{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:02:45Z","timestamp":1788346965592,"version":"build-2803163510"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T00:00:00Z","timestamp":1740009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T00:00:00Z","timestamp":1740009600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Helmholtz Zentrum M\u00fcnchen - Deutsches Forschungszentrum f\u00fcr Gesundheit und Umwelt (GmbH)"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Metabolomics"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Introduction<\/jats:title>\n            <jats:p>The identification of lipids is a cornerstone of lipidomics, and due to the specific characteristics of lipids, it requires dedicated analysis workflows. Identifying novel lipids and lipid species for which no reference spectra are available is tedious and often involves a lot of manual work. Integrating high-resolution mass spectrometry with enhancements from chromatographic and ion mobility separation enables the in-depth investigation of intact lipids.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Objectives<\/jats:title>\n            <jats:p>We investigated phosphorylated glycosphingolipids from the nematode <jats:italic>Caenorhabditis elegans<\/jats:italic>, a biomedical model organism, and aimed to identify different species from this class of lipids, which have been described in one particular publication only. We checked if these lipids can be detected in lipid extracts of <jats:italic>C. elegans<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>We used UHPLC-UHR-TOF-MS and UHPLC-TIMS-TOF-MS in combination with dedicated data analysis to check for the presence of phosphorylated glycosphingolipids. Specifically, candidate features were identified in two datasets using Mass Spec Query Language (MassQL) to search fragmentation data. The additional use of retention time (RT) and collisional cross section (CCS) information allowed to filter false positive annotations.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>As a result, we detected all previously described phosphorylated glycosphingolipids and novel species as well as their biosynthetic precursors in two different lipidomics datasets. MassQL significantly speeds up the process by saving time that would otherwise be spent on manual data investigations. In total over 20 sphingolipids could be described.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>MassQL allowed us to search for phosphorylated glycosphingolipids and their potential biosynthetic precursors systematically. Using orthogonal information such as RT and CCS helped filter false positive results. With the detection in two different datasets, we demonstrate that these sphingolipids are a general part of the <jats:italic>C. elegans<\/jats:italic> lipidome.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s11306-024-02216-w","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T17:31:15Z","timestamp":1740072675000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Phosphorylated glycosphingolipids are commonly detected in Caenorhabditis elegans lipidomes"],"prefix":"10.1007","volume":"21","author":[{"given":"Michael","family":"Witting","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liesa","family":"Salzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven W.","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiko","family":"Barsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,2,20]]},"reference":[{"key":"2216_CR1","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/j.jpha.2022.06.006","volume":"12","author":"NH Anh","year":"2022","unstructured":"Anh, N. H., Yoon, Y. C., Min, Y. J., Long, N. P., Jung, C. W., Kim, S. J., Kim, S. W., Lee, E. G., Wang, D., Wang, X., & Kwon, S. W. (2022). Caenorhabditis