{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T17:13:12Z","timestamp":1762881192561,"version":"3.45.0"},"reference-count":82,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T00:00:00Z","timestamp":1740355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T00:00:00Z","timestamp":1740355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Purinergic Signalling"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1007\/s11302-025-10076-9","type":"journal-article","created":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T01:01:20Z","timestamp":1740358880000},"page":"1135-1144","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["First evidence of Tityus confluens Borelli, 1899 (Buthidae) venom altering purine metabolism in rat blood cells"],"prefix":"10.1007","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8193-865X","authenticated-orcid":false,"given":"Rom\u00e1rio","family":"da Silva Portilho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0660-8861","authenticated-orcid":false,"given":"Igor Leal","family":"Brito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1339-3889","authenticated-orcid":false,"given":"Andreza Negreli","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5352-4893","authenticated-orcid":false,"given":"Bruna Pache","family":"Moreschi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2566-5642","authenticated-orcid":false,"given":"Malson Neilson","family":"de Lucena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2498-1013","authenticated-orcid":false,"given":"Jeandre Augusto","family":"Otsubo Jaques","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,2,24]]},"reference":[{"key":"10076_CR1","first-page":"1","volume":"13","author":"ACM Gomes","year":"2022","unstructured":"Gomes ACM et al (2022) Escorpi\u00f5es do g\u00eanero Tityus no Brasil: biologia, bioqu\u00edmica da pe\u00e7onha e fisiopatologia do escorpionismo. Scientia Vitae 13:1\u201314","journal-title":"Scientia Vitae"},{"key":"10076_CR2","doi-asserted-by":"publisher","first-page":"172","DOI":"10.22354\/in.v26i2.1018","volume":"26","author":"AFB Parrela","year":"2022","unstructured":"Parrela AFB et al (2022) Scorpion envenomation in Brazil: an update. Infectio 26:172\u201318. https:\/\/doi.org\/10.22354\/in.v26i2.1018","journal-title":"Infectio"},{"key":"10076_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1590\/1678-4766e2017108","volume":"107","author":"LS Carvalho","year":"2017","unstructured":"Carvalho LS et al (2017) Checklist dos escorpi\u00f5es (Arachnida, Scorpiones) do Mato Grosso do Sul. Brasil Iheringia S\u00e9rie Zoologia 107:1\u20137. https:\/\/doi.org\/10.1590\/1678-4766e2017108","journal-title":"Brasil Iheringia S\u00e9rie Zoologia"},{"key":"10076_CR4","volume-title":"Os Escorpi\u00f5es","author":"TK Brazil","year":"2010","unstructured":"Brazil TK, Porto TJ (2010) Os Escorpi\u00f5es. EDUFBA, Salvador"},{"key":"10076_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2017.00984","volume":"8","author":"WM Santussi","year":"2017","unstructured":"Santussi WM et al (2017) Antifungal activity against filamentous fungi of Ts1, a multifunctional toxin from Tityus serrulatus scorpion venom. Front Microbiol 8:1\u201315. https:\/\/doi.org\/10.3389\/fmicb.2017.00984","journal-title":"Front Microbiol"},{"key":"10076_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2019\/5131042","volume":"2019","author":"E Bernardes-Oliveira","year":"2019","unstructured":"Bernardes-Oliveira E et al (2019) Tityus serrulatus scorpion venom induces apoptosis in cervical cancer cell lines. Evid Based Complement Alternat Med 2019:1\u20138. https:\/\/doi.org\/10.1155\/2019\/5131042","journal-title":"Evid Based Complement Alternat Med"},{"key":"10076_CR7","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.toxicon.2018.07.024","volume":"152","author":"MF Martin-Eauclaire","year":"2018","unstructured":"Martin-Eauclaire MF, Bougis PE, Lima ME (2018) Ts1 from the Brazilian scorpion Tityus serrulatus: A half-century of studies on a multifunctional beta like-toxin. Toxicon 152:106\u2013120. https:\/\/doi.org\/10.1016\/j.toxicon.2018.07.024","journal-title":"Toxicon"},{"key":"10076_CR8","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1016\/j.toxicon.2015.10.015","volume":"108","author":"MB Pucca","year":"2015","unstructured":"Pucca MB et al (2015) Tityus serrulatus venom \u2013 A lethal cocktail. Toxicon 108:272\u2013284. https:\/\/doi.org\/10.1016\/j.toxicon.2015.10.015","journal-title":"Toxicon"},{"key":"10076_CR9","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1016\/j.toxicon.2011.08.006","volume":"58","author":"EMS Fialho","year":"2011","unstructured":"Fialho EMS et al (2011) Immune cells recruitment and activation by Tityus serrulatus scorpion venom. Toxicon 58:480\u2013485. https:\/\/doi.org\/10.1016\/j.toxicon.2011.08.006","journal-title":"Toxicon"},{"key":"10076_CR10","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.cca.2005.05.012","volume":"141","author":"F Vasconcelos","year":"2005","unstructured":"Vasconcelos F et al (2005) Effects of voltage-gated Na+ channel toxins from Tityus serrulatus venom on rat arterial blood pressure and plasma catecholamines. Comp Biochem Physiol C Toxicol Pharmacol 141:85\u201392. https:\/\/doi.org\/10.1016\/j.cca.2005.05.012","journal-title":"Comp Biochem Physiol C Toxicol Pharmacol"},{"key":"10076_CR11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/s0014-2999(97)01110-2","volume":"333","author":"J Sawynok","year":"1997","unstructured":"Sawynok J et al (1997) Adenosine A3 receptor activation produces nociceptive behaviour and edema by release of histamine and 5-hydroxytryptamine. Eur J Pharmacol 333:1\u20137. https:\/\/doi.org\/10.1016\/s0014-2999(97)01110-2","journal-title":"Eur J Pharmacol"},{"key":"10076_CR12","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/s0014-2999(00)00125-4","volume":"395","author":"J Sawynok","year":"2000","unstructured":"Sawynok J, Reid A, Liu XJ (2000) Involvement of mast cells, sensory afferents and sympathetic mechanisms in paw edema induced by adenosine A(1) and A(2B\/3) receptor agonists. Eur J Pharmacol 395:47\u201350. https:\/\/doi.org\/10.1016\/s0014-2999(00)00125-4","journal-title":"Eur J Pharmacol"},{"key":"10076_CR13","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1007\/s00134-020-05924-8","volume":"46","author":"F Abroug","year":"2020","unstructured":"Abroug F et al (2020) Scorpion envenomation: state of the art. Intensive Care Med 46:401\u2013410. https:\/\/doi.org\/10.1007\/s00134-020-05924-8","journal-title":"Intensive Care Med"},{"key":"10076_CR14","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1016\/s0041-0101(01)00232-x","volume":"40","author":"SD Aird","year":"2002","unstructured":"Aird SD (2002) Ophidian envenomation strategies and the role of purines. Toxicon 40:335\u2013393. https:\/\/doi.org\/10.1016\/s0041-0101(01)00232-x","journal-title":"Toxicon"},{"key":"10076_CR15","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.cbpc.2007.08.003","volume":"147","author":"PBV Sales","year":"2008","unstructured":"Sales PBV, Santoro ML (2008) Nucleotidase and DNase activities in Brazilian snake venoms. Comp Biochem Physiol C toxicol Pharmacol 147:85\u201395. https:\/\/doi.org\/10.1016\/j.cbpc.2007.08.003","journal-title":"Comp Biochem Physiol C toxicol Pharmacol"},{"key":"10076_CR16","doi-asserted-by":"publisher","first-page":"1471","DOI":"10.1007\/s00018-007-6497-0","volume":"64","author":"G Burnstock","year":"2007","unstructured":"Burnstock G (2007) Purine and pyrimidine receptors. Cell Mol Life Sci 64:1471\u20131483. https:\/\/doi.org\/10.1007\/s00018-007-6497-0","journal-title":"Cell Mol Life Sci"},{"key":"10076_CR17","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.bcmd.2005.12.025","volume":"36","author":"B Atkinson","year":"2006","unstructured":"Atkinson B et al (2006) Ecto-nucleotidases of the CD39\/NTPDase family modulate platelet activation and thrombus formation: Potential as therapeutic targets. Blood Cells Mol Dis 36:217\u2013222. https:\/\/doi.org\/10.1016\/j.bcmd.2005.12.025","journal-title":"Blood Cells Mol Dis"},{"key":"10076_CR18","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1111\/j.1538-7836.2010.03769.x","volume":"8","author":"D Yang","year":"2010","unstructured":"Yang D et al (2010) A new role for the A2b adenosine receptor in regulating platelet function. J Thromb Haemost 8:817\u2013827. https:\/\/doi.org\/10.1111\/j.1538-7836.2010.03769.x","journal-title":"J Thromb Haemost"},{"key":"10076_CR19","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1146\/annurev-immunol-051116-052406","volume":"37","author":"J Linden","year":"2019","unstructured":"Linden