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However, the structure of the microbial community within endometriotic lesions and its impact on the microenvironment is still limited.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>All 55 tissue samples, including ovarian ectopic (OEMs) and normal (NE) endometrium, were subjected to 16S rRNA sequencing, metabolomic and proteomic analysis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We found the abundance of <jats:italic>Tuzzerella<\/jats:italic> is significantly lower in OEMs compared to NE tissue (p\u2009&lt;\u20090.01). We selected samples from these two groups that exhibited the most pronounced difference in <jats:italic>Tuzzerella<\/jats:italic> abundance for further metabolomic and proteomic analysis. Our findings indicated that endometriotic lesions were associated with a decrease in <jats:sc>l<\/jats:sc>-Glutamine levels. However, proteomic analysis revealed a significant upregulation of proteins related to the complement pathway, including C3, C7, C1S, CLU, and A2M. Subsequent metabolic and protein correlation predictions demonstrated a negative regulation between <jats:sc>l<\/jats:sc>-Glutamine and C7. In vitro experiments further confirmed that high concentrations of Glutamine significantly inhibit C7 protein expression. Additionally, immune cell infiltration analysis, multiplex immunofluorescence, and multifactorial testing demonstrated a positive correlation between C7 expression and the infiltration of regulatory T cells (Tregs) in ectopic lesions, while <jats:sc>l<\/jats:sc>-Glutamine was found to negatively regulate the expression of chemotactic factors for Tregs.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>In this study, we found a clear multi-omics pathway alteration, \u201c<jats:italic>Tuzzerella<\/jats:italic> (microbe)\u2014<jats:sc>l<\/jats:sc>-Glutamine (metabolite)\u2014C7 (protein),\u201d which affects the infiltration of Tregs in endometriotic lesions. Our findings provide insights into endometriosis classification and personalized treatment strategies based on microbial structures.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1007\/s11306-023-02072-0","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T20:02:01Z","timestamp":1709236921000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Disrupted Tuzzerella abundance and impaired l-glutamine levels induce Treg accumulation in ovarian endometriosis: a comprehensive multi-omics analysis"],"prefix":"10.1007","volume":"20","author":[{"given":"Yichen","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingfang","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jue","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhui","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yishen","family":"Rong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,29]]},"reference":[{"key":"2072_CR1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.21330","author":"L Araujo","year":"2017","unstructured":"Araujo, L., Khim, P., Mkhikian, H., Mortales, C. 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