{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:40:40Z","timestamp":1792701640733,"version":"4.5.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"11","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2103200"],"award-info":[{"award-number":["2021YFC2103200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22578390"],"award-info":[{"award-number":["22578390"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["World J Microbiol Biotechnol"],"DOI":"10.1007\/s11274-026-05361-7","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:16:34Z","timestamp":1792653394000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High efficient production of adenine in Bacillus subtilis with novel metabolic engineering strategies"],"prefix":"10.1007","volume":"42","author":[{"given":"Shuang","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peijie","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijun","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangli","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhinan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"key":"5361_CR1","doi-asserted-by":"publisher","first-page":"5421","DOI":"10.1128\/AEM.01453-16","volume":"82","author":"J Altenbuchner","year":"2016","unstructured":"Altenbuchner J (2016) Editing of the Bacillus subtilis Genome by the CRISPR-Cas9System. Appl Environ Microbiol 82:5421\u20135427. https:\/\/doi.org\/10.1128\/AEM.01453-16","journal-title":"Appl Environ Microbiol"},{"key":"5361_CR2","doi-asserted-by":"publisher","first-page":"6615","DOI":"10.1021\/bi060287y","volume":"45","author":"S Arent","year":"2006","unstructured":"Arent S, Kadziola A, Larsen S et al (2006) The extraordinary specificity of xanthine phosphoribosyltransferase from Bacillus subtilis elucidated by reaction kinetics, ligand binding, and crystallography,. Biochemistry 45:6615\u20136627. https:\/\/doi.org\/10.1021\/bi060287y","journal-title":"Biochemistry"},{"key":"5361_CR3","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1016\/j.bbapap.2014.01.010","volume":"1844","author":"B Arivett","year":"2014","unstructured":"Arivett B, Farone M, Masiragani R et al (2014) Characterization of inosine\u2013uridine nucleoside hydrolase (RihC) from Escherichia coli. Biochimica et Biophysica Acta (BBA) -. Proteins Proteom 1844:656\u2013662. https:\/\/doi.org\/10.1016\/j.bbapap.2014.01.010","journal-title":"Proteins Proteom"},{"key":"5361_CR4","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1186\/s13068-022-02179-x","volume":"15","author":"A Deng","year":"2022","unstructured":"Deng A, Qiu Q, Sun Q et al (2022) In silico-guided metabolic engineering of Bacillus subtilis for efficient biosynthesis of purine nucleosides by blocking the key backflow nodes. Biotechnol Biofuels 15:82. https:\/\/doi.org\/10.1186\/s13068-022-02179-x","journal-title":"Biotechnol Biofuels"},{"key":"5361_CR5","doi-asserted-by":"publisher","first-page":"8274","DOI":"10.1016\/S0021-9258(18)47560-6","volume":"262","author":"DJ Ebbole","year":"1987","unstructured":"Ebbole DJ, Zalkin H (1987) Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis. J Biol Chem 262:8274\u20138287. https:\/\/doi.org\/10.1016\/S0021-9258(18)47560-6","journal-title":"J Biol Chem"},{"issue":"4","key":"5361_CR6","doi-asserted-by":"publisher","first-page":"2136","DOI":"10.1128\/jb.171.4.2136-2141.1989","volume":"171","author":"DJ Ebbole","year":"1989","unstructured":"Ebbole DJ, Zalkin H (1989) Bacillus subtilis pur operon expression and regulationt. J BACTERIOL 