{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T11:51:17Z","timestamp":1781956277079,"version":"3.54.5"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"JST SPRING","award":["JPMJSP2139"],"award-info":[{"award-number":["JPMJSP2139"]}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP22H03803"],"award-info":[{"award-number":["JP22H03803"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["World J Microbiol Biotechnol"],"DOI":"10.1007\/s11274-026-05041-6","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T05:59:59Z","timestamp":1779861599000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Production of \u03b2-carotene from methanol via rDNA-targeted multi-gene integration and selection with increasing antibiotic concentrations in Komagataella phaffii"],"prefix":"10.1007","volume":"42","author":[{"given":"Yoshifumi","family":"Inoue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3820-7501","authenticated-orcid":false,"given":"Ryosuke","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyasu","family":"Ogino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,27]]},"reference":[{"key":"5041_CR1","doi-asserted-by":"publisher","first-page":"441","DOI":"10.3390\/biom13030441","volume":"13","author":"GD Barone","year":"2023","unstructured":"Barone GD, Emmerstorfer-Augustin A, Biundo A, Pisano I, Coccetti P, Mapelli V, Camattari A (2023) Industrial Production of Proteins with Pichia pastoris\u2014Komagataella phaffii. Biomolecules 13:441. https:\/\/doi.org\/10.3390\/biom13030441","journal-title":"Biomolecules"},{"issue":"10","key":"5041_CR2","doi-asserted-by":"publisher","first-page":"1095","DOI":"10.1016\/j.procbio.2009.05.012","volume":"44","author":"A Bhataya","year":"2009","unstructured":"Bhataya A, Schmidt-Dannert C, Lee PC (2009) Metabolic engineering of Pichia pastoris X-33 for lycopene production. Process Biochem 44(10):1095\u20131102. https:\/\/doi.org\/10.1016\/j.procbio.2009.05.012","journal-title":"Process Biochem"},{"key":"5041_CR3","doi-asserted-by":"publisher","first-page":"6923","DOI":"10.1007\/s00253-018-9154-7","volume":"102","author":"JN Br\u00f6ker","year":"2018","unstructured":"Br\u00f6ker JN, M\u00fcller B, van Deenen N, Pr\u00fcfer D, Schulze Gronover C (2018) Upregulating the mevalonate pathway and repressing sterol synthesis in Saccharomyces cerevisiae enhances the production of triterpenes. Appl Microbiol Biotechnol 102:6923\u20136934. https:\/\/doi.org\/10.1007\/s00253-018-9154-7","journal-title":"Appl Microbiol Biotechnol"},{"key":"5041_CR4","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-020-01809-6","volume":"13","author":"X Bu","year":"2020","unstructured":"Bu X, Lin JY, Cheng J, Yang D, Duan CQ, Koffas M, Yan GL (2020) Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of \u03b2-carotene in Saccharomyces cerevisiae. Biotechnol Biofuels 13:168. https:\/\/doi.org\/10.1186\/s13068-020-01809-6","journal-title":"Biotechnol Biofuels"},{"key":"5041_CR5","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-021-01723-y","volume":"21","author":"X Bu","year":"2022","unstructured":"Bu X, Lin JY, Duan CQ, Koffas MAG, Yan GL (2022) Dual regulation of lipid droplet-triacylglycerol metabolism and ERG9 expression for improved \u03b2-carotene production in Saccharomyces cerevisiae. Microb Cell Fact 21:3. https:\/\/doi.org\/10.1186\/s12934-021-01723-y","journal-title":"Microb Cell Fact"},{"key":"5041_CR6","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1002\/yea.3807","volume":"39","author":"HL Cai","year":"2022","unstructured":"Cai HL, Shimada M, Nakagawa T (2022) The potential and capability of the methylotrophic yeast Ogataea