186\/s12934-022-01781-w","journal-title":"Microb Cell Fact"},{"issue":"3","key":"4993_CR61","doi-asserted-by":"publisher","first-page":"918","DOI":"10.1104\/pp.18.01280","volume":"179","author":"N Smirnoff","year":"2019","unstructured":"Smirnoff N (2019) Engineering of Metabolic Pathways Using Synthetic Enzyme Complexes. Plant Physiol 179(3):918\u2013928. https:\/\/doi.org\/10.1104\/pp.18.01280","journal-title":"Plant Physiol"},{"issue":"9","key":"4993_CR62","doi-asserted-by":"publisher","first-page":"5202","DOI":"10.1039\/d3gc04949c","volume":"26","author":"M-L Sun","year":"2024","unstructured":"Sun M-L, Han Y, Yu X, Wang K, Lin L, Ledesma-Amaro R, Ji X-J (2024) Constructing a green oleaginous yeast cell factory for sustainable production of the plant-derived diterpenoid sclareol. Green Chem 26(9):5202\u20135210. https:\/\/doi.org\/10.1039\/d3gc04949c","journal-title":"Green Chem"},{"issue":"1","key":"4993_CR63","doi-asserted-by":"publisher","first-page":"2136","DOI":"10.1038\/s41467-018-04543-8","volume":"9","author":"LJ Sweetlove","year":"2018","unstructured":"Sweetlove LJ, Fernie AR (2018) The role of dynamic enzyme assemblies and substrate channelling in metabolic regulation. Nat Commun 9(1):2136. https:\/\/doi.org\/10.1038\/s41467-018-04543-8","journal-title":"Nat Commun"},{"key":"4993_CR64","doi-asserted-by":"publisher","first-page":"1708","DOI":"10.1038\/s41587-024-02462-2","volume":"43","author":"MJ Szarzanowicz","year":"2025","unstructured":"Szarzanowicz MJ, Waldburger LM, Busche M, Geiselman GM, Kirkpatrick LD, Kehl AJ, Tahmin C, Kuo RC, McCauley J, Pannu H, Cui R, Liu S, Hillson NJ, Brunkard JO, Keasling JD, Gladden JM, Thompson MG, Shih PM (2025) Binary vector copy number engineering improves Agrobacterium-mediated transformation. Nat Biotechnol 43:1708\u20131716. https:\/\/doi.org\/10.1038\/s41587-024-02462-2","journal-title":"Nat Biotechnol"},{"issue":"17","key":"4993_CR65","doi-asserted-by":"publisher","first-page":"7799","DOI":"10.1039\/d3fo02330c","volume":"14","author":"J Terao","year":"2023","unstructured":"Terao J (2023) Revisiting carotenoids as dietary antioxidants for human health and disease prevention. Food Function 14(17):7799\u20137824. https:\/\/doi.org\/10.1039\/d3fo02330c","journal-title":"Food Function"},{"issue":"5","key":"4993_CR68","doi-asserted-by":"publisher","first-page":"826","DOI":"10.1021\/acssynbio.6b00323","volume":"6","author":"Y Wang","year":"2017","unstructured":"Wang Y, Heermann R, Jung K (2017) CipA and CipB as Scaffolds To Organize Proteins into Crystalline Inclusions. ACS Synth Biol 6(5):826\u2013836. https:\/\/doi.org\/10.1021\/acssynbio.6b00323","journal-title":"ACS Synth Biol"},{"issue":"1","key":"4993_CR66","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1186\/s12934-019-1105-1","volume":"18","author":"C Wang","year":"2019","unstructured":"Wang C, Zhao S, Shao X, Park JB, Jeong SH, Park HJ, Kwak WJ, Wei G, Kim SW (2019) Challenges and tackles in metabolic engineering for microbial production of carotenoids. Microb Cell Fact 18(1):55. https:\/\/doi.org\/10.1186\/s12934-019-1105-1","journal-title":"Microb Cell Fact"},{"issue":"2","key":"4993_CR67","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1007\/s10529-025-03566-8","volume":"47","author":"F Wang","year":"2025","unstructured":"Wang F, Singh S, Permaul K (2025) Improving the hydrophilic microenvironment surrounding the catalytic site of fructosyltransferase enhances its catalytic