J, Koch-Nolte F, Dahl G (2019) Purine release, metabolism, and signaling in the inflammatory response. Annu Rev Immunol 37:325\u2013347. https:\/\/doi.org\/10.1146\/annurev-immunol-051116-052406","journal-title":"Annu Rev Immunol"},{"key":"10076_CR20","doi-asserted-by":"publisher","first-page":"3454","DOI":"10.1016\/j.febslet.2015.07.027","volume":"589","author":"MR Bono","year":"2015","unstructured":"Bono MR et al (2015) CD73 and CD39 ectonucleotidases in T cell differentiation: Beyond immunosuppression. FEBS lett 589:3454\u20133460. https:\/\/doi.org\/10.1016\/j.febslet.2015.07.027","journal-title":"FEBS lett"},{"key":"10076_CR21","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1182\/blood-2013-02-482406","volume":"122","author":"Z Saze","year":"2013","unstructured":"Saze Z et al (2013) Adenosine production by human B cells and B cell-mediated suppression of activated T cells. Blood 122:9\u201318. https:\/\/doi.org\/10.1182\/blood-2013-02-482406","journal-title":"Blood"},{"key":"10076_CR22","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1038\/nri.2016.4","volume":"16","author":"C Cekic","year":"2016","unstructured":"Cekic C, Linden J (2016) Purinergic regulation of the immune system. Nat Rev Immunol 16:177\u2013192. https:\/\/doi.org\/10.1038\/nri.2016.4","journal-title":"Nat Rev Immunol"},{"key":"10076_CR23","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.autneu.2015.04.011","volume":"191","author":"F Di Virgilio","year":"2015","unstructured":"Di Virgilio F, Vuerich M (2015) Purinergic signaling in the immune system. Auton Neurosci 191:117\u2013123. https:\/\/doi.org\/10.1016\/j.autneu.2015.04.011","journal-title":"Auton Neurosci"},{"key":"10076_CR24","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1182\/blood.v97.3.587","volume":"97","author":"F Di Virgilio","year":"2001","unstructured":"Di Virgilio F et al (2001) Nucleotide receptors: an emerging family of regulatory molecules in blood cells. Blood 97:587\u2013600. https:\/\/doi.org\/10.1182\/blood.v97.3.587","journal-title":"Blood"},{"key":"10076_CR25","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1038\/s41568-018-0037-0","volume":"18","author":"F Di Virgilio","year":"2018","unstructured":"Di Virgilio F et al (2018) Extracellular ATP and P2 purinergic signalling in the tumour microenvironment. Nat Rev Cancer 18:601\u20136018. https:\/\/doi.org\/10.1038\/s41568-018-0037-0","journal-title":"Nat Rev Cancer"},{"key":"10076_CR26","doi-asserted-by":"publisher","DOI":"10.7476\/9786586545494","volume-title":"Sinaliza\u00e7\u00e3o purin\u00e9rgica: implica\u00e7\u00f5es fisiopatol\u00f3gicas","author":"AM Cardoso","year":"2021","unstructured":"Cardoso AM, Manfredi LH, Maciel SFVO (2021) Sinaliza\u00e7\u00e3o purin\u00e9rgica: implica\u00e7\u00f5es fisiopatol\u00f3gicas. Editora UFFS, Brasil"},{"key":"10076_CR27","doi-asserted-by":"publisher","first-page":"473","DOI":"10.3109\/10409238.2014.953627","volume":"49","author":"GG Yegutkin","year":"2014","unstructured":"Yegutkin GG (2014) Enzymes involved in metabolism of extracellular nucleotides and nucleosides: Functional implications and measurement of activities. Crit Rev Biochem Mol Biol 49:473\u2013497. https:\/\/doi.org\/10.3109\/10409238.2014.953627","journal-title":"Crit Rev Biochem Mol Biol"},{"key":"10076_CR28","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1007\/s11302-013-9398-8","volume":"10","author":"G Burnstock","year":"2014","unstructured":"Burnstock G, Vaughn B, Robson SC (2014) Purinergic signalling in the liver in health and disease. Purinergic Signal 10:51\u201370. https:\/\/doi.org\/10.1007\/s11302-013-9398-8","journal-title":"Purinergic Signal"},{"key":"10076_CR29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.bcp.2020.114322","volume":"187","author":"H Zimmermann","year":"2021","unstructured":"Zimmermann H (2021) History of ectonucleotidases and their role in purinergic signaling. Biochem Pharmacol 187:1\u201377. https:\/\/doi.org\/10.1016\/j.bcp.2020.114322","journal-title":"Biochem Pharmacol"},{"key":"10076_CR30","doi-asserted-by":"publisher","first-page":"2024","DOI":"10.3390\/ijms15022024","volume":"15","author":"S Sheth","year":"2014","unstructured":"Sheth S et al (2014) Adenosine receptors: expression, function and regulation. Int J Mol Sci 