methanolica in a methanol bioeconomy. Yeast 39:440\u2013448. https:\/\/doi.org\/10.1002\/yea.3807","journal-title":"Yeast"},{"key":"5041_CR7","doi-asserted-by":"publisher","first-page":"2962","DOI":"10.1002\/bit.26846","volume":"115","author":"A Cankorur-Cetinkaya","year":"2018","unstructured":"Cankorur-Cetinkaya A, Narraidoo N, Kasavi C, Slater NKH, Archer DB, Oliver SG (2018) Process development for the continuous production of heterologous proteins by the industrial yeast Komagataella phaffii. Biotechnol Bioeng 115:2962\u20132973. https:\/\/doi.org\/10.1002\/bit.26846","journal-title":"Biotechnol Bioeng"},{"key":"5041_CR8","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.synbio.2020.06.005","volume":"5","author":"X Cao","year":"2020","unstructured":"Cao X, Yang S, Cao C, Zhou YJ (2020) Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast. Synth Syst Biotechnol 5:179\u2013186. https:\/\/doi.org\/10.1016\/j.synbio.2020.06.005","journal-title":"Synth Syst Biotechnol"},{"key":"5041_CR9","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/j.copbio.2019.06.002","volume":"59","author":"JE Fischer","year":"2019","unstructured":"Fischer JE, Glieder A (2019) Current advances in engineering tools for Pichia pastoris. Curr Opin Biotechnol 59:175\u2013181. https:\/\/doi.org\/10.1016\/j.copbio.2019.06.002","journal-title":"Curr Opin Biotechnol"},{"key":"5041_CR10","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1021\/acssynbio.1c00307","volume":"11","author":"J Gao","year":"2022","unstructured":"Gao J, Xu J, Zuo Y, Ye C, Jiang L, Feng L, Huang L, Xu Z, Lian J (2022) Synthetic biology toolkit for marker-less integration of multigene pathways into Pichia pastoris via CRISPR\/Cas9. ACS Synth Biol 11:623\u2013633. https:\/\/doi.org\/10.1021\/acssynbio.1c00307","journal-title":"ACS Synth Biol"},{"key":"5041_CR11","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1186\/s13068-024-02596-0","volume":"17","author":"Y Inoue","year":"2024","unstructured":"Inoue Y, Yamada R, Matsumoto T, Ogino H (2024) Enhancing D-lactic acid production by optimizing the expression of D-LDH gene in methylotrophic yeast Komagataella phaffii. Biotechnol Biofuels Bioprod 17:149. https:\/\/doi.org\/10.1186\/s13068-024-02596-0","journal-title":"Biotechnol Biofuels Bioprod"},{"key":"5041_CR12","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1021\/cbe.4c00104","volume":"1","author":"X Jiang","year":"2024","unstructured":"Jiang X, Li Y, Chen X, Wang Y, Zhang Y, Zhou X (2024) CRISPR-mediated rDNA integration and fluorescence screening for pathway optimization in Pichia pastoris. Chem Biol Eng 1:11\u201326. https:\/\/doi.org\/10.1021\/cbe.4c00104","journal-title":"Chem Biol Eng"},{"key":"5041_CR13","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1002\/bit.26473","volume":"115","author":"M Larroude","year":"2018","unstructured":"Larroude M, Celinska E, Back A, Thomas S, Nicaud JM, Ledesma-Amaro R (2018) A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of \u03b2-carotene. Biotechnol Bioeng 115:464\u2013472. https:\/\/doi.org\/10.1002\/bit.26473","journal-title":"Biotechnol Bioeng"},{"key":"5041_CR15","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1007\/s11274-005-9091-3","volume":"22","author":"D Libkind","year":"2006","unstructured":"Libkind D, van Broock MR (2006) Biomass and carotenoid pigment production by Patagonian native yeasts. World J Microbiol Biotechnol 22:687\u2013692. https:\/\/doi.org\/10.1007\/s11274-005-9091-3","journal-title":"World J Microbiol Biotechnol"},{"key":"5041_CR16","doi-asserted-by":"publisher","DOI":"10.1038\/srep18439","volume":"6","author":"WC