171(4):2136\u20132141. https:\/\/doi.org\/10.1128\/jb.171.4.2136-2141.1989","journal-title":"J BACTERIOL"},{"key":"5361_CR7","doi-asserted-by":"publisher","first-page":"3165","DOI":"10.1111\/febs.16746","volume":"290","author":"A Gedeon","year":"2023","unstructured":"Gedeon A, Karimova G, Ayoub N et al (2023) Interaction network among de novo purine nucleotide biosynthesis enzymes in Escherichia coli. FEBS J 290:3165\u20133184. https:\/\/doi.org\/10.1111\/febs.16746","journal-title":"FEBS J"},{"key":"5361_CR8","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.ymben.2018.05.006","volume":"50","author":"Y Gu","year":"2018","unstructured":"Gu Y, Xu XH, Wu YK et al (2018) Advances and prospects of Bacillus subtilis cellular factories: From rational design to industrial applications. Metab Eng 50:109\u2013121. https:\/\/doi.org\/10.1016\/j.ymben.2018.05.006","journal-title":"Metab Eng"},{"key":"5361_CR9","doi-asserted-by":"publisher","unstructured":"He B, Shiau A, Choi KY et al (1990) Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction. 172. https:\/\/doi.org\/10.1128\/jb.172.8.4555-4562.1990","DOI":"10.1128\/jb.172.8.4555-4562.1990"},{"key":"5361_CR10","doi-asserted-by":"publisher","unstructured":"He B, Smith JM, Zalkin H (1992) Escherichia coli purB Gene: cloning, nucleotide sequence, and regulation by purR. J Bacteriol 174(1):130\u2013136. https:\/\/doi.org\/10.1128\/jb.174.1.130-136.1992","DOI":"10.1128\/jb.174.1.130-136.1992"},{"key":"5361_CR11","doi-asserted-by":"publisher","first-page":"718","DOI":"10.13343\/j.cnki.wsxb.2012.06.013","volume":"52","author":"KF He","year":"2012","unstructured":"He KF, Ma YC, Du SS, Xie XX (2012) Effects of overexpression of key enzyme genes on guanosine accumulation in Bacillus amyloliquefaciens. Acta Microbiol Sinica 52:718\u2013725. https:\/\/doi.org\/10.13343\/j.cnki.wsxb.2012.06.013","journal-title":"Acta Microbiol Sinica"},{"key":"5361_CR12","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.jsb.2019.02.007","volume":"206","author":"P-A Kang","year":"2019","unstructured":"Kang P-A, Oh J, Lee H et al (2019) Crystal structure and mutational analyses of ribokinase from Arabidopsis thaliana. J Struct Biol 206:110\u2013118. https:\/\/doi.org\/10.1016\/j.jsb.2019.02.007","journal-title":"J Struct Biol"},{"key":"5361_CR13","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1016\/S1367-5931(00)00133-2","volume":"4","author":"TJ Kappock","year":"2000","unstructured":"Kappock TJ, Ealick SE, Stubbe J (2000) Modular evolution of the purine biosynthetic pathway. Curr Opin Chem Biol 4:567\u2013572. https:\/\/doi.org\/10.1016\/S1367-5931(00)00133-2","journal-title":"Curr Opin Chem Biol"},{"key":"5361_CR15","doi-asserted-by":"publisher","first-page":"1575","DOI":"10.1007\/s10529-011-0597-5","volume":"33","author":"H Li","year":"2011","unstructured":"Li H, Zhang G, Deng A et al (2011) De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis. Biotechnol Lett 33:1575\u20131580. https:\/\/doi.org\/10.1007\/s10529-011-0597-5","journal-title":"Biotechnol Lett"},{"key":"5361_CR14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jbiotec.2019.04.007","volume":"298","author":"B Li","year":"2019","unstructured":"Li B, Yan Z-Y, Liu X-N et al (2019) Increased fermentative adenosine production by gene-targeted Bacillus subtilis mutation. J Biotechnol 298:1\u20134. https:\/\/doi.org\/10.1016\/j.jbiotec.2019.04.007","journal-title":"J Biotechnol"},{"key":"5361_CR16","doi-asserted-by":"publisher","first-page":"1675","DOI":"10.1007\/s10529-017-2412-4","volume":"39","author":"Y