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Locating the position of double bonds is vital since minor structural differences between positional isomers can alter a lipid\u2019s biochemical function.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Objectives<\/jats:title>\n            <jats:p>Oxygen Attachment Dissociation (OAD) is a novel fragmentation technology that enables the localisation of C\u2009=\u2009C double bonds in lipids. To evaluate its use in the structural characterisation of lipids, OAD has been applied in a discovery-based untargeted analysis of the metabolic impact of acute ethanol exposure in a mouse model.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>UHPLC-OAD-MS\/MS was used to enhance the identification of lipids found to be significantly altered by acute ethanol exposure in the gut, liver and pancreas tissues of male C57BL\/6 mice receiving a Lieber-DeCarli liquid diet either containing 5% ethanol or an isocaloric control diet. Tissue extracts were analysed using untargeted UHPLC-DIA-MS\/MS; UHPLC-OAD-MS\/MS analysis was performed to further annotate lipids that were significantly increased or diminished in the animals exposed to ethanol.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>UHPLC-DIA-MS\/MS analysis of gut, liver and pancreas tissue revealed 101 lipids that were significantly increased or diminished in ethanol treated mice. Of the included 83 unsaturated lipids detected, UHPLC-OAD-MS\/MS enabled the localisation of C\u2009=\u2009C double bonds in 61, including isomers indistinguishable by MS\/MS with collision induced dissociation.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The results demonstrate the value of OAD-MS\/MS in enhancing lipid identification. The resulting improvement may enable better understanding of the underlying biochemistry in the response of mice to exposure to ethanol.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s11306-025-02282-8","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T17:15:13Z","timestamp":1751390113000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Oxygen attachment dissociation (OAD) MS\/MS in the identification of positional isomers of dysregulated lipids detected in an ethanol exposure metabolomics study in mice"],"prefix":"10.1007","volume":"21","author":[{"given":"Emily G.","family":"Armitage","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Barnes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Deda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Virgiliou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil J.","family":"Loftus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Gika","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian D.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"issue":"5","key":"2282_CR1","doi-asserted-by":"publisher","first-page":"858","DOI":"10.1194\/jlr.M067199","volume":"57","author":"T Baba","year":"2016","unstructured":"Baba, T., Campbell, J. 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