15:2024\u20132052. https:\/\/doi.org\/10.3390\/ijms15022024","journal-title":"Int J Mol Sci"},{"key":"10076_CR31","doi-asserted-by":"publisher","unstructured":"Burnstock G (2006). Purinergic signalling - An overview. Novartis Found Symp 26\u201353. https:\/\/doi.org\/10.1002\/9780470032244.ch4","DOI":"10.1002\/9780470032244.ch4"},{"key":"10076_CR32","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1038\/nri2938","volume":"11","author":"WG Junger","year":"2011","unstructured":"Junger WG (2011) Immune cell regulation by autocrine purinergic signalling. Nat Rev Immunol 11:201\u2013212. https:\/\/doi.org\/10.1038\/nri2938","journal-title":"Nat Rev Immunol"},{"key":"10076_CR33","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1038\/s41392-021-00553-z","volume":"6","author":"Z Huang","year":"2021","unstructured":"Huang Z et al (2021) From purines to purinergic signalling: molecular functions and human diseases. Signal Transduct Target Ther 6:162\u2013182. https:\/\/doi.org\/10.1038\/s41392-021-00553-z","journal-title":"Signal Transduct Target Ther"},{"key":"10076_CR34","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.neuron.2012.09.024","volume":"76","author":"BS Khakh","year":"2012","unstructured":"Khakh BS, North RA (2012) Neuromodulation by extracellular ATP and P2X receptors in the CNS. Neuron 76:51\u201369. https:\/\/doi.org\/10.1016\/j.neuron.2012.09.024","journal-title":"Neuron"},{"key":"10076_CR35","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1124\/mol.114.092528","volume":"86","author":"S Jones","year":"2014","unstructured":"Jones S, Evans RJ, Mahaut-smith MP (2014) Ca2+ influx through P2X1 receptors amplifies P2Y1 receptor-evoked Ca2+ signaling and ADP-evoked platelet aggregation. Mol Pharmacol 86:243\u2013251. https:\/\/doi.org\/10.1124\/mol.114.092528","journal-title":"Mol Pharmacol"},{"key":"10076_CR36","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1002\/cbf.1637","volume":"28","author":"BL Dhananjaya","year":"2010","unstructured":"Dhananjaya BL, D\u2019souza CJM (2010) The pharmacological role of nucleotidases in snake venoms. Cell Biochem Funct 28:171\u2013177. https:\/\/doi.org\/10.1002\/cbf.1637","journal-title":"Cell Biochem Funct"},{"key":"10076_CR37","doi-asserted-by":"publisher","first-page":"247","DOI":"10.3390\/toxins11050247","volume":"11","author":"JI Cid-uribe","year":"2019","unstructured":"Cid-uribe JI et al (2019) Dissecting Toxicity: the venom gland transcriptome and the venom proteome of the highly venomous scorpion Centruroides limpidus (karsch, 1879). Toxins 11:247\u2013268. https:\/\/doi.org\/10.3390\/toxins11050247","journal-title":"Toxins"},{"key":"10076_CR38","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1016\/s1074-5521(99)80085-4","volume":"6","author":"SJ Stachel","year":"1999","unstructured":"Stachel SJ, Stockwell SA, Vranken DLV (1999) The fluorescence of scorpions and cataractogenesis. Chem Biol 6:531\u2013539. https:\/\/doi.org\/10.1016\/s1074-5521(99)80085-4","journal-title":"Chem Biol"},{"key":"10076_CR39","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1186\/s40409-015-0046-3","volume":"21","author":"A Casella-martins","year":"2015","unstructured":"Casella-martins A et al (2015) Immunomodulatory activity of Tityus serrulatus scorpion venom on human T lymphocytes. J Venom Anim Toxins Incl Trop Dis 21:46\u201354. https:\/\/doi.org\/10.1186\/s40409-015-0046-3","journal-title":"J Venom Anim Toxins Incl Trop Dis"},{"key":"10076_CR40","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-020-19232-8","volume":"11","author":"MB Reis","year":"2020","unstructured":"Reis MB et al (2020) Interleukin-1 receptor-induced PGE2 production controls acetylcholine-mediated cardiac dysfunction and mortality during scorpion envenomation. Nat Commun 11:1\u201312. https:\/\/doi.org\/10.1038\/s41467-020-19232-8","journal-title":"Nat Commun"},{"key":"10076_CR41","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.neuropharm.2019.04.013","volume":"153","author":"RC Rita","year":"2019","unstructured":"Rita RC et al (2019) Scorpion venom increases acetylcholine release by prolonging the duration of somatic nerve action potentials. Neuropharmacology 153:41\u201352. https:\/\/doi.org\/10.1016\/j.neuropharm.2019.04.013","journal-title":"Neuropharmacology"},{"key":"10076_CR42","first-page":"77","volume":"97","author":"A