Liu","year":"2016","unstructured":"Liu WC, Inwood S, Gong T, Sharma A, Yu LY, Rowe-McNally R, Roy I (2016) Scaling-up fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement. Sci Rep 6:18439. https:\/\/doi.org\/10.1038\/srep18439","journal-title":"Sci Rep"},{"key":"5041_CR17","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.ymben.2017.12.009","volume":"45","author":"Y Liu","year":"2018","unstructured":"Liu Y, Tu X, Bai C, Kong C, Liu Q, Yu J, Peng Q, Zhou X, Zhang Y, Cai M (2018) Engineered monoculture and co-culture of methylotrophic yeast for de novo production of Monacolin J and lovastatin from methanol. Metab Eng 45:189\u2013199. https:\/\/doi.org\/10.1016\/j.ymben.2017.12.009","journal-title":"Metab Eng"},{"key":"5041_CR14","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/j.procbio.2024.06.002","volume":"144","author":"Y Li","year":"2024","unstructured":"Li Y, Zhou W, Ling R, Hou S, Zhang L, Gao B (2024) Revealing the endogenous homoserine cycle for the effective methanol conversion in Pichia pastoris. Process Biochem 144:287\u2013293. https:\/\/doi.org\/10.1016\/j.procbio.2024.06.002","journal-title":"Process Biochem"},{"key":"5041_CR18","unstructured":"Markets and Markets (2020) Carotenoids Market Report, Code R (4411) FB. https:\/\/www.marketsandmarkets.com\/Market-Reports\/carotenoid-market-158421566.html. Accessed 28 Oct 2025"},{"key":"5041_CR19","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-13-12","volume":"13","author":"LC Mata-G\u00f3mez","year":"2014","unstructured":"Mata-G\u00f3mez LC, Monta\u00f1ez JC, M\u00e9ndez-Zavala A, Aguilar CN (2014) Biotechnological production of carotenoids by yeasts: an overview. Microb Cell Fact 13:12. https:\/\/doi.org\/10.1186\/1475-2859-13-12","journal-title":"Microb Cell Fact"},{"key":"5041_CR20","doi-asserted-by":"publisher","first-page":"1999","DOI":"10.1080\/10408398.2020.1867959","volume":"62","author":"AJ Mel\u00e9ndez-Mart\u00ednez","year":"2022","unstructured":"Mel\u00e9ndez-Mart\u00ednez AJ, Mandi\u0107 AI, Bantis F, B\u00f6hm V, Borge GIA, Brn\u010di\u0107 M, Bysted A, Cano MP, Dias MG, Elgersma A, Fikselov\u00e1 M, Garc\u00eda-Alonso J, Giuffrida D, Gon\u00e7alves VSS, Hornero-M\u00e9ndez D, Kljak K, Lavelli V, Manganaris GA, Mapelli-Brahm P, Marounek M, Olmedilla-Alonso B, Periago-Cast\u00f3n MJ, Pintea A, Sheehan JJ, Tumbas \u0160aponjac V, Val\u0161\u00edkov\u00e1-Frey M, Van Meulebroek L, O\u2019Brien N (2022) A comprehensive review on carotenoids in foods and feeds: status quo, applications, patents, and research needs. Crit Rev Food Sci Nutr 62:1999\u20132049. https:\/\/doi.org\/10.1080\/10408398.2020.1867959","journal-title":"Crit Rev Food Sci Nutr"},{"key":"5041_CR21","doi-asserted-by":"publisher","first-page":"2636","DOI":"10.1002\/anie.200462121","volume":"44","author":"GA Olah","year":"2005","unstructured":"Olah GA (2005) Beyond oil and gas: the methanol economy. Angew Chem Int Ed 44:2636\u20132639. https:\/\/doi.org\/10.1002\/anie.200462121","journal-title":"Angew Chem Int Ed"},{"key":"5041_CR22","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1007\/s10295-016-1808-9","volume":"43","author":"ML Olson","year":"2016","unstructured":"Olson ML, Johnson J, Carswell WF, Reyes LH, Senger RS, Kao KC (2016) Characterization of an evolved carotenoids hyper-producer of Saccharomyces cerevisiae through bioreactor parameter optimization and Raman spectroscopy. J Ind Microbiol Biotechnol 43:1355\u20131363. https:\/\/doi.org\/10.1007\/s10295-016-1808-9","journal-title":"J Ind Microbiol Biotechnol"},{"issue":"2","key":"5041_CR23","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation9020147","volume":"9","author":"D