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We report an electrospinning-based formulation that encapsulates\n                    <jats:italic>Bacillus amyloliquefaciens<\/jats:italic>\n                    fermentation broth within poly(vinyl alcohol) (PVA) nanofibers to enhance storage stability and preserve microbial viability. Electrospun nanofiber mats were obtained from PVA formulations containing the fermentation broth, and their morphology, chemical profile, mechanical properties, and microbial viability were evaluated. Morphological, spectroscopic, mechanical, and microbiological analyses demonstrated successful incorporation of the fermentation broth into the PVA matrix and retention of viable\n                    <jats:italic>Bacillus<\/jats:italic>\n                    cells after electrospinning, with comparative estimates of viable cell recovery of 3.3\n                    <jats:bold>\u2009\u00d7\u2009<\/jats:bold>\n                    10\n                    <jats:sup>7<\/jats:sup>\n                    CFU cm\n                    <jats:sup>\u2212\u20092<\/jats:sup>\n                    in the electrospun fibers and 8.3\u2009\u00d7\u200910\n                    <jats:sup>7<\/jats:sup>\n                    CFU mL\n                    <jats:sup>\u2212\u20091<\/jats:sup>\n                    in the polymeric spinning solution before electrospinning. Nanofibers stored under ambient conditions for up to one year still yielded viable cells. Collectively, these findings support the potential of electrospun PVA nanofibers as a formulation strategy for microbial stabilization and warrant further investigation toward the development of microbial formulations with improved storage characteristics.\n                  <\/jats:p>","DOI":"10.1007\/s11274-026-05334-w","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T08:01:15Z","timestamp":1791532875000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Electrospun PVA nanofibers improve the storage stability of Bacillus amyloliquefaciens fermentation broth"],"prefix":"10.1007","volume":"42","author":[{"given":"Paula Derksen","family":"Macruz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna Picoli","family":"Libel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Haruo Nakayama","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliano Cesar","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio Ant\u00f4nio","family":"Barraca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Radovanovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Jorge","family":"Pilau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"key":"5334_CR1","doi-asserted-by":"publisher","DOI":"10.14719\/pst.9087","author":"R Adhithiya Rajah","year":"2025","unstructured":"Adhithiya Rajah R, Raja K, Johnson I et al (2025) Electrospinning applications in sustainable agriculture: Enhancing soil health, seed coatings and post-harvest antimicrobial protection. 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