ability. Biotechnol Lett 47(2):30. https:\/\/doi.org\/10.1007\/s10529-025-03566-8","journal-title":"Biotechnol Lett"},{"key":"4993_CR69","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.jbiotec.2016.03.027","volume":"235","author":"A Weninger","year":"2016","unstructured":"Weninger A, Hatzl AM, Schmid C, Vogl T, Glieder A (2016) Combinatorial optimization of CRISPR\/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris. J Biotechnol 235:139\u2013149. https:\/\/doi.org\/10.1016\/j.jbiotec.2016.03.027","journal-title":"J Biotechnol"},{"key":"4993_CR70","doi-asserted-by":"publisher","first-page":"699235","DOI":"10.3389\/fmicb.2021.699235","volume":"12","author":"Y Xie","year":"2021","unstructured":"Xie Y, Chen S, Xiong X (2021) Metabolic Engineering of Non-carotenoid-Producing Yeast Yarrowia lipolytica for the Biosynthesis of Zeaxanthin. Front Microbiol 12:699235. https:\/\/doi.org\/10.3389\/fmicb.2021.699235","journal-title":"Front Microbiol"},{"key":"4993_CR72","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1016\/j.biortech.2018.08.044","volume":"268","author":"R Yamada","year":"2018","unstructured":"Yamada R, Yamauchi A, Ando Y, Kumata Y, Ogino H (2018) Modulation of gene expression by cocktail \u03b4-integration to improve carotenoid production in Saccharomyces cerevisiae. Bioresour Technol 268:616\u2013621. https:\/\/doi.org\/10.1016\/j.biortech.2018.08.044","journal-title":"Bioresour Technol"},{"issue":"2","key":"4993_CR71","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1007\/s11274-019-2610-4","volume":"35","author":"R Yamada","year":"2019","unstructured":"Yamada R, Ogura K, Kimoto Y, Ogino H (2019) Toward the construction of a technology platform for chemicals production from methanol: d-lactic acid production from methanol by an engineered yeast Pichia pastoris. World J Microbiol Biotechnol 35(2):37., Article 37. https:\/\/doi.org\/10.1007\/s11274-019-2610-4","journal-title":"World J Microbiol Biotechnol"},{"issue":"1","key":"4993_CR73","doi-asserted-by":"publisher","first-page":"16249","DOI":"10.1038\/s41598-017-16577-x","volume":"7","author":"XW Yu","year":"2017","unstructured":"Yu XW, Sun WH, Wang YZ, Xu Y (2017) Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects. Sci Rep 7(1):16249. https:\/\/doi.org\/10.1038\/s41598-017-16577-x","journal-title":"Sci Rep"},{"issue":"1","key":"4993_CR74","doi-asserted-by":"publisher","first-page":"5836","DOI":"10.1038\/s41467-022-33606-0","volume":"13","author":"H Zhang","year":"2022","unstructured":"Zhang H, Fu X, Gong X, Wang Y, Zhang H, Zhao Y, Shen Y (2022) Systematic dissection of key factors governing recombination outcomes by GCE-SCRaMbLE. Nat Commun 13(1):5836. https:\/\/doi.org\/10.1038\/s41467-022-33606-0","journal-title":"Nat Commun"},{"key":"4993_CR75","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1016\/j.ymben.2020.10.004","volume":"63","author":"D Zhao","year":"2021","unstructured":"Zhao D, Zhu X, Zhou H, Sun N, Wang T, Bi C, Zhang X (2021a) CRISPR-based metabolic pathway engineering. Metab Eng 63:148\u2013159. https:\/\/doi.org\/10.1016\/j.ymben.2020.10.004","journal-title":"Metab Eng"},{"issue":"48","key":"4993_CR76","doi-asserted-by":"publisher","first-page":"14609","DOI":"10.1021\/acs.jafc.1c05387","volume":"69","author":"L Zhao","year":"2021","unstructured":"Zhao L, Chen Z, Lin S, Wu T, Yu S, Huo Y-X (2021b) In Vitro Biosynthesis of Isobutyraldehyde Through the Establishment of a One-Step