elegans deep lipidome profiling by using integrative mass spectrometry acquisitions reveals significantly altered lipid networks. Journal of Pharmaceutical Analysis, 12, 743\u2013754.","journal-title":"Journal of Pharmaceutical Analysis"},{"key":"2216_CR2","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1139\/y59-099","volume":"37","author":"EG Bligh","year":"1959","unstructured":"Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37, 911\u2013917.","journal-title":"Canadian Journal of Biochemistry and Physiology"},{"key":"2216_CR3","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1038\/nchembio.2347","volume":"13","author":"S Boland","year":"2017","unstructured":"Boland, S., Schmidt, U., Zagoriy, V., Sampaio, J. L., Fritsche, R. F., Czerwonka, R., L\u00fcbken, T., Reimann, J., Penkov, S., Kn\u00f6lker, H. J., & Kurzchalia, T. V. (2017). Phosphorylated glycosphingolipids essential for cholesterol mobilization in Caenorhabditis elegans. Nature Chemical Biology, 13, 647.","journal-title":"Nature Chemical Biology"},{"key":"2216_CR4","doi-asserted-by":"publisher","first-page":"2897","DOI":"10.7150\/ijbs.30499","volume":"15","author":"X Cheng","year":"2019","unstructured":"Cheng, X., Jiang, X., Tam, K. Y., Li, G., Zheng, J., & Zhang, H. (2019). Sphingolipidomic analysis of C. Elegans\u2009reveals development- and environment-dependent metabolic features. International Journal of Biological Sciences, 15, 2897\u20132910.","journal-title":"International Journal of Biological Sciences"},{"key":"2216_CR5","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1007\/BF02537032","volume":"30","author":"D Chitwood","year":"1995","unstructured":"Chitwood, D., Lusby, W., Thompson, M., Kochansky, J., & Howarth, O. (1995). The glycosylceramides of the nematode Caenorhabditis elegans contain an unusual, branched-chain sphingoid base. Lipids, 30, 567\u2013573.","journal-title":"Lipids"},{"key":"2216_CR6","doi-asserted-by":"publisher","first-page":"S9","DOI":"10.1194\/jlr.R800095-JLR200","volume":"50","author":"E Fahy","year":"2009","unstructured":"Fahy, E., Subramaniam, S., Murphy, R. C., Nishijima, M., Raetz, C. R. H., Shimizu, T., Spener, F., van Meer, G., Wakelam, M. J. O., & Dennis, E. A. (2009). Update of the LIPID MAPS comprehensive classification system for lipids. Journal of Lipid Research, 50, S9\u2013S14.","journal-title":"Journal of Lipid Research"},{"key":"2216_CR7","doi-asserted-by":"publisher","first-page":"342535","DOI":"10.1016\/j.aca.2024.342535","volume":"1304","author":"AC George","year":"2024","unstructured":"George, A. C., Schmitz, I., Rouvi\u00e8re, F., Alves, S., Colsch, B., Heinisch, S., Afonso, C., Fenaille, F., & Loutelier-Bourhis, C. (2024). Interplatform comparison between three ion mobility techniques for human plasma lipid collision cross sections. Analytica Chimica Acta, 1304, 342535.","journal-title":"Analytica Chimica Acta"},{"key":"2216_CR8","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1093\/glycob\/7.2.265","volume":"7","author":"S Gerdt","year":"1997","unstructured":"Gerdt, S., Lochnit, G., Dennis, R. D., & Geyer, R. (1997). Isolation and structural analysis of three neutral glycosphingolipids from a mixed population of Caenorhabditis elegans (Nematoda: Rhabditida). Glycobiology, 7, 265\u2013275.","journal-title":"Glycobiology"},{"key":"2216_CR9","doi-asserted-by":"publisher","first-page":"9193","DOI":"10.1021\/acs.analchem.1c01294","volume":"93","author":"GJ Gouveia","year":"2021","unstructured":"Gouveia, G. J., Shaver, A. O., Garcia, B. M., Morse, A. M., Andersen, E. C., Edison, A. S., & McIntyre, L. M. (2021). Long-term metabolomics reference material. Analytical Chemistry, 93, 9193\u20139199.","journal-title":"Analytical