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Here, we present a comprehensive genomic and phenotypic characterization of\n                    <jats:italic>P. sacchari<\/jats:italic>\n                    strain LMG 19450\n                    <jats:sup>T<\/jats:sup>\n                    and compare it with three additional\n                    <jats:italic>P. sacchari<\/jats:italic>\n                    genomes and phylogenetically related\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    species. A high-quality genome assembly confirmed its placement within the\n                    <jats:italic>Paraburkholderia<\/jats:italic>\n                    clade and species-level relatedness among\n                    <jats:italic>P. sacchari<\/jats:italic>\n                    strains despite detectable intraspecies genomic variability. Pangenome analysis revealed a large, conserved core genome and an extensive accessory gene repertoire, consistent with adaptation to plant-associated soil environments. Comparative analysis of virulence-associated genes showed a marked contrast between\n                    <jats:italic>P. sacchari<\/jats:italic>\n                    and pathogenic\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    , with the former lacking most canonical pathogenicity determinants. Gene tree\u2013species tree reconciliation further indicated a constrained horizontal gene transfer profile for virulence-associated gene families, while genes involved in transcriptional regulation displayed higher transfer propensity. Antimicrobial resistance profiling revealed a limited resistome dominated by efflux-associated homologs, consistent with broad phenotypic susceptibility. Notably, strain LMG 19450\n                    <jats:sup>T<\/jats:sup>\n                    exhibited antibiotic susceptibility patterns comparable to those of the established microbial chassis\n                    <jats:italic>Pseudomonas putida<\/jats:italic>\n                    KT2440. Virulence assessment using the\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    model demonstrated markedly attenuated pathogenicity relative to\n                    <jats:italic>Burkholderia cepacia<\/jats:italic>\n                    . Together, these findings support\n                    <jats:italic>P. sacchari<\/jats:italic>\n                    as an environmentally adapted bacterium with a favorable biosafety profile and highlight the value of integrating genomic and experimental approaches to evaluate emerging microbial chassis.\n                  <\/jats:p>","DOI":"10.1007\/s11274-026-05227-y","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:17:25Z","timestamp":1790407045000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated genomic and experimental assessment of the biosafety of Paraburkholderia sacchari as a promising microbial chassis"],"prefix":"10.1007","volume":"42","author":[{"given":"Matheus Marques","family":"Torres","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Henrique Diniz","family":"Gervasio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric Andr\u00e9 Velasco","family":"Y\u00e9pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Silva","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele Muniz","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Luisa","family":"Zardo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Cardinali-Rezende","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Welington Luiz","family":"de Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Greg\u00f3rio Cabrera","family":"Gomez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiziana Ferreira","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,26]]},"reference":[{"key":"5227_CR1","doi-asserted-by":"publisher","unstructured":"Alexandrino PMR, Mendon\u00e7a TT, Guam\u00e1n Bautista LP, Cherix J, Lozano-Sakalauskas GC, Fujita A, Ramos Filho E, Long P, Padilla G, Taciro MK, Gomez JGC, Silva LF (2015) Draft genome sequence of the polyhydroxyalkanoate-producing bacterium burkholderia sacchari LMG 19450 Isolated from Brazilian sugarcane plantation soil. 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