Liao","year":"2017","unstructured":"Liao Y, Ye Y, Wang B, Pan L (2017) Optimization of the purine operon and energy generation in Bacillus amyloliquefaciens for guanosine production. Biotechnol Lett 39:1675\u20131682. https:\/\/doi.org\/10.1007\/s10529-017-2412-4","journal-title":"Biotechnol Lett"},{"issue":"6","key":"5361_CR17","first-page":"641","volume":"54","author":"Y liu","year":"2014","unstructured":"liu Y, He JH, Xie XX, Xu QY (2014) Modification in de novo purine pathway for adenosine accumulation by Bacillus subtilis. Acta Microbiol Sinica 54(6):641\u2013647","journal-title":"Acta Microbiol Sinica"},{"key":"5361_CR18","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1134\/S1022795411070131","volume":"47","author":"KV Lobanov","year":"2011","unstructured":"Lobanov KV, Korol\u2019kova NV, Eremina SY et al (2011) Mutation analysis of the purine operon leader region in Bacillus subtilis. Russ J Genet 47:785\u2013793. https:\/\/doi.org\/10.1134\/S1022795411070131","journal-title":"Russ J Genet"},{"key":"5361_CR19","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1128\/jb.158.2.665-673.1984","volume":"158","author":"JE Lopilato","year":"1984","unstructured":"Lopilato JE, Garwin JL, Emr SD et al (1984) D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. J Bacteriol 158:665\u2013673. https:\/\/doi.org\/10.1128\/jb.158.2.665-673.1984","journal-title":"J Bacteriol"},{"key":"5361_CR20","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1016\/S0092-8674(03)00391-X","volume":"113","author":"M Mandal","year":"2003","unstructured":"Mandal M, Boese B, Barrick JE et al (2003) Riboswitches control fundamental biochemical pathways in Bacillus subtilis and other bacteria. Cell 113:577\u2013586. https:\/\/doi.org\/10.1016\/S0092-8674(03)00391-X","journal-title":"Cell"},{"key":"5361_CR21","doi-asserted-by":"publisher","first-page":"7816","DOI":"10.1021\/bi800329p","volume":"47","author":"M Morar","year":"2008","unstructured":"Morar M, Hoskins AA, Stubbe J, Ealick SE (2008) Formylglycinamide Ribonucleotide Amidotransferase from Thermotoga maritima: Structural Insights into Complex Formation. Biochemistry 47:7816\u20137830. https:\/\/doi.org\/10.1021\/bi800329p","journal-title":"Biochemistry"},{"key":"5361_CR22","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1007\/s00253-011-3687-3","volume":"93","author":"P Natalia","year":"2012","unstructured":"Natalia P, Zakataeva DV, Romanenkov VS et al (2012) Wild-type and feedback-resistant phosphoribosyl pyrophosphate synthetases from Bacillus amyloliquefaciens: purification, characterization, and application to increase purine nucleoside production. Appl Microbiol Biotechnol 93:2023\u20132033. https:\/\/doi.org\/10.1007\/s00253-011-3687-3","journal-title":"Appl Microbiol Biotechnol"},{"issue":"10","key":"5361_CR23","doi-asserted-by":"publisher","first-page":"2538","DOI":"10.1111\/trf.13740","volume":"56","author":"G Paglia","year":"2016","unstructured":"Paglia G, Sigurj\u00f3nsson \u00d3E, Bordbar A, Rolfsson \u00d3 (2016) Metabolic fate of adenine in red blood cells during storage in SAGM solution. Transfusion 56(10):2538\u20132547. https:\/\/doi.org\/10.1111\/trf.13740","journal-title":"Transfusion"},{"key":"5361_CR24","doi-asserted-by":"publisher","first-page":"2875","DOI":"10.1007\/s00018-008-8123-1","volume":"65","author":"J Park","year":"2008","unstructured":"Park J, Gupta RS (2008) Adenosine kinase and ribokinase \u2013 the RK family of proteins. Cell Mol Life Sci 65:2875\u20132896. https:\/\/doi.org\/10.1007\/s00018-008-8123-1","journal-title":"Cell