{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T21:00:48Z","timestamp":1758056448019,"version":"3.44.0"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T00:00:00Z","timestamp":1753574400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T00:00:00Z","timestamp":1753574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Metabolomics"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Introduction<\/jats:title>\n            <jats:p>Metabolic processes play a role in cancer development, with faecal amino acids emerging as potential biomarkers for colorectal neoplasia. While fresh frozen tissue is preferred for metabolomic analysis, formalin-fixed paraffin-embedded (FFPE) tissue is more widely available.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Objectives<\/jats:title>\n            <jats:p>We aimed to evaluate amino acid profiles in FFPE tissue across different stages of the adenoma-carcinoma sequence.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Method<\/jats:title>\n            <jats:p>A panel of 20 amino acids was measured using liquid chromatography\u2013tandem mass spectrometry.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Fourteen amino acids were detected, with proline elevated in colorectal carcinoma versus advanced (FC 2.33, <jats:italic>p<\/jats:italic>\u2009=\u20090.04) and non-advanced adenomas (FC 2.42, <jats:italic>p<\/jats:italic>\u2009=\u20090.02).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Despite analytical challenges, amino acid profiling in FFPE tissue is feasible.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s11306-025-02301-8","type":"journal-article","created":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T06:52:58Z","timestamp":1753599178000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Feasibility of amino acid profiling in long-term stored formalin-fixed paraffin-embedded colorectal neoplasia tissue"],"prefix":"10.1007","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7279-0940","authenticated-orcid":false,"given":"Roza C. 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H.","family":"de Boer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduard A.","family":"Struys","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,27]]},"reference":[{"key":"2301_CR1","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1158\/1541-7786.Mcr-19-1091","volume":"18","author":"K Arima","year":"2020","unstructured":"Arima, K., Lau, M. C., Zhao, M., Haruki, K., Kosumi, K., Mima, K., Gu, M. C., V\u00e4yrynen, J. P., Twombly, T. S., Baba, Y., Fujiyoshi, K., Kishikawa, J., Guo, C. G., Baba, H., Richards, W. G., Chan, A. T., Nishihara, R., Meyerhardt, J. A., Nowak, J. A., Giannakis, M., Fuchs, C. S., & Ogino, S. (2020). Metabolic profiling of formalin-fixed paraffin-embedded tissues discriminates normal colon from colorectal cancer. 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He has received a research grant (unrestricted) from Dr. Falk, TEVA PharmaBV, Dutch Digestive Foundation (MLDS) and Takeda. All outside the submitted work. All other authors declare no conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and\/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Consent for the retrieval of participants\u2019 tissue was obtained through an opt-out form.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"102"}}