Paul","year":"2023","unstructured":"Paul D, Kumari PK, Siddiqui N (2023) Yeast carotenoids: cost-effective fermentation strategies for health care applications. Fermentation 9(2):147. https:\/\/doi.org\/10.3390\/fermentation9020147","journal-title":"Fermentation"},{"key":"5041_CR24","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.jbiotec.2017.02.027","volume":"247","author":"H Pollmann","year":"2017","unstructured":"Pollmann H, Breitenbach J, Sandmann G (2017) Development of Xanthophyllomyces dendrorhous as a production system for the colorless carotene phytoene. J Biotechnol 247:34\u201341. https:\/\/doi.org\/10.1016\/j.jbiotec.2017.02.027","journal-title":"J Biotechnol"},{"key":"5041_CR25","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1393-8","volume":"16","author":"R Prielhofer","year":"2015","unstructured":"Prielhofer R, Maurer M, Klein J, Wenger J, Kiziak C, Gasser B, Mattanovich D (2015) Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional rather than the translational level. BMC Genomics 16:167. https:\/\/doi.org\/10.1186\/s12864-015-1393-8","journal-title":"BMC Genomics"},{"key":"5041_CR26","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.ymben.2013.11.002","volume":"21","author":"LH Reyes","year":"2014","unstructured":"Reyes LH, Gomez JM, Kao KC (2014) Improving carotenoids production in yeast via adaptive laboratory evolution. Metab Eng 21:26\u201333. https:\/\/doi.org\/10.1016\/j.ymben.2013.11.002","journal-title":"Metab Eng"},{"key":"5041_CR27","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1186\/s12915-015-0186-5","volume":"13","author":"H Ru\u00dfmayer","year":"2015","unstructured":"Ru\u00dfmayer H, Buchetics M, Gruber C, Valli M, Grillitsch K, Modarres G, Guerrasio R, Klavins K, Neubauer S, Drexler H, Steiger MG, Troyer C, Al Chalabi A, Krebiehl G, Sonntag D, Zellnig G, Daum G, Graf AB, Altmann F, Koellensperger G, Hann S, Sauer M, Mattanovich D, Gasser B (2015) Systems-level organization of yeast methylotrophic lifestyle. BMC Biol 13:80. https:\/\/doi.org\/10.1186\/s12915-015-0186-5","journal-title":"BMC Biol"},{"key":"5041_CR28","doi-asserted-by":"publisher","first-page":"00285","DOI":"10.1002\/biot.202300285","volume":"19","author":"S Shimazaki","year":"2024","unstructured":"Shimazaki S, Yamada R, Yamamoto Y, Matsumoto T, Ogino H (2024) Building a machine-learning model to predict optimal mevalonate pathway gene expression levels for efficient production of a carotenoid in yeast. Biotechnol J 19:00285. https:\/\/doi.org\/10.1002\/biot.202300285","journal-title":"Biotechnol J"},{"key":"5041_CR30","doi-asserted-by":"publisher","first-page":"1050740","DOI":"10.3389\/fbioe.2022.1050740","volume":"10","author":"HB Singh","year":"2022","unstructured":"Singh HB, Kang MK, Kwon M, Kim SW (2022a) Developing methylotrophic microbial platforms for a methanol-based bioindustry. Front Bioeng Biotechnol 10:1050740. https:\/\/doi.org\/10.3389\/fbioe.2022.1050740","journal-title":"Front Bioeng Biotechnol"},{"key":"5041_CR29","doi-asserted-by":"publisher","first-page":"101717","DOI":"10.1016\/j.fbio.2022.101717","volume":"47","author":"RV Singh","year":"2022","unstructured":"Singh RV, Sambyal K (2022b) An overview of \u03b2-carotene production: Current status and future prospects. Food Biosci 47:101717. https:\/\/doi.org\/10.1016\/j.fbio.2022.101717","journal-title":"Food Biosci"},{"key":"5041_CR32","doi-asserted-by":"publisher","first-page":"107768","DOI":"10.1016\/j.biotechadv.2021.107768","volume":"50","author":"I S\u00fcntar","year":"2021","unstructured":"S\u00fcntar I, \u00c7etinkaya S, Haydaro\u011flu \u00dcS, Habtemariam