Boyum","year":"1968","unstructured":"Boyum A (1968) Isolation of mononuclear cells and granulocytes from human blood, Isolation on mononuclear cells by centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1g. Scand J Clin Lab Invest Suppl 97:77\u201389","journal-title":"Scand J Clin Lab Invest Suppl"},{"key":"10076_CR43","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.bbagen.2004.09.006","volume":"1721","author":"DB Leal","year":"2005","unstructured":"Leal DB et al (2005) Characterization of NTPDase (NTPDase1; ecto-apyrase; ecto-diphosphohydrolase; CD39; EC 3.6.1.5) activity in human lymphocytes. Biochim Biophys Acta 1721:9\u201315. https:\/\/doi.org\/10.1016\/j.bbagen.2004.09.006","journal-title":"Biochim Biophys Acta"},{"key":"10076_CR44","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1111\/j.1471-4159.1984.tb12832.x","volume":"43","author":"D Heymann","year":"1984","unstructured":"Heymann D, Reddington M, Kreutzberg GW (1984) Subcellular localization of 5\u2019-nucleotidase in rat brain. J Neurochem 43:971\u2013978. https:\/\/doi.org\/10.1111\/j.1471-4159.1984.tb12832.x","journal-title":"J Neurochem"},{"key":"10076_CR45","doi-asserted-by":"publisher","first-page":"225","DOI":"10.3109\/09537109609023582","volume":"7","author":"C Pilla","year":"1996","unstructured":"Pilla C et al (1996) ATP diphosphohydrolase (apyrase, EC 3.6.1.5) in human blood platelets. Platelets 7:225\u2013230. https:\/\/doi.org\/10.3109\/09537109609023582","journal-title":"Platelets"},{"key":"10076_CR46","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1006\/abio.1976.9999","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248\u2013254. https:\/\/doi.org\/10.1006\/abio.1976.9999","journal-title":"Anal Biochem"},{"key":"10076_CR47","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/0003-2697(86)90640-8","volume":"157","author":"KM Chan","year":"1986","unstructured":"Chan KM et al (1986) A direct colorimetric assay for Ca2+ stimulated ATPase activity. Anal Biochem 157:375\u2013380. https:\/\/doi.org\/10.1016\/0003-2697(86)90640-8","journal-title":"Anal Biochem"},{"key":"10076_CR48","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1159\/000459567","volume":"12","author":"G Giusti","year":"1971","unstructured":"Giusti G, Gakis C (1971) Temperature conversion factors, activation energy, relative substrate specificity and optimum pH of adenosine deaminase from human serum and tissues. Enzyme 12:417\u2013425. https:\/\/doi.org\/10.1159\/000459567","journal-title":"Enzyme"},{"key":"10076_CR49","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1590\/1414-431X2020e10850","volume":"54","author":"LR Costa","year":"2021","unstructured":"Costa LR et al (2021) Biochemical characterization of adenosine deaminase (CD26; EC 3.5.4.4) activity in human lymphocyte-rich peripheral blood mononuclear cells. Braz J Med Biol Res 54:1\u20137. https:\/\/doi.org\/10.1590\/1414-431X2020e10850","journal-title":"Braz J Med Biol Res"},{"key":"10076_CR50","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/0003-9861(76)90327-1","volume":"174","author":"CR Geren","year":"1976","unstructured":"Geren CR et al (1976) Isolation and characterization of toxin from Brown reclusa spider venom (Loxosceles recluse). Arch Biochem Biophys 174:90\u201399. https:\/\/doi.org\/10.1016\/0003-9861(76)90327-1","journal-title":"Arch Biochem Biophys"},{"key":"10076_CR51","doi-asserted-by":"publisher","first-page":"207","DOI":"10.3390\/toxins10050207","volume":"10","author":"C Santib\u00e1\u00f1ez-l\u00f3pez","year":"2018","unstructured":"Santib\u00e1\u00f1ez-l\u00f3pez C et al (2018) Transcriptomic analysis of pseudoscorpion venom reveals a unique cocktail dominated by enzymes and protease inhibitors. Toxins 10:207\u2013219. https:\/\/doi.org\/10.3390\/toxins10050207","journal-title":"Toxins"},{"key":"10076_CR52","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1590\/1678-9199-JVATITD-1482-18","volume":"25","author":"FG Amorim","year":"2019","unstructured":"Amorim FG et al (2019) Proteome of fraction from Tityus serrulatus venom reveals new enzymes and toxins. J Venom Anim Toxins Incl Trop Dis 25:1\u201311. https:\/\/doi.org\/10.1590\/1678-9199-JVATITD-1482-18","journal-title":"J Venom Anim Toxins Incl Trop Dis"},{"key":"10076_CR53","doi-asserted-by":"publisher","first-page":"27","DOI":"10.22179\/revmacn.23.714","volume":"23","author":"AAO