Chemistry"},{"key":"2216_CR10","doi-asserted-by":"publisher","first-page":"e1008638","DOI":"10.1371\/journal.pgen.1008638","volume":"16","author":"S Haeussler","year":"2020","unstructured":"Haeussler, S., K\u00f6hler, F., Witting, M., Premm, M. F., Rolland, S. G., Fischer, C., Chauve, L., Casanueva, O., & Conradt, B. (2020). Autophagy compensates for defects in mitochondrial dynamics. PLOS Genetics, 16, e1008638.","journal-title":"PLOS Genetics"},{"key":"2216_CR11","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.chemphyslip.2019.04.009","volume":"222","author":"V H\u00e4nel","year":"2019","unstructured":"H\u00e4nel, V., Pendleton, C., & Witting, M. (2019). The sphingolipidome of the model organism Caenorhabditis elegans. Chemistry and Physics of Lipids, 222, 15\u201322.","journal-title":"Chemistry and Physics of Lipids"},{"key":"2216_CR12","doi-asserted-by":"publisher","first-page":"3676","DOI":"10.1039\/C6SC04831E","volume":"8","author":"JT Hannich","year":"2017","unstructured":"Hannich, J. T., Mellal, D., Feng, S., Zumbuehl, A., & Riezman, H. (2017). Structure and conserved function of iso-branched sphingoid bases from the nematode Caenorhabditis elegans. Chemical Science, 8, 3676\u20133686.","journal-title":"Chemical Science"},{"key":"2216_CR13","doi-asserted-by":"publisher","first-page":"R8","DOI":"10.1186\/gb-2011-12-1-r8","volume":"12","author":"R Herzog","year":"2011","unstructured":"Herzog, R., Schwudke, D., Schuhmann, K., Sampaio, J. L., Bornstein, S. R., Schroeder, M., & Shevchenko, A. (2011). A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language. Genome Biology, 12, R8.","journal-title":"Genome Biology"},{"key":"2216_CR14","doi-asserted-by":"crossref","unstructured":"Horai, H., Arita, M., Kanaya, S., Nihei, Y., Ikeda, T., Suwa, K., Ojima, Y., Tanaka, K., Tanaka, S., Aoshima, K., Oda, Y., Kakazu, Y., Kusano, M., Tohge, T., Matsuda, F., Sawada, Y., Hirai, M., Nakanishi, H., Ikeda, K., \u2026 Nishioka, T. (2010). MassBank: A public repository for sharing mass spectral data for life sciences. Journal of Mass Spectrometry, 45, 703-714.","DOI":"10.1002\/jms.1777"},{"key":"2216_CR15","doi-asserted-by":"crossref","unstructured":"Jarmusch, A. K., Aron, A. T., Petras, D., Phelan, V. V., Bittremieux, W., Acharya, D. D., Ahmed, M. M. A., Bauermeister, A., Bertin, M. J., Boudreau, P. D., Borges, R. M., Bowen, B. P., Brown, C. J., Chagas, F. O., Clevenger, K. D., Correia, M. S. P., Crandall, W. J., Cr\u00fcsemann, M., Damiani, T., Fiehn, O., Garg, N., Gerwick, W. H., Gilbert, J. R., Globisch, D., Gomes, P. W. P., Heuckeroth, S., James, C. A., Jarmusch, S. A., Kakhkhorov, S. A., Kang, K. B., Kersten, R. D., Kim, H., Kirk, R. D., Kohlbacher, O., Kontou, E. E., Liu, K., Lizama-Chamu, I., Luu, G. T., Knaan, T. L., Marty, M. T., McAvoy, A. C., McCall, L. I., Mohamed, O. G., Nahor, O., Niedermeyer, T. H. J., Northen, T. R., Overdahl, K. E., Pluskal, T., Rainer, J., Reher, R., Rodriguez, E., Sachsenberg, T. T., Sanchez, L. M., Schmid, R., Stevens, C., Tian, Z., Tripathi, A., Tsugawa, H., Nishida, K., Matsuzawa, Y., Hooft, Vicini, A., Walter, A., Weber, T., Xiong, Q., Xu, T., Zhao, H. N., Dorrestein, P. C., & Wang, M. (2022). A universal language for finding mass spectrometry data patterns. bioRxiv, 2022.08.06.503000.","DOI":"10.1101\/2022.08.06.503000"},{"key":"2216_CR16","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1194\/jlr.S120001025","volume":"61","author":"G Liebisch","year":"2020","unstructured":"Liebisch, G., Fahy, E., Aoki, J., Dennis, E. A., Durand, T., Ejsing, C. S., Fedorova, M., Feussner, I., Griffiths, W. J., K\u00f6feler, H., Merrill, A. H. Jr., Murphy, R. C., O\u2019Donnell, V. B., Oskolkova, O., Subramaniam, S., Wakelam, M. J. O., & Spener, F. (2020). Update on LIPID MAPS classification, nomenclature, and shorthand notation for MS-derived lipid structures. Journal of Lipid