Self-Assembly-Based Immobilization Strategy. J Agric Food Chem 69(48):14609\u201314619. https:\/\/doi.org\/10.1021\/acs.jafc.1c05387","journal-title":"J Agric Food Chem"},{"issue":"5","key":"4993_CR77","doi-asserted-by":"publisher","first-page":"1511","DOI":"10.1111\/1751-7915.14010","volume":"15","author":"H Zheng","year":"2022","unstructured":"Zheng H, Wang K, Xu X, Pan J, Sun X, Hou J, Liu W, Shen Y (2022) Highly efficient rDNA-mediated multicopy integration based on the dynamic balance of rDNA in Saccharomyces cerevisiae. Microb Biotechnol 15(5):1511\u20131524. https:\/\/doi.org\/10.1111\/1751-7915.14010","journal-title":"Microb Biotechnol"},{"issue":"1","key":"4993_CR78","doi-asserted-by":"publisher","first-page":"9790","DOI":"10.1038\/s41467-024-54191-4","volume":"15","author":"W Zheng","year":"2024","unstructured":"Zheng W, Wang Y, Cui J, Guo G, Li Y, Hou J, Tu Q, Yin Y, Stewart F, Zhang Y, Bian X, Wang X (2024) ReaL-MGE is a tool for enhanced multiplex genome engineering and application to malonyl-CoA anabolism. Nat Commun 15(1):9790. https:\/\/doi.org\/10.1038\/s41467-024-54191-4","journal-title":"Nat Commun"},{"issue":"1","key":"4993_CR79","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1007\/s00253-024-13037-1","volume":"108","author":"W Zhou","year":"2024","unstructured":"Zhou W, Li Y, Liu G, Qin W, Wei D, Wang F, Gao B (2024) CRISPR\/Cas9-based toolkit for rapid marker recycling and combinatorial libraries in Komagataella phaffii. Appl Microbiol Biotechnol 108(1):197. https:\/\/doi.org\/10.1007\/s00253-024-13037-1","journal-title":"Appl Microbiol Biotechnol"},{"key":"4993_CR80","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.ymben.2025.03.013","volume":"90","author":"W Zhou","year":"2025","unstructured":"Zhou W, Ling RJ, Yang YC, Hou ST, Wang FQ, Gao B, Wei DZ (2025) Engineering Komagataella phaffii to produce lycopene sustainably from glucose or methanol. Metab Eng 90:141\u2013153. https:\/\/doi.org\/10.1016\/j.ymben.2025.03.013","journal-title":"Metab Eng"},{"issue":"1","key":"4993_CR81","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/s12934-023-02275-z","volume":"23","author":"L Zhu","year":"2024","unstructured":"Zhu L, Song Y, Ma S, Yang S (2024) Heterologous production of 3-hydroxypropionic acid in Methylorubrum extorquens by introducing the mcr gene via a multi-round chromosomal integration system based on cre-lox71\/lox66 and transposon. Microb Cell Fact 23(1):5. https:\/\/doi.org\/10.1186\/s12934-023-02275-z","journal-title":"Microb Cell Fact"}],"container-title":["World Journal of Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-026-04993-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11274-026-04993-z","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-026-04993-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T11:17:01Z","timestamp":1781954221000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11274-026-04993-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,8]]},"references-count":81,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["4993"],"URL":"https:\/\/doi.org\/10.1007\/s11274-026-04993-z","relation":{},"ISSN":["1573-0972"],"issn-type":[{"value":"1573-0972","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,8]]},"assertion":[{"value":"18 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 April 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 May 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"265"}}