Affilastro","year":"2021","unstructured":"Affilastro AAO et al (2021) Redefinition of the identity and phylogenetic position of Tityus trivittatus Kraepelin 1898, and description of Tityus carrilloi n. sp. (Scorpiones; Buthidae), the most medically important scorpion of southern South America. Rev Mus Argent Cienc Nat 23:27\u201355. https:\/\/doi.org\/10.22179\/revmacn.23.714","journal-title":"Rev Mus Argent Cienc Nat"},{"key":"10076_CR54","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/j.neuroscience.2006.02.010","volume":"140","author":"E Arrigoni","year":"2006","unstructured":"Arrigoni E et al (2006) Adenosine inhibits basal forebrain cholinergic and noncholinergic neurons in vitro. Neuroscience 140:403\u2013413. https:\/\/doi.org\/10.1016\/j.neuroscience.2006.02.010","journal-title":"Neuroscience"},{"key":"10076_CR55","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1146\/annurev.neuro.24.1.31","volume":"24","author":"TV Dunwiddie","year":"2001","unstructured":"Dunwiddie TV, Masino SA (2001) The role and regulation of adenosine in the central nervous system. Annu Rev Neurosci 24:31\u201355. https:\/\/doi.org\/10.1146\/annurev.neuro.24.1.31","journal-title":"Annu Rev Neurosci"},{"key":"10076_CR56","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.cbpc.2004.09.020","volume":"140","author":"SD Aird","year":"2005","unstructured":"Aird SD (2005) Taxonomic distribution and quantitative analysis of free purine and pyrimidine nucleosides in snake venoms. Comp Biochem Physiol B Biochem Mol Biol 140:109\u2013126. https:\/\/doi.org\/10.1016\/j.cbpc.2004.09.020","journal-title":"Comp Biochem Physiol B Biochem Mol Biol"},{"key":"10076_CR57","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1038\/sj.bjp.0706062","volume":"144","author":"IM Concei\u00e7\u00e3o","year":"2005","unstructured":"Concei\u00e7\u00e3o IM et al (2005) Selective release of ATP from sympathetic nerves of rat vas deferens by the toxin TsTX-I from Brazilian scorpion Tityus serrulatus. Br J Pharmacol 144:519\u2013527. https:\/\/doi.org\/10.1038\/sj.bjp.0706062","journal-title":"Br J Pharmacol"},{"key":"10076_CR58","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2010\/903295","volume":"2010","author":"VL Petricevich","year":"2010","unstructured":"Petricevich VL (2010) Scorpion venom and the inflammatory response. Mediators Inflamm 2010:1\u201316. https:\/\/doi.org\/10.1155\/2010\/903295","journal-title":"Mediators Inflamm"},{"key":"10076_CR59","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/0163-7258(94)00048-4","volume":"64","author":"MP Abbracchio","year":"1994","unstructured":"Abbracchio MP, Burnstock G (1994) Purinoceptors: Are there families of P2X and P2Y purinoceptors? Pharmacol Ther 64:445\u2013475. https:\/\/doi.org\/10.1016\/0163-7258(94)00048-4","journal-title":"Pharmacol Ther"},{"key":"10076_CR60","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1152\/physrev.00015.2002","volume":"82","author":"RA North","year":"2002","unstructured":"North RA (2002) Molecular physiology of P2X receptors. Physiol Rev 82:1013\u20131067. https:\/\/doi.org\/10.1152\/physrev.00015.2002","journal-title":"Physiol Rev"},{"key":"10076_CR61","doi-asserted-by":"publisher","first-page":"442","DOI":"10.4025\/revcivet.v6i2.46911","volume":"6","author":"J Brites-neto","year":"2019","unstructured":"Brites-neto J (2019) Aspectos cl\u00ednicos e terap\u00eauticos do envenenamento por escorpi\u00f5es em c\u00e3es e gatos. Rev Ci\u00eanc Vet Sa\u00fade P\u00fablica 6:442\u2013471. https:\/\/doi.org\/10.4025\/revcivet.v6i2.46911","journal-title":"Rev Ci\u00eanc Vet Sa\u00fade P\u00fablica"},{"key":"10076_CR62","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1177\/2398212818817494","volume":"2","author":"G Burnstock","year":"2018","unstructured":"Burnstock G (2018) Purine and purinergic receptors. Brain Neurosci Adv 2:1\u201310. https:\/\/doi.org\/10.1177\/2398212818817494","journal-title":"Brain Neurosci Adv"},{"key":"10076_CR63","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.toxicon.2020.11.452","volume":"190","author":"B Oehler","year":"2021","unstructured":"Oehler B et al (2021) Effects of recombinant botulinum neurotoxin type A1 on CFA-induced mechanical allodynia and sensory neuron responses to mechanical stimulation monitored with