Research, 61, 1539\u20131555.","journal-title":"Journal of Lipid Research"},{"key":"2216_CR17","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1126\/science.1174621","volume":"327","author":"D Lingwood","year":"2010","unstructured":"Lingwood, D., & Simons, K. (2010). Lipid rafts as a membrane-organizing principle. Science, 327, 46\u201350.","journal-title":"Science"},{"key":"2216_CR18","doi-asserted-by":"publisher","first-page":"1690","DOI":"10.1194\/jlr.D023036","volume":"53","author":"L L\u00f6fgren","year":"2012","unstructured":"L\u00f6fgren, L., St\u00e5hlman, M., Forsberg, G. B., Saarinen, S., Nilsson, R., & Hansson, G. I. (2012). The BUME method: A novel automated chloroform-free 96-well total lipid extraction method for blood plasma. Journal of Lipid Research, 53, 1690\u20131700.","journal-title":"Journal of Lipid Research"},{"key":"2216_CR19","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1194\/jlr.D700041-JLR200","volume":"49","author":"V Matyash","year":"2008","unstructured":"Matyash, V., Liebisch, G., Kurzchalia, T. V., Shevchenko, A., & Schwudke, D. (2008). Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics. Journal of Lipid Research, 49, 1137\u20131146.","journal-title":"Journal of Lipid Research"},{"key":"2216_CR20","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1194\/jlr.M200323-JLR200","volume":"44","author":"M Merris","year":"2003","unstructured":"Merris, M., Wadsworth, W. G., Khamrai, U., Bittman, R., Chitwood, D. J., & Lenard, J. (2003). Sterol effects and sites of sterol accumulation in Caenorhabditis elegans: Developmental requirement for 4\u03b1-methyl sterols. Journal of Lipid Research, 44, 172\u2013181.","journal-title":"Journal of Lipid Research"},{"key":"2216_CR21","doi-asserted-by":"publisher","first-page":"e70087","DOI":"10.1371\/journal.pone.0070087","volume":"8","author":"MB Mosbech","year":"2013","unstructured":"Mosbech, M. B., Kruse, R., Harvald, E. B., Olsen, A. S. B., Gallego, S. F., Hannibal-Bach, H. K., Ejsing, C. S., & F\u00e6rgeman, N. J. (2013). Functional loss of two ceramide synthases elicits autophagy-dependent lifespan extension in C. elegans. Plos One, 8, e70087.","journal-title":"Plos One"},{"key":"2216_CR22","doi-asserted-by":"publisher","first-page":"eaaw2964","DOI":"10.1126\/scisignal.aaw2964","volume":"12","author":"VB O\u2019Donnell","year":"2019","unstructured":"O\u2019Donnell, V. B., Dennis, E. A., Wakelam, M. J. O., & Subramaniam, S. (2019). LIPID MAPS: Serving the next generation of lipid researchers with tools, resources, data, and training. Science Signaling, 12, eaaw2964.","journal-title":"Science Signaling"},{"key":"2216_CR23","doi-asserted-by":"publisher","first-page":"2703","DOI":"10.1021\/ac404083u","volume":"86","author":"C Papan","year":"2014","unstructured":"Papan, C., Penkov, S., Herzog, R., Thiele, C., Kurzchalia, T., & Shevchenko, A. (2014). Systematic screening for novel lipids by shotgun lipidomics. Analytical Chemistry, 86, 2703\u20132710.","journal-title":"Analytical Chemistry"},{"key":"2216_CR37","doi-asserted-by":"crossref","unstructured":"Penkov, S., Mende, F., Zagoriy, V., Erkut, C., Martin, R., P\u00e4ssler, U., Schuhmann, K., Schwudke, D., Gruner, M., M\u00e4ntler, J., Reichert-M\u00fcller, T., Shevchenko, A., Kn\u00f6lker, H. J., & Kurzchalia, T. V. (2010). Maradolipids: diacyltrehalose glycolipids specific to dauer larva in Caenorhabditis elegans. Angewandte Chemie (International ed. in English), 49(49), 9430\u20139435. https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/ange.201004466","DOI":"10.1002\/anie.201004466"},{"key":"2216_CR24","doi-asserted-by":"publisher","first-page":"108653","DOI":"10.1016\/j.celrep.2020.108653","volume":"34","author":"E