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All animals grew normally and their urine was collected daily. Three control mice were treated in the same way, but with an unenriched diet. LC-HRMS-based metabolomics profiling were conducted on all collected samples as well as isotope tracing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Comparative LC-HRMS analysis of the\n                      <jats:sup>12<\/jats:sup>\n                      C- and\n                      <jats:sup>13<\/jats:sup>\n                      C-samples unambiguously identified 238 metabolites, whose\n                      <jats:sup>13<\/jats:sup>\n                      C-labeling profiles were then studied over a 39-day period. Although overall urine labeling was fast and rapidly reached a high level of\n                      <jats:sup>13<\/jats:sup>\n                      C-content (&gt;\u200990% after 22 days), isotopic monitoring of each molecular species demonstrated that\n                      <jats:sup>13<\/jats:sup>\n                      C-incorporation kinetics are considerably variable between metabolites, reflecting their biological function, origin and rate of biosynthesis in vivo.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study demonstrates that mice tolerate a diet entirely labeled with carbon-13 (&gt;\u200997 atom %\n                      <jats:sup>13<\/jats:sup>\n                      C) over a period of six-weeks. It reveals the dynamics of a large part of the metabolome at the mammalian species level through urine analysis coupled to in vivo\n                      <jats:sup>13<\/jats:sup>\n                      C-labeling. The study provides valuable insights by comprehensively covering metabolic pathways, and stands out from targeted fluxomic studies which are carried out by administering a very limited number of specific labeled precursors.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11306-025-02391-4","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T16:13:51Z","timestamp":1767024831000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Urinary metabolome dynamics in 13C-labeled mice"],"prefix":"10.1007","volume":"22","author":[{"given":"Annelaure","family":"Damont","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana\u00efs","family":"Legrand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen","family":"Rousseau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Bellanger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9verine","family":"Boiry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Gibiat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Jacques","family":"Leguay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Junot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Fenaille","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Ezan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,29]]},"reference":[{"issue":"8","key":"2391_CR1","doi-asserted-by":"publisher","first-page":"955","DOI":"10.3390\/bioengineering10080955","volume":"10","author":"AP Abreu","year":"2023","unstructured":"Abreu, A. 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