Mol Life Sci"},{"key":"5361_CR25","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1186\/1475-2859-11-138","volume":"11","author":"S Peifer","year":"2012","unstructured":"Peifer S, Barduhn T, Zimmet S et al (2012) Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine. Microb Cell Fact 11:138. https:\/\/doi.org\/10.1186\/1475-2859-11-138","journal-title":"Microb Cell Fact"},{"key":"5361_CR26","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1074\/jbc.M008300200","volume":"276","author":"C Petersen","year":"2001","unstructured":"Petersen C, M\u00f8ller LB (2001) The RihA, RihB, and RihC Ribonucleoside Hydrolases of Escherichia coli. J Biol Chem 276:884\u2013894. https:\/\/doi.org\/10.1074\/jbc.M008300200","journal-title":"J Biol Chem"},{"key":"5361_CR27","doi-asserted-by":"publisher","first-page":"3810","DOI":"10.1128\/JB.181.12.3810-3815.1999","volume":"181","author":"P Rappu","year":"1999","unstructured":"Rappu P, Shin BS, Zalkin H, M\u00e4nts\u00e4l\u00e4 P (1999) A Role for a Highly Conserved Protein of Unknown Function in Regulation of Bacillus subtilis purA by the Purine Repressor. J Bacteriol 181:3810\u20133815. https:\/\/doi.org\/10.1128\/JB.181.12.3810-3815.1999","journal-title":"J Bacteriol"},{"key":"5361_CR28","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1042\/BST0340786","volume":"34","author":"RJ Rolfes","year":"2006","unstructured":"Rolfes RJ (2006) Regulation of purine nucleotide biosynthesis: in yeast and beyond. Biochem Soc Trans 34:786\u2013790. https:\/\/doi.org\/10.1042\/BST0340786","journal-title":"Biochem Soc Trans"},{"key":"5361_CR29","doi-asserted-by":"publisher","first-page":"6175","DOI":"10.1128\/JB.183.21.6175-6183.2001","volume":"183","author":"HH Saxild","year":"2001","unstructured":"Saxild HH, Brunstedt K, Nielsen KI et al (2001) Definition of the Bacillus subtilis PurR Operator Using Genetic and Bioinformatic Tools and Expansion of the PurR Regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO. J Bacteriol 183:6175\u20136183. https:\/\/doi.org\/10.1128\/JB.183.21.6175-6183.2001","journal-title":"J Bacteriol"},{"key":"5361_CR30","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1186\/s12934-014-0101-8","volume":"13","author":"T Shi","year":"2014","unstructured":"Shi T, Wang Y, Wang Z et al (2014) Deregulation of purine pathway in Bacillus subtilis and its use in riboflavin biosynthesis. Microb Cell Fact 13:101. https:\/\/doi.org\/10.1186\/s12934-014-0101-8","journal-title":"Microb Cell Fact"},{"key":"5361_CR31","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1263\/jbb.103.255","volume":"103","author":"M Shimaoka","year":"2007","unstructured":"Shimaoka M, Takenaka Y, Kurahashi O et al (2007) Effect of amplification of desensitized purF and prs on inosine accumulation in Escherichia coli. J Biosci Bioeng 103:255\u2013261. https:\/\/doi.org\/10.1263\/jbb.103.255","journal-title":"J Biosci Bioeng"},{"key":"5361_CR32","doi-asserted-by":"publisher","first-page":"4087","DOI":"10.1128\/JB.185.14.4087-4098.2003","volume":"185","author":"SC Sinha","year":"2003","unstructured":"Sinha SC, Krahn J, Shin BS et al (2003) The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation. J Bacteriol 185:4087\u20134098. https:\/\/doi.org\/10.1128\/JB.185.14.4087-4098.2003","journal-title":"J Bacteriol"},{"key":"5361_CR33","doi-asserted-by":"publisher","first-page":"157","DOI":"10.9758\/cpn.2015.13.2.157","volume":"13","author":"I Takeuchi","year":"2015","unstructured":"Takeuchi I, Kishi T, Hanya M et al (2015) Effect of Adenine on Clozapine-induced Neutropenia in Patients with Schizophrenia: A Preliminary Study. Clin Psychopharmacol Neurosci 13:157\u2013162. https:\/\/doi.org\/10.9758\/cpn.2015.13.2.157","journal-title":"Clin Psychopharmacol Neurosci"},{"key":"5361_CR34","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1111\/j.1742-4658.2006.05155.x","volume":"273","author":"MG Tozzi","year":"2006","unstructured":"Tozzi MG, Camici M, Mascia L et al (2006) Pentose phosphates in nucleoside interconversion and catabolism. FEBS J 273:1089\u20131101. https:\/\/doi.org\/10.1111\/j.1742-4658.2006.05155.x","journal-title":"FEBS J"},{"key":"5361_CR35","doi-asserted-by":"publisher","first-page":"7455","DOI":"10.1073\/pnas.92.16.7455","volume":"92","author":"M Weng","year":"1995","unstructured":"Weng M, Nagy PL, Zalkin H (1995) Identification of the Bacillus subtilis pur operon repressor. Proc Natl Acad Sci USA 92:7455\u20137459. https:\/\/doi.org\/10.1073\/pnas.92.16.7455","journal-title":"Proc Natl Acad Sci USA"},{"key":"5361_CR36","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.synbio.2020.07.005","volume":"5","author":"MG Xiang","year":"2020","unstructured":"Xiang MG, Kang Q, Zhang DW (2020) Advances on systems metabolic engineering of Bacillus subtilis as a chassis cell. Synth Syst Biotechnol 5:245\u2013251. https:\/\/doi.org\/10.1016\/j.synbio.2020.07.005","journal-title":"Synth Syst Biotechnol"},{"key":"5361_CR37","doi-asserted-by":"publisher","first-page":"1361","DOI":"10.1007\/s10529-015-1801-9","volume":"37","author":"C Zhang","year":"2015","unstructured":"Zhang C, Du S, Liu Y et al (2015) Strategy for enhancing adenosine production under the guidance of transcriptional and metabolite pool analysis. Biotechnol Lett 37:1361\u20131369. https:\/\/doi.org\/10.1007\/s10529-015-1801-9","journal-title":"Biotechnol Lett"},{"key":"5361_CR38","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1186\/s12934-024-02576-x","volume":"23","author":"S Zhao","year":"2024","unstructured":"Zhao S, Shi T, Li L et al (2024) The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microb Cell Fact 23:309. https:\/\/doi.org\/10.1186\/s12934-024-02576-x","journal-title":"Microb Cell Fact"},{"key":"5361_CR39","doi-asserted-by":"publisher","first-page":"20210","DOI":"10.1021\/acs.jafc.3c07715","volume":"71","author":"M Zhou","year":"2023","unstructured":"Zhou M, Li Y, Cai Y et al (2023) Development of an Inosine Hyperproducer from Bacillus licheniformis by Systems Metabolic Engineering. J Agric Food Chem 71:20210\u201320221. https:\/\/doi.org\/10.1021\/acs.jafc.3c07715","journal-title":"J Agric Food Chem"}],"container-title":["World Journal of Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-026-05361-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11274-026-05361-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11274-026-05361-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:03:26Z","timestamp":1792699406000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11274-026-05361-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,22]]},"references-count":39,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2026,11]]}},"alternative-id":["5361"],"URL":"https:\/\/doi.org\/10.1007\/s11274-026-05361-7","relation":{},"ISSN":["1573-0972"],"issn-type":[{"value":"1573-0972","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,22]]},"assertion":[{"value":"26 November 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 October 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"620"}}