Rackles","year":"2021","unstructured":"Rackles, E., Witting, M., Forn\u00e9, I., Zhang, X., Zacherl, J., Schrott, S., Fischer, C., Ewbank, J. J., Osman, C., Imhof, A., & Rolland, S. G. (2021). Reduced peroxisomal import triggers peroxisomal retrograde signaling. Cell Reports, 34, 108653.","journal-title":"Cell Reports"},{"key":"2216_CR25","doi-asserted-by":"crossref","unstructured":"Rainer, J., Vicini, A., Salzer, L., Stanstrup, J., Badia, J. M., Neumann, S., Stravs, M. A., Hernandes, V., Gatto, V., Gibb, L., S. and, & Witting, M. (2022). A modular and expandable ecosystem for metabolomics data annotation in R. Metabolites, 12, 173.","DOI":"10.3390\/metabo12020173"},{"key":"2216_CR26","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.jprot.2010.11.001","volume":"74","author":"W Rao","year":"2011","unstructured":"Rao, W., Isaac, R. E., & Keen, J. N. (2011). An analysis of the Caenorhabditis elegans lipid raft proteome using geLC-MS\/MS. Journal of Proteomics, 74, 242\u2013253.","journal-title":"Journal of Proteomics"},{"key":"2216_CR27","doi-asserted-by":"publisher","first-page":"462481","DOI":"10.1016\/j.chroma.2021.462481","volume":"1655","author":"J Scholz","year":"2021","unstructured":"Scholz, J., Helmer, P. O., Nicolai, M. M., Bornhorst, J., & Hayen, H. (2021). Profiling of sphingolipids in Caenorhabditis elegans by two-dimensional multiple heart-cut liquid chromatography: Mass spectrometry. Journal of Chromatography A, 1655, 462481.","journal-title":"Journal of Chromatography A"},{"key":"2216_CR28","doi-asserted-by":"publisher","first-page":"C468","DOI":"10.1152\/ajpcell.00182.2003","volume":"287","author":"MM Sedensky","year":"2004","unstructured":"Sedensky, M. M., Siefker, J. M., Koh, J. Y., Miller, D. M., I. and, & Morgan, P. G. (2004). A stomatin and a degenerin interact in lipid rafts of the nervous system of Caenorhabditis elegans. American Journal of Physiology-Cell Physiology, 287, C468\u2013C474.","journal-title":"American Journal of Physiology-Cell Physiology"},{"key":"2216_CR29","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1038\/42408","volume":"387","author":"K Simons","year":"1997","unstructured":"Simons, K., & Ikonen, E. (1997). Functional rafts in cell membranes. Nature, 387, 569.","journal-title":"Nature"},{"key":"2216_CR30","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.celrep.2016.05.086","volume":"16","author":"LJ Smulan","year":"2016","unstructured":"Smulan, L. J., Ding, W., Freinkman, E., Gujja, S., Edwards, Y. J. K., & Walker, A. K. (2016). Cholesterol-independent SREBP-1 maturation is linked to ARF1 inactivation. Cell Reports, 16, 9\u201318.","journal-title":"Cell Reports"},{"key":"2216_CR31","doi-asserted-by":"publisher","first-page":"D463","DOI":"10.1093\/nar\/gkv1042","volume":"44","author":"M Sud","year":"2016","unstructured":"Sud, M., Fahy, E., Cotter, D., Azam, K., Vadivelu, I., Burant, C., Edison, A., Fiehn, O., Higashi, R., Nair, K. S., Sumner, S., & Subramaniam, S. (2016). Metabolomics Workbench: An international repository for metabolomics data and metadata, metabolite standards, protocols, tutorials and training, and analysis tools. Nucleic Acids Research, 44, D463\u2013D470.","journal-title":"Nucleic Acids Research"},{"key":"2216_CR32","doi-asserted-by":"publisher","first-page":"3159","DOI":"10.1038\/sj.emboj.7600798","volume":"24","author":"G van Meer","year":"2005","unstructured":"van Meer, G. (2005). Cellular lipidomics. Embo Journal, 24, 3159\u20133165.","journal-title":"Embo