S (2021) Bioproduction process of natural products and biopharmaceuticals: Biotechnological aspects. Biotechnol Adv 50:107768. https:\/\/doi.org\/10.1016\/j.biotechadv.2021.107768","journal-title":"Biotechnol Adv"},{"key":"5041_CR31","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1016\/j.psep.2022.03.060","volume":"161","author":"Y Sun","year":"2022","unstructured":"Sun Y, Qin Z, Tang Y, Liao C, Liu Y, Ma X (2022) Techno-environmental-economic assessment on municipal solid waste to methanol coupling with\/without solid oxygen electrolysis cell unit. Process Saf Environ Prot 161:611\u2013623. https:\/\/doi.org\/10.1016\/j.psep.2022.03.060","journal-title":"Process Saf Environ Prot"},{"key":"5041_CR33","doi-asserted-by":"publisher","first-page":"4342","DOI":"10.1128\/AEM.02759-06","volume":"73","author":"R Verwaal","year":"2007","unstructured":"Verwaal R, Wang J, Meijnen JP, Visser H, Sandmann G, van den Berg JA, van Ooyen AJJ (2007) High-Level Production of beta-carotene in Saccharomyces cerevisiae by successive Transformation with Carotenogenic Genes from Xanthophyllomyces dendrorhous. Appl Environ Microbiol 73:4342\u20134350. https:\/\/doi.org\/10.1128\/AEM.02759-06","journal-title":"Appl Environ Microbiol"},{"issue":"3","key":"5041_CR34","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/S1567-1356(03)00158-2","volume":"4","author":"H Visser","year":"2003","unstructured":"Visser H, van Ooyen AJJ, Verdoes JC (2003) Metabolic engineering of the astaxanthin-biosynthetic pathway of Xanthophyllomyces dendrorhous. FEMS Yeast Res 4(3):221\u2013231. https:\/\/doi.org\/10.1016\/S1567-1356(03)00158-2","journal-title":"FEMS Yeast Res"},{"key":"5041_CR53","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1021\/acssynbio.5b00199","volume":"5","author":"T Vogl","year":"2016","unstructured":"Vogl T, Sturmberger L, Kickenweiz T, Wasmayer R, Schmid C, Hatzl A-M, Gerstmann MA, Pitzer J, Wagner M, Thallinger GG, Geier M, Glieder A (2016) A toolbox of diverse promoters related to methanol utilization: functionally verified parts for heterologous pathway expression in Pichia pastoris. ACS Synth Biol 5:172\u2013186. https:\/\/doi.org\/10.1021\/acssynbio.5b00199","journal-title":"ACS Synth Biol"},{"key":"5041_CR36","doi-asserted-by":"publisher","first-page":"36329","DOI":"10.1039\/D1RA06253K","volume":"11","author":"D Wang","year":"2021","unstructured":"Wang D, Chen M, Zeng X, Li W, Liang S, Lin Y (2021a) Improving the catalytic performance of Pichia pastoris whole-cell biocatalysts by fermentation process. RSC Adv 11:36329\u201336339. https:\/\/doi.org\/10.1039\/D1RA06253K","journal-title":"RSC Adv"},{"key":"5041_CR39","doi-asserted-by":"publisher","first-page":"1952","DOI":"10.1021\/acs.jafc.4c10113","volume":"73","author":"J Wang","year":"2025","unstructured":"Wang J, Ma W, Ma W, Yao Z, Jiang Y, Jiang W, Xin F, Zhang W, Jiang M (2025) Microbial Astaxanthin synthesis by Komagataella phaffii through metabolic and fermentation engineering. J Agric Food Chem 73:1952\u20131964. https:\/\/doi.org\/10.1021\/acs.jafc.4c10113","journal-title":"J Agric Food Chem"},{"key":"5041_CR37","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/j.tifs.2021b.02.077","volume":"111","author":"L Wang","year":"2021","unstructured":"Wang L, Liu Z, Jiang H, Mao X (2021b) Biotechnology advances in \u03b2-carotene production by microorganisms. Trends Food Sci Technol 111:322\u2013332. https:\/\/doi.org\/10.1016\/j.tifs.2021b.02.077","journal-title":"Trends Food Sci Technol"},{"key":"5041_CR38","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1021\/acsfoodscitech.4c00023","volume":"4","author":"N