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Additionally, seed pods and other plant tissues are considered edible and are used as green vegetables in many parts of the world.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Flavonol glycosides are well-known in faba bean leaves; accordingly, in this study, we followed a targeted metabolomic approach to explore glycosylated flavonols and their concentrations in response to contrasting levels of selenium (Se) and sulfur (S) enrichment under faba bean\u2013\n                      <jats:italic>Rhizobium<\/jats:italic>\n                      symbiosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Faba bean plants were cultivated under growth chamber conditions and enriched with different levels of selenium and sulfur under\n                      <jats:italic>Rhizobium<\/jats:italic>\n                      inoculation. Their leaves were extracted using 70% methanol to quantify glycosylated flavonoids. Sample leaves were analyzed through a targeted method using high-performance liquid chromatography combined with a diode array detector (HPLC-DAD) and electrospray ionization-quadrupole-time-of-flight tandem mass spectrometry detection (HPLC-ESI-Q-ToF-MS\/MS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The analysis led to semi-quantifying 11 flavonol glycosides. Analysis of the metabolites of the different faba bean leaf extracts confirmed that selenium has a considerable impact on the accumulation of flavonol glycosides, especially under sulfur availability, possibly because it induces chalcone synthase and other enzymes for flavonols\u2019 biosynthesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      To the best of our knowledge, this is the first report to investigate the impact of selenium and sulfur enrichment on the accumulation of faba bean flavonols under atmospheric nitrogen (N\n                      <jats:sub>2<\/jats:sub>\n                      ) fixation conditions. This study highlights the medicinal and nutritional benefits of legumes as an essential source of protein in plant-based foods.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11306-025-02313-4","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T11:58:55Z","timestamp":1754999935000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Flavonol glycosides accumulation in faba bean grown under combined selenium and sulfur application"],"prefix":"10.1007","volume":"21","author":[{"given":"Muna Ali","family":"Abdalla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khuram","family":"Waqas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Neugart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl Hermann","family":"M\u00fchling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"key":"2313_CR1","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1080\/07352689.2022.2158270","volume":"42","author":"MA Abdalla","year":"2023","unstructured":"Abdalla, M. 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