Journal"},{"key":"2216_CR33","doi-asserted-by":"crossref","unstructured":"Wang, M., Carver, J. J., Phelan, V. V., Sanchez, L. M., Garg, N., Peng, Y., Nguyen, D. D., Watrous, J., Kapono, C. A., Luzzatto-Knaan, T., Porto, C., Bouslimani, A., Melnik, A. V., Meehan, M. J., Liu, W. T., Cr\u00fcsemann, M., Boudreau, P. D., Esquenazi, E., Sandoval-Calder\u00f3n, M., Kersten, R. D., Pace, L. A., Quinn, R. A., Duncan, K. R., Hsu, C. C., Floros, D. J., Gavilan, R. G., Kleigrewe, K., Northen, T., Dutton, R. J., Parrot, D., Carlson, E. E., Aigle, B., Michelsen, C. F., Jelsbak, L., Sohlenkamp, C., Pevzner, P., Edlund, A., McLean, J., Piel, J., Murphy, B. T., Gerwick, L., Liaw, C. C., Yang, Y. L., Humpf, H. U., Maansson, M., Keyzers, R. A., Sims, A. C., Johnson, A. R., Sidebottom, A. M., Sedio, B. E., Klitgaard, A., Larson, C. B., Boya, P., Torres-Mendoza, C. A., Gonzalez, D., Silva, D. J., Marques, D. B., Demarque, L. M., Pociute, D. P., O\u2019Neill, E., Briand, E. C., Helfrich, E., Granatosky, E. J. N., Glukhov, E. A., Ryffel, E., Houson, F., Mohimani, H., Kharbush, H., Zeng, J. J., Vorholt, Y., Kurita, J. A., Charusanti, K. L., McPhail, P., Nielsen, K. L., Vuong, K. F., Elfeki, L., Traxler, M., Engene, M. F., Koyama, N., Vining, N., Baric, O. B., Silva, R., Mascuch, R. R., Tomasi, S. J., Jenkins, S., Macherla, S., Hoffman, V., Agarwal, T., Williams, V., Dai, P. G., Neupane, J., Gurr, R., Rodr\u00edguez, J., Lamsa, A. M. C., Zhang, A., Dorrestein, C., Duggan, K. (2016). B.M., Almaliti, J., Allard, P.-M., Phapale, P. Sharing and community curation of mass spectrometry data with global natural products social molecular networking. Nature Biotechnology, 34, 828.","DOI":"10.1038\/nbt.3597"},{"key":"2216_CR34","first-page":"413","volume":"207","author":"JL Watts","year":"2017","unstructured":"Watts, J. L., & Ristow, M. (2017). Lipid and carbohydrate metabolism in Caenorhabditis elegans. Genetics, 207, 413\u2013446.","journal-title":"Genetics"},{"key":"2216_CR35","doi-asserted-by":"crossref","unstructured":"Witting, M., Maier, T.V., Garvis, S. and Schmitt-Kopplin, P. (2014) Optimizing a ultrahigh pressure liquid chromatography-time of flight-mass spectrometry approach using a novel sub-2\u00a0\u00b5m core\u2013shell particle for in depth lipidomic profiling of Caenorhabditis elegans.Journal of Chromatography A, 1359, 91\u201399.","DOI":"10.1016\/j.chroma.2014.07.021"},{"key":"2216_CR36","doi-asserted-by":"publisher","first-page":"2091","DOI":"10.1007\/s00216-021-03172-3","volume":"413","author":"M Witting","year":"2021","unstructured":"Witting, M., Schmidt, U., & Kn\u00f6lker, H. J. (2021). UHPLC-IM-Q-ToFMS analysis of maradolipids, found exclusively in Caenorhabditis elegans dauer larvae. Analytical and Bioanalytical Chemistry, 413, 2091\u20132102.","journal-title":"Analytical and Bioanalytical Chemistry"}],"container-title":["Metabolomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11306-024-02216-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11306-024-02216-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11306-024-02216-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T05:19:31Z","timestamp":1745039971000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11306-024-02216-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,20]]},"references-count":37,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["2216"],"URL":"https:\/\/doi.org\/10.1007\/s11306-024-02216-w","relation":{},"ISSN":["1573-3890"],"issn-type":[{"value":"1573-3890","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,20]]},"assertion":[{"value":"24 September 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 December 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 February 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The following authors state that they have potential conflicts of interest regarding this work: A.B. and S.W.M. are employees of Bruker Corporation or one of its subsidiaries (\u201cBruker\u201d). Bruker manufactures and sells analytical instruments including mass spectrometers and software. Bruker mass Spectrometers, LC systems and software were used in this study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"29"}}