Wang","year":"2024","unstructured":"Wang N, Guo W, Yang C, Wang K, Li G, Liu D (2024) Improved Astaxanthin synthesis in Komagataella phaffii through \u03b2-carotene ketolase screening and \u03b2-carotene hydroxylase mutagenesis. ACS Food Sci Technol 4:975\u2013982. https:\/\/doi.org\/10.1021\/acsfoodscitech.4c00023","journal-title":"ACS Food Sci Technol"},{"key":"5041_CR35","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1016\/j.tibtech.2019.12.013","volume":"38","author":"Y Wang","year":"2020","unstructured":"Wang Y, Fan L, Tuyishime P, Zheng P, Sun J (2020) Synthetic methylotrophy: A practical solution for methanol-based biomanufacturing. Trends Biotechnol 38:650\u2013666. https:\/\/doi.org\/10.1016\/j.tibtech.2019.12.013","journal-title":"Trends Biotechnol"},{"key":"5041_CR40","doi-asserted-by":"publisher","first-page":"152","DOI":"10.2144\/04361DD02","volume":"36","author":"S Wu","year":"2004","unstructured":"Wu S, Letchworth GJ (2004) High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol. BioTechniques 36:152\u2013154. https:\/\/doi.org\/10.2144\/04361DD02","journal-title":"BioTechniques"},{"key":"5041_CR41","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00585","volume":"8","author":"Y Wu","year":"2020","unstructured":"Wu Y, Yan P, Li Y, Liu X, Wang Z, Chen T, Zhao X (2020) Enhancing \u03b2-carotene production in Escherichia coli by perturbing central carbon metabolism and improving the NADPH supply. Front Bioeng Biotechnol 8:585. https:\/\/doi.org\/10.3389\/fbioe.2020.00585","journal-title":"Front Bioeng Biotechnol"},{"key":"5041_CR42","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1002\/bit.25002","volume":"111","author":"W Xie","year":"2014","unstructured":"Xie W, Liu M, Lv X, Lu W, Gu J, Yu H (2014) Construction of a controllable \u03b2-carotene biosynthetic pathway by decentralized assembly strategy in Saccharomyces cerevisiae. Biotechnol Bioeng 111:125\u2013133. https:\/\/doi.org\/10.1002\/bit.25002","journal-title":"Biotechnol Bioeng"},{"key":"5041_CR45","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-024-04037-4","volume":"40","author":"R Yamada","year":"2024","unstructured":"Yamada R, Ando K, Sakaguchi R, Matsumoto T, Ogino H (2024) Induction of point and structural mutations in engineered yeast Saccharomyces cerevisiae improve carotenoid production. World J Microbiol Biotechnol 40:230. https:\/\/doi.org\/10.1007\/s11274-024-04037-4","journal-title":"World J Microbiol Biotechnol"},{"key":"5041_CR44","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-019-2610-4","volume":"35","author":"R Yamada","year":"2019","unstructured":"Yamada R, Ogura K, Kimoto Y, Ogino H (2019) Toward the construction of a technology platform for chemicals production from methanol: d-lactic acid production from methanol by an engineered yeast Pichia pastoris. World J Microbiol Biotechnol 35:37. https:\/\/doi.org\/10.1007\/s11274-019-2610-4","journal-title":"World J Microbiol Biotechnol"},{"key":"5041_CR43","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1016\/j.biortech.2018.08.044","volume":"268","author":"R Yamada","year":"2018","unstructured":"Yamada R, Yamauchi A, Ando Y, Kumata Y, Ogino H (2018) Modulation of gene expression by cocktail \u03b4-integration to improve carotenoid production in Saccharomyces cerevisiae. Bioresour Technol 268:616\u2013621. https:\/\/doi.org\/10.1016\/j.biortech.2018.08.044","journal-title":"Bioresour Technol"},{"key":"5041_CR46","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1016\/j.jbiotec.2009.01.008","volume":"140","author":"SH Yoon","year":"2009","unstructured":"Yoon SH, Lee SH, Das A, Ryu HK, Jang HJ, Kim