GCaMP fluorescence in mice. Toxicon 190:52\u201353. https:\/\/doi.org\/10.1016\/j.toxicon.2020.11.452","journal-title":"Toxicon"},{"key":"10076_CR64","doi-asserted-by":"publisher","first-page":"407","DOI":"10.4149\/gpb_2019026","volume":"38","author":"J Zhou","year":"2019","unstructured":"Zhou J et al (2019) Involvement of P2X7 receptors in satellite glial cells of dorsal root ganglia in the bmk i-induced pain model of rats. Gen Physiol Biophys 38:407\u2013416. https:\/\/doi.org\/10.4149\/gpb_2019026","journal-title":"Gen Physiol Biophys"},{"key":"10076_CR65","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1007\/s12264-019-00345-0","volume":"35","author":"J Zhou","year":"2019","unstructured":"Zhou J et al (2019) Up-regulation of P2X7 receptors contributes to spinal microglial activation and the development of pain induced by BmK-I. Neurosci Bull 35:624\u2013636. https:\/\/doi.org\/10.1007\/s12264-019-00345-0","journal-title":"Neurosci Bull"},{"key":"10076_CR66","doi-asserted-by":"publisher","first-page":"920","DOI":"10.2174\/092986609788923329","volume":"16","author":"C Cologna","year":"2009","unstructured":"Cologna C et al (2009) Tityus serrulatus scorpion venom and toxins: an overview. Protein Pept Lett 16:920\u2013932. https:\/\/doi.org\/10.2174\/092986609788923329","journal-title":"Protein Pept Lett"},{"key":"10076_CR67","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1189\/jlb.1a0514-267rr","volume":"99","author":"M Barber\u00e1-cremades","year":"2016","unstructured":"Barber\u00e1-cremades M, Baroja-mazo A, Pelegr\u00edn P (2016) Purinergic signaling during macrophage differentiation results in M2 alternative activated macrophages. J Leukoc Biol 99:289\u2013299. https:\/\/doi.org\/10.1189\/jlb.1a0514-267rr","journal-title":"J Leukoc Biol"},{"key":"10076_CR68","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1016\/j.toxicon.2004.12.020","volume":"45","author":"EB Nascimento","year":"2005","unstructured":"Nascimento EB et al (2005) Pharmacological investigation of the nociceptive response and edema induced by venom of the scorpion Tityus serrulatus. Toxicon 45:585\u2013593. https:\/\/doi.org\/10.1016\/j.toxicon.2004.12.020","journal-title":"Toxicon"},{"key":"10076_CR69","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1080\/09629350210308","volume":"11","author":"VL Petricevich","year":"2002","unstructured":"Petricevich VL (2002) Effect of Tityus serrulatus venom on cytokine production and the activity of murine macrophages. Mediators Inflamm 11:23\u201331. https:\/\/doi.org\/10.1080\/09629350210308","journal-title":"Mediators Inflamm"},{"key":"10076_CR70","unstructured":"Guimar\u00e3es P (2009) Caracteriza\u00e7\u00e3o molecular e imunol\u00f3gica do veneno de Tityus fasciolatus e sua a\u00e7\u00e3o sobre camundongos. PhD. Thesis. Universidade Federal de Minas Gerais (UFMG, Brazil)"},{"key":"10076_CR71","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1007\/s11302-006-9042-y","volume":"3","author":"S Gessi","year":"2007","unstructured":"Gessi S et al (2007) Adenosine and lymphocyte regulation. Purinergic Signal 3:109\u2013116. https:\/\/doi.org\/10.1007\/s11302-006-9042-y","journal-title":"Purinergic Signal"},{"key":"10076_CR72","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1111\/imm.13744","volume":"171","author":"T Zhang","year":"2024","unstructured":"Zhang T et al (2024) Adenosine 2A receptor antagonists promote lymphocyte proliferation in sepsis by inhibiting Treg expression of PD-L1 in spleen. Immunology 171:566\u2013582. https:\/\/doi.org\/10.1111\/imm.13744","journal-title":"Immunology"},{"key":"10076_CR73","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.imlet.2017.02.002","volume":"183","author":"G Jo\u00f3s","year":"2017","unstructured":"Jo\u00f3s G et al (2017) Involvement of adenosine A3 receptors in the chemotactic navigation of macrophages towards apoptotic cells. Immunol Lett 183:62\u201372. https:\/\/doi.org\/10.1016\/j.imlet.2017.02.002","journal-title":"Immunol Lett"},{"key":"10076_CR74","doi-asserted-by":"publisher","first-page":"7685","DOI":"10.3390\/ijms22147685","volume":"22","author":"S Pasquini","year":"2021","unstructured":"Pasquini S et al (2021) Adenosine and inflammation: here, there and everywhere. Int J Mol Sci 22:7685\u20137709. https:\/\/doi.org\/10.3390\/ijms22147685","journal-title":"Int