JY, Oh DK, Keasling JD, Kim SW (2009) Combinatorial expression of bacterial whole mevalonate pathway for the production of \u03b2-carotene in E. coli. J Biotechnol 140:218\u2013226. https:\/\/doi.org\/10.1016\/j.jbiotec.2009.01.008","journal-title":"J Biotechnol"},{"key":"5041_CR47","doi-asserted-by":"publisher","DOI":"10.3390\/jof9101027","volume":"9","author":"J Zha","year":"2023","unstructured":"Zha J, Liu D, Ren J, Liu Z, Wu X (2023) Advances in metabolic engineering of Pichia pastoris strains as powerful cell factories. J Fungi 9:1027. https:\/\/doi.org\/10.3390\/jof9101027","journal-title":"J Fungi"},{"key":"5041_CR48","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1038\/s41929-023-00957-w","volume":"6","author":"C Zhan","year":"2023","unstructured":"Zhan C, Li X, Lan G, Baidoo EEK, Yang Y, Liu Y, Sun Y, Wang S, Wang Y, Wang G, Nielsen J, Keasling JD, Chen Y, Bai Z (2023) Reprogramming methanol utilization pathways to convert Saccharomyces cerevisiae to a synthetic methylotroph. Nat Catal 6:435\u2013450. https:\/\/doi.org\/10.1038\/s41929-023-00957-w","journal-title":"Nat Catal"},{"key":"5041_CR49","doi-asserted-by":"publisher","first-page":"2170","DOI":"10.1016\/j.biotechadv.2018.09.001","volume":"36","author":"W Zhang","year":"2018","unstructured":"Zhang W, Zhang T, Song M, Dai Z, Zhang W, Guo Y, Liu D (2018) Current advance in bioconversion of methanol to chemicals. Biotechnol Adv 36:2170\u20132185. https:\/\/doi.org\/10.1016\/j.biotechadv.2018.09.001","journal-title":"Biotechnol Adv"},{"key":"5041_CR50","doi-asserted-by":"publisher","first-page":"2961","DOI":"10.1021\/acssynbio.3c00294","volume":"12","author":"X Zhang","year":"2023","unstructured":"Zhang X, Chen S, Lin Y, Li W, Wang D, Ruan S, Yang Y, Liang S (2023) Metabolic engineering of Pichia pastoris for high-level production of lycopene. ACS Synth Biol 12:2961\u20132972. https:\/\/doi.org\/10.1021\/acssynbio.3c00294","journal-title":"ACS Synth Biol"},{"key":"5041_CR51","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1111\/lam.12463","volume":"61","author":"X Zhao","year":"2015","unstructured":"Zhao X, Shi F, Zhan W (2015) Overexpression of ZWF1 and POS5 improves carotenoid biosynthesis in recombinant Saccharomyces cerevisiae. Lett Appl Microbiol 61:354\u2013360. https:\/\/doi.org\/10.1111\/lam.12463","journal-title":"Lett Appl Microbiol"},{"key":"5041_CR52","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2025.03.013","volume":"90","author":"W Zhou","year":"2025","unstructured":"Zhou W, Ling RJ, Yang YC, Yang Y, Hou ST, Wang FQ, Gao B, Wei DZ (2025) Engineering Komagataella phaffii to produce lycopene sustainably from glucose or methanol. Metab Eng 90:141. https:\/\/doi.org\/10.1016\/j.ymben.2025.03.013","journal-title":"Metab Eng"}],"container-title":["World Journal of Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-026-05041-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11274-026-05041-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-026-05041-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T11:17:36Z","timestamp":1781954256000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11274-026-05041-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,27]]},"references-count":53,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["5041"],"URL":"https:\/\/doi.org\/10.1007\/s11274-026-05041-6","relation":{},"ISSN":["1573-0972"],"issn-type":[{"value":"1573-0972","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,27]]},"assertion":[{"value":"3 October 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 May 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"305"}}