J Mol Sci"},{"key":"10076_CR75","doi-asserted-by":"publisher","first-page":"1591","DOI":"10.1152\/physrev.00049.2017","volume":"98","author":"PA Borea","year":"2018","unstructured":"Borea PA et al (2018) Pharmacology of adenosine receptors: the state of the art. Physiol Rev 98:1591\u20131625. https:\/\/doi.org\/10.1152\/physrev.00049.2017","journal-title":"Physiol Rev"},{"key":"10076_CR76","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fphar.2018.00037","volume":"9","author":"M Koupenova","year":"2018","unstructured":"Koupenova M, Ravid K (2018) Biology of platelet purinergic receptors and implications for platelet heterogeneity. Front Pharmacol 9:1\u20139. https:\/\/doi.org\/10.3389\/fphar.2018.00037","journal-title":"Front Pharmacol"},{"key":"10076_CR77","doi-asserted-by":"publisher","first-page":"902","DOI":"10.1080\/09537104.2021.1902972","volume":"32","author":"C Oury","year":"2021","unstructured":"Oury C, W\u00e9ra O (2021) P2X1: a unique platelet receptor with a key role in thromboinflammation. Platelets 32:902\u2013908. https:\/\/doi.org\/10.1080\/09537104.2021.1902972","journal-title":"Platelets"},{"key":"10076_CR78","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1538-7836.2010.03769.x","volume":"8","author":"D Yang","year":"2010","unstructured":"Yang D et al (2010) A new role for the A2b adenosine receptor in regulating platelet function. J Thromb Haemost 8:1\u20138. https:\/\/doi.org\/10.1111\/j.1538-7836.2010.03769.x","journal-title":"J Thromb Haemost"},{"key":"10076_CR79","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1590\/S0074-02762005000900019","volume":"100","author":"L Harrington","year":"2005","unstructured":"Harrington L, Mitchell J (2005) P2X1 receptors and the endothelium. Mem Inst Oswaldo Cruz 100:111\u2013112. https:\/\/doi.org\/10.1590\/S0074-02762005000900019","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10076_CR80","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1007\/s002100000309","volume":"362","author":"H Zimmermann","year":"2000","unstructured":"Zimmermann H (2000) Extracellular metabolism of ATP and other nucleotides. Naunyn Schmiedebergs Arch Pharmacol 362:299\u2013309. https:\/\/doi.org\/10.1007\/s002100000309","journal-title":"Naunyn Schmiedebergs Arch Pharmacol"},{"key":"10076_CR81","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1007\/s11302-020-09755-6","volume":"17","author":"H Zimmermann","year":"2021","unstructured":"Zimmermann H (2021) Ectonucleoside triphosphate diphosphohydrolases and ecto-5\u2032-nucleotidase in purinergic signaling: how the field developed and where we are now. Purinergic Signal 17:117\u2013125. https:\/\/doi.org\/10.1007\/s11302-020-09755-6","journal-title":"Purinergic Signal"},{"key":"10076_CR82","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1016\/j.pharep.2015.05.002","volume":"67","author":"P Zukowska","year":"2015","unstructured":"Zukowska P et al (2015) The role of ecto-5\u2032-nucleotidase in endothelial dysfunction and vascular pathologies. Pharmacol Rep 67:675\u2013681. https:\/\/doi.org\/10.1016\/j.pharep.2015.05.002","journal-title":"Pharmacol Rep"}],"container-title":["Purinergic Signalling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11302-025-10076-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11302-025-10076-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11302-025-10076-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T17:09:47Z","timestamp":1762880987000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11302-025-10076-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,24]]},"references-count":82,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10076"],"URL":"https:\/\/doi.org\/10.1007\/s11302-025-10076-9","relation":{},"ISSN":["1573-9538","1573-9546"],"issn-type":[{"type":"print","value":"1573-9538"},{"type":"electronic","value":"1573-9546"}],"subject":[],"published":{"date-parts":[[2025,2,24]]},"assertion":[{"value":"14 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 February 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 February 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The animal study was reviewed and approved by the Animal Research Ethics Committee at the Federal University of Mato Grosso do Sul (CEUA\/UFMS).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}